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Adsorption of Protein

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George M Whitesides – One of the best experts on this subject based on the ideXlab platform.

  • self assembled monolayers that resist the Adsorption of Proteins and the adhesion of bacterial and mammalian cells
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robert G. Chapman, Michael N Liang, Gloria Meluleni, Gerald B Pier, And Donald E Ingber, George M Whitesides
    Abstract:

    This paper examines the hypothesis that surfaces resistant to Protein Adsorption should also be resistant to the adhesion of bacteria (Staphylococcus aureus, Staphylococcus epidepidermidis) and the attachment and spreading of mammalian cells (bovine capillary endothelial (BCE) cells). The surfaces tested were those of self-assembled monolayers (SAMs) terminated with derivatives of tri(sarcosine) (Sarc), N-acetylpiperazine, permethylated sorbitol, hexamethylphosphoramide, phosphoryl choline, and an intramolecular zwitterion (−CH2N+(CH3)2CH2CH2CH2SO3-) (ZW); all are known to resist the Adsorption of Proteins. There seems to be little or no correlation between the Adsorption of Protein (fibrinogen and lysozyme) and the adhesion of cells. Surfaces terminated with derivatives of Sarc and N-acetylpiperazine resisted the adhesion of S. aureus and S. epidermidis as well as did surfaces terminated with tri(ethylene glycol). A surface that presented Sarc groups was the only one that resisted the adhesion of BCE cells a…

  • a survey of structure property relationships of surfaces that resist the Adsorption of Protein
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robe G Chapma, Erik R Holmli, And Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific Adsorption of Proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N-acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the Adsorption of Proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the Adsorption of Proteins. In the group of surfaces examined, those that resisted the Adsorption of Proteins had the following properties:  they were …

  • Zwitterionic SAMs that Resist Nonspecific Adsorption of Protein from Aqueous Buffer
    Langmuir, 2001
    Co-Authors: R. Erik Holmlin, Xiaoxi Chen, Robert G. Chapman, Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) of alkanethiols on gold to evaluate the ability of surfaces terminating in different combinations of charged groups to resist the nonspecific Adsorption of Proteins from aqueous buffer. Mixed SAMs formed from a 1:1 combination of a thiol terminated in a trimethylammonium group and a thiol terminated in a sulfonate group adsorbed less than 1% of a monolayer of two Proteins with different characteristics:  fibrinogen and lysozyme. Single-component SAMs formed from thiols terminating in groups combining a positively charged moiety and a negatively charged moiety were also capable of resisting the Adsorption of Proteins. Single-component SAMs presenting single charges adsorbed nearly a full monolayer of Protein. The amount of Protein that adsorbed to mixed zwitterionic SAMs did not depend on the ionic strength or the pH of the buffer in which the Protein was dissolved. The amount of Protein that adsorbe…

Emanuele Ostuni – One of the best experts on this subject based on the ideXlab platform.

  • self assembled monolayers that resist the Adsorption of Proteins and the adhesion of bacterial and mammalian cells
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robert G. Chapman, Michael N Liang, Gloria Meluleni, Gerald B Pier, And Donald E Ingber, George M Whitesides
    Abstract:

    This paper examines the hypothesis that surfaces resistant to Protein Adsorption should also be resistant to the adhesion of bacteria (Staphylococcus aureus, Staphylococcus epidermidis) and the attachment and spreading of mammalian cells (bovine capillary endothelial (BCE) cells). The surfaces tested were those of self-assembled monolayers (SAMs) terminated with derivatives of tri(sarcosine) (Sarc), N-acetylpiperazine, permethylated sorbitol, hexamethylphosphoramide, phosphoryl choline, and an intramolecular zwitterion (−CH2N+(CH3)2CH2CH2CH2SO3-) (ZW); all are known to resist the Adsorption of Proteins. There seems to be little or no correlation between the Adsorption of Protein (fibrinogen and lysozyme) and the adhesion of cells. Surfaces terminated with derivatives of Sarc and N-acetylpiperazine resisted the adhesion of S. aureus and S. epidermidis as well as did surfaces terminated with tri(ethylene glycol). A surface that presented Sarc groups was the only one that resisted the adhesion of BCE cells a…

  • a survey of structure property relationships of surfaces that resist the Adsorption of Protein
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robe G Chapma, Erik R Holmli, And Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific Adsorption of Proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N-acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the Adsorption of Proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the Adsorption of Proteins. In the group of surfaces examined, those that resisted the Adsorption of Proteins had the following properties:  they were …

  • A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robert G. Chapman, R. Erik Holmlin, And Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific Adsorption of Proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N-acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the Adsorption of Proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the Adsorption of Proteins. In the group of surfaces examined, those that resisted the Adsorption of Proteins had the following properties:  they were …

Robert G. Chapman – One of the best experts on this subject based on the ideXlab platform.

  • self assembled monolayers that resist the Adsorption of Proteins and the adhesion of bacterial and mammalian cells
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robert G. Chapman, Michael N Liang, Gloria Meluleni, Gerald B Pier, And Donald E Ingber, George M Whitesides
    Abstract:

    This paper examines the hypothesis that surfaces resistant to Protein Adsorption should also be resistant to the adhesion of bacteria (Staphylococcus aureus, Staphylococcus epidermidis) and the attachment and spreading of mammalian cells (bovine capillary endothelial (BCE) cells). The surfaces tested were those of self-assembled monolayers (SAMs) terminated with derivatives of tri(sarcosine) (Sarc), N-acetylpiperazine, permethylated sorbitol, hexamethylphosphoramide, phosphoryl choline, and an intramolecular zwitterion (−CH2N+(CH3)2CH2CH2CH2SO3-) (ZW); all are known to resist the Adsorption of Proteins. There seems to be little or no correlation between the Adsorption of Protein (fibrinogen and lysozyme) and the adhesion of cells. Surfaces terminated with derivatives of Sarc and N-acetylpiperazine resisted the adhesion of S. aureus and S. epidermidis as well as did surfaces terminated with tri(ethylene glycol). A surface that presented Sarc groups was the only one that resisted the adhesion of BCE cells a…

  • Zwitterionic SAMs that Resist Nonspecific Adsorption of Protein from Aqueous Buffer
    Langmuir, 2001
    Co-Authors: R. Erik Holmlin, Xiaoxi Chen, Robert G. Chapman, Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) of alkanethiols on gold to evaluate the ability of surfaces terminating in different combinations of charged groups to resist the nonspecific Adsorption of Proteins from aqueous buffer. Mixed SAMs formed from a 1:1 combination of a thiol terminated in a trimethylammonium group and a thiol terminated in a sulfonate group adsorbed less than 1% of a monolayer of two Proteins with different characteristics:  fibrinogen and lysozyme. Single-component SAMs formed from thiols terminating in groups combining a positively charged moiety and a negatively charged moiety were also capable of resisting the Adsorption of Proteins. Single-component SAMs presenting single charges adsorbed nearly a full monolayer of Protein. The amount of Protein that adsorbed to mixed zwitterionic SAMs did not depend on the ionic strength or the pH of the buffer in which the Protein was dissolved. The amount of Protein that adsorbe…

  • A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robert G. Chapman, R. Erik Holmlin, And Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific Adsorption of Proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N-acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the Adsorption of Proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the Adsorption of Proteins. In the group of surfaces examined, those that resisted the Adsorption of Proteins had the following properties:  they were …

Anne L. Plant – One of the best experts on this subject based on the ideXlab platform.

  • Biotechnological applications of surface plasmon resonance
    Trends in Biotechnology, 1997
    Co-Authors: Vitalii Silin, Anne L. Plant
    Abstract:

    The surface plasmon resonance technique allows direct, real-time kinetic measurements of the interaction of unlabelled biological molecules at surfaces. After a brief discussion of the principles of surface plasmon resonance, we review its application to the nonspecific Adsorption of Protein, the formation of phospholipid bilayers, membrane-Protein interactions and DNA hybrhybridization. The morphological studies of the biological surfaces using surface plasmon resonance microscopy and the potential of the surface plasmon resonance to measure the dynamics of surface layer heterogeneity are discussed.

R. Erik Holmlin – One of the best experts on this subject based on the ideXlab platform.

  • Zwitterionic SAMs that Resist Nonspecific Adsorption of Protein from Aqueous Buffer
    Langmuir, 2001
    Co-Authors: R. Erik Holmlin, Xiaoxi Chen, Robert G. Chapman, Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) of alkanethiols on gold to evaluate the ability of surfaces terminating in different combinations of charged groups to resist the nonspecific Adsorption of Proteins from aqueous buffer. Mixed SAMs formed from a 1:1 combination of a thiol terminated in a trimethylammonium group and a thiol terminated in a sulfonate group adsorbed less than 1% of a monolayer of two Proteins with different characteristics:  fibrinogen and lysozyme. Single-component SAMs formed from thiols terminating in groups combining a positively charged moiety and a negatively charged moiety were also capable of resisting the Adsorption of Proteins. Single-component SAMs presenting single charges adsorbed nearly a full monolayer of Protein. The amount of Protein that adsorbed to mixed zwitterionic SAMs did not depend on the ionic strength or the pH of the buffer in which the Protein was dissolved. The amount of Protein that adsorbe…

  • A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
    Langmuir, 2001
    Co-Authors: Emanuele Ostuni, Robert G. Chapman, R. Erik Holmlin, And Shuichi Takayama, George M Whitesides
    Abstract:

    This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific Adsorption of Proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N-acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the Adsorption of Proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the Adsorption of Proteins. In the group of surfaces examined, those that resisted the Adsorption of Proteins had the following properties:  they were …