The Experts below are selected from a list of 79323 Experts worldwide ranked by ideXlab platform
Naohiko Seki - One of the best experts on this subject based on the ideXlab platform.
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The functional significance of microRNA-375 in human squamous cell carcinoma: Aberrant Expression and effects on cancer pathways
Journal of Human Genetics, 2012Co-Authors: Takashi Kinoshita, Nijiro Nohata, Toyoyuki Hanazawa, Yoshitaka Okamoto, Naohiko SekiAbstract:MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules consisting of 19–22 nucleotides that are involved in a variety of biological processes, including development, differentiation, apoptosis and cell proliferation. In cancer research, a growing body of evidence has indicated that miRNAs are Aberrantly expressed in many types of human cancers and can function either as tumor suppressors or oncogenes. Bioinformatic predictions suggest that miRNAs regulate more than 30% of protein-coding genes. Aberrant Expression of miRNAs in cancer cells causes destruction of miRNA-regulated messenger RNA networks. Therefore, the identification of miRNA-regulated cancer pathways is important for understanding the molecular mechanisms of human cancer. Searching for the Aberrant Expression of miRNAs in cancer cells is the first step in the functional analysis of miRNAs in cancer cells. Genome-wide miRNA Expression signatures can rapidly and precisely reveal Aberrant Expression of miRNA in cancers. The miRNA Expression signatures of human cancers have revealed that miR-375 is significantly downregulated in cancer cells. Our recent data on maxillary sinus, hypopharyngeal and esophageal squamous cell carcinomas have suggested that miR-375 is frequently downregulated and functions as a tumor suppressor that targets several oncogenic genes in cancer cells. In this review, we focus on several types of human squamous cell carcinoma and describe the Aberrant Expression of miRNAs and the cancer pathways they regulate in these diseases.
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The functional significance of microRNA-375 in human squamous cell carcinoma: Aberrant Expression and effects on cancer pathways
Journal of Human Genetics, 2012Co-Authors: Takashi Kinoshita, Nijiro Nohata, Toyoyuki Hanazawa, Yoshitaka Okamoto, Naohiko SekiAbstract:The functional significance of microRNA-375 in human squamous cell carcinoma: Aberrant Expression and effects on cancer pathways
Nafisa Balasinor - One of the best experts on this subject based on the ideXlab platform.
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Aberrant Expression of imprinted genes in post-implantation rat embryos.
Life Sciences, 2011Co-Authors: Neelam Kedia-mokashi, Raja C. Mugasimangalam, Mohammed Aiyaz, Srabani Mukherjee, Nafisa BalasinorAbstract:Abstract Aim Imprinted genes are known regulators of embryo growth. Studies from our laboratory have demonstrated that treatment of adult male rats with tamoxifen increased post-implantation loss at around midgestation. Expression of insulin like growth factor 2 ( Igf2 ), a paternally expressed imprinted gene was down-regulated in the resorbing embryos obtained at embryonic day 13. Hypomethylation of Igf2-H19 imprint control region was observed in the resorbing embryo sires and spermatozoa obtained from tamoxifen-treated rats thereby suggesting that errors in imprint acquisition during spermatogenesis can result in embryo loss. The present study aims at studying the Expression of other imprinted genes, besides Igf2 in the embryos sired by tamoxifen-treated males. Main methods Gene Expression profiles of resorbing versus normal embryos were assessed by microarrays. Real time quantitative RT-PCR for six imprinted genes and four genes involved in cell cycle was done to validate gene Expression data. The affected pathways and functions were identified in the resorbing embryos and effect on cell cycle was confirmed by flow cytometry. Key findings Aberrant Expression of a number of imprinted genes was observed in the resorbing embryos when compared to the normal embryos at embryonic days 11 and 13. Down-regulation of Notch signaling, Wnt signaling and cell cycle pathway was observed in the resorbing embryos. Significance The study suggests that exposure of male germ cells to tamoxifen during adulthood results in Aberrant Expression of imprinted genes and down-regulation of development associated pathways in the F 1 progeny thereby causing embryo loss.
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Aberrant Expression of imprinted genes in post-implantation rat embryos.
Life sciences, 2011Co-Authors: Neelam Kedia-mokashi, Raja C. Mugasimangalam, Mohammed Aiyaz, Srabani Mukherjee, Nafisa BalasinorAbstract:Imprinted genes are known regulators of embryo growth. Studies from our laboratory have demonstrated that treatment of adult male rats with tamoxifen increased post-implantation loss at around midgestation. Expression of insulin like growth factor 2 (Igf2), a paternally expressed imprinted gene was down-regulated in the resorbing embryos obtained at embryonic day 13. Hypomethylation of Igf2-H19 imprint control region was observed in the resorbing embryo sires and spermatozoa obtained from tamoxifen-treated rats thereby suggesting that errors in imprint acquisition during spermatogenesis can result in embryo loss. The present study aims at studying the Expression of other imprinted genes, besides Igf2 in the embryos sired by tamoxifen-treated males. Gene Expression profiles of resorbing versus normal embryos were assessed by microarrays. Real time quantitative RT-PCR for six imprinted genes and four genes involved in cell cycle was done to validate gene Expression data. The affected pathways and functions were identified in the resorbing embryos and effect on cell cycle was confirmed by flow cytometry. Aberrant Expression of a number of imprinted genes was observed in the resorbing embryos when compared to the normal embryos at embryonic days 11 and 13. Down-regulation of Notch signaling, Wnt signaling and cell cycle pathway was observed in the resorbing embryos. The study suggests that exposure of male germ cells to tamoxifen during adulthood results in Aberrant Expression of imprinted genes and down-regulation of development associated pathways in the F(1) progeny thereby causing embryo loss. Copyright © 2011 Elsevier Inc. All rights reserved.
Pavel P. Philippov - One of the best experts on this subject based on the ideXlab platform.
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Aberrant demethylation of the recoverin gene is involved in the Aberrant Expression of recoverin in cancer cells.
Experimental dermatology, 2010Co-Authors: Alexandr V. Bazhin, Charles De Smet, Marina O. Golovastova, Jan Schmidt, Pavel P. PhilippovAbstract:The Ca2+-binding protein recoverin is normally specific for the retina. Recoverin Aberrantly expressed in lung and melanoma tumors can trigger the host immune response followed by the development of a paraneoplastic neurological syndrome represented by cancer- and melanoma-associated retinopathy, respectively. The mechanisms, underlying the Aberrant Expression of recoverin in tumor cells, have remained unknown. The data obtained in this study suggest that (i) DNA methylation participates in the repression of synthesis of mRNA for recoverin in normal tissues and (ii) Aberrant hypomethylation of the recoverin gene region, overlapping the promoter up-stream of the first exon and the first exon itself, is involved in the Aberrant Expression of recoverin in tumor cells.
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Stimulation of the Aberrant Expression of a paraneoplastic antigen, recoverin, in small cell lung cancer cell lines
Lung cancer (Amsterdam Netherlands), 2004Co-Authors: Alexandr V. Bazhin, Marina S. Savchenko, Eugene V Belousov, Gabriele Jaques, Pavel P. PhilippovAbstract:Recoverin, a retina-specific Ca2+-binding protein, is one of the paraneoplastic antigens (PNAs) which are normally present in neurons, but can also be Aberrantly expressed in malignant tumors localized outside the nervous system. In this study, we have analyzed 16 small cell lung carcinoma (SCLC) and 12 non-small cell lung carcinoma cell lines and found that none of them is capable of expressing recoverin in vitro. However, two small cell lung carcinoma lines, NCI-H69 and NCI-H82, became recoverin-positive after cultivation in the presence of butyrate. Recoverin Expression in the butyrate-treated cells has been detected by immunoblotting with polyclonal (monospecific) antibodies against recoverin and confirmed by the analysis of recoverin mRNA Expression. To our knowledge, this work is the first to demonstrate stimulation of the Aberrant Expression of recoverin in cancer cell lines in vitro. This result opens the way to investigation of the mechanisms underlying the Aberrant Expression of recoverin, as well as other paraneoplastic antigens, in tumor cells.
Takashi Kinoshita - One of the best experts on this subject based on the ideXlab platform.
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The functional significance of microRNA-375 in human squamous cell carcinoma: Aberrant Expression and effects on cancer pathways
Journal of Human Genetics, 2012Co-Authors: Takashi Kinoshita, Nijiro Nohata, Toyoyuki Hanazawa, Yoshitaka Okamoto, Naohiko SekiAbstract:MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules consisting of 19–22 nucleotides that are involved in a variety of biological processes, including development, differentiation, apoptosis and cell proliferation. In cancer research, a growing body of evidence has indicated that miRNAs are Aberrantly expressed in many types of human cancers and can function either as tumor suppressors or oncogenes. Bioinformatic predictions suggest that miRNAs regulate more than 30% of protein-coding genes. Aberrant Expression of miRNAs in cancer cells causes destruction of miRNA-regulated messenger RNA networks. Therefore, the identification of miRNA-regulated cancer pathways is important for understanding the molecular mechanisms of human cancer. Searching for the Aberrant Expression of miRNAs in cancer cells is the first step in the functional analysis of miRNAs in cancer cells. Genome-wide miRNA Expression signatures can rapidly and precisely reveal Aberrant Expression of miRNA in cancers. The miRNA Expression signatures of human cancers have revealed that miR-375 is significantly downregulated in cancer cells. Our recent data on maxillary sinus, hypopharyngeal and esophageal squamous cell carcinomas have suggested that miR-375 is frequently downregulated and functions as a tumor suppressor that targets several oncogenic genes in cancer cells. In this review, we focus on several types of human squamous cell carcinoma and describe the Aberrant Expression of miRNAs and the cancer pathways they regulate in these diseases.
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The functional significance of microRNA-375 in human squamous cell carcinoma: Aberrant Expression and effects on cancer pathways
Journal of Human Genetics, 2012Co-Authors: Takashi Kinoshita, Nijiro Nohata, Toyoyuki Hanazawa, Yoshitaka Okamoto, Naohiko SekiAbstract:The functional significance of microRNA-375 in human squamous cell carcinoma: Aberrant Expression and effects on cancer pathways
Ning Zhang - One of the best experts on this subject based on the ideXlab platform.
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Hypermethylation and Aberrant Expression of secreted fizzled-related protein genes in pancreatic cancer
World journal of gastroenterology, 2008Co-Authors: Cheng-hai Zhao, Ning Zhang, Feng Gao, Shuai Lin, Xian-wei DaiAbstract:AIM: To determine the methylation status and Aberrant Expression of some secreted frizzled-related protein (SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis. METHODS: Methylation status and Expression of SFRP genes were detected by methylation-specific PCR (MSPCR) and reverse-transcription PCR (RT-PCR) respectively. RESULTS: The frequencies of methylation for SFRP genes 1, 2, 4, 5 were 70%, 48.3%, 60% and 76.7% in pancreatic cancer samples, and 21.7%, 20%, 10% and 36.7% in matched cancer adjacent normal tissue samples, respectively (χ 2 = 28.23, P < 0.0001 for SFRP gene 1; χ 2 = 10.71, P = 0.001 for SFRP gene 2; χ 2 = 32.97, P < 0.0001 for SFRP gene 4; χ 2 = 19.55, P < 0.0001 for SFRP gene 5). Expression loss of SFRP genes 1, 2, 4 and 5 was found in 65%, 40%, 55% and 71.7% of 60 pancreatic cancer samples, and 25%, 15%, 18.3% and 31.7% of matched cancer adjacent normal tissue samples, respectively (χ 2 = 19.39, P < 0.0001 for SFRP gene 1; χ 2 = 9.40, P = 0.002 for SFRP gene 2; χ 2 = 17.37, P < 0.0001 for SFRP gene 4; χ 2 = 19.22, P < 0.0001 for SFRP gene 5). SFRP gene 1 was methylated but not expressed in PC-3 and PANC-1, SFRP gene 2 was methylated but not expressed in PANC-1 and CFPAC-1, SFRP gene 4 was methylated but not expressed in PC-3, and SFRP gene 5 was methylated but not expressed in CFPAC-1. CONCLUSION: Hypermethylation and Aberrant Expression of SFRP genes are common in pancreatic cancer, which may be involved in pancreatic carcinogenesis.
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Hypermethylation and Aberrant Expression of Wnt antagonist secreted frizzled-related protein 1 in gastric cancer.
World journal of gastroenterology, 2007Co-Authors: Cheng-hai Zhao, Ning ZhangAbstract:Hypermethylation and Aberrant Expression of Wnt antagonist secreted frizzled-related protein 1 in gastric cancer
