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Michael T Sivajothy – 1st expert on this subject based on the ideXlab platform
an advantage for young sperm in the house cricket Acheta domesticusThe American Naturalist, 2005Co-Authors: Klaus Reinhardt, Michael T SivajothyAbstract:
Abstract: We show that males of the house cricket Acheta domesticus regularly expel sperm packages (spermatophores) independently of copulation and at a rate that is not affected by the presence of females. We then show for the first time that the age of sperm affects their likelihood of being stored by females after copulation; younger sperm were overrepresented in the female sperm storage organ and therefore in the sperm population used for fertilization. Our results suggest that the reproductive success of males may increase if they deliver ejaculates with young sperm, and the results may explain why the males of several species are regularly observed to discard ejaculates. Our results also suggest that phenomena such as female multiple mating, paternity bias, and/or exaggerated ejaculate sizes may be related to the advantage both genders gain by using young sperm.
M.s. Kaulenas – 2nd expert on this subject based on the ideXlab platform
Analysis of the apparent tissue specific differences in Acheta domesticus ribosomal proteinsInsect Biochemistry, 2003Co-Authors: R.a. Bosselman, M.s. KaulenasAbstract:
Abstract One- and two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins isolated from Acheta domesticus male accessory gland, testes and whole body show highly reproducible quantitative and qualitative differences. However, control experiments show that these differences are due either to preferential loss of some ribosomal proteins or to the binding of cell sap proteins during ribosome isolation. No differences could be detected between proteins of 0.25 M and 0.5 M KCl-washed nymph and adult accessory gland ribosomes. The protein composition of ribosomes isolated from various tissues and developmental stages of the cricket appear to be very similar.
Ribosomal proteins of the cricket, Acheta domesticus L.Insect Biochemistry, 2003Co-Authors: R.a. Bosselman, M.s. KaulenasAbstract:
The ribosomal proteins of the house cricket, Acheta domesticus, have been characterized using one and two dimensional polyacrylamide gel electrophoresis. Ribosomes isolated from the adult male accessory gland and pelleted through a sucrose cushion contain 85 proteins. Ribosomal monomers from the same source isolated using density gradient centrifugation contain 76 proteins, while large and small subunits contain 38 and 30 proteins, respectively. The molecular weight of proteins associated with ribosomal monomers ranged from 6200 to 77,000. Large subunit proteins had molecular weights ranging from 7600 to 77,000 while small subunit proteins ranged from 8200 to 42,000.
Zonal sedimentation of ribosomal monomers through buffer containing 0.5 M KCl resulted in preparations containing 65 proteins. The use of the protease inhibitor, phenylmethylsulfonyl fluoride had no effect on the number or distribution of proteins in gradient purified monomers.
A notable feature of undissociated ribosomal monomers is the retention of a number of very low molecular weight proteins of unknown significance; most of these proteins do not appear to be degradation products.
Maria Augustyniak – 3rd expert on this subject based on the ideXlab platform
chronic toxicity of nanodiamonds can disturb development and reproduction of Acheta domesticus lEnvironmental Research, 2018Co-Authors: Julia Karpetakaczmarek, Andrzej Kedziorski, M A Augustyniakjablokow, Marta Dziewiecka, Maria AugustyniakAbstract:
Abstract The use of nanodiamonds in numerous materials designed for industry and medicine is growing rapidly. Consequently health and environmental risks associated with the exposure of humans and other biota to nanodiamonds-based materials are of the utmost importance. Scarcity of toxicological data for these particles led us to examine the potentially deleterious effects of nanodiamonds in model insect species, Acheta domesticus (Orthoptera) chronically exposed to ND in its diet. Organism-level end-point indices (lifespan, body weight, consumption, caloric value of faeces, reproduction) revealed adverse changes in the treated crickets in comparison with the control. Preliminary studies of oxidative stress level in the offspring of ND-treated crickets suggest toxicity of these particles limited to the exposed individuals. EPR analysis showing increase of radical signal in the faeces of ND-fed crickets led us to propose novel mechanism of nanodiamonds toxicity that is discussed in the light of literature data. Capsule Development and reproduction of Acheta domesticus can be disturbed by the chronic exposure to nanodiamonds