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Actinomycin

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Weidong Xie – One of the best experts on this subject based on the ideXlab platform.

  • Actinomycin v inhibits migration and invasion via suppressing snail slug mediated epithelial mesenchymal transition progression in human breast cancer mda mb 231 cells in vitro
    Marine Drugs, 2019
    Co-Authors: Shiqi Lin, Caiyun Zhang, Fangyuan Liu, Fujuan Jia, Zhuo Han, Weidong Xie

    Abstract:

    Actinomycin V, an analog of Actinomycin D produced by the marine-derived actinomycete Streptomyces sp., possessing a 4-ketoproline instead of a 4-proline in Actinomycin D. In this study, the involvement of snail/slug-mediated epithelial-mesenchymal transition (EMT) in the anti-migration and -invasion actions of Actinomycin V was investigated in human breast cancer MDA-MB-231 cells in vitro. Cell proliferation effect was evaluated by 3-(4,5-Dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. Wound-healing and Transwell assay were performed to investigate the anti-migration and -invasion effects of Actinomycin V. Western blotting was used to detect the expression levels of E-cadherin, N-cadherin, vimentin, snail, slug, zinc finger E-box binding homeobox 1 (ZEB1), and twist proteins and the mRNA levels were detected by rt-PCR. Actinomycin V showed stronger cytotoxic activity than that of Actinomycin D. Actinomycin V up-regulated both of the protein and mRNA expression levels of E-cadherin and down-regulated that of N-cadherin and vimentin in the same cells. In this connection, Actinomycin V decreased the snail and slug protein expression, and consequently inhibited cells EMT procession. Our results suggest that Actinomycin V inhibits EMT-mediated migration and invasion via decreasing snail and slug expression, which exhibits therapeutic potential for the treatment of breast cancer and further toxicity investigation in vivo is needed.

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  • Actinomycin V Inhibits Migration and Invasion via Suppressing Snail/Slug-Mediated Epithelial-Mesenchymal Transition Progression in Human Breast Cancer MDA-MB-231 Cells In Vitro
    MDPI AG, 2019
    Co-Authors: Shiqi Lin, Caiyun Zhang, Fangyuan Liu, Fujuan Jia, Zhuo Han, Weidong Xie

    Abstract:

    Actinomycin V, an analog of Actinomycin D produced by the marine-derived actinomycete Streptomyces sp., possessing a 4-ketoproline instead of a 4-proline in Actinomycin D. In this study, the involvement of snail/slug-mediated epithelial-mesenchymal transition (EMT) in the anti-migration and -invasion actions of Actinomycin V was investigated in human breast cancer MDA-MB-231 cells in vitro. Cell proliferation effect was evaluated by 3-(4,5-Dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. Wound-healing and Transwell assay were performed to investigate the anti-migration and -invasion effects of Actinomycin V. Western blotting was used to detect the expression levels of E-cadherin, N-cadherin, vimentin, snail, slug, zinc finger E-box binding homeobox 1 (ZEB1), and twist proteins and the mRNA levels were detected by rt-PCR. Actinomycin V showed stronger cytotoxic activity than that of Actinomycin D. Actinomycin V up-regulated both of the protein and mRNA expression levels of E-cadherin and down-regulated that of N-cadherin and vimentin in the same cells. In this connection, Actinomycin V decreased the snail and slug protein expression, and consequently inhibited cells EMT procession. Our results suggest that Actinomycin V inhibits EMT-mediated migration and invasion via decreasing snail and slug expression, which exhibits therapeutic potential for the treatment of breast cancer and further toxicity investigation in vivo is needed

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Shiqi Lin – One of the best experts on this subject based on the ideXlab platform.

  • Actinomycin v inhibits migration and invasion via suppressing snail slug mediated epithelial mesenchymal transition progression in human breast cancer mda mb 231 cells in vitro
    Marine Drugs, 2019
    Co-Authors: Shiqi Lin, Caiyun Zhang, Fangyuan Liu, Fujuan Jia, Zhuo Han, Weidong Xie

    Abstract:

    Actinomycin V, an analog of Actinomycin D produced by the marine-derived actinomycete Streptomyces sp., possessing a 4-ketoproline instead of a 4-proline in Actinomycin D. In this study, the involvement of snail/slug-mediated epithelial-mesenchymal transition (EMT) in the anti-migration and -invasion actions of Actinomycin V was investigated in human breast cancer MDA-MB-231 cells in vitro. Cell proliferation effect was evaluated by 3-(4,5-Dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. Wound-healing and Transwell assay were performed to investigate the anti-migration and -invasion effects of Actinomycin V. Western blotting was used to detect the expression levels of E-cadherin, N-cadherin, vimentin, snail, slug, zinc finger E-box binding homeobox 1 (ZEB1), and twist proteins and the mRNA levels were detected by rt-PCR. Actinomycin V showed stronger cytotoxic activity than that of Actinomycin D. Actinomycin V up-regulated both of the protein and mRNA expression levels of E-cadherin and down-regulated that of N-cadherin and vimentin in the same cells. In this connection, Actinomycin V decreased the snail and slug protein expression, and consequently inhibited cells EMT procession. Our results suggest that Actinomycin V inhibits EMT-mediated migration and invasion via decreasing snail and slug expression, which exhibits therapeutic potential for the treatment of breast cancer and further toxicity investigation in vivo is needed.

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  • Actinomycin V Inhibits Migration and Invasion via Suppressing Snail/Slug-Mediated Epithelial-Mesenchymal Transition Progression in Human Breast Cancer MDA-MB-231 Cells In Vitro
    MDPI AG, 2019
    Co-Authors: Shiqi Lin, Caiyun Zhang, Fangyuan Liu, Fujuan Jia, Zhuo Han, Weidong Xie

    Abstract:

    Actinomycin V, an analog of Actinomycin D produced by the marine-derived actinomycete Streptomyces sp., possessing a 4-ketoproline instead of a 4-proline in Actinomycin D. In this study, the involvement of snail/slug-mediated epithelial-mesenchymal transition (EMT) in the anti-migration and -invasion actions of Actinomycin V was investigated in human breast cancer MDA-MB-231 cells in vitro. Cell proliferation effect was evaluated by 3-(4,5-Dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. Wound-healing and Transwell assay were performed to investigate the anti-migration and -invasion effects of Actinomycin V. Western blotting was used to detect the expression levels of E-cadherin, N-cadherin, vimentin, snail, slug, zinc finger E-box binding homeobox 1 (ZEB1), and twist proteins and the mRNA levels were detected by rt-PCR. Actinomycin V showed stronger cytotoxic activity than that of Actinomycin D. Actinomycin V up-regulated both of the protein and mRNA expression levels of E-cadherin and down-regulated that of N-cadherin and vimentin in the same cells. In this connection, Actinomycin V decreased the snail and slug protein expression, and consequently inhibited cells EMT procession. Our results suggest that Actinomycin V inhibits EMT-mediated migration and invasion via decreasing snail and slug expression, which exhibits therapeutic potential for the treatment of breast cancer and further toxicity investigation in vivo is needed

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Zhiqiang Xiong – One of the best experts on this subject based on the ideXlab platform.

  • optimization of medium composition for Actinomycin x2 production by streptomyces spp jau4234 using response surface methodology
    Journal of Industrial Microbiology & Biotechnology, 2008
    Co-Authors: Zhiqiang Xiong

    Abstract:

    The effects of cultivation medium compositions including soybean meal, peptone, soybean oil and cornstarch for Actinomycin X2 production by Streptomyces spp JAU4234 were accessed by using response surface methodology. The 24 full factorial designs and the paths of steepest ascent were effective in searching for the major factors of Actinomycin X2 production. In this study, cornstarch and soybean oil showed negative effect on Actinomycin X2 production based on the first-order regression coefficients derived from MINITAB software. Subsequently, a central composite design for optimization was further investigated. Preliminary studies showed that soybean meal and peptone were believed to be the major factors for Actinomycin X2 production. Estimated optimum compositions for the production of actionmycin X2 were as follows (g/l): soybean meal 21.65 and peptone 9.41, and result in a maximum actionmycin X2 production of 617.4 mg/l. This value was closed to the 612 mg/l actionmycin X2 production from actual experimental observations. The yield of actionmycin X2 was increased by 36.9% by culturing the strain Streptomyces spp JAU4234 in the nutritionally optimized fermentation medium.

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