association of adenoid surface biofilm formation and chronic otitis media with effusion

Objectives To investigate bacterial biofilm formation on the adenoid surface of pediatric patients undergoing adenoidectomy and to reveal whether this formation is associated with chronic otitis media with effusion (COME). Design Prospective, single-blinded study with scanning electron microscopy. Setting Diskapi Yildirim Beyazit Research and Educational Hospital, Ankara, Turkey. Patients Thirty-four children between 4 and 15 years of age (mean age, 7.76 years) who had undergone adenoidectomy from June 1 through December 31, 2007, were divided into 2 groups. The first group (17 children) had undergone adenoidectomy alone, whereas the second group (17 children) had undergone adenoidectomy combined with ventilation tube insertion for COME. Main Outcome Measures Scanning electron microscopy was performed to observe biofilm formation on the surface of adenoid tissues. The biofilm formation was graded according to extension. The roles of adenoid size, age, sex, and duration of COME symptoms were evaluated. Results Biofilm formation was detected on all samples. Adenoids removed from patients with COME had higher-grade biofilm formation than the other group ( P  = .001). No correlation was found between adenoid size and biofilm formation. Conclusion In pediatric patients, adenoid surface biofilm formation may be associated with COME etiopathogenesis.

Increased occurrence of IgE+ and FcεRI+ cells in Adenoids from atopic children

Background: To examine the influence of atopy on the different cell populations in Adenoids, we investigated the presence of IgE+ cells, cells expressing the high-affinity receptor for IgE (FceRI), and various other cell populations in adenoid tissue, in atopic and nonatopic children with otitis media with effusion (OME) or adenoid hyperplasia (AH).

Methods: Cryostat sections of Adenoids from 14 atopic and 16 nonatopic children suffering from long-lasting OME (n=15) or obstructive AH (n=15) were investigated with immunohistochemical markers for T-cell subsets, mast cells, eosinophils, plasma cells, CD25, CD1a, IgE, and FceRI.

Results: Sensitization to allergens was correlated to an increase of IgE+ cells in the epithelium (P

increased occurrence of ige and fceri cells in Adenoids from atopic children

Background: To examine the influence of atopy on the different cell populations in Adenoids, we investigated the presence of IgE+ cells, cells expressing the high-affinity receptor for IgE (FceRI), and various other cell populations in adenoid tissue, in atopic and nonatopic children with otitis media with effusion (OME) or adenoid hyperplasia (AH).

Methods: Cryostat sections of Adenoids from 14 atopic and 16 nonatopic children suffering from long-lasting OME (n=15) or obstructive AH (n=15) were investigated with immunohistochemical markers for T-cell subsets, mast cells, eosinophils, plasma cells, CD25, CD1a, IgE, and FceRI.

Results: Sensitization to allergens was correlated to an increase of IgE+ cells in the epithelium (P<0.01), the extrafollicular area (P<0.0001), and the follicles (P<0.001) of the Adenoids and an increase of FceRI+ cells in the extrafollicular area (P<0.01). A minority of the IgE+ cells were plasma cells. No significant differences in cells stained for IgE, FceRI, or the other markers were observed between patients with OME and AH. Conclusions: Atopy is associated with increased numbers of IgE+ and FceRI+ cells in Adenoids irrespective of whether the child has OME or AH.

T‐cell subsets in Adenoids and peripheral blood related to age, otitis media with effusion and allergy

Adenoids and peripheral blood samples from 29 children (20 120 months of age) undergoing adenoidectomy for long-standing otitis media with effusion (OME) (n=16) or obstructive adenoid hyperplasia (n=13) were investigated by flow cytometry for their T-lymphocyte profile. Eleven of the enrolled children were allergic to inhalant and/or food allergens. For the whole group, the percentage of helper T cells belonging to the memory phenotype (CD4 + /CD45RO + cells) was significantly higher in Adenoids than in blood (p

IgE-positive plasma cells are present in Adenoids of atopic children

Conclusions. We demonstrated the presence of IgE+ plasma cells in the Adenoids of atopic children. Our data suggest that Adenoids are capable of local production of IgE and support the role of Adenoids as an inductive site for allergic reactions. Objective. We have previously demonstrated increased numbers of IgE+ cells in the Adenoids of atopic children and also found support for an IL-4-induced class switch to IgE production in Adenoids. In search of further evidence of Adenoids being involved in IgE-mediated sensitization, we investigated the distribution of plasma cells and macrophages positive for IgE in Adenoids. Material and methods. Adenoid tissue from atopic and non-atopic children was examined using immunohistochemical markers for IgE, plasma cells (CD138) and macrophages (CD68). The distribution of positive cells was determined in the extrafollicular area and in the follicles of Adenoids. Co-localization of IgE and CD138+ plasma cells and CD68+ macrophages was examined using immunohistochemical…

Increased occurrence of IgE+ and FcεRI+ cells in Adenoids from atopic children

Background: To examine the influence of atopy on the different cell populations in Adenoids, we investigated the presence of IgE+ cells, cells expressing the high-affinity receptor for IgE (FceRI), and various other cell populations in adenoid tissue, in atopic and nonatopic children with otitis media with effusion (OME) or adenoid hyperplasia (AH).

Methods: Cryostat sections of Adenoids from 14 atopic and 16 nonatopic children suffering from long-lasting OME (n=15) or obstructive AH (n=15) were investigated with immunohistochemical markers for T-cell subsets, mast cells, eosinophils, plasma cells, CD25, CD1a, IgE, and FceRI.

Results: Sensitization to allergens was correlated to an increase of IgE+ cells in the epithelium (P