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Claude Teisson - One of the best experts on this subject based on the ideXlab platform.

  • comparison of methods of Liquid medium culture for banana micropropagation effects of temporary immersion of explants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Daniel Alvard, Francoisxavier Cote, Claude Teisson
    Abstract:

    Five different Liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); Liquid medium with immersion of the plants (treatment 2); Liquid medium with cellulose culture support (treatment 3); Liquid medium with partial immersion of the plants (treatment 4); Liquid medium Aerated by bubbling (treatment 5); Liquid medium with temporary immersion of the explants for 20 min every 2h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: -shoots in simple Liquid medium and those on cellulose substrate proliferated little or not at all, -shoots on gelled medium, those subjected to partial immersion and those in Aerated medium displayed multiplication rates of 2.2 to 3.1, and - the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatments 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in Aerated Liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in Aerated treatments (treatments 4 and 5). Shoots in Liquid medium continuously Aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants.

  • comparison of methods of Liquid medium culture for banana micropropagation effects of temporary immersion of explants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Daniel Alvard, Francoisxavier Cote, Claude Teisson
    Abstract:

    Five different Liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); Liquid medium with immersion of the plants (treatment 2); Liquid medium with cellulose culture support (treatment 3); Liquid medium with partial immersion of the plants (treatment 4); Liquid medium Aerated by bubbling (treatment 5); Liquid medium with temporary immersion of the explants for 20 min every 2 h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: - shoots in simple Liquid medium and those on cellulose substrate proliferated little or not at all, - shoots on gelled medium, those subjected to partial immersion and those in Aerated medium displayed multiplication rates of 2.2 to 3.1, and - the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatments 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in Aerated Liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in Aerated treatments (treatments 4 and 5). Shoots in Liquid medium continuously Aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants. (Resume d'auteur)

Daniel Alvard - One of the best experts on this subject based on the ideXlab platform.

  • comparison of methods of Liquid medium culture for banana micropropagation effects of temporary immersion of explants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Daniel Alvard, Francoisxavier Cote, Claude Teisson
    Abstract:

    Five different Liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); Liquid medium with immersion of the plants (treatment 2); Liquid medium with cellulose culture support (treatment 3); Liquid medium with partial immersion of the plants (treatment 4); Liquid medium Aerated by bubbling (treatment 5); Liquid medium with temporary immersion of the explants for 20 min every 2h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: -shoots in simple Liquid medium and those on cellulose substrate proliferated little or not at all, -shoots on gelled medium, those subjected to partial immersion and those in Aerated medium displayed multiplication rates of 2.2 to 3.1, and - the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatments 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in Aerated Liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in Aerated treatments (treatments 4 and 5). Shoots in Liquid medium continuously Aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants.

  • comparison of methods of Liquid medium culture for banana micropropagation effects of temporary immersion of explants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Daniel Alvard, Francoisxavier Cote, Claude Teisson
    Abstract:

    Five different Liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); Liquid medium with immersion of the plants (treatment 2); Liquid medium with cellulose culture support (treatment 3); Liquid medium with partial immersion of the plants (treatment 4); Liquid medium Aerated by bubbling (treatment 5); Liquid medium with temporary immersion of the explants for 20 min every 2 h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: - shoots in simple Liquid medium and those on cellulose substrate proliferated little or not at all, - shoots on gelled medium, those subjected to partial immersion and those in Aerated medium displayed multiplication rates of 2.2 to 3.1, and - the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatments 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in Aerated Liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in Aerated treatments (treatments 4 and 5). Shoots in Liquid medium continuously Aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants. (Resume d'auteur)

Francoisxavier Cote - One of the best experts on this subject based on the ideXlab platform.

  • comparison of methods of Liquid medium culture for banana micropropagation effects of temporary immersion of explants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Daniel Alvard, Francoisxavier Cote, Claude Teisson
    Abstract:

    Five different Liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); Liquid medium with immersion of the plants (treatment 2); Liquid medium with cellulose culture support (treatment 3); Liquid medium with partial immersion of the plants (treatment 4); Liquid medium Aerated by bubbling (treatment 5); Liquid medium with temporary immersion of the explants for 20 min every 2h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: -shoots in simple Liquid medium and those on cellulose substrate proliferated little or not at all, -shoots on gelled medium, those subjected to partial immersion and those in Aerated medium displayed multiplication rates of 2.2 to 3.1, and - the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatments 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in Aerated Liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in Aerated treatments (treatments 4 and 5). Shoots in Liquid medium continuously Aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants.

  • comparison of methods of Liquid medium culture for banana micropropagation effects of temporary immersion of explants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Daniel Alvard, Francoisxavier Cote, Claude Teisson
    Abstract:

    Five different Liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); Liquid medium with immersion of the plants (treatment 2); Liquid medium with cellulose culture support (treatment 3); Liquid medium with partial immersion of the plants (treatment 4); Liquid medium Aerated by bubbling (treatment 5); Liquid medium with temporary immersion of the explants for 20 min every 2 h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: - shoots in simple Liquid medium and those on cellulose substrate proliferated little or not at all, - shoots on gelled medium, those subjected to partial immersion and those in Aerated medium displayed multiplication rates of 2.2 to 3.1, and - the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatments 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in Aerated Liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in Aerated treatments (treatments 4 and 5). Shoots in Liquid medium continuously Aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants. (Resume d'auteur)

Jun Zhu - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of a low cost surface aeration system in an anaerobic poultry lagoon
    2009 Reno Nevada June 21 - June 24 2009, 2009
    Co-Authors: Saqib Mukhtar, M. S. Borhan, Shafiqur Rahman, Jun Zhu
    Abstract:

    Odor from animal feeding operations (AFOs) is a leading public nuisance that livestock and poultry producers face frequently. The objective of this study was to evaluate a field-scale surface aeration system to minimize odor from an anaerobic poultry lagoon in Texas. The aeration module consisted of three aerators, each aerator contained two venturi air injectors arranged in parallel and connected to a perforated PVC pipe network that Aerated a site of 23m × 23m. The pipe network in the aeration system was placed 0.30 m below the Liquid surface for aerating the lagoon. To compare the effect of aeration on lagoon odors and other parameters, a non-Aerated (control) site was established about 30 m away from the Aerated site to minimize treatment interference. Automatic samplers were used to collect effluent samples from each site at a depth of 0.30m below the Liquid surface once a week for 14 weeks, and analyzed for changes in biochemical oxygen demand (BOD5), volatile fatty acids (VFAs), solids, and nutrient concentrations. Real-time dissolved oxygen and water temperature were also monitored by deploying sensors at the same depth. Six air samples (three each from Aerated and non-Aerated sites) were collected once a week in 10 L Tedlar bags and were analyzed for odors (dilution to threshold, DT), ammonia (NH3) and hydrogen sulfide (H2S) concentrations. Average DO and NH3 concentrations from Aerated Liquid were significantly (p 0.5). There were no significant differences in solids and nutrient concentrations between Aerated and non-Aerated site. These results demonstrated that surface aeration is effectively reducing odor and H2S concentrations and increasing NH3 concentrations in the Aerated site as compared to the non-Aerated. Struvite build-up in the lagoon Liquid circulation pump, venturi injectors and the PVC pipe network, and clogging of strainers and PVC pipe aeration holes with lagoon surface scum and egg-shells required frequent cleaning and maintenance of the aeration system.

  • characteristics of solids bod5 and vfas in Liquid swine manure treated by short term low intensity aeration for long term storage
    Bioresource Technology, 2006
    Co-Authors: Zhijian Zhang, Jun Zhu
    Abstract:

    A laboratory-scale experiment presents data that reveal the temporal characteristics of solids, biochemical oxygen demand (BOD5) and volatile fatty acids (VFAs) in the Aerated Liquid swine manure for minimizing odor generation potential during 190day storage. The performance of 15-day aeration of Liquid manure with initial total solids (TS) content from 0.5% to 4.0% was examined at low-intensity aeration rates, i.e., +35 mV oxidation–reduction potential (ORP), 1.0 mg O2/l and 3.0 mg O2/l dissolved oxygen (DO). Odor generation potential was evaluated using VFAs. The aeration process contributed remarkably to the decomposition of TS, total volatile solids (TVS), BOD5 and VFAs. Moreover, the stabilization of manure due to aeration could last up to 190 days. The TS reduction on day 190 ranging from 6.3% to 32.7%, 20.2% to 39.1%, 19.0% to 41.0% were realized under the intensities of +35 mV ORP, 1.0 and 3.0 mg O2/l, respectively. At the same time, the reduction of BOD5 and VFAs reached around 7.8% to 69.5%, 17.2% to 79.9% and 21.9% to 91.1%; 0.4% to 91.0%, 60.4% to 95.0% and 70.4% to 94.1%. The Liquid manure with low solids (e.g., TS of 0.5% and 1.0%) offered an advantageous condition for aeration treatment, particularly for biodegradation of BOD5 and VFAs. The odor generation potential could also be evaluated by the levels of solids and BOD5 in the manure. Increasing aeration intensity would significantly diminish the odor generation potential for given levels of solids and/or BOD5. Fifteen-day aeration with intensity of 1.0 mg O2/l may be recommended at farm level for both odor control and energy savings. 2005 Elsevier Ltd. All rights reserved.

Eliane Romanato Santarem - One of the best experts on this subject based on the ideXlab platform.

  • secondary metabolism in micropropagated hypericum perforatum l grown in non Aerated Liquid medium
    Plant Cell Tissue and Organ Culture, 2012
    Co-Authors: Luiz Eduardo Baggio Savio, Leandro Vieira Astarita, Eliane Romanato Santarem
    Abstract:

    Hypericum perforatum L. is a medicinal plant that has been extensively studied because of its bioactive properties. The objective of this study was to establish a system that could lower the cost of in vitro propagation by using Liquid medium, as well as to evaluate the secondary metabolism in the systems tested. Nodal segments of H. perforatum were obtained from in vitro shoots and grown in three Liquid culture systems: total immersion (TI), partial immersion (PI), and paper bridge support (PB). Semi-solid medium (3 g L−1 Phytagel™) was used as control (SS). The organogenic responses were evaluated, and phenolic compounds, hypericin, and the activity of polyphenol oxidases (PPO) and peroxidases (POX) were quantified. After 80 days of culture, induction and proliferation of adventitious shoots were similar in the PI and SS systems (65.3 and 71.3 shoots, respectively), whereas PB resulted in the fewest shoots per explant (29.5 shoots). Longer shoots were obtained under the PI conditions. Hyperhydricity was observed in the shoots from the TI system. Browning was visible in shoots from the TI and PB systems. The highest concentrations of phenolic compounds and hypericin were observed in shoots derived from PI and PB, at 80 days of culture. POX activity was higher in shoots cultured in PI at 40 days, whereas PPO was significantly more active at 80 days of culture. Likely, POX was more related to shoot growth, whereas PPO played a later role in response to the culture environment and medium stress.