The Experts below are selected from a list of 594 Experts worldwide ranked by ideXlab platform
Kirstine Klitgaard - One of the best experts on this subject based on the ideXlab platform.
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Revisiting bovine pyometra—New insights into the disease using a culture-independent deep sequencing approach
Veterinary Microbiology, 2014Co-Authors: Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Jørgen S. Agerholm, Tim Kåre Jensen, Kirstine KlitgaardAbstract:The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing. We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.
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Revisiting bovine pyometra--new insights into the disease using a culture-independent deep sequencing approach.
Veterinary microbiology, 2014Co-Authors: Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Jørgen S. Agerholm, Tim Kåre Jensen, Kirstine KlitgaardAbstract:The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing. We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.
Chris Detter - One of the best experts on this subject based on the ideXlab platform.
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Standards in Genomic Sciences (2010) 3:126-135 DOI:10.4056/sigs.1123072 The Genomic Standards Consortium Complete genome sequence of Arcanobacterium haemolyticum type strain (11018T)
2015Co-Authors: Montri Yasawong, Hazuki Teshima, Alla Lapidus, Matt Nolan, Susan Lucas, Glavina Del Rio, Hope Tice, Jan-fang Cheng, David Bruce, Chris DetterAbstract:cies of the genus Arcanobacterium, which belongs to the family Actinomycetaceae. The strain is of interest because it is an obligate parasite of the pharynx of humans and farm ani-mal; occasionally, it causes pharyngeal or skin lesions. It is a Gram-positive, nonmotile and non-sporulating bacterium. The strain described in this study was isolated from infections amongst American soldiers of certain islands of the North and West Pacific. This is the first completed sequence of a member of the genus Arcanobacterium and the ninth type strain genome from the family Actinomycetaceae. The 1,986,154 bp long genome with its 1,821 protein-coding and 64 RNA genes is a part of the Genomic Encyclopedia of Bacteria an
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Complete genome sequence of Arcanobacterium haemolyticum type strain (11018 T )
Standards in genomic sciences, 2010Co-Authors: Montri Yasawong, Hazuki Teshima, Alla Lapidus, Matt Nolan, Susan Lucas, Hope Tice, Jan-fang Cheng, David Bruce, Tijana Glavina Del Rio, Chris DetterAbstract:Arcanobacterium haemolyticum (ex MacLean et al. 1946) Collins et al. 1983 is the type species of the genus Arcanobacterium, which belongs to the family Actinomycetaceae. The strain is of interest because it is an obligate parasite of the pharynx of humans and farm animal; occasionally, it causes pharyngeal or skin lesions. It is a Gram-positive, nonmotile and non-sporulating bacterium. The strain described in this study was isolated from infections amongst American soldiers of certain islands of the North and West Pacific. This is the first completed sequence of a member of the genus Arcanobacterium and the ninth type strain genome from the family Actinomycetaceae. The 1,986,154 bp long genome with its 1,821 protein-coding and 64 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
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Complete genome sequence of Arcanobacterium haemolyticum type strain (11018^T)
Standards in Genomic Sciences, 2010Co-Authors: Montri Yasawong, Hazuki Teshima, Alla Lapidus, Matt Nolan, Susan Lucas, Hope Tice, Jan-fang Cheng, David Bruce, Tijana Glavina Del Rio, Chris DetterAbstract:Arcanobacterium haemolyticum (ex MacLean et al. 1946) Collins et al. 1983 is the type species of the genus Arcanobacterium , which belongs to the family Actinomycetaceae . The strain is of interest because it is an obligate parasite of the pharynx of humans and farm animal; occasionally, it causes pharyngeal or skin lesions. It is a Gram-positive, nonmotile and non-sporulating bacterium. The strain described in this study was isolated from infections amongst American soldiers of certain islands of the North and West Pacific. This is the first completed sequence of a member of the genus Arcanobacterium and the ninth type strain genome from the family Actinomycetaceae . The 1,986,154 bp long genome with its 1,821 protein-coding and 64 RNA genes is a part of the G enomic E ncyclopedia of B acteria and A rchaea project.
Lif Rødtness Vesterby Knudsen - One of the best experts on this subject based on the ideXlab platform.
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Revisiting bovine pyometra—New insights into the disease using a culture-independent deep sequencing approach
Veterinary Microbiology, 2014Co-Authors: Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Jørgen S. Agerholm, Tim Kåre Jensen, Kirstine KlitgaardAbstract:The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing. We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.
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Revisiting bovine pyometra--new insights into the disease using a culture-independent deep sequencing approach.
Veterinary microbiology, 2014Co-Authors: Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Jørgen S. Agerholm, Tim Kåre Jensen, Kirstine KlitgaardAbstract:The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing. We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.
Guan-jun Chen - One of the best experts on this subject based on the ideXlab platform.
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Flaviflexus huanghaiensis gen. nov., sp. nov., an actinobacterium of the family Actinomycetaceae
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Ting-ting Miao, Xue-zheng Lin, Qian-qian Liu, Guan-jun ChenAbstract:Strain H5T was isolated from a sediment sample collected from the coastal area of Qingdao, China. The cells were Gram-stain-positive, non-motile, straight or curved rods. The temperature range for growth was 20–37 °C and the pH for growth ranged from 6.5 to 9.0, with optimum growth occurring in the temperature range 28–30 °C and pH range 7.5–8.0. Growth occurred in the presence of 0–6 % (w/v) NaCl (optimum, 0–2 %). Strain H5T had MK-9, MK-9(H2) and MK-9(H4) as the major menaquinones and C18 : 1ω9c, C16 : 0, C14 : 0, C18 : 0 and C16 : 1ω9c as major fatty acids. The cell-wall peptidoglycan type was A5α l-Lys-l-Ala-l-Lys-d-Glu. The major polar lipids were phosphatidylglycerol (PG), an unknown phospholipid (PL1) and two unknown phosphoglycolipids (PGL1, PGL2). An unknown phospholipid (PL2) and two unknown glycolipids (GL1, GL2) were present in moderate to minor amounts in the polar lipid profile. The genomic DNA G+C content was 61.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H5T represents a novel lineage in the family Actinomycetaceae . On the basis of phenotypic, physiological and molecular characteristics, it is proposed that the novel isolate should be classified as a novel species in a new genus: Flaviflexus huanghaiensis gen. nov., sp. nov., with strain H5T ( = DSM 24315T = CICC 10486T) as the type strain of the type species.
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Flaviflexus huanghaiensis gen. nov., sp. nov., an actinobacterium of the family Actinomycetaceae.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Ting-ting Miao, Xue-zheng Lin, Qian-qian Liu, Guan-jun ChenAbstract:Strain H5T was isolated from a sediment sample collected from the coastal area of Qingdao, China. The cells were Gram-stain-positive, non-motile, straight or curved rods. The temperature range for growth was 20–37 °C and the pH for growth ranged from 6.5 to 9.0, with optimum growth occurring in the temperature range 28–30 °C and pH range 7.5–8.0. Growth occurred in the presence of 0–6 % (w/v) NaCl (optimum, 0–2 %). Strain H5T had MK-9, MK-9(H2) and MK-9(H4) as the major menaquinones and C18 : 1ω9c, C16 : 0, C14 : 0, C18 : 0 and C16 : 1ω9c as major fatty acids. The cell-wall peptidoglycan type was A5α l-Lys-l-Ala-l-Lys-d-Glu. The major polar lipids were phosphatidylglycerol (PG), an unknown phospholipid (PL1) and two unknown phosphoglycolipids (PGL1, PGL2). An unknown phospholipid (PL2) and two unknown glycolipids (GL1, GL2) were present in moderate to minor amounts in the polar lipid profile. The genomic DNA G+C content was 61.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H5T represents a novel lineage in the family Actinomycetaceae . On the basis of phenotypic, physiological and molecular characteristics, it is proposed that the novel isolate should be classified as a novel species in a new genus: Flaviflexus huanghaiensis gen. nov., sp. nov., with strain H5T ( = DSM 24315T = CICC 10486T) as the type strain of the type species.
Alain Lozniewski - One of the best experts on this subject based on the ideXlab platform.
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Comparison of Vitek MS and MALDI Biotyper for Identification of Actinomycetaceae of Clinical Importance.
Journal of clinical microbiology, 2015Co-Authors: Janina Ferrand, Hélène Hochard, Victoria Girard, Nejla Aissa, Baptiste Bogard, Corentine Alauzet, Alain LozniewskiAbstract:The Vitek MS in vitro diagnostic (IVD) and MALDI Biotyper IVD systems were evaluated for the identification of 158 strains of Actinomycetaceae. Correct species-level identification rates of 60.7% and 58.2% were obtained with the Vitek MS system after direct deposit and with the MALDI Biotyper system after on-plate formic acid treatment, respectively.