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Pingjin Gao – One of the best experts on this subject based on the ideXlab platform.

  • Beta3 adrenergic receptor is involved in vascular injury in deoxycorticosterone AcetateSalt hypertensive mice.
    FEBS letters, 2016
    Co-Authors: Li-juan Sheng, Cheng-chao Ruan, Dong-rui Chen, Ling-ran Kong, Dingliang Zhu, Pingjin Gao

    Abstract:

    Beta3 adrenergic receptor (ADRB3) mediates vessel relaxation in the endothelium while it modulates lipolysis in the adipose tissue. However, the function and regulation mechanism of ADRB3 in the perivascular adipose tissue (PVAT), especially in hypertension, is still unclear. We show that ADRB3 protein is upregulated in the PVAT of deoxycorticosterone AcetateSalt (DOCA-Salt) hypertensive mice, with the characteristics of PVAT browning and increased uncoupling protein 1 (UCP1) expression. Inhibition of ADRB3 with selective antagonist SR59230A caused serious vascular injury in vivo, even though UCP1 expression was downregulated. ADRB3 protein was regulated by let-7b, which was decreased in the PVAT of the DOCA-Salt group. These data reveal that ADRB3 in PVAT contributes to vascular function in the progression of hypertension.

  • perivascular adipose tissue derived complement 3 is required for adventitial fibroblast functions and adventitial remodeling in deoxycorticosterone Acetate Salt hypertensive rats
    Arteriosclerosis Thrombosis and Vascular Biology, 2010
    Co-Authors: Cheng-chao Ruan, Qizhi Chen, Jing Chen, Dingliang Zhu, Shujie Guo, Pingjin Gao

    Abstract:

    Objective— To examine the role of perivascular adipose tissue (PVAT)–derived factors in the regulation of adventitial fibroblast (AF) function in vitro and in vivo.

    Methods and Results— PVAT is an active component of blood vessels. Bioactive substances released from PVAT play regulatory roles in vascular function. However, their effects on vascular AFs remain unclear. PVAT-conditioned medium stimulated AF migration using a transwell technique, and differentiation was evaluated by α-smooth muscle–actin induction. We identified the secretome of PVAT by liquid chromatography–tandem mass spectrometry. One of the major secretory proteins in PVAT is complement 3 (C3). The C3 antagonist and neutralizing antibody attenuated PVAT-conditioned medium–induced AF migration and differentiation. Similar to PVAT-conditioned medium, C3 recombinant protein stimulated AF migration and differentiation. We demonstrated that the effects of PVAT-derived C3 were mediated by the c-Jun N-terminal kinase pathway. Moreover, we found morphological changes in perivascular adipocytes and increased expression of C3 in PVAT that was tightly associated with adventitial thickening and myofibroblast clustering around PVAT in deoxycorticosterone AcetateSalt hypertensive rats.

    Conclusion— PVAT-derived C3 stimulated AF migration and differentiation via the c-Jun N-terminal kinase pathway. PVAT-derived C3 may contribute to adventitial remodeling in a deoxycorticosterone AcetateSalt hypertensive model.

  • Perivascular Adipose Tissue–Derived Complement 3 Is Required for Adventitial Fibroblast Functions and Adventitial Remodeling in Deoxycorticosterone AcetateSalt Hypertensive Rats
    Arteriosclerosis thrombosis and vascular biology, 2010
    Co-Authors: Cheng-chao Ruan, Qizhi Chen, Jing Chen, Dingliang Zhu, Shujie Guo, Pingjin Gao

    Abstract:

    Objective— To examine the role of perivascular adipose tissue (PVAT)–derived factors in the regulation of adventitial fibroblast (AF) function in vitro and in vivo.

    Methods and Results— PVAT is an active component of blood vessels. Bioactive substances released from PVAT play regulatory roles in vascular function. However, their effects on vascular AFs remain unclear. PVAT-conditioned medium stimulated AF migration using a transwell technique, and differentiation was evaluated by α-smooth muscle–actin induction. We identified the secretome of PVAT by liquid chromatography–tandem mass spectrometry. One of the major secretory proteins in PVAT is complement 3 (C3). The C3 antagonist and neutralizing antibody attenuated PVAT-conditioned medium–induced AF migration and differentiation. Similar to PVAT-conditioned medium, C3 recombinant protein stimulated AF migration and differentiation. We demonstrated that the effects of PVAT-derived C3 were mediated by the c-Jun N-terminal kinase pathway. Moreover, we found morphological changes in perivascular adipocytes and increased expression of C3 in PVAT that was tightly associated with adventitial thickening and myofibroblast clustering around PVAT in deoxycorticosterone AcetateSalt hypertensive rats.

    Conclusion— PVAT-derived C3 stimulated AF migration and differentiation via the c-Jun N-terminal kinase pathway. PVAT-derived C3 may contribute to adventitial remodeling in a deoxycorticosterone AcetateSalt hypertensive model.

Yi-ling Chen – One of the best experts on this subject based on the ideXlab platform.

  • Renal Damages in Deoxycorticosterone AcetateSalt Hypertensive Rats: Assessment with Diffusion Tensor Imaging and T2-mapping
    Molecular Imaging and Biology, 2020
    Co-Authors: Sheung-fat Ko, Yen-yi Zhen, Chi-chih Hung, Chung-cheng Huang, Shu-hang Ng, Yi-ling Chen

    Abstract:

    Purpose This study aimed to investigate the feasibility of diffusion tensor imaging (DTI) and T2-mapping to assess temporal renal damage in deoxycorticosterone AcetateSalt (DOCA-Salt) hypertensive rats and compare the results with histopathologic and immunohistochemical findings. Procedures After baseline renal magnetic resonance imaging (MRI), 24 out of 30 uninephrectomized Sprague-Dawley rats with DOCA-Salt-induced hypertension were divided equally into four groups. Group 1 had renal MRI at weeks 2, 4, 6, and 8, and groups 2, 3, and 4 had MRI at weeks 2, 4, and 6, respectively. The remaining 6 rats were used as sham controls. The renal cortex and outer and inner stripes of the outer medulla were examined over time using fractional anisotropy (FA), apparent diffusion coefficient (ADC), and T2-mapping, and the results were compared with baseline values. The degree of glomerular and tubular injury, endothelial cell thickening, hyaline arteriolosclerosis, macrophage infiltration, microcyst formation, and fibrosis in different zones at different time points in the DOCA-Salt rats were compared with controls. Results Compared with baseline values, DOCA-Salt rats demonstrated a significant decrease in renal cortical FA from week 4 to week 8 (0.244 ± 0.015 vs 0.172 ± 0.014–0.150 ± 0.016, P  = 0.018–0.002), corresponding to significantly more glomerular damage, arteriolosclerosis, macrophage infiltration, and fibrosis. The DOCA-Salt rats had significantly increased cortical ADC and T2 values at weeks 6 and 8 (1.778 ± 0.051 × 10^−3 mm^2/s vs 1.872 ± 0.058–1.917 ± 0.066 × 10^−3 mm^2/s; 93.7 ± 4.9 ms vs 98.0 ± 2.9–100.7 ± 4.0 ms, respectively, all P  

  • renal damages in deoxycorticosterone Acetate Salt hypertensive rats assessment with diffusion tensor imaging and t2 mapping
    Molecular Imaging and Biology, 2020
    Co-Authors: Honkan Yip, Yi-ling Chen, Yen-yi Zhen, Chi-chih Hung, Chung-cheng Huang, Chenchang Lee, Juiwei Lin

    Abstract:

    PURPOSE This study aimed to investigate the feasibility of diffusion tensor imaging (DTI) and T2-mapping to assess temporal renal damage in deoxycorticosterone AcetateSalt (DOCA-Salt) hypertensive rats and compare the results with histopathologic and immunohistochemical findings. PROCEDURES After baseline renal magnetic resonance imaging (MRI), 24 out of 30 uninephrectomized Sprague-Dawley rats with DOCA-Salt-induced hypertension were divided equally into four groups. Group 1 had renal MRI at weeks 2, 4, 6, and 8, and groups 2, 3, and 4 had MRI at weeks 2, 4, and 6, respectively. The remaining 6 rats were used as sham controls. The renal cortex and outer and inner stripes of the outer medulla were examined over time using fractional anisotropy (FA), apparent diffusion coefficient (ADC), and T2-mapping, and the results were compared with baseline values. The degree of glomerular and tubular injury, endothelial cell thickening, hyaline arteriolosclerosis, macrophage infiltration, microcyst formation, and fibrosis in different zones at different time points in the DOCA-Salt rats were compared with controls. RESULTS Compared with baseline values, DOCA-Salt rats demonstrated a significant decrease in renal cortical FA from week 4 to week 8 (0.244 ± 0.015 vs 0.172 ± 0.014-0.150 ± 0.016, P = 0.018-0.002), corresponding to significantly more glomerular damage, arteriolosclerosis, macrophage infiltration, and fibrosis. The DOCA-Salt rats had significantly increased cortical ADC and T2 values at weeks 6 and 8 (1.778 ± 0.051 × 10-3 mm2/s vs 1.872 ± 0.058-1.917 ± 0.066 × 10-3 mm2/s; 93.7 ± 4.9 ms vs 98.0 ± 2.9-100.7 ± 4.0 ms, respectively, all P < 0.05), consistent with excessively fluid-filled microcysts (aquaporin-2+). Despite DOCA-Salt rats harbored markedly increased fibrosis in outer and inner stripes of the outer medulla at weeks 6 and 8, only nonsignificant decreases in FA were observed in comparison with the controls suggesting that only limited microstructural changes were present. CONCLUSIONS Renal cortical FA is useful for the early detection and monitoring of renal damage in DOCA-Salt hypertensive rats.

  • Renal Damages in Deoxycorticosterone AcetateSalt Hypertensive Rats: Assessment with Diffusion Tensor Imaging and T2-mapping.
    Molecular Imaging and Biology, 2019
    Co-Authors: Sheung-fat Ko, Yen-yi Zhen, Chi-chih Hung, Chung-cheng Huang, Shu-hang Ng, Yi-ling Chen

    Abstract:

    PURPOSE: This study aimed to investigate the feasibility of diffusion tensor imaging (DTI) and T2-mapping to assess temporal renal damage in deoxycorticosterone AcetateSalt (DOCA-Salt) hypertensive rats and compare the results with histopathologic and immunohistochemical findings. PROCEDURES: After baseline renal magnetic resonance imaging (MRI), 24 out of 30 uninephrectomized Sprague-Dawley rats with DOCA-Salt-induced hypertension were divided equally into four groups. Group 1 had renal MRI at weeks 2, 4, 6, and 8, and groups 2, 3, and 4 had MRI at weeks 2, 4, and 6, respectively. The remaining 6 rats were used as sham controls. The renal cortex and outer and inner stripes of the outer medulla were examined over time using fractional anisotropy (FA), apparent diffusion coefficient (ADC), and T2-mapping, and the results were compared with baseline values. The degree of glomerular and tubular injury, endothelial cell thickening, hyaline arteriolosclerosis, macrophage infiltration, microcyst formation, and fibrosis in different zones at different time points in the DOCA-Salt rats were compared with controls. RESULTS: Compared with baseline values, DOCA-Salt rats demonstrated a significant decrease in renal cortical FA from week 4 to week 8 (0.244 ± 0.015 vs 0.172 ± 0.014-0.150 ± 0.016, P = 0.018-0.002), corresponding to significantly more glomerular damage, arteriolosclerosis, macrophage infiltration, and fibrosis. The DOCA-Salt rats had significantly increased cortical ADC and T2 values at weeks 6 and 8 (1.778 ± 0.051 × 10-3 mm2/s vs 1.872 ± 0.058-1.917 ± 0.066 × 10-3 mm2/s; 93.7 ± 4.9 ms vs 98.0 ± 2.9-100.7 ± 4.0 ms, respectively, all P 

Cheng-chao Ruan – One of the best experts on this subject based on the ideXlab platform.

  • Beta3 adrenergic receptor is involved in vascular injury in deoxycorticosterone AcetateSalt hypertensive mice.
    FEBS letters, 2016
    Co-Authors: Li-juan Sheng, Cheng-chao Ruan, Dong-rui Chen, Ling-ran Kong, Dingliang Zhu, Pingjin Gao

    Abstract:

    Beta3 adrenergic receptor (ADRB3) mediates vessel relaxation in the endothelium while it modulates lipolysis in the adipose tissue. However, the function and regulation mechanism of ADRB3 in the perivascular adipose tissue (PVAT), especially in hypertension, is still unclear. We show that ADRB3 protein is upregulated in the PVAT of deoxycorticosterone AcetateSalt (DOCA-Salt) hypertensive mice, with the characteristics of PVAT browning and increased uncoupling protein 1 (UCP1) expression. Inhibition of ADRB3 with selective antagonist SR59230A caused serious vascular injury in vivo, even though UCP1 expression was downregulated. ADRB3 protein was regulated by let-7b, which was decreased in the PVAT of the DOCA-Salt group. These data reveal that ADRB3 in PVAT contributes to vascular function in the progression of hypertension.

  • perivascular adipose tissue derived complement 3 is required for adventitial fibroblast functions and adventitial remodeling in deoxycorticosterone Acetate Salt hypertensive rats
    Arteriosclerosis Thrombosis and Vascular Biology, 2010
    Co-Authors: Cheng-chao Ruan, Qizhi Chen, Jing Chen, Dingliang Zhu, Shujie Guo, Pingjin Gao

    Abstract:

    Objective— To examine the role of perivascular adipose tissue (PVAT)–derived factors in the regulation of adventitial fibroblast (AF) function in vitro and in vivo.

    Methods and Results— PVAT is an active component of blood vessels. Bioactive substances released from PVAT play regulatory roles in vascular function. However, their effects on vascular AFs remain unclear. PVAT-conditioned medium stimulated AF migration using a transwell technique, and differentiation was evaluated by α-smooth muscle–actin induction. We identified the secretome of PVAT by liquid chromatography–tandem mass spectrometry. One of the major secretory proteins in PVAT is complement 3 (C3). The C3 antagonist and neutralizing antibody attenuated PVAT-conditioned medium–induced AF migration and differentiation. Similar to PVAT-conditioned medium, C3 recombinant protein stimulated AF migration and differentiation. We demonstrated that the effects of PVAT-derived C3 were mediated by the c-Jun N-terminal kinase pathway. Moreover, we found morphological changes in perivascular adipocytes and increased expression of C3 in PVAT that was tightly associated with adventitial thickening and myofibroblast clustering around PVAT in deoxycorticosterone AcetateSalt hypertensive rats.

    Conclusion— PVAT-derived C3 stimulated AF migration and differentiation via the c-Jun N-terminal kinase pathway. PVAT-derived C3 may contribute to adventitial remodeling in a deoxycorticosterone AcetateSalt hypertensive model.

  • Perivascular Adipose Tissue–Derived Complement 3 Is Required for Adventitial Fibroblast Functions and Adventitial Remodeling in Deoxycorticosterone AcetateSalt Hypertensive Rats
    Arteriosclerosis thrombosis and vascular biology, 2010
    Co-Authors: Cheng-chao Ruan, Qizhi Chen, Jing Chen, Dingliang Zhu, Shujie Guo, Pingjin Gao

    Abstract:

    Objective— To examine the role of perivascular adipose tissue (PVAT)–derived factors in the regulation of adventitial fibroblast (AF) function in vitro and in vivo.

    Methods and Results— PVAT is an active component of blood vessels. Bioactive substances released from PVAT play regulatory roles in vascular function. However, their effects on vascular AFs remain unclear. PVAT-conditioned medium stimulated AF migration using a transwell technique, and differentiation was evaluated by α-smooth muscle–actin induction. We identified the secretome of PVAT by liquid chromatography–tandem mass spectrometry. One of the major secretory proteins in PVAT is complement 3 (C3). The C3 antagonist and neutralizing antibody attenuated PVAT-conditioned medium–induced AF migration and differentiation. Similar to PVAT-conditioned medium, C3 recombinant protein stimulated AF migration and differentiation. We demonstrated that the effects of PVAT-derived C3 were mediated by the c-Jun N-terminal kinase pathway. Moreover, we found morphological changes in perivascular adipocytes and increased expression of C3 in PVAT that was tightly associated with adventitial thickening and myofibroblast clustering around PVAT in deoxycorticosterone AcetateSalt hypertensive rats.

    Conclusion— PVAT-derived C3 stimulated AF migration and differentiation via the c-Jun N-terminal kinase pathway. PVAT-derived C3 may contribute to adventitial remodeling in a deoxycorticosterone AcetateSalt hypertensive model.