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Vera Lucia Sarda Ribeiro - One of the best experts on this subject based on the ideXlab platform.
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Acaricidal Properties of the essential oil and precocene ii obtained from calea serrata asteraceae on the cattle tick rhipicephalus boophilus microplus acari ixodidae
Veterinary Parasitology, 2011Co-Authors: Vera Lucia Sarda Ribeiro, Jaqueline Campiol Dos Santos, Ionara Rodrigues Siqueira, Gilsane Lino Poser, Jan Schripsema, Joao Ricardo Martins, Miriam A ApelAbstract:Abstract Calea serrata Less. (Asteraceae), an endemic species of south Brazil known as “quebra-tudo”, is used in Afro-Brazilian religious rituals and in folk medicine for treating liver disorders. Phytochemical studies of the n-hexane extract of this plant demonstrated the presence of precocene II, a benzopyran derivative known for its insecticidal activity. The aim of this work was to isolate this benzopyran and determine the chemical composition of the essential oil of C. serrata and further to evaluate the Acaricidal activity of the essential oil and precocene II against the larvae of Rhipicephalus (Boophilus) microplus. The LC99.9 and LC50 values obtained with the oil, which presents precocene II and sesquiterpenes, were 3.94 μL/mL and 0.28 μL/mL, respectively. For precocene II this values were 4.25 mg/mL and 1.78 mg/mL, respectively. The results indicate a synergistic interaction between the components of the oil and precocene II.
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Acaricidal Properties of extracts from the aerial parts of hypericum polyanthemum on the cattle tick boophilus microplus
Veterinary Parasitology, 2007Co-Authors: Vera Lucia Sarda Ribeiro, Sergio Augusto De Loreto Bordignon, Eduardo Von Poser Toigo, Karla Goncalves, Gilsane Lino PoserAbstract:Laboratory tests were carried out on larvae and adults of the cattle tick Boophilus microplus to determine the toxicity of n-hexane and crude methanolic extracts of Hypericum polyanthemum (Guttiferae) using the larval immersion test (LIT) and adult immersion test (AIT). In the AIT, the effectiveness of treatment against engorged females was assessed by measuring egg production: the n-hexane extract was found to have a small effect on the egg laying at the highest concentration (19.2% of egg-laying inhibition) whilst the crude methanolic extract did not affect the egg production. For the LIT the n-hexane extracts were highly toxic to the larvae at all the concentrations (100% of mortality). The crude methanolic extract was also toxic to the larvae at higher concentrations killing 100, 96.7, 84.7 and 52.7% at the concentrations of 50, 25, 12.5, and 6.25 mg/ml, respectively, 48 h after the immersion of the acarus.
Gilsane Lino Poser - One of the best experts on this subject based on the ideXlab platform.
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Acaricidal Properties of the essential oil and precocene ii obtained from calea serrata asteraceae on the cattle tick rhipicephalus boophilus microplus acari ixodidae
Veterinary Parasitology, 2011Co-Authors: Vera Lucia Sarda Ribeiro, Jaqueline Campiol Dos Santos, Ionara Rodrigues Siqueira, Gilsane Lino Poser, Jan Schripsema, Joao Ricardo Martins, Miriam A ApelAbstract:Abstract Calea serrata Less. (Asteraceae), an endemic species of south Brazil known as “quebra-tudo”, is used in Afro-Brazilian religious rituals and in folk medicine for treating liver disorders. Phytochemical studies of the n-hexane extract of this plant demonstrated the presence of precocene II, a benzopyran derivative known for its insecticidal activity. The aim of this work was to isolate this benzopyran and determine the chemical composition of the essential oil of C. serrata and further to evaluate the Acaricidal activity of the essential oil and precocene II against the larvae of Rhipicephalus (Boophilus) microplus. The LC99.9 and LC50 values obtained with the oil, which presents precocene II and sesquiterpenes, were 3.94 μL/mL and 0.28 μL/mL, respectively. For precocene II this values were 4.25 mg/mL and 1.78 mg/mL, respectively. The results indicate a synergistic interaction between the components of the oil and precocene II.
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Acaricidal Properties of extracts from the aerial parts of hypericum polyanthemum on the cattle tick boophilus microplus
Veterinary Parasitology, 2007Co-Authors: Vera Lucia Sarda Ribeiro, Sergio Augusto De Loreto Bordignon, Eduardo Von Poser Toigo, Karla Goncalves, Gilsane Lino PoserAbstract:Laboratory tests were carried out on larvae and adults of the cattle tick Boophilus microplus to determine the toxicity of n-hexane and crude methanolic extracts of Hypericum polyanthemum (Guttiferae) using the larval immersion test (LIT) and adult immersion test (AIT). In the AIT, the effectiveness of treatment against engorged females was assessed by measuring egg production: the n-hexane extract was found to have a small effect on the egg laying at the highest concentration (19.2% of egg-laying inhibition) whilst the crude methanolic extract did not affect the egg production. For the LIT the n-hexane extracts were highly toxic to the larvae at all the concentrations (100% of mortality). The crude methanolic extract was also toxic to the larvae at higher concentrations killing 100, 96.7, 84.7 and 52.7% at the concentrations of 50, 25, 12.5, and 6.25 mg/ml, respectively, 48 h after the immersion of the acarus.
Miriam A Apel - One of the best experts on this subject based on the ideXlab platform.
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Acaricidal Properties of the essential oil and precocene ii obtained from calea serrata asteraceae on the cattle tick rhipicephalus boophilus microplus acari ixodidae
Veterinary Parasitology, 2011Co-Authors: Vera Lucia Sarda Ribeiro, Jaqueline Campiol Dos Santos, Ionara Rodrigues Siqueira, Gilsane Lino Poser, Jan Schripsema, Joao Ricardo Martins, Miriam A ApelAbstract:Abstract Calea serrata Less. (Asteraceae), an endemic species of south Brazil known as “quebra-tudo”, is used in Afro-Brazilian religious rituals and in folk medicine for treating liver disorders. Phytochemical studies of the n-hexane extract of this plant demonstrated the presence of precocene II, a benzopyran derivative known for its insecticidal activity. The aim of this work was to isolate this benzopyran and determine the chemical composition of the essential oil of C. serrata and further to evaluate the Acaricidal activity of the essential oil and precocene II against the larvae of Rhipicephalus (Boophilus) microplus. The LC99.9 and LC50 values obtained with the oil, which presents precocene II and sesquiterpenes, were 3.94 μL/mL and 0.28 μL/mL, respectively. For precocene II this values were 4.25 mg/mL and 1.78 mg/mL, respectively. The results indicate a synergistic interaction between the components of the oil and precocene II.
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Acaricidal Properties of the essential oil from hesperozygis ringens lamiaceae on the cattle tick riphicephalus boophilus microplus
Bioresource Technology, 2010Co-Authors: Vera Ribeiro, Jaqueline Campiol Dos Santos, Miriam A Apel, Sergio Augusto De Loreto Bordignon, Amelia T Henriques, Gilsane Lino Von PoserAbstract:Abstract Hesperozygis ringens (Benth.) Epling (Lamiaceae) is a strongly aromatic plant employed popularly for its antiparasitic Properties. The leaves afforded 4% of essential oil constituted mainly by pulegone (86%). Laboratory tests were carried out to determine the toxicity of the essential oil species on engorged females and larvae of the cattle tick Riphicephalus ( Boophilus ) microplus using the adult immersion test (AIT) and the larval immersion test (LIT). It was observed that the essential oil at the concentration of 50 μL/mL and 25 μL/mL inhibited the egg laying significatively in relation to the controls and the eggs from these treated females were affected by the oil; the hatching was inhibited in 95% and 30%, respectively. In the LIT it was verified that the LC 99.9 , LC 50 and LC 1 was 0.541 μL/mL, 0.260 μL/mL and 0.015 μL/mL, respectively. Pulegone, isolated from the oil, showed similar effect on the females and on the larvae, indicating that it is responsible for the oil activity.
Dorothee Stanneck - One of the best experts on this subject based on the ideXlab platform.
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efficacy of an imidacloprid flumethrin collar against fleas ticks mites and lice on dogs
Parasites & Vectors, 2012Co-Authors: Dorothee Stanneck, Eva M Kruedewagen, W L Davis, Klemens KriegerAbstract:Background: The studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. Efficacy was evaluated against the flea Ctenocephalides felis felis, the ticks Rhipicephalus sanguineus, Ixodes ricinus, Ixodes scapularis, Dermacentor reticulatus and Dermacentor variabilis, the mite Sarcoptes scabiei and the biting louse Trichodectes canis. Methods: Groups of collar-treated dogs (n=7–10) were infested with fleas and/or ticks at monthly intervals at least, over a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after each re-infestation. Efficacy against ticks was evaluated at 48 h (Acaricidal), 6 h (repellent) and 48 h (sustained) after infestation. The effect of regular shampooing or immersion in water on the efficacy of the collars was also tested. Efficacy against flea larvae was assessed by incubating blanket samples after dog contact with viable flea eggs. Effectiveness against lice and mites was evaluated after treatment of naturally infested animals. With the exception of the mites, efficacy was calculated by comparison with untreated negative control groups. Results: Efficacy against fleas (24 h) generally exceeded 95%, and against flea larvae it exceeded 99% for 8 months. Sustained Acaricidal (48 h) efficacy, covering a period of 8 months was 100% against I. ricinus, starting 2 days after treatment (in vivo), and 100% against I. scapularis (in vitro), above 97% against R. sanguineus, generally above 97% against D. reticulatus and above 90% for D. variabilis. Repellent (6 h) efficacy 2 days after treatment and continuing for 8 months was consistently 100% against I. ricinus, and above 90% against R. sanguineus. Regular shampooing affected efficacy against fleas and ticks to a lesser extent than regular immersion in water. The collars eliminated Trichodectes canis within 2 days and Sarcoptes scabiei within 3 months. Conclusion: The rapid insecticidal and Acaricidal Properties of the medicated collars against newly-acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been clearly shown. Consequently they have the potential to prevent the transmission of vector-borne diseases and other conditions directly associated with infestation throughout an entire season of parasite abundance.
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Efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs
Parasites & Vectors, 2012Co-Authors: Dorothee Stanneck, Eva M Kruedewagen, Wendell Davis, Klemens J KriegerAbstract:Background The studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. Efficacy was evaluated against the flea Ctenocephalides felis felis , the ticks Rhipicephalus sanguineus , Ixodes ricinus , Ixodes scapularis , Dermacentor reticulatus and Dermacentor variabilis, the mite Sarcoptes scabiei and the biting louse Trichodectes canis . Methods Groups of collar-treated dogs (n = 7–10) were infested with fleas and/or ticks at monthly intervals at least, over a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after each re-infestation. Efficacy against ticks was evaluated at 48 h (Acaricidal), 6 h (repellent) and 48 h (sustained) after infestation. The effect of regular shampooing or immersion in water on the efficacy of the collars was also tested. Efficacy against flea larvae was assessed by incubating blanket samples after dog contact with viable flea eggs. Effectiveness against lice and mites was evaluated after treatment of naturally infested animals. With the exception of the mites, efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h) generally exceeded 95%, and against flea larvae it exceeded 99% for 8 months. Sustained Acaricidal (48 h) efficacy, covering a period of 8 months was 100% against I. ricinus , starting 2 days after treatment ( in vivo ), and 100% against I. scapularis ( in vitro ), above 97% against R. sanguineus, generally above 97% against D. reticulatus and above 90% for D. variabilis . Repellent (6 h) efficacy 2 days after treatment and continuing for 8 months was consistently 100% against I. ricinus , and above 90% against R. sanguineus . Regular shampooing affected efficacy against fleas and ticks to a lesser extent than regular immersion in water. The collars eliminated Trichodectes canis within 2 days and Sarcoptes scabiei within 3 months. Conclusion The rapid insecticidal and Acaricidal Properties of the medicated collars against newly-acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been clearly shown. Consequently they have the potential to prevent the transmission of vector-borne diseases and other conditions directly associated with infestation throughout an entire season of parasite abundance.
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Efficacy of an imidacloprid/flumethrin collar against fleas and ticks on cats
Parasites & Vectors, 2012Co-Authors: Dorothee Stanneck, Eva M Kruedewagen, Wendell Davis, Ivan Gerard Horak, Josephus J Fourie, Klemens J KriegerAbstract:Background The objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w) and 4.5% flumethrin (w/w) incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. Efficacy was evaluated against the flea Ctenocephalides felis felis , and the ticks Ixodes ricinus , Amblyomma americanum and Rhipicephalus turanicus . The number of studies was so large that only a general overview can be presented in this abstract. Methods Preventive efficacy was evaluated by infesting groups of cats (n = 8-10) with C. felis felis and/or I. ricinus, A. americanum or R. turanicus at monthly intervals at least, for a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after infestation, and against ticks at 6 h (repellent) or 48 h (Acaricidal) after infestation. Efficacy against flea larvae was evaluated over a period of 8 months by incubating viable flea eggs on blanket samples after cat contact. In all cases efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h) generally exceeded 95% until study termination. In vitro efficacy against flea larvae exceeded 92% until Day 90 and then declined to 67% at the conclusion of the study on Day 230. Sustained Acaricidal (48 h) efficacy over a period of eight months was consistently 100% against I. ricinus from Day 2 after treatment, 100% against A. americanum , except for 98.5% and 97.7% at two time-points, and between 94% and 100% against R. turanicus . From Day 2 until 8 months after treatment the repellent (6 h), efficacy was consistently 100% against I. ricinus, and between 54.8% and 85.4% against R. turanicus . Conclusion The rapid insecticidal and Acaricidal Properties of the medicated collars against newly- acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been clearly demonstrated. Taking into account the seasonality of fleas and ticks, the collars have the potential to prevent the transmission of vector-borne diseases and other conditions directly associated with infestation throughout the season of parasite abundance.
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efficacy of an imidacloprid flumethrin collar against fleas and ticks on cats
Parasites & Vectors, 2012Co-Authors: Dorothee Stanneck, Eva M Kruedewagen, Ivan Gerard Horak, Josephus J Fourie, W L Davis, Klemens KriegerAbstract:Background: The objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w) and 4.5% flumethrin (w/w) incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. Efficacy was evaluated against the flea Ctenocephalides felis felis, and the ticks Ixodes ricinus, Amblyomma americanum and Rhipicephalus turanicus. The number of studies was so large that only a general overview can be presented in this abstract. Methods: Preventive efficacy was evaluated by infesting groups of cats (n=8-10) with C. felis felis and/or I. ricinus, A. americanum or R. turanicus at monthly intervals at least, for a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after infestation, and against ticks at 6 h (repellent) or 48 h (Acaricidal) after infestation. Efficacy against flea larvae was evaluated over a period of 8 months by incubating viable flea eggs on blanket samples after cat contact. In all cases efficacy was calculated by comparison with untreated negative control groups. Results: Efficacy against fleas (24 h) generally exceeded 95% until study termination. In vitro efficacy against flea larvae exceeded 92% until Day 90 and then declined to 67% at the conclusion of the study on Day 230. Sustained Acaricidal (48 h) efficacy over a period of eight months was consistently 100% against I. ricinus from Day 2 after treatment, 100% against A. americanum, except for 98.5% and 97.7% at two time-points, and between 94% and 100% against R. turanicus. From Day 2 until 8 months after treatment the repellent (6 h), efficacy was consistently 100% against I. ricinus, and between 54.8% and 85.4% against R. turanicus. Conclusion: The rapid insecticidal and Acaricidal Properties of the medicated collars against newly- acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been clearly demonstrated. Taking into account the seasonality of fleas and ticks, the collars have the potential to prevent the transmission of vector-borne diseases and other conditions directly associated with infestation throughout the season of parasite abundance.
Klemens J Krieger - One of the best experts on this subject based on the ideXlab platform.
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Efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs
Parasites & Vectors, 2012Co-Authors: Dorothee Stanneck, Eva M Kruedewagen, Wendell Davis, Klemens J KriegerAbstract:Background The studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. Efficacy was evaluated against the flea Ctenocephalides felis felis , the ticks Rhipicephalus sanguineus , Ixodes ricinus , Ixodes scapularis , Dermacentor reticulatus and Dermacentor variabilis, the mite Sarcoptes scabiei and the biting louse Trichodectes canis . Methods Groups of collar-treated dogs (n = 7–10) were infested with fleas and/or ticks at monthly intervals at least, over a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after each re-infestation. Efficacy against ticks was evaluated at 48 h (Acaricidal), 6 h (repellent) and 48 h (sustained) after infestation. The effect of regular shampooing or immersion in water on the efficacy of the collars was also tested. Efficacy against flea larvae was assessed by incubating blanket samples after dog contact with viable flea eggs. Effectiveness against lice and mites was evaluated after treatment of naturally infested animals. With the exception of the mites, efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h) generally exceeded 95%, and against flea larvae it exceeded 99% for 8 months. Sustained Acaricidal (48 h) efficacy, covering a period of 8 months was 100% against I. ricinus , starting 2 days after treatment ( in vivo ), and 100% against I. scapularis ( in vitro ), above 97% against R. sanguineus, generally above 97% against D. reticulatus and above 90% for D. variabilis . Repellent (6 h) efficacy 2 days after treatment and continuing for 8 months was consistently 100% against I. ricinus , and above 90% against R. sanguineus . Regular shampooing affected efficacy against fleas and ticks to a lesser extent than regular immersion in water. The collars eliminated Trichodectes canis within 2 days and Sarcoptes scabiei within 3 months. Conclusion The rapid insecticidal and Acaricidal Properties of the medicated collars against newly-acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been clearly shown. Consequently they have the potential to prevent the transmission of vector-borne diseases and other conditions directly associated with infestation throughout an entire season of parasite abundance.
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Efficacy of an imidacloprid/flumethrin collar against fleas and ticks on cats
Parasites & Vectors, 2012Co-Authors: Dorothee Stanneck, Eva M Kruedewagen, Wendell Davis, Ivan Gerard Horak, Josephus J Fourie, Klemens J KriegerAbstract:Background The objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w) and 4.5% flumethrin (w/w) incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. Efficacy was evaluated against the flea Ctenocephalides felis felis , and the ticks Ixodes ricinus , Amblyomma americanum and Rhipicephalus turanicus . The number of studies was so large that only a general overview can be presented in this abstract. Methods Preventive efficacy was evaluated by infesting groups of cats (n = 8-10) with C. felis felis and/or I. ricinus, A. americanum or R. turanicus at monthly intervals at least, for a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after infestation, and against ticks at 6 h (repellent) or 48 h (Acaricidal) after infestation. Efficacy against flea larvae was evaluated over a period of 8 months by incubating viable flea eggs on blanket samples after cat contact. In all cases efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h) generally exceeded 95% until study termination. In vitro efficacy against flea larvae exceeded 92% until Day 90 and then declined to 67% at the conclusion of the study on Day 230. Sustained Acaricidal (48 h) efficacy over a period of eight months was consistently 100% against I. ricinus from Day 2 after treatment, 100% against A. americanum , except for 98.5% and 97.7% at two time-points, and between 94% and 100% against R. turanicus . From Day 2 until 8 months after treatment the repellent (6 h), efficacy was consistently 100% against I. ricinus, and between 54.8% and 85.4% against R. turanicus . Conclusion The rapid insecticidal and Acaricidal Properties of the medicated collars against newly- acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been clearly demonstrated. Taking into account the seasonality of fleas and ticks, the collars have the potential to prevent the transmission of vector-borne diseases and other conditions directly associated with infestation throughout the season of parasite abundance.
