Acetyl-CoA Acetyltransferase - Explore the Science & Experts | ideXlab

Scan Science and Technology

Contact Leading Edge Experts & Companies

Acetyl-CoA Acetyltransferase

The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform

Hitoshi Arita – 1st expert on this subject based on the ideXlab platform

  • synergistic effect of throm ane a2 and n formylmethionylleucylphenylalanine on platelet activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita

    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

  • Synergistic effect of throm☐ane A2 and N-formylmethionylleucylphenylalanine on platelet-activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita

    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

Junji Kishino – 2nd expert on this subject based on the ideXlab platform

  • synergistic effect of throm ane a2 and n formylmethionylleucylphenylalanine on platelet activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita

    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

  • Synergistic effect of throm☐ane A2 and N-formylmethionylleucylphenylalanine on platelet-activating factor synthesis in human polymorphonuclear neutrophils
    Biochimica et Biophysica Acta, 1991
    Co-Authors: Junji Kishino, Kohji Hanasaki, Toshiyuki Kato, Hitoshi Arita

    Abstract:

    Abstract The effects of throm☐ane A 2 (TXA 2 ) on the synthesis of platelet-activating factor (PAF) and leukotriene B 4 (LTB 4 ) were studied using human polymorphonuclear neutrophils (PMN). Scatchard analysis for binding experiments using [ 3 H]S-145, a specific TXA 2 /prostaglandin H 2 (PGH 2 ) receptor antagonist, revealed the existence of a single class of binding sites ( K d = 83.0 ± 2.8 nM, B max = 113.0 ± 3.1 fmol/2·10 6 cells) in human PMN. Upon stimulation with a combination of U46619, a TXA 2 mimetic agonist, and N -formylmethionylleucylphenylalanine (FMLP, 1 μM), the synthesis of PAF was detected, although this was not signficantly enhanced by U46619 or FMLP alone. The maximal production of PAF as well as the maximal activity of Acetyl-CoA Acetyltransferase was observed at approx. 20 min after addition of both stimuli. The effects of U46619 plus FMLP on PAF synthesis showed dose dependence to different concentrations of U46619 (0.1–10 μM), and were completely inhibited by S-145. Contrarily, no significant amounts of LTB 4 were detected by radioimmunoassay during the stimulation with U46619 and FMLP. These results suggest that TXA 2 and FMLP synergistically activate human PMN to induce PAF synthesis and this effect of TXA 2 is mediated through its specific receptor.

Keizo Waku – 3rd expert on this subject based on the ideXlab platform

  • Transient activation of 1-O-alkyl-sn-glycero-3-phosphocholine: Acetyl-CoA Acetyltransferase during the incubation of macrophages
    Lipids, 1991
    Co-Authors: Takayuki Sugiura, Teruo Fukuda, Neng-neng Cheng, Keizo Waku

    Abstract:

    The activity of the platelet-activating factor (PAF)-synthesizing enzyme, 1- O -alkyl- sn -glycero-3-phosphocholine (lysoPAF):Acetyl-CoA Acetyltransferase (EC 2.3.1.67) in alveolar macrophage lysate was found to be elevated after warming the cells to 37°C. Such an increase in enzyme activity was detectable only when intact cells were warmed. The stimulation was transient, reaching a peak at 2 min, and then gradually decreased to the control level. We could not find increased PAF formation in warmed cells which had increased Acetyltransferase activity, even though substantial amounts of lysoPAF were shown to be present within cells. In contrast, considerable amounts of PAF were formed after treatments of the cells with exogenous lysoPAF. These results suggest that the activation of Acetyltransferase is not sufficient to induce PAF formation and that the increased availability of substrates, especially lysoPAF, in the cells is indispensable for triggering PAF biosynthesis in this type of cells.