The Experts below are selected from a list of 104130 Experts worldwide ranked by ideXlab platform
Claude M Fauquet - One of the best experts on this subject based on the ideXlab platform.
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application of fta technology for sampling recovery and molecular characterization of viral pathogens and virus derived transgenes from Plant Tissues
Virology Journal, 2005Co-Authors: Joseph Ndunguru, Nigel J Taylor, Jitender S Yadav, J P Legg, Terry Aveling, Graham Thompson, Claude M FauquetAbstract:Background Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting Plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled Plant Tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh Tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from Plant Tissues and their subsequent molecular analysis.
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application of fta technology for sampling recovery and molecular characterization of viral pathogens and virus derived transgenes from Plant Tissues
Virology Journal, 2005Co-Authors: Joseph Ndunguru, Nigel J Taylor, Jitender S Yadav, J P Legg, Terry Aveling, Graham Thompson, Haytham Aly, Claude M FauquetAbstract:Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting Plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled Plant Tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh Tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from Plant Tissues and their subsequent molecular analysis. DNA and RNA viruses were successfully recovered from leaf Tissues of maize, cassava, tomato and tobacco pressed into FTA® Classic Cards. Viral nucleic acids eluted from FTA cards were found to be suitable for diagnostic molecular analysis by PCR-based techniques and restriction analysis, and for cloning and nucleotide sequencing in a manner equivalent to that offered by tradition isolation methods. Efficacy of the technology was demonstrated both from sampled greenhouse-grown Plants and from leaf presses taken from crop Plants growing in farmer's fields in East Africa. In addition, FTA technology was shown to be suitable for recovery of viral-derived transgene sequences integrated into the Plant genome. Results demonstrate that FTA is a practical, economical and sensitive method for sampling, storage and retrieval of viral pathogens and Plant genomic sequences, when working under controlled conditions and in the field. Application of this technology has the potential to significantly increase ability to bring modern analytical techniques to bear on the viral pathogens infecting crop Plants.
William F Thompson - One of the best experts on this subject based on the ideXlab platform.
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a modified protocol for rapid dna isolation from Plant Tissues using cetyltrimethylammonium bromide
Nature Protocols, 2006Co-Authors: George C Allen, Miguel A Floresvergara, S Krasynanski, Sandeep Kumar, William F ThompsonAbstract:We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from Plant Tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on Plant species and tissue source. It can be completed in as little as 5-6 h.
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a modified protocol for rapid dna isolation from Plant Tissues using cetyltrimethylammonium bromide
Nature Protocols, 2006Co-Authors: George C Allen, Miguel A Floresvergara, S Krasynanski, Sandeep Kumar, William F ThompsonAbstract:We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from Plant Tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5–30 μg of total DNA from 200 mg of tissue fresh weight, depending on Plant species and tissue source. It can be completed in as little as 5–6 h. Note: In the version of this article initially published online, the name of the coauthor S Krasnyanski was misspelled as S Krasynanski. This error has been corrected in all versions of the article.
Joseph Ndunguru - One of the best experts on this subject based on the ideXlab platform.
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application of fta technology for sampling recovery and molecular characterization of viral pathogens and virus derived transgenes from Plant Tissues
Virology Journal, 2005Co-Authors: Joseph Ndunguru, Nigel J Taylor, Jitender S Yadav, J P Legg, Terry Aveling, Graham Thompson, Claude M FauquetAbstract:Background Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting Plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled Plant Tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh Tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from Plant Tissues and their subsequent molecular analysis.
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application of fta technology for sampling recovery and molecular characterization of viral pathogens and virus derived transgenes from Plant Tissues
Virology Journal, 2005Co-Authors: Joseph Ndunguru, Nigel J Taylor, Jitender S Yadav, J P Legg, Terry Aveling, Graham Thompson, Haytham Aly, Claude M FauquetAbstract:Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting Plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled Plant Tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh Tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from Plant Tissues and their subsequent molecular analysis. DNA and RNA viruses were successfully recovered from leaf Tissues of maize, cassava, tomato and tobacco pressed into FTA® Classic Cards. Viral nucleic acids eluted from FTA cards were found to be suitable for diagnostic molecular analysis by PCR-based techniques and restriction analysis, and for cloning and nucleotide sequencing in a manner equivalent to that offered by tradition isolation methods. Efficacy of the technology was demonstrated both from sampled greenhouse-grown Plants and from leaf presses taken from crop Plants growing in farmer's fields in East Africa. In addition, FTA technology was shown to be suitable for recovery of viral-derived transgene sequences integrated into the Plant genome. Results demonstrate that FTA is a practical, economical and sensitive method for sampling, storage and retrieval of viral pathogens and Plant genomic sequences, when working under controlled conditions and in the field. Application of this technology has the potential to significantly increase ability to bring modern analytical techniques to bear on the viral pathogens infecting crop Plants.
George C Allen - One of the best experts on this subject based on the ideXlab platform.
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a modified protocol for rapid dna isolation from Plant Tissues using cetyltrimethylammonium bromide
Nature Protocols, 2006Co-Authors: George C Allen, Miguel A Floresvergara, S Krasynanski, Sandeep Kumar, William F ThompsonAbstract:We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from Plant Tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on Plant species and tissue source. It can be completed in as little as 5-6 h.
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a modified protocol for rapid dna isolation from Plant Tissues using cetyltrimethylammonium bromide
Nature Protocols, 2006Co-Authors: George C Allen, Miguel A Floresvergara, S Krasynanski, Sandeep Kumar, William F ThompsonAbstract:We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from Plant Tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5–30 μg of total DNA from 200 mg of tissue fresh weight, depending on Plant species and tissue source. It can be completed in as little as 5–6 h. Note: In the version of this article initially published online, the name of the coauthor S Krasnyanski was misspelled as S Krasynanski. This error has been corrected in all versions of the article.
Huanwen Chen - One of the best experts on this subject based on the ideXlab platform.
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direct assessment of phytochemicals inherent in Plant Tissues using extractive electrospray ionization mass spectrometry
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Nannan Wang, Konstantin Chingin, Huanwen ChenAbstract:An ambient pressure ionization mass spectrometric strategy called internal extractive electrospray ionization mass spectrometry (iEESI-MS) has been developed and applied for direct profiling of labile phytochemicals inherent in various native Plant Tissues, including leaves, roots, and fruits. By passing the electrospray solvent through the Plant tissue, a variety of phytochemicals, such as amino acids, sugars (e.g., glucose, sucrose, polysaccharides, etc.), and alkaloids, were continuously extracted from the sample interior, driven toward the natural/cut electro-spraying tip, and vaporized into gaseous ions for mass spectrometric interrogation. Phytochemical patterns obtained by iEESI–MS permit a rapid differentiation between various species of ginkgo Plant and strawberry maturity stages, as well as characterization of physiological/pathologic conditions of chlorophytum comosum. Our experimental results further demonstrate that the established iEESI–MS approach is potentially useful for direct phytochemo...
