Acetyl-CoA Acetyltransferase

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Hitoshi Arita - One of the best experts on this subject based on the ideXlab platform.

  • synergistic effect of throm ane a2 and n formylmethionylleucylphenylalanine on platelet activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita
    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

  • Synergistic effect of throm☐ane A2 and N-formylmethionylleucylphenylalanine on platelet-activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita
    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

Junji Kishino - One of the best experts on this subject based on the ideXlab platform.

  • synergistic effect of throm ane a2 and n formylmethionylleucylphenylalanine on platelet activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita
    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

  • Synergistic effect of throm☐ane A2 and N-formylmethionylleucylphenylalanine on platelet-activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita
    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

Keizo Waku - One of the best experts on this subject based on the ideXlab platform.

  • Transient activation of 1-O-alkyl-sn-glycero-3-phosphocholine: Acetyl-CoA Acetyltransferase during the incubation of macrophages
    Lipids, 1991
    Co-Authors: Takayuki Sugiura, Teruo Fukuda, Neng-neng Cheng, Keizo Waku
    Abstract:

    The activity of the platelet-activating factor (PAF)-synthesizing enzyme, 1- O -alkyl- sn -glycero-3-phosphocholine (lysoPAF):Acetyl-CoA Acetyltransferase (EC 2.3.1.67) in alveolar macrophage lysate was found to be elevated after warming the cells to 37°C. Such an increase in enzyme activity was detectable only when intact cells were warmed. The stimulation was transient, reaching a peak at 2 min, and then gradually decreased to the control level. We could not find increased PAF formation in warmed cells which had increased Acetyltransferase activity, even though substantial amounts of lysoPAF were shown to be present within cells. In contrast, considerable amounts of PAF were formed after treatments of the cells with exogenous lysoPAF. These results suggest that the activation of Acetyltransferase is not sufficient to induce PAF formation and that the increased availability of substrates, especially lysoPAF, in the cells is indispensable for triggering PAF biosynthesis in this type of cells.

Hideaki Yukawa - One of the best experts on this subject based on the ideXlab platform.

  • Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli
    Applied Microbiology and Biotechnology, 2008
    Co-Authors: Masayuki Inui, Masako Suda, Sakurako Kimura, Kaori Yasuda, Hiroshi Toda, Shogo Yamamoto, Shohei Okino, Nobuaki Suzuki, Hiroaki Suzuki, Hideaki Yukawa
    Abstract:

    A recombinant butanol pathway composed of Clostridium acetobutylicum ATCC 824 genes, thiL, hbd, crt, bcd-etfB-etfA , and adhe1 (or adhe ) coding for Acetyl-CoA Acetyltransferase (THL), β-hydroxybutyryl-CoA dehydrogenase (HBD), 3-hydroxybutyryl-CoA dehydratase (CRT), butyryl-CoA dehydrogenase (BCD), butyraldehyde dehydrogenase (BYDH), and butanol dehydrogenase (BDH), under the tac promoter control was constructed and was introduced into Escherichia coli . The functional expression of these six enzymes was proved by demonstrating the corresponding enzyme activities using spectrophotometric, high performance liquid chromatography and gas chromatography analyses. The BCD activity, which was not detected in E. coli previously, was shown in the present study by performing the procedure from cell extract preparation to activity measurement under anaerobic condition. Moreover, the etfA and etfB co-expression was found to be essential for the BCD activity. In the case of BYDH activity, the adhe gene product was shown to have higher specificity towards butyryl-CoA compared to the adhe1 product. Butanol production from glucose was achieved by the highly concentrated cells of the butanologenic E. coli strains, BUT1 with adhe1 and BUT2 with adhe , under anaerobic condition, and the BUT1 and BUT2 strains were shown to produce 4 and 16-mM butanol with 6- and 1-mM butyrate as a byproduct, respectively. This study reports the novel butanol production by an aerobically pregrown microorganism possessing the genes of a strict anaerobe, Clostridium acetobutylicum .

Kohji Hanasaki - One of the best experts on this subject based on the ideXlab platform.

  • synergistic effect of throm ane a2 and n formylmethionylleucylphenylalanine on platelet activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita
    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

  • Synergistic effect of throm☐ane A2 and N-formylmethionylleucylphenylalanine on platelet-activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita
    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.