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Carlos Augusto Colombo - One of the best experts on this subject based on the ideXlab platform.
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development of novel est ssr markers in the macauba palm Acrocomia aculeata using transcriptome sequencing and cross species transferability in arecaceae species
BMC Plant Biology, 2018Co-Authors: Bárbara Bazzo, Marcelo Carazzolle, Lucas Miguel De Carvalho, Gonçalo Amarante Guimarães Pereira, Carlos Augusto ColomboAbstract:The macauba palm is a novel feedstock for oil production suitable for multiple uses, including as biodiesel and in the food and cosmetic industries. As an efficient alternative, the macauba palm has limited genomic resources, particularly expressed sequence tag (EST) markers. We report a comprehensive set of validated EST-simple sequence repeat (SSR) markers by using transcriptome sequencing, its application in genetic diversity analysis and cross transferability in other palm trees with environmental and economic importance. In this study, a total of 418 EST-SSRs were identified to be unique for one transcript and region; 232 EST-SSRs were selected, with trinucleotide repeats being the most frequent motif, representing 380 (90.9%), followed by composited (4.5%), di- (3.6%), and hexanucleotides (3.6%). A total of 145 EST-SSRs (62.5%) were validated for consistent amplification in seventeen macauba palm samples, and 100 were determined to be polymorphic with PIC values ranging from 0.25 to 0.77. Genetic diversity analysis was performed with the 20 most informative EST-SSR markers showing a distinct separation of the different groups of macauba palm. Additionally, these 145 markers were transferred in six other palm species resulting in transferability rates of 99% (144) in Acrocomia intumescens, 98% (143) in Acrocomia totai, 80.7% (117 EST-EST) in African oil palm (Elaeis guineensis) and peach palm (Bactris gasipaes) samples, 70% (102) in the jucara palm (Euterpe edulis) and 71.7% (104) in the hat palm (Sabal causiarum). Analysis of genetic distance showed a high separation in accordance with geographic location, establishing distinct groups by genera. The EST markers identified in our study are a valuable resource and provide a genomic tool for genetic mapping and further genetic studies, as well as evaluation of co-location between QTLs and functionally associated markers.
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Development of novel EST-SSR markers in the macaúba palm (Acrocomia aculeata) using transcriptome sequencing and cross-species transferability in Arecaceae species
BMC, 2018Co-Authors: Bárbara Bazzo, Marcelo Carazzolle, Lucas Miguel De Carvalho, Gonçalo Amarante Guimarães Pereira, Carlos Augusto ColomboAbstract:Abstract Background The macaúba palm is a novel feedstock for oil production suitable for multiple uses, including as biodiesel and in the food and cosmetic industries. As an efficient alternative, the macaúba palm has limited genomic resources, particularly expressed sequence tag (EST) markers. We report a comprehensive set of validated EST-simple sequence repeat (SSR) markers by using transcriptome sequencing, its application in genetic diversity analysis and cross transferability in other palm trees with environmental and economic importance. Results In this study, a total of 418 EST-SSRs were identified to be unique for one transcript and region; 232 EST-SSRs were selected, with trinucleotide repeats being the most frequent motif, representing 380 (90.9%), followed by composited (4.5%), di- (3.6%), and hexanucleotides (3.6%). A total of 145 EST-SSRs (62.5%) were validated for consistent amplification in seventeen macaúba palm samples, and 100 were determined to be polymorphic with PIC values ranging from 0.25 to 0.77. Genetic diversity analysis was performed with the 20 most informative EST-SSR markers showing a distinct separation of the different groups of macaúba palm. Additionally, these 145 markers were transferred in six other palm species resulting in transferability rates of 99% (144) in Acrocomia intumescens, 98% (143) in Acrocomia totai, 80.7% (117 EST-EST) in African oil palm (Elaeis guineensis) and peach palm (Bactris gasipaes) samples, 70% (102) in the juçara palm (Euterpe edulis) and 71.7% (104) in the hat palm (Sabal causiarum). Analysis of genetic distance showed a high separation in accordance with geographic location, establishing distinct groups by genera. Conclusions The EST markers identified in our study are a valuable resource and provide a genomic tool for genetic mapping and further genetic studies, as well as evaluation of co-location between QTLs and functionally associated markers
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the genetic structure and mating system of Acrocomia aculeata arecaceae
Genetics and Molecular Biology, 2012Co-Authors: Aluana G Abreu, Regina Helena Geribello Priolli, Joaquim Adelino Azevedofilho, Stella Maris Nucci, Ricardo Marques Coelho, Maria Imaculada Zucchi, Carlos Augusto ColomboAbstract:Acrocomia aculeata is a perennial, fruit-producing palm tree, native to tropical forests. Its fruits have spurred interest because of their significant potential for use in the cosmetic industry and as feedstock for biofuel. In the present study, the genetic structure and mating system in Acrocomia aculeata were analyzed, using eight nuclear microsatellite loci and samples from Sao Paulo and Minas Gerais states, Brazil. By means of Bayesian analysis, these populations were clustered into two or three groups. A high multilocus outcrossing rate suggests that outcrosses were predominant, although a certain degree of biparental inbreeding also occurred. Thus, although monoecious and self-compatible, there is every indication that A. aculeata bears a mixed reproductive system, with a predominance of outcrossing. Given the genetic structure revealed hereby, future conservation strategies and germplasm collecting should be focussed on sampling and preserving individuals from different clusters.
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development and characterization of microsatellites markers from the macaw
Molecular Ecology Resources, 2008Co-Authors: Stella Maris Nucci, Regina Helena Geribello Priolli, Joaquim Adelino Azevedofilho, Ricardo Marques Coelho, Carlos Augusto Colombo, T L Mata, Maria Imaculada ZucchiAbstract:Macaw (Acrocomia aculeata) is a native palm tree from tropical forests, highly abundant in Brazil and cited as one of the principal sources of plant oil, thus presenting a high potential for biodiesel production. We have optimized and utilized a set of eight polymorphic microsatellite markers for A. aculeata from an enriched genomic library. Automatic sequencing and fluorescence detection were employed to analyse 43 individuals from natural populations. In this study, we have obtained an average number of five alleles per locus. These loci will be employed in future studies of population genetics by providing subsidy information for the species conservation and genetic breeding.
Maria Ligia Rodrigues Macedo - One of the best experts on this subject based on the ideXlab platform.
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Food Value of Mealworm Grown on Acrocomia aculeata Pulp Flour
PLOS ONE, 2016Co-Authors: Ariana Vieira Alves, Adelita Maria Linzmeier, Eliana Janet Sanjinez-argandoña, Claudia Andrea Lima Cardoso, Maria Ligia Rodrigues MacedoAbstract:Insects have played an important role as human food throughout history, especially in Africa, Asia and Latin America. A good example of edible insects is the mealworm, Tenebrio molitor Linnaeus, 1758 (Coleoptera, Tenebrionidae), which are eaten in Africa, Asia, the Americas and Australia. This species is easily bred in captivity, requiring simple management. The bocaiuva (Acrocomia aculeata (Jacq.) Lodd) is an abundant palm tree found in the Brazilian Cerrado, providing fruits with high nutritional value. The aim of this work was to determine the chemical composition of T. molitor grown in different artificial diets with bocaiuva pulp flour. The nutritional composition, fatty acid composition, antioxidant activity, trypsin activity and anti-nutritional factors of larvae were analyzed. The results showed that mealworms grown on artificial diet with bocaiuva are a good source of protein (44.83%) and lipid (40.45%), with significant levels of unsaturated fatty acids (65.99%), antioxidant activity (4.5 μM Trolox/g of oil extracted from larvae) and absence of anti-nutritional factors. This study indicates a new source of biomass for growing mealworms and shows that it is possible to breed mealworms in artificial diet with bocaiuva flour without compromising the nutritional quality of the larvae.
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Composition of fatty acids in total lipids of oils extracted from Pachymerus nucleorum (Coleoptera, Chrysomelidae) larvae and Acrocomia aculeata (Arecaceae) kernels.
2016Co-Authors: Ariana Vieira Alves, Adelita Maria Linzmeier, Claudia Andrea Lima Cardoso, Eliana Janet Sanjinez Argandoña, Maria Ligia Rodrigues MacedoAbstract:Composition of fatty acids in total lipids of oils extracted from Pachymerus nucleorum (Coleoptera, Chrysomelidae) larvae and Acrocomia aculeata (Arecaceae) kernels.
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chemical and nutritional evaluation of kernels of bocaiuva Acrocomia aculeata jacq lodd
Food Science and Technology International, 2006Co-Authors: Priscila Aiko Hiane, Paulo A Baldasso, Sergio Marangoni, Maria Ligia Rodrigues MacedoAbstract:Protein characterization and results of proximate composition and mineral analyses of fruit kernels of bocaiuva, Acrocomia aculeata (Jacq.) Lodd., are reported. The kernels presented high contents of oil (51.7%), protein (17.6%) and fiber (15.8%). The seeds´ soluble proteins were isolated according to their solubility. The main separated proteins were globulins (53.5%) and glutelins (40.0%). Moreover, the presence of low molecular mass proteases in these two fractions was shown by the SDS-PAGE method. The assays of protease-inhibitory and hemagglutinating activities showed that bocaiuva´s protein fractions were not resistant to trypsin or chymotrypsin activities and that both had low lectin content. The globulin in vitro digestibility assay resembled a casein standard. Neither globulin nor glutelin enzymatic hydrolyses increased significantly (p < 0.05) after heat treatment. Threonine and lysine are the most limiting amino acids, respectively from two major protein fractions of the bocaiuva kernel, globulin (47.1% amino acid score) and glutelin (49.5% amino acid score), in terms of the theoretical profiles for children in the age range of 2 to 5 years recommended by the FAO/WHO. Bocaiuva kernels are found to be rich in calcium, phosphorus and manganese compared to some fruit nuts such as cashew and coconut.
Anderson Barbosa Evaristo - One of the best experts on this subject based on the ideXlab platform.
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variability in lignin composition and structure in cell walls of different parts of macauba Acrocomia aculeata palm fruit
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jorge Rencoret, Anderson Barbosa Evaristo, Hoon Kim, Ana Gutierrez, John Ralph, Jose C Del RioAbstract:The lignins from different anatomical parts of macauba (Acrocomia aculeata) palm fruit, namely stalks, epicarp, and endocarp, were studied. The lignin from stalks was enriched in S-lignin units (S/G 1.2) and β-ether linkages (84% of the total) and was partially acylated at the γ-OH of the lignin side-chains (26% lignin acylation), predominantly with p-hydroxybenzoates and acetates. The epicarp lignin was highly enriched in G-lignin units (S/G 0.2) and consequently depleted in β-ethers (65%) and enriched in condensed structures such as phenylcoumarans (24%) and dibenzodioxocins (3%). The endocarp lignin was strikingly different from the rest and presented large amounts of piceatannol units incorporated into the polymer. This resulted in a lignin polymer depleted in β-ethers but enriched in condensed structures and linked piceatannol moieties. The incorporation of piceatannol into the lignin polymer seems to have a role in seed protection.
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Harvest and post-harvest conditions influencing macauba (Acrocomia aculeata) oil quality attributes
Industrial Crops and Products, 2016Co-Authors: Anderson Barbosa Evaristo, José Antonio Saraiva Grossi, Samuel De Melo Goulart, Adalvan Daniel Martins, Vera Lúcia Dos Santos, Leonardo Duarte Pimentel, Sergio MotoikeAbstract:Macauba (Acrocomia aculeata), also known as macauba palm, is a good source of vegetable oil in tropical America. Its fruits are highly suitable for biodiesel production owing to the high quantity and quality of its oil. However, commercial exploitation of this species remains insufficient. Forms of harvesting, the storage period, and the use of chemicals for the postharvest preservation of macauba fruits and their effects on the associated microbiota were investigated. Mature fruits were collected only once from the mother tree and separated in two groups, a group of fruits not exposed to the ground surface (0 days) and a larger group of fruits that was placed in contact with the soil surface for 7, 14, and 21 days, to allow infestation of soil-borne microorganisms. Then the fruits were treated with 3 doses of fungicide fungicide (0, 0.2, and 0.4% v/v) and storage at room temperature during 0, 10, 20, 30 and 40 days. Hence the experiment was set in a randomized block design with four replications in a factorial Scheme 4 × 3 × 5 (period of ground surface contact, doses of fungicide and period of storage). The mesocarp oil content (OC), free fatty acids, and oxidative stability of the oil were evaluated, and the presence of microorganisms in the mesocarp and epicarp of the macauba fruits was quantified. Fungicide was found to be effective in maintaining low oil acidity of fruits unexposed to the soil surface for up to 20 days of storage and for maintaining oil oxidative stability. Regardless of the fungicide dose applied, an increase in the OC was noted for all harvest times during the first 10 days of storage. Therefore, for biodiesel production, it is recommended that the fruits should be harvested when in pre-absciscion stage (directly from the mother tree) or collected from the ground after no more than 7 days after abscission. In both cases, the fruits must be pre-treated with fungicide if storage is intended.
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POTENCIAL ENERGÉTICO DOS RESÍDUOS DO FRUTO DA MACAÚBA E SUA UTILIZAÇÃO NA PRODUÇÃO DE CARVÃO VEGETAL
Universidade Federal de Santa Maria, 2016Co-Authors: Anderson Barbosa Evaristo, Daniela Correia Martino, Adriano Henrique Ferrarez, Danilo Barros Donato, Angélica De Cássia Oliveira Carneiro, José Antonio Saraiva GrossiAbstract:The use of biomass for energy purposes has been highlighted in as a way to reduce dependence on non- renewable energy sources. Macaw palm, Acrocomia aculeata (Jacq.) Lodd. ex Martius , is an arborescent palm and has a high energy potential from the fruit processing. The aim of this study was to characterize the macaw palm residues after the fruit processing to evaluate its use “in natura” and as charcoal. The endocarp showed higher fixed carbon content, heating value and bulk density compared with other residues and lower ashes content. The heating value of endocarp charcoal was the highest, whereas the volatile matter levels of epicarp charcoal showed higher results. All evaluated residues from the macaw palm fruit processing have potential in power generation, especially the endocarp and the epicarp. The endocarp charcoal has suitable characteristics as source of energy and as a reductant
Christof B Steingass - One of the best experts on this subject based on the ideXlab platform.
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fatty acids and triacylglycerols in the mesocarp and kernel oils of maturing costa rican Acrocomia aculeata fruits
NFS Journal, 2019Co-Authors: Veronika M Lieb, Roland Schex, Victor M Jimenez, Patricia Esquivel, Reinhold Carle, Hansgeorg Schmarr, Christof B SteingassAbstract:Abstract Total lipids, fatty acids, and triacylglycerols in mesocarp and kernels from Costa Rican Acrocomia aculeata fruits were characterized at three maturity stages. C16:0 and C18:1n9 were the most abundant fatty acids in the mesocarp. C12:0, C14:0, C16:0, and C18:1n9 prevailed in the kernel oil. Comprehensive HPLC-DAD-APCI-MS and ESI-MSn analysis revealed 125 triacylglycerols with equivalent carbon numbers ranging between 28 and 52. Mesocarp oils mainly contained unsaturated triacylglycerols composed of long-chain fatty acids. In the kernel, high proportions of saturated triacylglycerols with medium-chain fatty acids were observed. Within the maturity stages assessed, fatty acid and triacylglycerol compositions remained unaffected by ripening. Total lipids in the mesocarp increased from 12.1 to 23.4% with progressing maturity, whereas those in the kernel constantly ranged between 54.1 and 56.1%. For the recovery of A. aculeata oil, utilization of all fruits in a bunch at progressed maturity is possible without affecting the composition of its major lipid fractions.
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Fatty acids and triacylglycerols in the mesocarp and kernel oils of maturing Costa Rican Acrocomia aculeata fruits
Elsevier, 2019Co-Authors: Veronika M Lieb, Roland Schex, Victor M Jimenez, Patricia Esquivel, Reinhold Carle, Hansgeorg Schmarr, Christof B SteingassAbstract:Total lipids, fatty acids, and triacylglycerols in mesocarp and kernels from Costa Rican Acrocomia aculeata fruits were characterized at three maturity stages. C16:0 and C18:1n9 were the most abundant fatty acids in the mesocarp. C12:0, C14:0, C16:0, and C18:1n9 prevailed in the kernel oil. Comprehensive HPLC-DAD-APCI-MS and ESI-MSn analysis revealed 125 triacylglycerols with equivalent carbon numbers ranging between 28 and 52. Mesocarp oils mainly contained unsaturated triacylglycerols composed of long-chain fatty acids. In the kernel, high proportions of saturated triacylglycerols with medium-chain fatty acids were observed. Within the maturity stages assessed, fatty acid and triacylglycerol compositions remained unaffected by ripening. Total lipids in the mesocarp increased from 12.1 to 23.4% with progressing maturity, whereas those in the kernel constantly ranged between 54.1 and 56.1%. For the recovery of A. aculeata oil, utilization of all fruits in a bunch at progressed maturity is possible without affecting the composition of its major lipid fractions. Keywords: Total lipids, Macauba, Fruit ripening, Fatty acids, Triacylglycerols, HPLC linear retention indice
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hplc dad apci esi msn analysis of carotenoids and α tocopherol in costa rican Acrocomia aculeata fruits of varying maturity stages
Food Research International, 2018Co-Authors: Roland Schex, Veronika M Lieb, Victor M Jimenez, Patricia Esquivel, Ralf M Schweiggert, Reinhold Carle, Christof B SteingassAbstract:Carotenoids and tocopherols were characterised in the meso- and exocarp of wild-growing Costa Rican Acrocomia aculeata fruits. Comprehensive profiling of these lipophilic micronutrients in fruits of three varying maturity stages was conducted for the first time. A method for the simultaneous extraction and quantitation of carotenoids and α-tocopherol was developed and validated. Detailed HPLC-DAD-APCI/ESI-MSn analyses enabled the identification of α-tocopherol and 25 carotenoids. The latter comprised antheraxanthin, β-carotene, lutein, luteoxanthin, neoxanthin, phytoene, phytofluene, violaxanthin, zeaxanthin, and several (Z)-isomers of the aforementioned compounds. Quantitation by HPLC-DAD/FLD revealed total carotenoid concentrations of 872±178 and 3075±407μg/100g fresh weight in the meso- and exocarp of fully ripe fruits, respectively. In both fruit fractions, progressing maturation resulted in the accumulation of phytoene, phytofluene, (all-E)-zeaxanthin, (all-E)-antheraxanthin, and (all-E)-violaxanthin. Carotenoid profiling was supported by multivariate data analysis. Carotenoid precursors and xanthophyll cycle pigments characterised Macauba fruits of full maturity.
Sergio Yoshimitsu Motoike - One of the best experts on this subject based on the ideXlab platform.
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enzyme activity and reserve mobilization during macaw palm Acrocomia aculeata seed germination
Acta Botanica Brasilica, 2016Co-Authors: Elisa Monteze Bicalho, Sergio Yoshimitsu Motoike, Eduardo Euclydes De Lima E Borges, Glauciana Da Mata Ataide, Valeria Monteze GuimaraesAbstract:Reserve mobilization in seeds occurs after visible germination, which is marked by the protrusion of the radicle or cotyledonary petiole, as in species of Arecaceae. Acrocomia aculeata (macaw palm), usually produces hard seeds whose endosperm has mannan-rich cell walls. We investigated the composition of storage compounds in macaw palm seed and the roles of two enzymes (endo-β-mannanase, α-galactosidase) during and after germination. The seeds were firstly submitted to pre-established protocol to overcome dormancy and promote germination. Enzyme activity in both embryo and endosperm were assayed from the initiation of germinative activities until leaf sheath appearance, and the status of seed structures and reserve compounds were evaluated. Protein content of the embryo decreased with the initiation of imbibition while the lipid content began decreasing six days after removal of the operculum. Increases in enzyme activity and starch content were both observed after visible germination. We suggest that endo-β-mannanase and α-galactosidase become active immediately at germination, facilitating haustorium expansion and providing carbohydrates for initial seedling development. Protein is the first storage compound mobilized during early imbibition, and the observed increase in the starch content of the haustorium was related to lipid degradation in that organ and mannan degradation in the adjacent endosperm.
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Recording and damage characterization of coleopters in macao palm
Embrapa Florestas, 2015Co-Authors: Sebastián Giraldo Montoya, Sergio Yoshimitsu Motoike, Kacilda Naomi Kuki, Cleber Macedo De Oliveira, Isabela Cristina Gomes HonorioAbstract:The objective of this work was to identify the species of beetles present in a macaw palm (Acrocomia aculeata (Jacq.) Lodd) native population as well as describe their activities, for further study of plant- insect interaction. Adult fruit weevils and brown beetles were collected manually in the fruits and the leaflets of adult plants of macaw palms. The insects were associated with the premature fruits abortion and with the reduction of the photosynthetic area of the plants
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anatomy histochemistry and ultrastructure of seed and somatic embryo of Acrocomia aculeata arecaceae
Scientia Agricola, 2010Co-Authors: Elisa Ferreira Moura, Marilia Contin Ventrella, Sergio Yoshimitsu MotoikeAbstract:Macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Mart.) is a tropical species with multiple uses, including oil supply for biodiesel production. However, structural and physiological studies of the seed are still scarce, as well as in vitro propagation techniques. The aim of this study was to characterize the anatomy, histochemistry and ultrastructure of the seed and the in vitro somatic embryo of A. aculeata, gaining insight into relationships between the post-harvest seed behavior and the somatic embryo conversion to plant. The zygotic embryo and the endosperm show high quantities of protein and lipids, stored in protein and lipid bodies, respectively. The cell wall storage polysaccharides occur in the thickened cell wall of the endosperm. The absence of vacuoles and the few organelles, except for the storage ones, indicate low metabolic state of the zygotic embryo, which suggests a orthodox behavior of the seed at maturity. The somatic embryo shows a shoot meristem with few leaf primordia, vacuolated cells and, occasionally, amyloplasts , but not lipid or protein reserves. These characteristics, common in somatic embryogenesis, could be associated with the maturation phase, and, consequently, with the low conversion of these embryos into plants.
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somatic embryogenesis in macaw palm Acrocomia aculeata from zygotic embryos
Scientia Horticulturae, 2009Co-Authors: Elisa Ferreira Moura, Sergio Yoshimitsu Motoike, Marilia Contin Ventrella, Mychelle CarvalhoAbstract:Abstract Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination.
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histological study of somatic embryogenesis induction on zygotic embryos of macaw palm Acrocomia aculeata jacq lodd ex martius
Plant Cell Tissue and Organ Culture, 2008Co-Authors: Elisa Ferreira Moura, Sergio Yoshimitsu Motoike, Marilia Contin Ventrella, Mychelle Carvalho, Candida Elisa ManfioAbstract:The aim of this paper was to describe the histological events that led to somatic embryogenesis in macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius). Zygotic embryos were inoculated on Y3 medium containing 9 μM 4-amino-3,5,6-trichloropicolonic acid (picloram). Somatic embryos regenerated from nodular callus on induction medium with activated charcoal under photoperiod or without activated charcoal under dark. Many proembryos originated from the fundamental meristem after 10–20 days of culture. When transferred to medium containing activated charcoal, under photoperiod, calli regenerated into somatic embryos of unicellular origin. These embryos had protoderm, plumule and procambial strands and some of them could germinate. After 30–40 days of culture, meristematic masses grew from procambial cells. The masses generated nodular callus, and after transfer to medium without activated charcoal, under dark, they generated somatic embryos of multicellular origin. Those embryos did not regenerate into plants.
