The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Mar Villamiel - One of the best experts on this subject based on the ideXlab platform.
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effect of purification of galactooligosaccharides derived from lactulose with saccharomyces cerevisiae on their capacity to bind immune cell receptor dectin 2
Food Research International, 2019Co-Authors: Ian D Young, Alexandra Wittmann, Antonia Montilla, Agustin Olano, Norihito Kawasaki, Mar VillamielAbstract:Abstract Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) beneficial for human health including immunomodulatory properties; however, the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification introduces yeast-derived proteins reActive to Dectin-2, an innate immune receptor for fungal polysaccharides. Using a cell-based bioassay, we tested the binding of OsLu and GOS samples to Dectin-2. While OsLu purified with Active Charcoal and commercial GOS failed to bind to Dectin-2, we found OsLu-Sc bound to this receptor. The carbohydrate-binding incompetent mutant of Dectin-2 failed to bind to OsLu-Sc. These data suggest that OsLu-Sc introduced carbohydrate ligands for Dectin-2. In accordance with this, proteomic analysis revealed OsLu-Sc contained S. cerevisiae-derived mannoproteins. Therefore, our data highlight the importance of the purification method for OsLu, which may positively affect the bioactivity of OsLu. Data are available via ProteomeXchange with identifier PXD010495.
Daniel Schweich - One of the best experts on this subject based on the ideXlab platform.
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Adsorption of complex phenolic compounds on Active Charcoal: breakthrough curves.
Chemical Engineering Journal, 2010Co-Authors: Dominique Richard, Maria De Lourdes Delgado Nunez, Daniel SchweichAbstract:Abstract The transient adsorption of catechol from aqueous solution ( C 0 = 5 kg/m3) on activated carbon in an upflow fixed-bed column at 293 K was studied. The critical time at which the early breakthrough of the maximum admissible concentration ( C crit = 0.3 × 1 0 − 3 kg/m3) occurs is deduced from a Homogeneous Surface Diffusion Model (HSDM) that accounts for adsorption equilibrium and mass-transfer kinetics. The mass-transfer coefficient is measured using a thin bed adsorption method and a correlation is proposed to account for its dependence with the flow rate. The sensitivity of the model for the prediction of the critical time to the different parameters is discussed and it is found to be mostly dependant upon the mass-transfer coefficient K f and the adsorbant mean particle diameter d P . In addition, the critical time has been proved to increase with the adsorption capacity q max . The existence of an optimal flow of polluted effluent through the column to achieve the removal of the pollutant with the highest efficiency is observed.
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adsorption of complex phenolic compounds on Active Charcoal adsorption capacity and isotherms
Chemical Engineering Journal, 2009Co-Authors: Dominique Richard, Maria De Lourdes Delgado Nunez, Daniel SchweichAbstract:Adsorption of polyfunctional phenols encountered in olive oil mill waste waters was carried out on an Active Charcoal at 293 K. The relation between the structure of the phenol and its adsorption capacity was discussed. Different isotherm equations were considered for modelling the experimental data and their parameters and standard deviations were determined. Freundlich equation was found to provide the best fit except for the catechol.
Ian D Young - One of the best experts on this subject based on the ideXlab platform.
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effect of purification of galactooligosaccharides derived from lactulose with saccharomyces cerevisiae on their capacity to bind immune cell receptor dectin 2
Food Research International, 2019Co-Authors: Ian D Young, Alexandra Wittmann, Antonia Montilla, Agustin Olano, Norihito Kawasaki, Mar VillamielAbstract:Abstract Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) beneficial for human health including immunomodulatory properties; however, the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification introduces yeast-derived proteins reActive to Dectin-2, an innate immune receptor for fungal polysaccharides. Using a cell-based bioassay, we tested the binding of OsLu and GOS samples to Dectin-2. While OsLu purified with Active Charcoal and commercial GOS failed to bind to Dectin-2, we found OsLu-Sc bound to this receptor. The carbohydrate-binding incompetent mutant of Dectin-2 failed to bind to OsLu-Sc. These data suggest that OsLu-Sc introduced carbohydrate ligands for Dectin-2. In accordance with this, proteomic analysis revealed OsLu-Sc contained S. cerevisiae-derived mannoproteins. Therefore, our data highlight the importance of the purification method for OsLu, which may positively affect the bioactivity of OsLu. Data are available via ProteomeXchange with identifier PXD010495.
Maria De Lourdes Delgado Nunez - One of the best experts on this subject based on the ideXlab platform.
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Adsorption of complex phenolic compounds on Active Charcoal: breakthrough curves.
Chemical Engineering Journal, 2010Co-Authors: Dominique Richard, Maria De Lourdes Delgado Nunez, Daniel SchweichAbstract:Abstract The transient adsorption of catechol from aqueous solution ( C 0 = 5 kg/m3) on activated carbon in an upflow fixed-bed column at 293 K was studied. The critical time at which the early breakthrough of the maximum admissible concentration ( C crit = 0.3 × 1 0 − 3 kg/m3) occurs is deduced from a Homogeneous Surface Diffusion Model (HSDM) that accounts for adsorption equilibrium and mass-transfer kinetics. The mass-transfer coefficient is measured using a thin bed adsorption method and a correlation is proposed to account for its dependence with the flow rate. The sensitivity of the model for the prediction of the critical time to the different parameters is discussed and it is found to be mostly dependant upon the mass-transfer coefficient K f and the adsorbant mean particle diameter d P . In addition, the critical time has been proved to increase with the adsorption capacity q max . The existence of an optimal flow of polluted effluent through the column to achieve the removal of the pollutant with the highest efficiency is observed.
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adsorption of complex phenolic compounds on Active Charcoal adsorption capacity and isotherms
Chemical Engineering Journal, 2009Co-Authors: Dominique Richard, Maria De Lourdes Delgado Nunez, Daniel SchweichAbstract:Adsorption of polyfunctional phenols encountered in olive oil mill waste waters was carried out on an Active Charcoal at 293 K. The relation between the structure of the phenol and its adsorption capacity was discussed. Different isotherm equations were considered for modelling the experimental data and their parameters and standard deviations were determined. Freundlich equation was found to provide the best fit except for the catechol.
Dominique Richard - One of the best experts on this subject based on the ideXlab platform.
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Adsorption of complex phenolic compounds on Active Charcoal: breakthrough curves.
Chemical Engineering Journal, 2010Co-Authors: Dominique Richard, Maria De Lourdes Delgado Nunez, Daniel SchweichAbstract:Abstract The transient adsorption of catechol from aqueous solution ( C 0 = 5 kg/m3) on activated carbon in an upflow fixed-bed column at 293 K was studied. The critical time at which the early breakthrough of the maximum admissible concentration ( C crit = 0.3 × 1 0 − 3 kg/m3) occurs is deduced from a Homogeneous Surface Diffusion Model (HSDM) that accounts for adsorption equilibrium and mass-transfer kinetics. The mass-transfer coefficient is measured using a thin bed adsorption method and a correlation is proposed to account for its dependence with the flow rate. The sensitivity of the model for the prediction of the critical time to the different parameters is discussed and it is found to be mostly dependant upon the mass-transfer coefficient K f and the adsorbant mean particle diameter d P . In addition, the critical time has been proved to increase with the adsorption capacity q max . The existence of an optimal flow of polluted effluent through the column to achieve the removal of the pollutant with the highest efficiency is observed.
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adsorption of complex phenolic compounds on Active Charcoal adsorption capacity and isotherms
Chemical Engineering Journal, 2009Co-Authors: Dominique Richard, Maria De Lourdes Delgado Nunez, Daniel SchweichAbstract:Adsorption of polyfunctional phenols encountered in olive oil mill waste waters was carried out on an Active Charcoal at 293 K. The relation between the structure of the phenol and its adsorption capacity was discussed. Different isotherm equations were considered for modelling the experimental data and their parameters and standard deviations were determined. Freundlich equation was found to provide the best fit except for the catechol.
