The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
Leif Rilfors - One of the best experts on this subject based on the ideXlab platform.
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The effects of hydration and divalent cations on lamellar-nonlamellar phase transitions in membranes and total lipid extracts from Acholeplasma laidlawii A-EF22 - a 2H NMR study.
2008Co-Authors: A. E. Niemi, A-s Andersson, Leif Rilfors, Göran Lindblom, Gösta ArvidsonAbstract:The effects of hydration and divalent cations on lamellar-nonlamellar phase transitions in membranes and total lipid extracts from Acholeplasma laidlawii A-EF22 - a 2H NMR study.
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structures of glucolipids from the membrane of Acholeplasma laidlawii strain a ef22 ii monoacylmonoglucosyldiacylglycerol
Biochimica et Biophysica Acta, 1994Co-Authors: Jon Hauksson, Göran Lindblom, Leif RilforsAbstract:Abstract The structure of one glucolipid from the membrane of Acholeplasma laidlawii, strain A-EF22, was determined. This glucolipid is synthesized only when a large fraction of saturated, straight-chain fatty acids are incorporated into the membrane lipids of strain A-EF22. The lipid was studied by 1H- and 13C-NMR spectroscopy. The structure of the lipid is 1,2- diacyl -3-O-[6-O- acyl-(α- d -glucopyranosyl )]-sn- glycerol . The result for this lipid shows that a previously published structure, based on incomplete chemical analyses, was incorrect. The phase equilibria for 1,2- diacyl -3-O-[6-O- acyl-(α- d -glucopyranosyl)] -sn- glycerol and the two dominating lipids in A. laidlawii, monoglucosyldiacylglycerol and diglucosyldiacylglycerol, are discussed and related to the chemical structure of the lipids.
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structures of glucolipids from the membrane of Acholeplasma laidlawii strain a ef22 i glycerophosphoryldiglucosyldiacylglycerol and monoacylbisglycerophosphoryldiglucosyldiacylglycerol
Biochimica et Biophysica Acta, 1994Co-Authors: Jon Hauksson, Göran Lindblom, Leif RilforsAbstract:Abstract The structures of two phosphoglucolipids from the membrane of Acholeplasma laidlawii , strain A-EF22 were determined by high resolution 13 C-, 31 P- and 1 H-NMR. The lipids in question are 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α- d -glucopyranosyl-(1 → 2)-O-α- d -glucopyranosyl)]-sn-glycerol ( 1 ) and 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α- d -glucopyranosyl-(1 → 2)-monoacyl-glycerophosphory]-6-O-α- d -glucopyranosyl)]-sn-glycerol (2). Both lipids are thus derivatives of diglucosyldiacylglycerol. Previous reports on these lipids, based on insufficient chemical analyses, showed contradictory structures. A phosphoglycolipid having the structure of 2 has not been described previously.
Alexey A Mouzykantov - One of the best experts on this subject based on the ideXlab platform.
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genome sequences of Acholeplasma laidlawii strains with increased resistance to tetracycline and melittin
Genome Announcements, 2018Co-Authors: E S Medvedeva, Maria N Siniagina, Eugenia A Boulygina, Tatyana Y Malygina, Natalia B Baranova, Mohamed Amine Dramchini, Reshad Akbor Prottoy, Alexey A MouzykantovAbstract:ABSTRACT Acholeplasma laidlawii is a well-suited model for studying the molecular basis for adapting mollicutes to environmental conditions. Here, we present the whole-genome sequences of two strains of A. laidlawii with increased resistance to tetracycline and melittin.
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genome sequences of Acholeplasma laidlawii strains differing in sensitivity to ciprofloxacin
Genome Announcements, 2017Co-Authors: Alexey A Mouzykantov, E S Medvedeva, Maria N Siniagina, Sergey Malanin, Eugenia A Boulygina, Tatyana Y Malygina, Natalia B BaranovaAbstract:ABSTRACT Acholeplasma laidlawii is a well-suited model for study of the molecular basis of the adaptation of mollicutes to environmental conditions. Here we present the whole-genome sequences of four strains of A. laidlawii with differential sensitivity to ciprofloxacin.
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extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii pg8
The Scientific World Journal, 2012Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, N B Baranova, O V Gorshkov, Maxim V Trushin, Tatiana N Nesterova, A A PonomarevaAbstract:For the first time, the phytopathogenicity of extracellular vesicles of Acholeplasma laidlawii PG8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) in Oryza sativa L. plants was studied. Data on the ability of extracellular vesicles of Acholeplasma laidlawii PG8 to penetrate from the nutrient medium into overground parts of Oryza sativa L. through the root system and to cause alterations in ultrastructural organization of the plants were presented. As a result of the analysis of ultrathin leaf sections of plants grown in medium with A. laidlawii PG8 vesicles, we detected significant changes in tissue ultrastructure characteristic to oxidative stress in plants as well as their cultivation along with bacterial cells. The presence of nucleotide sequences of some mycoplasma genes within extracellular vesicles of Acholeplasma laidlawii PG8 allowed a possibility to use PCR (with the following sequencing) to perform differential detection of cells and bacterial vesicles in samples under study. The obtained data may suggest the ability of extracellular vesicles of the mycoplasma to display in plants the features of infection from the viewpoint of virulence criteria—invasivity, infectivity—and toxigenicity—and to favor to bacterial phytopathogenicity.
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extracellular vesicles derived from Acholeplasma laidlawii pg8
The Scientific World Journal, 2011Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, E S Medvedeva, Irina R Efimova, Gulnara F Shaymardanova, Maxim V TrushinAbstract:Extracellular vesicle production is believed to be a ubiquitous process in bacteria, but the data on such a process in Mollicutes are absent. We report the isolation of ultramicroforms – extracellular vesicles from supernatants of Acholeplasma laidlawii PG8 (ubiquitous mycoplasma; the main contaminant of cell culture). Considering sizes, morphology, and ultrastructural organization, the ultramicroforms of A. laidlawii PG8 are similar to membrane vesicles of Gram-positive and Gram-negative bacteria. We demonstrate that A. laidlawii PG8 vesicles contain genetic material and proteins, and are mutagenic to lymphocytes of human peripheral blood. We show that Mycoplasma gallisepticum S6, the other mycoplasma, also produce similar structures, which suggests that shedding of the vesicles might be the common phenomenon in Mollicutes. We found that the action of stress conditions results in the intensive formation of ultramicroforms in mycoplasmas. The role of vesicular formation in mycoplasmas remains to be studied.
Göran Lindblom - One of the best experts on this subject based on the ideXlab platform.
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The effects of hydration and divalent cations on lamellar-nonlamellar phase transitions in membranes and total lipid extracts from Acholeplasma laidlawii A-EF22 - a 2H NMR study.
2008Co-Authors: A. E. Niemi, A-s Andersson, Leif Rilfors, Göran Lindblom, Gösta ArvidsonAbstract:The effects of hydration and divalent cations on lamellar-nonlamellar phase transitions in membranes and total lipid extracts from Acholeplasma laidlawii A-EF22 - a 2H NMR study.
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structures of glucolipids from the membrane of Acholeplasma laidlawii strain a ef22 ii monoacylmonoglucosyldiacylglycerol
Biochimica et Biophysica Acta, 1994Co-Authors: Jon Hauksson, Göran Lindblom, Leif RilforsAbstract:Abstract The structure of one glucolipid from the membrane of Acholeplasma laidlawii, strain A-EF22, was determined. This glucolipid is synthesized only when a large fraction of saturated, straight-chain fatty acids are incorporated into the membrane lipids of strain A-EF22. The lipid was studied by 1H- and 13C-NMR spectroscopy. The structure of the lipid is 1,2- diacyl -3-O-[6-O- acyl-(α- d -glucopyranosyl )]-sn- glycerol . The result for this lipid shows that a previously published structure, based on incomplete chemical analyses, was incorrect. The phase equilibria for 1,2- diacyl -3-O-[6-O- acyl-(α- d -glucopyranosyl)] -sn- glycerol and the two dominating lipids in A. laidlawii, monoglucosyldiacylglycerol and diglucosyldiacylglycerol, are discussed and related to the chemical structure of the lipids.
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structures of glucolipids from the membrane of Acholeplasma laidlawii strain a ef22 i glycerophosphoryldiglucosyldiacylglycerol and monoacylbisglycerophosphoryldiglucosyldiacylglycerol
Biochimica et Biophysica Acta, 1994Co-Authors: Jon Hauksson, Göran Lindblom, Leif RilforsAbstract:Abstract The structures of two phosphoglucolipids from the membrane of Acholeplasma laidlawii , strain A-EF22 were determined by high resolution 13 C-, 31 P- and 1 H-NMR. The lipids in question are 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α- d -glucopyranosyl-(1 → 2)-O-α- d -glucopyranosyl)]-sn-glycerol ( 1 ) and 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α- d -glucopyranosyl-(1 → 2)-monoacyl-glycerophosphory]-6-O-α- d -glucopyranosyl)]-sn-glycerol (2). Both lipids are thus derivatives of diglucosyldiacylglycerol. Previous reports on these lipids, based on insufficient chemical analyses, showed contradictory structures. A phosphoglycolipid having the structure of 2 has not been described previously.
V M Chernov - One of the best experts on this subject based on the ideXlab platform.
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extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii pg8
The Scientific World Journal, 2012Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, N B Baranova, O V Gorshkov, Maxim V Trushin, Tatiana N Nesterova, A A PonomarevaAbstract:For the first time, the phytopathogenicity of extracellular vesicles of Acholeplasma laidlawii PG8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) in Oryza sativa L. plants was studied. Data on the ability of extracellular vesicles of Acholeplasma laidlawii PG8 to penetrate from the nutrient medium into overground parts of Oryza sativa L. through the root system and to cause alterations in ultrastructural organization of the plants were presented. As a result of the analysis of ultrathin leaf sections of plants grown in medium with A. laidlawii PG8 vesicles, we detected significant changes in tissue ultrastructure characteristic to oxidative stress in plants as well as their cultivation along with bacterial cells. The presence of nucleotide sequences of some mycoplasma genes within extracellular vesicles of Acholeplasma laidlawii PG8 allowed a possibility to use PCR (with the following sequencing) to perform differential detection of cells and bacterial vesicles in samples under study. The obtained data may suggest the ability of extracellular vesicles of the mycoplasma to display in plants the features of infection from the viewpoint of virulence criteria—invasivity, infectivity—and toxigenicity—and to favor to bacterial phytopathogenicity.
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extracellular vesicles derived from Acholeplasma laidlawii pg8
The Scientific World Journal, 2011Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, E S Medvedeva, Irina R Efimova, Gulnara F Shaymardanova, Maxim V TrushinAbstract:Extracellular vesicle production is believed to be a ubiquitous process in bacteria, but the data on such a process in Mollicutes are absent. We report the isolation of ultramicroforms – extracellular vesicles from supernatants of Acholeplasma laidlawii PG8 (ubiquitous mycoplasma; the main contaminant of cell culture). Considering sizes, morphology, and ultrastructural organization, the ultramicroforms of A. laidlawii PG8 are similar to membrane vesicles of Gram-positive and Gram-negative bacteria. We demonstrate that A. laidlawii PG8 vesicles contain genetic material and proteins, and are mutagenic to lymphocytes of human peripheral blood. We show that Mycoplasma gallisepticum S6, the other mycoplasma, also produce similar structures, which suggests that shedding of the vesicles might be the common phenomenon in Mollicutes. We found that the action of stress conditions results in the intensive formation of ultramicroforms in mycoplasmas. The role of vesicular formation in mycoplasmas remains to be studied.
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Acholeplasma laidlawii pg8 culture adapted to unfavorable growth conditions shows an expressed phytopathogenicity
The Scientific World Journal, 2007Co-Authors: V M Chernov, Maxim V Trushin, Tatiana N Nesterova, Natalia E Moukhametshina, Yurii V Gogolev, O A ChernovaAbstract:Mycoplasmas are the smallest, self-replicating, prokaryotic organisms with avid biochemical potential and spreading in higher eukaryotes in nature. In this study, Acholeplasma laidlawii PG8 cells were cultivated on a deficient medium for 480 days resulting in a mycoplasma culture that was adapted in vitro to unfavorable growth conditions. Cells that survive this condition had decreased sizes (about 0.2 μm) and increased phytopathogenicity. This resulted in more frequent appearance of various morphological alterations when plants of vinca (Vinca minor L.) were infected by adapted mycoplasma cells. The increasing pathogenicity was accompanied by changes in genome expression in these adapted cells. Further studies are needed to explore the exact mechanisms that permit adaptation to unfavorable growth conditions and changes in phytopathogenic potential.
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the energetic stress response of the microalgal chlorella vulgaris to the mycoplasma Acholeplasma laidlawii as a model system for plant pathogen interaction
Thermochimica Acta, 2003Co-Authors: N.l. Loseva, V M Chernov, A A Ponomareva, I.n. Andreyeva, Ju A Alyabyev, Kh L Gordon, O P Kolesnikov, R B KempAbstract:Loseva, N. L., Alyabyev, A. J., Gordon, L. K., Andreyeva, I. N., Kolesnikov, O. P., Ponomareva, A. A., Chernov, V. M., Kemp, R .B. (2003). The energetic stress response of the microalgal Chlorella vulgaris to the mycoplasma, Acholeplasma laidlawii as a model system for plant-pathogen interaction. Thermochimica Acta, 397, (1-2), 37-47. Sponsorship: INTAS Grant no. 99-1390
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Direct and indirect calorimetric studies of stress responses of chlorella cells to infection with the mycoplasma, Acholeplasma laidlawii
Thermochimica Acta, 2002Co-Authors: N.l. Loseva, V M Chernov, O A Chernova, Lynn K. Gordon, Farida V. Minibayeva, A.ju. Alyabyev, I.n. Andreyeva, G.g. Rachimova, V.i. Tribunskih, R.i. EstrinaAbstract:Loseva, N. L., Gordon, L. K., Minibayeva, F. V., Alyabyev, A. J., Chernov, V. M., Chernova, O. A., Andreyeva, I. N., Rachimova, G. G., Tribunskih, V. I., Estrina, R. I., Gogolev, J. B., Kemp, R. B. (2002). Direct and indirect calorimetric studies of stress responses of chlorella cells to infection with the mycoplasma, Acholeplasma laidlawii. Thermochimica Acta, 390, (1-2), 39-46 Sponsorship: INTAS project, Ref. no.: 99-01390
Jon Hauksson - One of the best experts on this subject based on the ideXlab platform.
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structures of glucolipids from the membrane of Acholeplasma laidlawii strain a ef22 ii monoacylmonoglucosyldiacylglycerol
Biochimica et Biophysica Acta, 1994Co-Authors: Jon Hauksson, Göran Lindblom, Leif RilforsAbstract:Abstract The structure of one glucolipid from the membrane of Acholeplasma laidlawii, strain A-EF22, was determined. This glucolipid is synthesized only when a large fraction of saturated, straight-chain fatty acids are incorporated into the membrane lipids of strain A-EF22. The lipid was studied by 1H- and 13C-NMR spectroscopy. The structure of the lipid is 1,2- diacyl -3-O-[6-O- acyl-(α- d -glucopyranosyl )]-sn- glycerol . The result for this lipid shows that a previously published structure, based on incomplete chemical analyses, was incorrect. The phase equilibria for 1,2- diacyl -3-O-[6-O- acyl-(α- d -glucopyranosyl)] -sn- glycerol and the two dominating lipids in A. laidlawii, monoglucosyldiacylglycerol and diglucosyldiacylglycerol, are discussed and related to the chemical structure of the lipids.
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structures of glucolipids from the membrane of Acholeplasma laidlawii strain a ef22 i glycerophosphoryldiglucosyldiacylglycerol and monoacylbisglycerophosphoryldiglucosyldiacylglycerol
Biochimica et Biophysica Acta, 1994Co-Authors: Jon Hauksson, Göran Lindblom, Leif RilforsAbstract:Abstract The structures of two phosphoglucolipids from the membrane of Acholeplasma laidlawii , strain A-EF22 were determined by high resolution 13 C-, 31 P- and 1 H-NMR. The lipids in question are 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α- d -glucopyranosyl-(1 → 2)-O-α- d -glucopyranosyl)]-sn-glycerol ( 1 ) and 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α- d -glucopyranosyl-(1 → 2)-monoacyl-glycerophosphory]-6-O-α- d -glucopyranosyl)]-sn-glycerol (2). Both lipids are thus derivatives of diglucosyldiacylglycerol. Previous reports on these lipids, based on insufficient chemical analyses, showed contradictory structures. A phosphoglycolipid having the structure of 2 has not been described previously.