The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Baochang Cai - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous Extracts of raw and salt-processed Achyranthes Bidentata
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yi Tao, Yingshan Du, Weidong Li, Baochang CaiAbstract:A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes Bidentata Extract. The saponins were completely separated on a Waters BEH C 18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode. The sample preparations for protein removal were accomplished using a simple acetonitrile precipitation method. The calibration curves displayed good linearity (r 2 > 0.9998) with the concentration ranges of 24.4–6100 ng mL −1 , 25.6–6400 ng mL −1 , 20.4–8500 ng mL −1 , 21.6–5400 ng mL −1 , 21.6–6100 ng mL −1 for the five saponins, respectively. The intra-day and inter-day precisions (RSD) of the five saponins were less than 3.95% and the bias of the accuracies ranged from −4.50% to 4.84%. The Extraction recoveries of the five saponins ranged from 95.2% to 104.8% and the matrix effects were satisfactory. In comparison with the raw group, the parameters of C max and AUC 0-t of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, and chikusetsusaponin IVa elevated remarkably (p < 0.05) after oral delivery of the Extract of salt-processed Achyranthes Bidentata, which revealed that salt-processing could increase bioavailability of β-ecdysterone, 25S-inokosterone, ginsenoside Ro and chikusetsusaponin IVa.
Liekui Hu - One of the best experts on this subject based on the ideXlab platform.
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Achyranthes Bidentata Extract protects chondrocytes functions through suppressing glycolysis and apoptosis via mapk akt signaling axis
American Journal of Translational Research, 2020Co-Authors: Yuan Li, Wei Xiao, Liping Peng, Lixin Wang, Zhouwei Liao, Liekui HuAbstract:Osteoarthritis (OA) is considered to be a joint-associated disorder and one of leading reasons for disability, however, potential mechanism has never been clarified. The purpose of this research was to evaluate protective-effects of Achyranthes Bidentata Extracts (ABE) on chondrocytes function in osteoarthritis. We performed a systematic investigation of transcriptional and proteomic landscapes to identify the underlying mechanisms behind effects of ABE on chondrocytic functions. OA animal models were generated in the present research. Chondrocytes were isolated and cultured, and then prepared for GeneChip analysis. Two-dimensional gel electrophoresis and LC-MS/MS analysis were conducted to analyze samples. Quantitative real-time PCR (qRT-PCR) and western blotting were used to evaluate expression of protein kinase B (AKT), beta-tubulin and beta-action. Apoptosis and glycolysis pathway were significantly compromised in chondrocytes with ABE stimulation as revealed by both transcriptional and proteomic data. Consistently, ABE suppressed chondrocytes apoptosis and glycolytic activity in vitro through modulating multiple genes, such as Plk2, Casp1/12 and Cers1 as well as Pkm2, Eno1/3 and Pgk2. Mechanically, ABE activated MAPK signaling pathway and suppressed AKT signaling pathway, therefore, reducing the glycolysis to provide survival benefits. We extended our analysis by verifying insulin-like growth factor 1 (IGF-1) and MAP kinase 1 (MEK1) in chondrocytes function. Depletion of either IGF-1 or MEK1 impaired AKT expression and phosphorylation, leading to the enhanced chondrocyte apoptosis and reduced cell proliferation. In conclusion, our study provided systematic view and molecular basis for ABE to serve as potential intervention of OA via suppressing AKT signaling.
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Achyranthes Bidentata Extract protects chondrocytes functions through suppressing glycolysis and apoptosis via MAPK/AKT signaling axis.
American Journal of Translational Research, 2020Co-Authors: Dujun Ma, Yuan Li, Wei Xiao, Liping Peng, Lixin Wang, Zhouwei Liao, Liekui HuAbstract:Osteoarthritis (OA) is considered to be a joint-associated disorder and one of leading reasons for disability, however, potential mechanism has never been clarified. The purpose of this research was to evaluate protective-effects of Achyranthes Bidentata Extracts (ABE) on chondrocytes function in osteoarthritis. We performed a systematic investigation of transcriptional and proteomic landscapes to identify the underlying mechanisms behind effects of ABE on chondrocytic functions. OA animal models were generated in the present research. Chondrocytes were isolated and cultured, and then prepared for GeneChip analysis. Two-dimensional gel electrophoresis and LC-MS/MS analysis were conducted to analyze samples. Quantitative real-time PCR (qRT-PCR) and western blotting were used to evaluate expression of protein kinase B (AKT), beta-tubulin and beta-action. Apoptosis and glycolysis pathway were significantly compromised in chondrocytes with ABE stimulation as revealed by both transcriptional and proteomic data. Consistently, ABE suppressed chondrocytes apoptosis and glycolytic activity in vitro through modulating multiple genes, such as Plk2, Casp1/12 and Cers1 as well as Pkm2, Eno1/3 and Pgk2. Mechanically, ABE activated MAPK signaling pathway and suppressed AKT signaling pathway, therefore, reducing the glycolysis to provide survival benefits. We extended our analysis by verifying insulin-like growth factor 1 (IGF-1) and MAP kinase 1 (MEK1) in chondrocytes function. Depletion of either IGF-1 or MEK1 impaired AKT expression and phosphorylation, leading to the enhanced chondrocyte apoptosis and reduced cell proliferation. In conclusion, our study provided systematic view and molecular basis for ABE to serve as potential intervention of OA via suppressing AKT signaling.
Yi Tao - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous Extracts of raw and salt-processed Achyranthes Bidentata
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yi Tao, Yingshan Du, Weidong Li, Baochang CaiAbstract:A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes Bidentata Extract. The saponins were completely separated on a Waters BEH C 18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode. The sample preparations for protein removal were accomplished using a simple acetonitrile precipitation method. The calibration curves displayed good linearity (r 2 > 0.9998) with the concentration ranges of 24.4–6100 ng mL −1 , 25.6–6400 ng mL −1 , 20.4–8500 ng mL −1 , 21.6–5400 ng mL −1 , 21.6–6100 ng mL −1 for the five saponins, respectively. The intra-day and inter-day precisions (RSD) of the five saponins were less than 3.95% and the bias of the accuracies ranged from −4.50% to 4.84%. The Extraction recoveries of the five saponins ranged from 95.2% to 104.8% and the matrix effects were satisfactory. In comparison with the raw group, the parameters of C max and AUC 0-t of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, and chikusetsusaponin IVa elevated remarkably (p < 0.05) after oral delivery of the Extract of salt-processed Achyranthes Bidentata, which revealed that salt-processing could increase bioavailability of β-ecdysterone, 25S-inokosterone, ginsenoside Ro and chikusetsusaponin IVa.
Weidong Li - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous Extracts of raw and salt-processed Achyranthes Bidentata
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yingshan Du, Weidong LiAbstract:Abstract A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes Bidentata Extract. The saponins were completely separated on a Waters BEH C18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode. The sample preparations for protein removal were accomplished using a simple acetonitrile precipitation method. The calibration curves displayed good linearity (r2 > 0.9998) with the concentration ranges of 24.4–6100 ng mL−1, 25.6–6400 ng mL−1, 20.4–8500 ng mL−1, 21.6–5400 ng mL−1, 21.6–6100 ng mL−1 for the five saponins, respectively. The intra-day and inter-day precisions (RSD) of the five saponins were less than 3.95% and the bias of the accuracies ranged from −4.50% to 4.84%. The Extraction recoveries of the five saponins ranged from 95.2% to 104.8% and the matrix effects were satisfactory. In comparison with the raw group, the parameters of Cmax and AUC0-t of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, and chikusetsusaponin IVa elevated remarkably (p
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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous Extracts of raw and salt-processed Achyranthes Bidentata
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yi Tao, Yingshan Du, Weidong Li, Baochang CaiAbstract:A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes Bidentata Extract. The saponins were completely separated on a Waters BEH C 18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode. The sample preparations for protein removal were accomplished using a simple acetonitrile precipitation method. The calibration curves displayed good linearity (r 2 > 0.9998) with the concentration ranges of 24.4–6100 ng mL −1 , 25.6–6400 ng mL −1 , 20.4–8500 ng mL −1 , 21.6–5400 ng mL −1 , 21.6–6100 ng mL −1 for the five saponins, respectively. The intra-day and inter-day precisions (RSD) of the five saponins were less than 3.95% and the bias of the accuracies ranged from −4.50% to 4.84%. The Extraction recoveries of the five saponins ranged from 95.2% to 104.8% and the matrix effects were satisfactory. In comparison with the raw group, the parameters of C max and AUC 0-t of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, and chikusetsusaponin IVa elevated remarkably (p < 0.05) after oral delivery of the Extract of salt-processed Achyranthes Bidentata, which revealed that salt-processing could increase bioavailability of β-ecdysterone, 25S-inokosterone, ginsenoside Ro and chikusetsusaponin IVa.
Yingshan Du - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous Extracts of raw and salt-processed Achyranthes Bidentata
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yingshan Du, Weidong LiAbstract:Abstract A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes Bidentata Extract. The saponins were completely separated on a Waters BEH C18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode. The sample preparations for protein removal were accomplished using a simple acetonitrile precipitation method. The calibration curves displayed good linearity (r2 > 0.9998) with the concentration ranges of 24.4–6100 ng mL−1, 25.6–6400 ng mL−1, 20.4–8500 ng mL−1, 21.6–5400 ng mL−1, 21.6–6100 ng mL−1 for the five saponins, respectively. The intra-day and inter-day precisions (RSD) of the five saponins were less than 3.95% and the bias of the accuracies ranged from −4.50% to 4.84%. The Extraction recoveries of the five saponins ranged from 95.2% to 104.8% and the matrix effects were satisfactory. In comparison with the raw group, the parameters of Cmax and AUC0-t of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, and chikusetsusaponin IVa elevated remarkably (p
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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous Extracts of raw and salt-processed Achyranthes Bidentata
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yi Tao, Yingshan Du, Weidong Li, Baochang CaiAbstract:A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes Bidentata Extract. The saponins were completely separated on a Waters BEH C 18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode. The sample preparations for protein removal were accomplished using a simple acetonitrile precipitation method. The calibration curves displayed good linearity (r 2 > 0.9998) with the concentration ranges of 24.4–6100 ng mL −1 , 25.6–6400 ng mL −1 , 20.4–8500 ng mL −1 , 21.6–5400 ng mL −1 , 21.6–6100 ng mL −1 for the five saponins, respectively. The intra-day and inter-day precisions (RSD) of the five saponins were less than 3.95% and the bias of the accuracies ranged from −4.50% to 4.84%. The Extraction recoveries of the five saponins ranged from 95.2% to 104.8% and the matrix effects were satisfactory. In comparison with the raw group, the parameters of C max and AUC 0-t of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, and chikusetsusaponin IVa elevated remarkably (p < 0.05) after oral delivery of the Extract of salt-processed Achyranthes Bidentata, which revealed that salt-processing could increase bioavailability of β-ecdysterone, 25S-inokosterone, ginsenoside Ro and chikusetsusaponin IVa.
