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8 Isoprostane

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Paolo Montuschi – One of the best experts on this subject based on the ideXlab platform.

Giovanni Ciabattoni – One of the best experts on this subject based on the ideXlab platform.

  • 8Isoprostane in exhaled breath condensate after experimental exposure to wood smoke in humans.
    Journal of biological regulators and homeostatic agents, 2016
    Co-Authors: Nicola Murgia, Paolo Montuschi, Giovanni Ciabattoni, Lars Barregard, Gerd Sallsten, A. C. Almstrand, Anna-carin Olin
    Abstract:

    Wood smoke, a well-known indoor and outdoor air pollutant, may cause adverse health effects through oxidative stress. In this study 8Isoprostane, a biomarker of oxidative stress, was measured in exhaled breath condensate (EBC) and urine before and after experimental exposure to wood smoke. The results were compared with measurements of other biomarkers of oxidative stress and inflammation. Thirteen subjects were exposed first to clean air and then, after 1 week, to wood smoke in an exposure chamber during 4-hour sessions. Exhaled breath condensate, exhaled nitric oxide, blood and urine were sampled before and at various intervals after exposure to wood smoke and clean air. Exhaled breath condensate was examined for 8Isoprostane and malondialdehyde (MDA), while exhaled air was examined for nitric oxide, serum for Clara cell protein (CC16) and urine for 8Isoprostane. 8Isoprostane in EBC did not increase after wood smoke exposure and its net change immediately after exposure was inversely correlated with net changes in MDA (r(s)= -0.57, p= 0.041) and serum CC16 (S-CC16) (r(p)= -0.64, p= 0.020) immediately after the exposure. No correlation was found between 8Isoprostane in urine and 8Isoprostane in EBC. In this study controlled wood smoke exposure in healthy subjects did not increase 8Isoprostane in EBC.

  • 8Isoprostane in exhaled breath condensate (EBC) and air pollution exposure in children with wheezing
    European Respiratory Journal, 2012
    Co-Authors: Pedro Martins, Paolo Montuschi, Giovanni Ciabattoni, Iolanda Caires, José Araújo-martins, Joana Valente, Myriam Lopes, Carlos Borrego, Nuno Neuparth
    Abstract:

    Background: Oxidative stress is proposed as the underlying mechanism of air pollutants aggression over the airways. 8Isoprostane is a reliable biomarker of oxidative stress. 8Isoprostane could be detected in several fluids including exhaled breath condensate (EBC). Objective: To study the relation between 8Isoprostane in EBC and air pollution exposure. Methods : In the scope of a prospective study, EBC samples were collected from 27 wheezing children in order to measure 8Isoprostanes. Children were also evaluated through spirometry and skin prick tests for airborne allergens. After the definition of a day activity pattern for each children and direct measurements of air pollutants in different microenvironments (home, school and outdoor), individual exposure was calculated for PM 10, O 3 , NO 2 , xylene, toluene, benzene, formaldehyde and ethylbenzene. Spearman rank correlation was used to study the associations between 8Isoprostane and air pollutants. Results: The mean age of the studied children was 7.9 ± 1.1 years. Eleven were boys. The mean FEV 1 was 96.7 ± 9.6%. Ten of the studied children were atopic. Exposure to volatile organic compounds (VOCs) including toluene (rho = 0,604, p = 0,008), xylene (rho = 0,685, p = 0,002) and ethylbenzene (rho = 0,788, p 10, O 3 , NO 2 neither between EBC 8Isoprostane and spirometric results. Conclusions: Individual exposure to VOCs seems to be related with oxidative stress evaluated through 8Isoprostanes measurement in EBC. Granted by : Fundacao Calouste Gulbenkian, SaudAr Project.

  • Measurement of 8Isoprostane in exhaled breath condensate.
    Methods in molecular biology (Clifton N.J.), 2009
    Co-Authors: Paolo Montuschi, Peter J. Barnes, Giovanni Ciabattoni
    Abstract:

    Oxidative stress is functionally involved in the pathophysiology of lung diseases including asthma and chronic obstructive pulmonary disease. 8Isoprostane, which is derived from free radical-catalyzed peroxidation of arachidonic acid, is one of the most reliable biomarkers of oxidative stress. Exhaled breath condensate (EBC) is a completely noninvasive method for collecting airway secretions. We developed a specific and sensitive radioimmunoassay (RIA) that has been applied to the measurement of 8Isoprostane in EBC. This RIA for 8Isoprostane has been validated using high performance liquid chromatography. Measurement of 8Isoprostane in EBC is a useful noninvasive technique for exploring the role of oxidative stress in lung diseases. This technique might provide important insights into the understanding of the clinical pharmacology of antioxidants and might be useful for monitoring the effects of pharmacological therapy.

Peter J. Barnes – One of the best experts on this subject based on the ideXlab platform.

  • Measurement of 8Isoprostane in exhaled breath condensate.
    Methods in molecular biology (Clifton N.J.), 2009
    Co-Authors: Paolo Montuschi, Peter J. Barnes, Giovanni Ciabattoni
    Abstract:

    Oxidative stress is functionally involved in the pathophysiology of lung diseases including asthma and chronic obstructive pulmonary disease. 8Isoprostane, which is derived from free radical-catalyzed peroxidation of arachidonic acid, is one of the most reliable biomarkers of oxidative stress. Exhaled breath condensate (EBC) is a completely noninvasive method for collecting airway secretions. We developed a specific and sensitive radioimmunoassay (RIA) that has been applied to the measurement of 8Isoprostane in EBC. This RIA for 8Isoprostane has been validated using high performance liquid chromatography. Measurement of 8Isoprostane in EBC is a useful noninvasive technique for exploring the role of oxidative stress in lung diseases. This technique might provide important insights into the understanding of the clinical pharmacology of antioxidants and might be useful for monitoring the effects of pharmacological therapy.

  • exhaled 8 Isoprostane and prostaglandin e2 in patients with stable and unstable cystic fibrosis
    Free Radical Biology and Medicine, 2008
    Co-Authors: Vincenzina Lucidi, Peter J. Barnes, Giovanni Ciabattoni, S Bella, Paolo Montuschi
    Abstract:

    We measured 8Isoprostane, a biomarker of oxidative stress, and prostaglandin (PG) E2 in exhaled breath condensate in 36 stable and 14 unstable cystic fibrfibrosis (CF) patients, and in 15 healthy age-matched controls. We studied the relationships of these eicosanoids with clinical, radiological, and systemic inflammatory parameters. Compared with controls [15.5 (11.5–17.0) pg/ml] exhaled 8Isoprostane was increased in stable CF patients [30.5 (25.3–36.0) pg/ml, P < 0.001]. Unstable CF patients had higher exhaled 8Isoprostane levels [47.5 (44.0–50.0) pg/ml, P < 0.001] than stable CF patients. Unlike PGE2, exhaled 8Isoprostane was negatively correlated with FEV1 (r = −0.67; P < 0.0001; r = −0.63; P < 0.02) and Shwachman score (r = −0.43, P = 0.012; r = −0.58, P = 0.031) and positively correlated with Chrispin-Norman score (r = 0.51, P < 0.002; r = 0.56, P = 0.039) in stable and unstable CF patients, respectively. No correlation was observed with C-reactive protein. Compared with controls [41.0 (29.0–50.0) pg/ml], exhaled PGE2 was also elevated in stable [72.0 (64.3–81.8) pg/ml, P < 0.001) and, to a greater extent, in unstable CF patients [83.0 (74.3–91.3) pg/ml, P < 0.001). In patients with CF, exhaled 8Isoprostane and PGE2 could be a useful marker of disease severity.

  • Exhaled 8Isoprostane and prostaglandin E2 in patients with stable and unstable cystic fibrosis
    Free radical biology & medicine, 2008
    Co-Authors: Vincenzina Lucidi, Peter J. Barnes, Giovanni Ciabattoni, S Bella, Paolo Montuschi
    Abstract:

    We measured 8Isoprostane, a biomarker of oxidative stress, and prostaglandin (PG) E2 in exhaled breath condensate in 36 stable and 14 unstable cystic fibrfibrosis (CF) patients, and in 15 healthy age-matched controls. We studied the relationships of these eicosanoids with clinical, radiological, and systemic inflammatory parameters. Compared with controls [15.5 (11.5–17.0) pg/ml] exhaled 8Isoprostane was increased in stable CF patients [30.5 (25.3–36.0) pg/ml, P 

Sergei A Kharitonov – One of the best experts on this subject based on the ideXlab platform.

  • Exhaled 8Isoprostane in childhood asthma.
    Respiratory research, 2005
    Co-Authors: Sukhbir K. Shahid, Sergei A Kharitonov, Nicola Wilson, Andrew Bush, Peter J. Barnes
    Abstract:

    Background: Exhaled breath condensate (EBC) is a non-invasive method to assess airway inflammation and oxidative stress and may be useful in the assessment of childhood asthma. Methods: Exhaled 8Isoprostane, a stable marker of oxidative stress, was measured in EBC, in children (5–17 years) with asthma (13 steroid-naive and 12 inhaled steroid-treated) and 11 healthy control. Results: Mean exhaled 8Isoprostane concentration was significantly elevated in steroid-naive asthmatic children compared to healthy children 9.3 (SEM 1.7) vs. 3.8 (0.6) pg/ml, p < 0.01. Children on inhaled steroids also had significantly higher 8Isoprostane levels than those of normal subjects 6.7 (0.7) vs. 3.8 (0.6) pg/ml, p < 0.01. Steroid-naive asthmatics had higher exhaled nitric oxide (eNO) than those of controls 28.5 (4.7) vs. 12.6 (1.5) ppb, p < 0.01. eNO in steroid-treated asthmatics was similar to control subjects 27.5(8.8) vs. 12.6(1.5) ppb. Exhaled 8Isoprostane did not correlate with duration of asthma, dose of inhaled steroids or eNO. Conclusion: We conclude that 8Isoprostane is elevated in asthmatic children, indicating increased oxidative stress, and that this does not appear to be normalized by inhaled steroid therapy. This suggests that 8Isoprostane is a useful non-invasive measurement of oxidative stress in children and that antioxidant therapy may be useful in the future.

  • ozone induced increase in exhaled 8 Isoprostane in healthy subjects is resistant to inhaled budesonide
    Free Radical Biology and Medicine, 2002
    Co-Authors: Paolo Montuschi, Sergei A Kharitonov, Julia A Nightingale, Peter J. Barnes
    Abstract:

    The aim of this study was to quantify lung oxidant stress after short-term ozone exposure as reflected by 8Isoprostane concentrations in exhaled breath condensate (EBC) and to investigate the effects of inhaled budesonide on this response. 8Isoprostane is a prostaglandin-F(2 alpha) isomer that is formed in vivo by free radical-catalyzed peroxidation of arachidonic acid. EBC is a noninvasive method to collect airway secretions. We undertook a double-blind, randomized, placebo-controlled, crossover study with inhaled budesonide (800 microg) or placebo twice daily for 2 weeks prior to ozone exposure (400 parts per billion) for 2 h in nine healthy nonsmokers. Exhaled 8Isoprostane was measured by an enzyme immunoassay. 8Isoprostane was increased 4 h after ozone exposure compared to pre-exposure values in both placebo (36.9 +/- 3.9 pg/ml, mean +/- SEM, vs. 16.9 +/- 0.7 pg/ml; p <.001) and budesonide groups (33.4 +/- 2.6 pg/ml vs. 15.8 +/- 0.3 pg/ml; p <.001). Pretreatment with budesonide did not affect the increases in 8Isoprostane (mean differences 3.4 pg/ml, 95% CI –8.9 to 15.7, p =.54). Short-term ozone exposure causes acute increase in lung oxidative stress as reflected by exhaled 8Isoprostane. This increase is resistant to pretreatment with a high dose of inhaled budesonide.

  • Ozone-induced increase in exhaled 8Isoprostane in healthy subjects is resistant to inhaled budesonide.
    Free radical biology & medicine, 2002
    Co-Authors: Paolo Montuschi, Sergei A Kharitonov, Julia A Nightingale, Peter J. Barnes
    Abstract:

    The aim of this study was to quantify lung oxidant stress after short-term ozone exposure as reflected by 8Isoprostane concentrations in exhaled breath condensate (EBC) and to investigate the effects of inhaled budesonide on this response. 8Isoprostane is a prostaglandin-F(2 alpha) isomer that is formed in vivo by free radical-catalyzed peroxidation of arachidonic acid. EBC is a noninvasive method to collect airway secretions. We undertook a double-blind, randomized, placebo-controlled, crossover study with inhaled budesonide (800 microg) or placebo twice daily for 2 weeks prior to ozone exposure (400 parts per billion) for 2 h in nine healthy nonsmokers. Exhaled 8Isoprostane was measured by an enzyme immunoassay. 8Isoprostane was increased 4 h after ozone exposure compared to pre-exposure values in both placebo (36.9 +/- 3.9 pg/ml, mean +/- SEM, vs. 16.9 +/- 0.7 pg/ml; p

Massimo Corradi – One of the best experts on this subject based on the ideXlab platform.

  • EIA and GC/MS analysis of 8Isoprostane in EBC of children with problematic asthma
    The European respiratory journal, 2009
    Co-Authors: Silvia Carraro, Massimo Corradi, Paola Cogo, I. Isak, Manuela Simonato, Virgilio P. Carnielli, Eugenio Baraldi
    Abstract:

    Asthmatic airways are characterised by enhanced oxidative stress, which can be studied by measuring biomarkers, such as 8Isoprostane. The aims of the present study were: 1) to measure the concentrations of 8Isoprostane in exhaled breath condensate (EBC) and urine of children with problematic and well-controlled asthma; 2) to compare the concentrations of 8Isoprostane measured by gas chromatographic/negative ion chemical ionisation mass spectrometry (GC/NICI-MS) and by an enzymatic immunoassay (EIA). We recruited 20 asthmatic allergic children, 13 with well-controlled asthma and seven with problematic asthma. They underwent exhaled nitric oxide measurements and spirometry, and both EBC and urine samples were collected. 8Isoprostane was measured in EBC by GC/NICI-MS and EIA. 8Isoprostane concentrations in EBC were significantly higher in children with problematic asthma than in children with well-controlled asthma (p = 0.01). An acceptable reproducibility emerged between GC/NICI-MS and EIA (coefficient of reproducibility 11.5 pg x mL(-1)). 8Isoprostane levels measured in urine did not correlate with those measured in EBC. We showed that 8Isoprostane in EBC was significantly increased in children with problematic asthma, suggesting a role for oxidative stress in this asthma phenotype. In addition we found an acceptable reproducibility of EIA compared to GC/NICI-MS, even if the latter method had higher accuracy.

  • leukotrienes and 8 Isoprostane in exhaled breath condensate of children with stable and unstable asthma
    The Journal of Allergy and Clinical Immunology, 2004
    Co-Authors: Stefania Zanconato, Silvia Carraro, Rossella Alinovi, Giorgio Piacentini, F Zacchello, Massimo Corradi, Maria Francesca Pasquale, Eugenio Baraldi
    Abstract:

    Abstract Background Cysteinyl-leukotrienes (cys-LTs) and 8Isoprostane are biomarkers of airway inflammation and oxidative stress. Objective The aim of this study was to evaluate cys-LT and 8Isoprostane levels in exhaled breath condensate (EBC) of children with different degrees of asthma severity. Methods EBC was collected from 14 steroid-naive children with mild persistent asthma, 13 children with stable mild- to-moderate persistent asthasthma treated with inhaled corticosteroids (ICS), 9 ICS-treated children with unstable asthma, and 19 healthy children. Results In the three groups of asthmatic children, EBC concentrations of cys-LTs and 8Isoprostane were significantly higher than in control children (steroid-naive asthmatic children: cys-LTs median, 10.8 pg/mL, P P P P P P P NO levels were significantly higher in steroid-naive and in children with unstable asthma compared with ICS-treated children with stable asthma ( P Conclusions Our study shows that EBC cys-LTs and 8Isoprostane concentrations are higher in asthmatic children than in healthy control children, with scattered values in patients with unstable asthma. These findings suggest that EBC eicosanoid measurement may have useful clinical implications for investigating phenotype differences among asthmatic patients.

  • exhaled 8 Isoprostane as an in vivo biomarker of lung oxidative stress in patients with copd and healthy smokers
    American Journal of Respiratory and Critical Care Medicine, 2000
    Co-Authors: Paolo Montuschi, Sergei A Kharitonov, Giovanni Ciabattoni, Massimo Corradi, J V Collins, N Lazzeri, Peter J. Barnes
    Abstract:

    Most of the studies linking chronic obstructive pulmonary disease (COPD) with oxidative stress are in vitro, using invasive techniques, or measuring systemic oxidative stress. The aim of this study was to quantify oxidative stress in the lungs in patients with COPD and in healthy smokers, as reflected by 8Isoprostane concentrations in breath condensate. This is a noninvasive method to collect airway secretions. 8Isoprostane is a prostaglandin-F(2alpha) isomer that is formed in vivo by free radical-catalyzed peroxidation of arachidonic acid. We also studied the acute effect of smoking on exhaled 8Isoprostane in healthy smokers. Exhaled 8Isoprostane was measured by a specific enzyme immunoassay in 10 healthy nonsmokers and 12 smokers, 25 COPD ex-smokers, and 15 COPD current smokers. 8Isoprostane concentrations were similar in COPD ex-smokers (40 +/- 3.1 pg/ml) and current smokers (45 +/- 3.6 pg/ ml) and were increased about 1.8-fold compared with healthy smokers (24 +/- 2.6 pg/ml, p < 0.001), who had 2.2-fold higher 8Isoprostane than healthy nonsmokers (10.8 +/- 0.8 pg/ml, p < 0.05). Smoking caused an acute increase in exhaled 8Isoprostane by about 50%. Our study shows that free radical production is increased in patients with COPD and that smoking causes an acute increase in oxidative stress.