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Aristidis Tsatsakis - One of the best experts on this subject based on the ideXlab platform.
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amphiphilic poly n vinylpyrrolidone nanoparticles as carriers for non steroidal anti inflammatory drugs in vitro cytoToxicity and in vivo Acute Toxicity Study
Nanomedicine: Nanotechnology Biology and Medicine, 2017Co-Authors: A N Kuskov, Pavel P Kulikov, Mikhail I Shtilman, Anastasia V Goryachaya, Manolis Tzatzarakis, Anca Oana Docea, Kelly Velonia, Aristidis TsatsakisAbstract:Polymeric nanoparticles were prepared from self-assembled amphiphilic N-vinylpyrrolidone polymers in aqueous media and evaluated as novel carriers of indomethacin, a non-steroidal, anti-inflammatory drug. It was determined that these nanoparticles could be created in spherical morphologies with sizes less than 100nm, narrow size distributions and high indomethacin contents(up to 35%) combined with high drug loading efficiencies(up to 95%). In cytoToxicity tests using the human embryonic stem cell derived fibroblasts (EBF-H9) and hepatocellular carcinoma cells (HepG2), the indomethacin-loaded polymeric nanoparticles showed higher cell viability compared to that of free indomethacin at the same concentration. The median LD50 values, determined by the Litchfield-Wilcoxon method, were 55-70mg/kg body weight depending on the polymer molecular design in both mice and rats. Based on the acquired results, these novel amphiphilic poly-N-vinylpyrrolidone nanoparticles can be considered as potential carriers for new, highly efficient, injectable drug delivery systems for hydrophobic drugs such as indomethacin.
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amphiphilic poly n vynilpyrrolidone nanoparticles cytoToxicity and Acute Toxicity Study
Food and Chemical Toxicology, 2016Co-Authors: A N Kuskov, Pavel P Kulikov, Mikhail I Shtilman, Valerii N Rakitskii, Aristidis TsatsakisAbstract:The aim of the present Study was to evaluate the cytoToxicity against MCF-7 cells and Acute intraperitoneal Toxicity of amphiphilic poly-N-vinylpyrrolidone nanoparticles to confirm possibility of their application for creation of novel drug delivery systems. The effect of cellular uptake of polymeric nanoparticles on human cancer cell line MCF-7 cells was investigated by MTT assay. MTT analysis showed that tested amphiphilic polymers were essentially non-toxic. In Acute Toxicity studies, LD50 and other Toxicity indexes were evaluated, under which no deaths or treatment related complications were observed even in high concentration treatment for 14 days of experiment. For histological analysis, organs of the animals were weighed and examined. No animal died during the Study and no significant changes have been observed regarding body weight, feed consumption, organ weight or histological data. Obtained results show that amphiphilic poly-N-vinylpyrrolidone nanoparticles possessed no Toxicity against cells and in animals after intraperitoneal administration. Thus, amphiphilic PVP nanoparticles demonstrate high potential as carriers for novel high-effective drug delivery systems.
A N Kuskov - One of the best experts on this subject based on the ideXlab platform.
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amphiphilic poly n vinylpyrrolidone nanoparticles as carriers for non steroidal anti inflammatory drugs in vitro cytoToxicity and in vivo Acute Toxicity Study
Nanomedicine: Nanotechnology Biology and Medicine, 2017Co-Authors: A N Kuskov, Pavel P Kulikov, Mikhail I Shtilman, Anastasia V Goryachaya, Manolis Tzatzarakis, Anca Oana Docea, Kelly Velonia, Aristidis TsatsakisAbstract:Polymeric nanoparticles were prepared from self-assembled amphiphilic N-vinylpyrrolidone polymers in aqueous media and evaluated as novel carriers of indomethacin, a non-steroidal, anti-inflammatory drug. It was determined that these nanoparticles could be created in spherical morphologies with sizes less than 100nm, narrow size distributions and high indomethacin contents(up to 35%) combined with high drug loading efficiencies(up to 95%). In cytoToxicity tests using the human embryonic stem cell derived fibroblasts (EBF-H9) and hepatocellular carcinoma cells (HepG2), the indomethacin-loaded polymeric nanoparticles showed higher cell viability compared to that of free indomethacin at the same concentration. The median LD50 values, determined by the Litchfield-Wilcoxon method, were 55-70mg/kg body weight depending on the polymer molecular design in both mice and rats. Based on the acquired results, these novel amphiphilic poly-N-vinylpyrrolidone nanoparticles can be considered as potential carriers for new, highly efficient, injectable drug delivery systems for hydrophobic drugs such as indomethacin.
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amphiphilic poly n vynilpyrrolidone nanoparticles cytoToxicity and Acute Toxicity Study
Food and Chemical Toxicology, 2016Co-Authors: A N Kuskov, Pavel P Kulikov, Mikhail I Shtilman, Valerii N Rakitskii, Aristidis TsatsakisAbstract:The aim of the present Study was to evaluate the cytoToxicity against MCF-7 cells and Acute intraperitoneal Toxicity of amphiphilic poly-N-vinylpyrrolidone nanoparticles to confirm possibility of their application for creation of novel drug delivery systems. The effect of cellular uptake of polymeric nanoparticles on human cancer cell line MCF-7 cells was investigated by MTT assay. MTT analysis showed that tested amphiphilic polymers were essentially non-toxic. In Acute Toxicity studies, LD50 and other Toxicity indexes were evaluated, under which no deaths or treatment related complications were observed even in high concentration treatment for 14 days of experiment. For histological analysis, organs of the animals were weighed and examined. No animal died during the Study and no significant changes have been observed regarding body weight, feed consumption, organ weight or histological data. Obtained results show that amphiphilic poly-N-vinylpyrrolidone nanoparticles possessed no Toxicity against cells and in animals after intraperitoneal administration. Thus, amphiphilic PVP nanoparticles demonstrate high potential as carriers for novel high-effective drug delivery systems.
A A Ebbo - One of the best experts on this subject based on the ideXlab platform.
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Acute and sub chronic Toxicity evaluation of the crude methanolic extract of diospyros mespiliformis hochst ex a dc ebenaceae and its fractions
Toxicology reports, 2020Co-Authors: A A Ebbo, D Sani, Mohammed Musa Suleiman, A Ahmad, A Z HassanAbstract:Diospyros mespiliformis, commonly called Jackal berry or African ebony, belongs to the plant family, Ebenaceae. The roots, barks and leaves have been used traditionally to treat wide varieties of conditions, however, there is limited information and literature reports concerning the Toxicity and safety of this plant. The present Study was conducted to evaluate the Acute and sub-chronic Toxicity of the crude methanolic extract of Diospyros mespiliformis and its fraction in Wistar rats. Diospyros mespiliformis was extracted by methanol 96 %. The crude methanolic extract was then fractionated into low, average and high polar compounds using hexane, ethyl acetate and butanol respectively. For the Acute Toxicity Study, the revised limit Dose Test of "Up and Down" procedure according to the OECD guideline was used to determine the median lethal dose (LD50) of the crude methanolic leaf and bark extracts using a single fixed dose (5 g/kg) of the extracts administered by oral-gavage sequentially to 5 female Wistar rats. The rats were observed for instant death and Toxicity signs for 24 h and then daily for 14 days. In the sub-chronic Toxicity Study, the bark and leaf ethyl acetate fractions (extract) was administered orally at doses of 250, 500 and 750 mg/kg bw /day respectively for 28 days to healthy Wistar rats. At the end of the experimental period, body weight, certain haematological, serum biochemical and histopathological parameters were evaluated. Results showed that Acute oral administration of crude methanolic extract of Diospyros mespiliformis (5 g/kg bw) produced neither mortality nor visible changes in behavior or any other physiological activities and indicated that the LD50 of crude methanolic leaf and bark extract was greater than 5 g/kg bw in Wistar Rats. In the 28-days repeated dose oral Toxicity Study, no significant toxic effects was detected in any of the parameters evaluated. In conclusion, the crude methanolic extract was found safe in the Acute Toxicity Study and the ethyl acetate fraction of Diospyros mespiliformis in the sub chronic Study in rats could be safe for therapeutic purposes over a period not exceeding 28 days.
Jafri Malin Abdullah - One of the best experts on this subject based on the ideXlab platform.
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Acute Toxicity Study of the standardized methanolic extract of mitragyna speciosa korth in rodent
Journal of Ethnopharmacology, 2010Co-Authors: S N Harizal, J Hasnan, Sharif Mahsufi Mansor, John Tharakan, Jafri Malin AbdullahAbstract:Abstract Ethnopharmacological relevance: Mitragyna speciosa Korth (ketum) is widely used in Malaysia as a medicinal agent for treating diarrhea, worm infestations and also acts as an analgesic and antipyretic. Aim The aim of the Study is to determine the Acute Toxicity of Mitragyna speciosa Korth standardized methanol extract in vivo in 4-weeks-old Sprague–Dawley rats. Methodology Rats were orally administrated single dose of 100, 500 and 1000 mg/kg Mitragyna speciosa Korth standardized methanol extract and the control group received 430 mg/kg of morphine orally. There were 10 rats in each group. All animals were sacrificed after 14 days of treatment. Eight parameters were tested: cage side observation, body weight measurement, food and water consumption, blood pressure, absolute and relative organ weight, hematology, biochemical analysis and histopathology, to look for evidence of Toxicity. Result No mortality was noted after 14 days of treatment. In general, behavior, food and water consumption, hematological studies and organ weights showed no significant changes. The standardized methanol extraction of Mitragyna speciosa Korth increased rat blood pressure (systolic: 147.4 ± 1.01, 131.64 ± 4.94 and 137.8 ± 4.46) after an hour of 100, 500 and 1000 mg/kg doses, respectively. Biochemical studies showed significant elevation of ALT, AST, albumin, triglycerides, cholesterol and albumin ( p > 0.05), at all levels of doses. But, nephroToxicity evidenced by elevated creatinine was seen only at a dose of 1000 mg/kg. Histological examination showed congestion of sinusoids, hemorrhage hepatocytes, fatty change, centrilobular necrosis and increased number of Kuppfer cells in the liver of all Mitragyna speciosa Korth standardized methanol extract treated groups. Conclusion Oral administration of standardized methanolic extraction of Mitragyna speciosa Korth resulted in increasing rat blood pressure after an hour of drug administration. The highest dose of extract also induced Acute severe hepatoToxicity and mild nephroToxicity. However, Mitragyna speciosa Korth shows no effects on body weight, food and water consumption, absolute and relative organ weight and also hematology parameters.
Mikhail I Shtilman - One of the best experts on this subject based on the ideXlab platform.
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amphiphilic poly n vinylpyrrolidone nanoparticles as carriers for non steroidal anti inflammatory drugs in vitro cytoToxicity and in vivo Acute Toxicity Study
Nanomedicine: Nanotechnology Biology and Medicine, 2017Co-Authors: A N Kuskov, Pavel P Kulikov, Mikhail I Shtilman, Anastasia V Goryachaya, Manolis Tzatzarakis, Anca Oana Docea, Kelly Velonia, Aristidis TsatsakisAbstract:Polymeric nanoparticles were prepared from self-assembled amphiphilic N-vinylpyrrolidone polymers in aqueous media and evaluated as novel carriers of indomethacin, a non-steroidal, anti-inflammatory drug. It was determined that these nanoparticles could be created in spherical morphologies with sizes less than 100nm, narrow size distributions and high indomethacin contents(up to 35%) combined with high drug loading efficiencies(up to 95%). In cytoToxicity tests using the human embryonic stem cell derived fibroblasts (EBF-H9) and hepatocellular carcinoma cells (HepG2), the indomethacin-loaded polymeric nanoparticles showed higher cell viability compared to that of free indomethacin at the same concentration. The median LD50 values, determined by the Litchfield-Wilcoxon method, were 55-70mg/kg body weight depending on the polymer molecular design in both mice and rats. Based on the acquired results, these novel amphiphilic poly-N-vinylpyrrolidone nanoparticles can be considered as potential carriers for new, highly efficient, injectable drug delivery systems for hydrophobic drugs such as indomethacin.
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amphiphilic poly n vynilpyrrolidone nanoparticles cytoToxicity and Acute Toxicity Study
Food and Chemical Toxicology, 2016Co-Authors: A N Kuskov, Pavel P Kulikov, Mikhail I Shtilman, Valerii N Rakitskii, Aristidis TsatsakisAbstract:The aim of the present Study was to evaluate the cytoToxicity against MCF-7 cells and Acute intraperitoneal Toxicity of amphiphilic poly-N-vinylpyrrolidone nanoparticles to confirm possibility of their application for creation of novel drug delivery systems. The effect of cellular uptake of polymeric nanoparticles on human cancer cell line MCF-7 cells was investigated by MTT assay. MTT analysis showed that tested amphiphilic polymers were essentially non-toxic. In Acute Toxicity studies, LD50 and other Toxicity indexes were evaluated, under which no deaths or treatment related complications were observed even in high concentration treatment for 14 days of experiment. For histological analysis, organs of the animals were weighed and examined. No animal died during the Study and no significant changes have been observed regarding body weight, feed consumption, organ weight or histological data. Obtained results show that amphiphilic poly-N-vinylpyrrolidone nanoparticles possessed no Toxicity against cells and in animals after intraperitoneal administration. Thus, amphiphilic PVP nanoparticles demonstrate high potential as carriers for novel high-effective drug delivery systems.