The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Shannon L Johnson - One of the best experts on this subject based on the ideXlab platform.
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detection of abrin like and prepropulchellin like toxin genes and transcripts using whole genome sequencing and full length transcript sequencing of Abrus precatorius
Toxins, 2019Co-Authors: Blake T. Hovde, Erik R Hanschen, Yuliya A Kunde, Shawn R Starkenburg, Hajnalka E Daligault, Matthew B Johnson, Shannon L JohnsonAbstract:The sequenced genome and the leaf transcriptome of a near relative of Abrus pulchellus and Abrus precatorius was analyzed to characterize the genetic basis of toxin gene expression. From the high-quality genome assembly, a total of 26 potential coding regions were identified that contain genes with abrin-like, pulchellin-like, and agglutinin-like homology, with full-length transcripts detected in leaf tissue for 9 of the 26 coding regions. All of the toxin-like genes were identified within only five isolated regions of the genome, with each region containing 1 to 16 gene variants within each genomic region (<1 Mbp). The Abrus precatorius cultivar sequenced here contains genes which encode for proteins that are homologous to certain abrin and prepropulchellin genes previously identified, and we observed substantial diversity of genes and predicted gene products in Abrus precatorius and previously characterized toxins. This suggests diverse toxin repertoires within Abrus, potentially the results of rapid toxin evolution.
Blake T. Hovde - One of the best experts on this subject based on the ideXlab platform.
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detection of abrin like and prepropulchellin like toxin genes and transcripts using whole genome sequencing and full length transcript sequencing of Abrus precatorius
Toxins, 2019Co-Authors: Blake T. Hovde, Erik R Hanschen, Yuliya A Kunde, Shawn R Starkenburg, Hajnalka E Daligault, Matthew B Johnson, Shannon L JohnsonAbstract:The sequenced genome and the leaf transcriptome of a near relative of Abrus pulchellus and Abrus precatorius was analyzed to characterize the genetic basis of toxin gene expression. From the high-quality genome assembly, a total of 26 potential coding regions were identified that contain genes with abrin-like, pulchellin-like, and agglutinin-like homology, with full-length transcripts detected in leaf tissue for 9 of the 26 coding regions. All of the toxin-like genes were identified within only five isolated regions of the genome, with each region containing 1 to 16 gene variants within each genomic region (<1 Mbp). The Abrus precatorius cultivar sequenced here contains genes which encode for proteins that are homologous to certain abrin and prepropulchellin genes previously identified, and we observed substantial diversity of genes and predicted gene products in Abrus precatorius and previously characterized toxins. This suggests diverse toxin repertoires within Abrus, potentially the results of rapid toxin evolution.
Jichun Xing - One of the best experts on this subject based on the ideXlab platform.
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Complete mitochondrial genome of Abrus expansivus (Hemiptera: Cicadellidae: Deltocephalinae) from China
Mitochondrial DNA Part B, 2019Co-Authors: Jia-jia Wang, Jichun XingAbstract:The complete mitochondrial genome (mitogenome) of the leafhopper species Abrus expansivus Xing and Li 2014 (Hemiptera: Cicadellidae: Deltocephalinae) are sequenced and annotated. The mitochondrial ...
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Two new species of the genus Abrus Dai & Zhang, 2002... 103 Two new species of the genus Abrus Dai & Zhang, 2002
2016Co-Authors: Deltocephalinae From China, Jichun XingAbstract:Two new species of leafhoppers: Abrus damingshanensis sp. n. (from Guangxi) and A. expansivus sp. n. (from Guizhou) are described and illustrated from China. A map showing the geographic distribution of the two new species is given. Taxonomic notes on species of the genus Abrus is also provided
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Two new species of the genus Abrus Dai & Zhang, 2002 (Hemiptera, Cicadellidae, Deltocephalinae) from China.
ZooKeys, 2014Co-Authors: Jichun XingAbstract:Two new species of leafhoppers: Abrus damingshanensis sp. n. (from Guangxi) and A. expansivus sp. n. (from Guizhou) are described and illustrated from China. A map showing the geographic distribution of the two new species is given. Taxonomic notes on species of the genus Abrus is also provided.
Tapas K Maiti - One of the best experts on this subject based on the ideXlab platform.
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Abrus agglutinin targets cancer stem-like cells by eliminating self-renewal capacity accompanied with apoptosis in oral squamous cell carcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2017Co-Authors: Niharika Sinha, Tapas K Maiti, Prashanta Kumar Panda, Prajna Paramita Naik, Sujit K. BhutiaAbstract:The accumulating evidences show that Abrus agglutinin, a plant lectin, displays a broad range of anticancer activity including cancer-specific induction of apoptosis; however, the underlying molecular mechanism of Abrus agglutinin–induced oral cancer stem cell elimination remains elusive. Our data documented that Abrus agglutinin effectively downregulated the CD44+ expression with the increased CD44− population in different oral cancer cells. After 24-h Abrus agglutinin treatment, FaDu cells were quantified for orosphere formation in ultra-low attachment plates and data showed that Abrus agglutinin inhibited the number and size of orosphere in a dose-dependent manner in FaDu cells. Furthermore, Abrus agglutinin hindered the plasticity of FaDu orospheres as supported by reduced sphere formation and downregulated the self-renewal property via inhibition of Wnt-β-catenin signaling pathway. Introduction of LiCl, a glycogen synthase kinase 3β inhibitor, rescued the Abrus agglutinin–stimulated inhibition of β-c...
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Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase mediated cell death
Acta Pharmacologica Sinica, 2014Co-Authors: Subhadip Mukhopadhyay, Tapas K Maiti, Niharika Sinha, Prashanta Kumar Panda, Durgesh Nandini Das, Birendra Behera, Sujit K. BhutiaAbstract:Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death
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crabs eye Abrus precatorius seed and its immunomodulatory and antitumor properties
Nuts and Seeds in Health and Disease Prevention, 2011Co-Authors: Sujit K. Bhutia, Tapas K MaitiAbstract:Publisher Summary This chapter appraises the therapeutic properties of crabs eye seeds. While all parts of the plant are toxic, the highest concentrations of cytotoxic compounds are found in the seeds. Along with smaller concentrations of glycyrrhizin, aric acid, and N -methyltryptophan, Abrus seeds contain the toxic lectins—namely, abrin (ABR) and the relatively less toxic agglutinin commonly known as Abrus agglutinin (AGG). Abrin is a 63-kDa heterodimeric glycoprotein, whereas agglutinin is a heterotetrameric glycoprotein with a molecular weight of 134 kDa. The in vitro growth inhibition potential of Abrus lectins has been studied, and results show that the lectins inhibit the major protein synthesis pathway, which leads to cell death. The selective tumor-targeting nature of Abrus lectins ensures its place as a potential anticancer agent, and both lectins (agglutinin and abrin) inhibit the growth of tumors in experimental animals through apoptosis induction. Along with direct antitumor potential, these lectins have a functional role in tumor defense by immunomodulation. Findings indicate that Abrus lectins may be used as immunoadjuvants in aqueous solution for raising a high antibody titer with a high avidity for weak antigens, thus potentiating the systemic immune response. Abrus lectin (agglutinin and abrin)-derived peptides showed potent in vitro and in vivo antitumor and immunostimulatory properties, which may be used further as a potential source of tumor-targeting therapeutic peptides. A detailed mechanistic study of Abrus lectins mediating cancer regression in advanced malignant disease is required to evaluate their potential as alternative chemotherapeutic agents.
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efficiency of heat denatured lectins from Abrus precatorius as immunoadjuvants
Food and Agricultural Immunology, 2003Co-Authors: Sudipta Tripathi, Tapas K MaitiAbstract:Abrus agglutinin and Abrus toxin are galactose-binding lectins with strong affinity for Gal (β 1→3) GalNAc and their immunostimulatory activities are exerted through the lectin-carbohydrate interaction. In our previous work, it was demonstrated that heat denatured Abrus agglutinin in oil emulsion produces humoral immune response comparable with that of traditional complete Freund's adjuvant when used with the test antigen ovalbumin in a rat model. In the present work, we have undertaken a study to determine the immunoadjuvant activity of Abrus agglutinin (native/heat denatured) and toxin (heat denatured) in aqueous solution when co-administered with ovalbumin (OVA) as well as diphtheria toxoid (DTOX) and lysozyme (LZ) as test antigens in a mouse model system. It was found that induction of humoral immunity by native agglutinin (NA), heat denatured agglutinin (HDA) and heat denatured toxin (HDT) were comparable to that of Freund's adjuvant. The antigen specific IgG titre was much higher in comparison to ad...
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stimulation of murine macrophages by native and heat denatured lectin from Abrus precatorius
International Immunopharmacology, 2003Co-Authors: Sudipta Tripathi, Tapas K MaitiAbstract:We have evaluated the immunostimulant activity of native agglutinin (NA) and heat-denatured agglutinin (HDA) obtained from Abrus precatorius seed kernels on murine macrophages. Activated macrophages play an important role in nonspecific immunostimulation in turn by activating the other immune cells in the cascade. Significant alterations are observed in the macrophage functions both by native and heat-denatured Abrus agglutinin. Increased production of nitric oxide and hydrogen peroxide and a high phagocytic and bactericidal activity is potentiated by both NA and HDA. It is also observed that activated macrophages also released interleukin-1. These results suggest that both NA and HDA act as immunostimulants in vitro.
Yuliya A Kunde - One of the best experts on this subject based on the ideXlab platform.
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detection of abrin like and prepropulchellin like toxin genes and transcripts using whole genome sequencing and full length transcript sequencing of Abrus precatorius
Toxins, 2019Co-Authors: Blake T. Hovde, Erik R Hanschen, Yuliya A Kunde, Shawn R Starkenburg, Hajnalka E Daligault, Matthew B Johnson, Shannon L JohnsonAbstract:The sequenced genome and the leaf transcriptome of a near relative of Abrus pulchellus and Abrus precatorius was analyzed to characterize the genetic basis of toxin gene expression. From the high-quality genome assembly, a total of 26 potential coding regions were identified that contain genes with abrin-like, pulchellin-like, and agglutinin-like homology, with full-length transcripts detected in leaf tissue for 9 of the 26 coding regions. All of the toxin-like genes were identified within only five isolated regions of the genome, with each region containing 1 to 16 gene variants within each genomic region (<1 Mbp). The Abrus precatorius cultivar sequenced here contains genes which encode for proteins that are homologous to certain abrin and prepropulchellin genes previously identified, and we observed substantial diversity of genes and predicted gene products in Abrus precatorius and previously characterized toxins. This suggests diverse toxin repertoires within Abrus, potentially the results of rapid toxin evolution.
