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Otomar Linhart - One of the best experts on this subject based on the ideXlab platform.
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sperm maturation in sturgeon actinopterygii Acipenseriformes a review
Theriogenology, 2017Co-Authors: Borys Dzyuba, Marek Rodina, Otomar Linhart, Jacky Cosson, Viktoriya Dzyuba, P Fedorov, Olga Bondarenko, William L Shelton, Sergii BoryshpoletsAbstract:Abstract The morphology of the reproductive system of acipenseriform fishes is quite different from that of teleostean species, but an associated unique physiological difference in male sturgeons was not discovered until recently; sperm of sturgeons passes through the kidneys then via Wolffian ducts into the environment rather that emptying directly through seminal ducts. The mixing of sperm with excretory products has been found to be a requisite for the capacity to be activated (maturation step) instead of being deleterious. In the current review we summarize results of studies performed in our laboratory on physiological processes involved in sturgeon sperm maturation, namely changes in: 1) ionic environment; 2) sensitivity of spermatozoa to calcium ions (Ca2+); 3) antioxidant enzymes and proteolytic activities; and 4) content in macroergic phosphates arising during this maturation process. We also discuss taxa-specific aspects of sturgeon sperm maturation in relation to hormonal regulation of spermiation, and the unusual features of sturgeon sperm maturation relative to using testicular sturgeon sperm in aquaculture.
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Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Journal of Applied Ichthyology, 2011Co-Authors: Ping Li, Marek Rodina, Martin Hulak, David Gela, Martin Pšenička, Zhi-hua Li, Otomar LinhartAbstract:Summary Physico-chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P
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comparative protein profiles potential molecular markers from spermatozoa of Acipenseriformes chondrostei pisces
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2010Co-Authors: Ping Li, Marek Rodina, Martin Hulak, Zhi-hua Li, M Sulc, Otomar LinhartAbstract:Abstract Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon ( Acipenser baerii ), sterlet ( A. ruthenus ), Russian sturgeon ( A. gueldenstaedtii ), starry sturgeon ( A. stellatus ), beluga ( Huso huso ), and Mississippi paddlefish ( Polyodon spathula ). Protein profiles of spermatozoa were determined by isoelectric focusing and two-dimensional electrophoresis (2-DE) high-resolution gels. The peptides, previously selected by 2-DE analysis as potentially species-specific, were obtained by “in-gel” tryptic digestion, followed by matrix-associated laser desorption/ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. Exceptions were A. ruthenus and H. huso , species with a close phylogenetic relationship. Glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula . Phosphoglycerate kinase was detected only in A. ruthenus and H. huso , and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso . This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.
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ionic composition and osmolality of paddlefish polyodon spathula Acipenseriformes seminal fluid
Aquaculture International, 2003Co-Authors: Otomar Linhart, Jacky Cosson, William L Shelton, Steve D Mims, Boris Gomelsky, Ana E Hiott, M Rodina, D Gela, Jan BastlAbstract:Changes in ionic composition as Na+,K+, Ca2+ and Mg2+, osmolality inseminal fluid, percentage of motile spermatozoaand velocity were investigated in response toCPP and different dosage of LHRHa. The lowestvelocity of sperm was observed after use CPPtreatment. The velocity of spermatozoa,significant main effect of the treatment(P < 0.0001) and the time of sperm collection(P < 0.0104) were evaluated. The osmolality ofseminal fluid was different betweenexperimental groups of LHRHa (48.0–62.7mOsmol.kg−1) and CPP (33.0–46.3mOsmol.kg−1) treatments. The osmolalitywas significantly higher on the first day andone-half, then declined on day three, rangingfrom 33.0 to 62.7 mOsmol.kg−1. Analysisof variance showed significant main effects ofthe treatment (P < 0.0001) and the time ofsperm collection (P < 0.0002) on the osmolalityof seminal fluid. The level of Na+ andK+ ion was different between experimentalgroups of LHRHa and CPP treatment. The highestconcentration of 11.11 mmol.l−1 wasobserved at Na+ ion. Then theconcentrations declined on the level 1.56, 0.52and 0.36 mmol.l−1 for K+, Ca2+and Mg2+ ions, respectively. There werehighly positive correlations between osmolalityof seminal fluid and dosage of LHRHa treatment(r = 0.84), velocity of spermatozoa andosmolality of seminal fluid (r = 0.57) andosmolality of seminal fluid and Na+concentration at seminal fluid (r = 0.70).Injection with LHRHa increased quality of spermas velocity of sperm, level of Na+,K+ and osmolality at seminal fluidcompared to CPP treatments.
Michael J Conlon - One of the best experts on this subject based on the ideXlab platform.
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purification and characterization of galanin and scyliorhinin i from the hybrid sturgeon scaphirhynchus platorynchus scaphirhynchus albus Acipenseriformes
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Bruce A Barton, Per F. Nielsen, Lars Thim, Michael J ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
Marek Rodina - One of the best experts on this subject based on the ideXlab platform.
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sperm maturation in sturgeon actinopterygii Acipenseriformes a review
Theriogenology, 2017Co-Authors: Borys Dzyuba, Marek Rodina, Otomar Linhart, Jacky Cosson, Viktoriya Dzyuba, P Fedorov, Olga Bondarenko, William L Shelton, Sergii BoryshpoletsAbstract:Abstract The morphology of the reproductive system of acipenseriform fishes is quite different from that of teleostean species, but an associated unique physiological difference in male sturgeons was not discovered until recently; sperm of sturgeons passes through the kidneys then via Wolffian ducts into the environment rather that emptying directly through seminal ducts. The mixing of sperm with excretory products has been found to be a requisite for the capacity to be activated (maturation step) instead of being deleterious. In the current review we summarize results of studies performed in our laboratory on physiological processes involved in sturgeon sperm maturation, namely changes in: 1) ionic environment; 2) sensitivity of spermatozoa to calcium ions (Ca2+); 3) antioxidant enzymes and proteolytic activities; and 4) content in macroergic phosphates arising during this maturation process. We also discuss taxa-specific aspects of sturgeon sperm maturation in relation to hormonal regulation of spermiation, and the unusual features of sturgeon sperm maturation relative to using testicular sturgeon sperm in aquaculture.
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Fertility of a spontaneous hexaploid male Siberian sturgeon, Acipenser baerii
BMC Genetics, 2014Co-Authors: Miloš Havelka, Arne Ludwig, Marek Rodina, Martin Hulak, David Gela, Martin Pšenička, Marie Rábová, Dietmar Lieckfeldt, Dmytro BytyutskyyAbstract:Background Evolution of sturgeons and paddlefishes (order Acipenseriformes) is inherently connected with polyploidization events which resulted in differentiation of ploidy levels and chromosome numbers of present acipenseriform species. Moreover, allopolyploidization as well as autopolyploidization seems to be an ongoing process in these fishes and individuals with abnormal ploidy levels were occasionally observed within sturgeon populations. Here, we reported occurrence of Siberian sturgeon (Acipenser baerii) male with abnormal ploidy level for this species, accessed its ploidy level and chromosome number and investigate its potential sterility or fertility in comparison with normal individuals of sterlet (A. ruthenus), Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii).
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Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Journal of Applied Ichthyology, 2011Co-Authors: Ping Li, Marek Rodina, Martin Hulak, David Gela, Martin Pšenička, Zhi-hua Li, Otomar LinhartAbstract:Summary Physico-chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P
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comparative protein profiles potential molecular markers from spermatozoa of Acipenseriformes chondrostei pisces
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2010Co-Authors: Ping Li, Marek Rodina, Martin Hulak, Zhi-hua Li, M Sulc, Otomar LinhartAbstract:Abstract Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon ( Acipenser baerii ), sterlet ( A. ruthenus ), Russian sturgeon ( A. gueldenstaedtii ), starry sturgeon ( A. stellatus ), beluga ( Huso huso ), and Mississippi paddlefish ( Polyodon spathula ). Protein profiles of spermatozoa were determined by isoelectric focusing and two-dimensional electrophoresis (2-DE) high-resolution gels. The peptides, previously selected by 2-DE analysis as potentially species-specific, were obtained by “in-gel” tryptic digestion, followed by matrix-associated laser desorption/ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. Exceptions were A. ruthenus and H. huso , species with a close phylogenetic relationship. Glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula . Phosphoglycerate kinase was detected only in A. ruthenus and H. huso , and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso . This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.
Ping Li - One of the best experts on this subject based on the ideXlab platform.
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Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Journal of Applied Ichthyology, 2011Co-Authors: Ping Li, Marek Rodina, Martin Hulak, David Gela, Martin Pšenička, Zhi-hua Li, Otomar LinhartAbstract:Summary Physico-chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P
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comparative protein profiles potential molecular markers from spermatozoa of Acipenseriformes chondrostei pisces
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2010Co-Authors: Ping Li, Marek Rodina, Martin Hulak, Zhi-hua Li, M Sulc, Otomar LinhartAbstract:Abstract Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon ( Acipenser baerii ), sterlet ( A. ruthenus ), Russian sturgeon ( A. gueldenstaedtii ), starry sturgeon ( A. stellatus ), beluga ( Huso huso ), and Mississippi paddlefish ( Polyodon spathula ). Protein profiles of spermatozoa were determined by isoelectric focusing and two-dimensional electrophoresis (2-DE) high-resolution gels. The peptides, previously selected by 2-DE analysis as potentially species-specific, were obtained by “in-gel” tryptic digestion, followed by matrix-associated laser desorption/ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. Exceptions were A. ruthenus and H. huso , species with a close phylogenetic relationship. Glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula . Phosphoglycerate kinase was detected only in A. ruthenus and H. huso , and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso . This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.
Yuqi Wang - One of the best experts on this subject based on the ideXlab platform.
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Purification and Characterization of Galanin and Scyliorhinin I from the Hybrid Sturgeon,Scaphirhynchus platorynchus×Scaphirhynchus albus(Acipenseriformes) ☆
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Bruce A Barton, Per F. Nielsen, Lars Thim, J. Michael ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
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purification and characterization of galanin and scyliorhinin i from the hybrid sturgeon scaphirhynchus platorynchus scaphirhynchus albus Acipenseriformes
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Bruce A Barton, Per F. Nielsen, Lars Thim, Michael J ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
