Acholeplasma laidlawii

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Leif Rilfors - One of the best experts on this subject based on the ideXlab platform.

Alexey A Mouzykantov - One of the best experts on this subject based on the ideXlab platform.

  • genome sequences of Acholeplasma laidlawii strains with increased resistance to tetracycline and melittin
    Genome Announcements, 2018
    Co-Authors: E S Medvedeva, Maria N Siniagina, Eugenia A Boulygina, Tatyana Y Malygina, Natalia B Baranova, Mohamed Amine Dramchini, Reshad Akbor Prottoy, Alexey A Mouzykantov
    Abstract:

    ABSTRACT Acholeplasma laidlawii is a well-suited model for studying the molecular basis for adapting mollicutes to environmental conditions. Here, we present the whole-genome sequences of two strains of A. laidlawii with increased resistance to tetracycline and melittin.

  • genome sequences of Acholeplasma laidlawii strains differing in sensitivity to ciprofloxacin
    Genome Announcements, 2017
    Co-Authors: Alexey A Mouzykantov, E S Medvedeva, Maria N Siniagina, Sergey Malanin, Eugenia A Boulygina, Tatyana Y Malygina, Natalia B Baranova
    Abstract:

    ABSTRACT Acholeplasma laidlawii is a well-suited model for study of the molecular basis of the adaptation of mollicutes to environmental conditions. Here we present the whole-genome sequences of four strains of A. laidlawii with differential sensitivity to ciprofloxacin.

  • extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii pg8
    The Scientific World Journal, 2012
    Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, N B Baranova, O V Gorshkov, Maxim V Trushin, Tatiana N Nesterova, A A Ponomareva
    Abstract:

    For the first time, the phytopathogenicity of extracellular vesicles of Acholeplasma laidlawii PG8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) in Oryza sativa L. plants was studied. Data on the ability of extracellular vesicles of Acholeplasma laidlawii PG8 to penetrate from the nutrient medium into overground parts of Oryza sativa L. through the root system and to cause alterations in ultrastructural organization of the plants were presented. As a result of the analysis of ultrathin leaf sections of plants grown in medium with A. laidlawii PG8 vesicles, we detected significant changes in tissue ultrastructure characteristic to oxidative stress in plants as well as their cultivation along with bacterial cells. The presence of nucleotide sequences of some mycoplasma genes within extracellular vesicles of Acholeplasma laidlawii PG8 allowed a possibility to use PCR (with the following sequencing) to perform differential detection of cells and bacterial vesicles in samples under study. The obtained data may suggest the ability of extracellular vesicles of the mycoplasma to display in plants the features of infection from the viewpoint of virulence criteria—invasivity, infectivity—and toxigenicity—and to favor to bacterial phytopathogenicity.

  • extracellular vesicles derived from Acholeplasma laidlawii pg8
    The Scientific World Journal, 2011
    Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, E S Medvedeva, Irina R Efimova, Gulnara F Shaymardanova, Maxim V Trushin
    Abstract:

    Extracellular vesicle production is believed to be a ubiquitous process in bacteria, but the data on such a process in Mollicutes are absent. We report the isolation of ultramicroforms – extracellular vesicles from supernatants of Acholeplasma laidlawii PG8 (ubiquitous mycoplasma; the main contaminant of cell culture). Considering sizes, morphology, and ultrastructural organization, the ultramicroforms of A. laidlawii PG8 are similar to membrane vesicles of Gram-positive and Gram-negative bacteria. We demonstrate that A. laidlawii PG8 vesicles contain genetic material and proteins, and are mutagenic to lymphocytes of human peripheral blood. We show that Mycoplasma gallisepticum S6, the other mycoplasma, also produce similar structures, which suggests that shedding of the vesicles might be the common phenomenon in Mollicutes. We found that the action of stress conditions results in the intensive formation of ultramicroforms in mycoplasmas. The role of vesicular formation in mycoplasmas remains to be studied.

Göran Lindblom - One of the best experts on this subject based on the ideXlab platform.

V M Chernov - One of the best experts on this subject based on the ideXlab platform.

  • extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii pg8
    The Scientific World Journal, 2012
    Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, N B Baranova, O V Gorshkov, Maxim V Trushin, Tatiana N Nesterova, A A Ponomareva
    Abstract:

    For the first time, the phytopathogenicity of extracellular vesicles of Acholeplasma laidlawii PG8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) in Oryza sativa L. plants was studied. Data on the ability of extracellular vesicles of Acholeplasma laidlawii PG8 to penetrate from the nutrient medium into overground parts of Oryza sativa L. through the root system and to cause alterations in ultrastructural organization of the plants were presented. As a result of the analysis of ultrathin leaf sections of plants grown in medium with A. laidlawii PG8 vesicles, we detected significant changes in tissue ultrastructure characteristic to oxidative stress in plants as well as their cultivation along with bacterial cells. The presence of nucleotide sequences of some mycoplasma genes within extracellular vesicles of Acholeplasma laidlawii PG8 allowed a possibility to use PCR (with the following sequencing) to perform differential detection of cells and bacterial vesicles in samples under study. The obtained data may suggest the ability of extracellular vesicles of the mycoplasma to display in plants the features of infection from the viewpoint of virulence criteria—invasivity, infectivity—and toxigenicity—and to favor to bacterial phytopathogenicity.

  • extracellular vesicles derived from Acholeplasma laidlawii pg8
    The Scientific World Journal, 2011
    Co-Authors: V M Chernov, O A Chernova, Alexey A Mouzykantov, E S Medvedeva, Irina R Efimova, Gulnara F Shaymardanova, Maxim V Trushin
    Abstract:

    Extracellular vesicle production is believed to be a ubiquitous process in bacteria, but the data on such a process in Mollicutes are absent. We report the isolation of ultramicroforms – extracellular vesicles from supernatants of Acholeplasma laidlawii PG8 (ubiquitous mycoplasma; the main contaminant of cell culture). Considering sizes, morphology, and ultrastructural organization, the ultramicroforms of A. laidlawii PG8 are similar to membrane vesicles of Gram-positive and Gram-negative bacteria. We demonstrate that A. laidlawii PG8 vesicles contain genetic material and proteins, and are mutagenic to lymphocytes of human peripheral blood. We show that Mycoplasma gallisepticum S6, the other mycoplasma, also produce similar structures, which suggests that shedding of the vesicles might be the common phenomenon in Mollicutes. We found that the action of stress conditions results in the intensive formation of ultramicroforms in mycoplasmas. The role of vesicular formation in mycoplasmas remains to be studied.

  • Acholeplasma laidlawii pg8 culture adapted to unfavorable growth conditions shows an expressed phytopathogenicity
    The Scientific World Journal, 2007
    Co-Authors: V M Chernov, Maxim V Trushin, Tatiana N Nesterova, Natalia E Moukhametshina, Yurii V Gogolev, O A Chernova
    Abstract:

    Mycoplasmas are the smallest, self-replicating, prokaryotic organisms with avid biochemical potential and spreading in higher eukaryotes in nature. In this study, Acholeplasma laidlawii PG8 cells were cultivated on a deficient medium for 480 days resulting in a mycoplasma culture that was adapted in vitro to unfavorable growth conditions. Cells that survive this condition had decreased sizes (about 0.2 μm) and increased phytopathogenicity. This resulted in more frequent appearance of various morphological alterations when plants of vinca (Vinca minor L.) were infected by adapted mycoplasma cells. The increasing pathogenicity was accompanied by changes in genome expression in these adapted cells. Further studies are needed to explore the exact mechanisms that permit adaptation to unfavorable growth conditions and changes in phytopathogenic potential.

  • the energetic stress response of the microalgal chlorella vulgaris to the mycoplasma Acholeplasma laidlawii as a model system for plant pathogen interaction
    Thermochimica Acta, 2003
    Co-Authors: N.l. Loseva, V M Chernov, A A Ponomareva, I.n. Andreyeva, Ju A Alyabyev, Kh L Gordon, O P Kolesnikov, R B Kemp
    Abstract:

    Loseva, N. L., Alyabyev, A. J., Gordon, L. K., Andreyeva, I. N., Kolesnikov, O. P., Ponomareva, A. A., Chernov, V. M., Kemp, R .B. (2003). The energetic stress response of the microalgal Chlorella vulgaris to the mycoplasma, Acholeplasma laidlawii as a model system for plant-pathogen interaction. Thermochimica Acta, 397, (1-2), 37-47. Sponsorship: INTAS Grant no. 99-1390

  • Direct and indirect calorimetric studies of stress responses of chlorella cells to infection with the mycoplasma, Acholeplasma laidlawii
    Thermochimica Acta, 2002
    Co-Authors: N.l. Loseva, V M Chernov, O A Chernova, Lynn K. Gordon, Farida V. Minibayeva, A.ju. Alyabyev, I.n. Andreyeva, G.g. Rachimova, V.i. Tribunskih, R.i. Estrina
    Abstract:

    Loseva, N. L., Gordon, L. K., Minibayeva, F. V., Alyabyev, A. J., Chernov, V. M., Chernova, O. A., Andreyeva, I. N., Rachimova, G. G., Tribunskih, V. I., Estrina, R. I., Gogolev, J. B., Kemp, R. B. (2002). Direct and indirect calorimetric studies of stress responses of chlorella cells to infection with the mycoplasma, Acholeplasma laidlawii. Thermochimica Acta, 390, (1-2), 39-46 Sponsorship: INTAS project, Ref. no.: 99-01390

Jon Hauksson - One of the best experts on this subject based on the ideXlab platform.