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Jie Lv - One of the best experts on this subject based on the ideXlab platform.
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moheibacter sediminis gen nov sp nov a member of the family flavobacteriaceae isolated from sediment and emended descriptions of empedobacter brevis wautersiella falsenii and weeksella virosa
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Rengang Zhang, Xingmin Zhao, Jian Deng, Jie LvAbstract:A Gram-reaction-negative, yellow-pigmented, strictly Aerobic Bacterium, designated M0116T, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116T was a member of the family Flavobacteriaceae and was most closely related to members of the genera Empedobacter , Wautersiella and Weeksella with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Moheibacter sediminis gen. nov., sp. nov. is proposed. The type strain is M0116T ( = CGMCC 1.12708T = JCM 19634T). Emended descriptions of Empedobacter brevis , Wautersiella falsenii and Weeksella virosa are also proposed.
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moheibacter sediminis gen nov sp nov a member of the family flavobacteriaceae isolated from sediment and emended descriptions of empedobacter brevis wautersiella falsenii and weeksella virosa
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Rengang Zhang, Xingmin Zhao, Jian Deng, Jie LvAbstract:A Gram-reaction-negative, yellow-pigmented, strictly Aerobic Bacterium, designated M0116T, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116T was a member of the family Flavobacteriaceae and was most closely related to members of the genera Empedobacter , Wautersiella and Weeksella with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Moheibacter sediminis gen. nov., sp. nov. is proposed. The type strain is M0116T ( = CGMCC 1.12708T = JCM 19634T). Emended descriptions of Empedobacter brevis , Wautersiella falsenii and Weeksella virosa are also proposed.
Juergen Wiegel - One of the best experts on this subject based on the ideXlab platform.
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anoxybacillus kamchatkensis sp nov a novel thermophilic facultative Aerobic Bacterium with a broad ph optimum from the geyser valley kamchatka
Extremophiles, 2005Co-Authors: V V Kevbrin, Karsten Zengler, Anatolii M Lysenko, Juergen WiegelAbstract:A facultative Aerobic, moderately thermophilic, spore forming Bacterium, strain JW/VK-KG4 was isolated from an enrichment culture obtained from the Geyser valley, a geothermally heated environment located in the Kamchatka peninsula (Far East region of Russia). The cells were rod shaped, motile, peritrichous flagellated stained Gram positive and had a Gram positive type cell wall. Aerobically, the strain utilized a range of carbohydrates including glucose, fructose, trehalose, proteinuous substrates, and pectin as well. AnAerobically, only carbohydrates are utilized. When growing on carbohydrates, the strain required yeast extract and vitamin B12. AnAerobically, glucose was fermented to lactate as main product and acetate, formate, ethanol as minor products. Aerobically, even in well-aerated cultures (agitated at 500 rpm), glucose oxidation was incomplete and lactate and acetate were found in culture supernatants as by-products. Optimal growth of the isolate was observed at pH25 C 6.8–8.5 and 60°C. The doubling times on glucose at optimal growth conditions were 34 min (Aerobically) and 40 min (anAerobically). The G+C content was 42.3 mol% as determined by Tm assay. Sequence analysis of the 16S rRNA gene indicated an affiliation of strain JW/VK-KG4 with Anoxybacillus species. Based on its morphology, physiology, phylogenetic relationship and its low DNA-DNA homology with validly published species of Anoxybacillus, it is proposed that strain JW/VK-KG4 represents a new species in the genus Anoxybacillus as A. kamchatkensis sp. nov. The type strain for the novel species is JW/VK-KG4T (=DSM 14988, =ATCC BAA-549). The GenBank accession number for the 16S rDNA sequence is AF510985.
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anoxybacillus kamchatkensis sp nov a novel thermophilic facultative Aerobic Bacterium with a broad ph optimum from the geyser valley kamchatka
Extremophiles, 2005Co-Authors: V V Kevbrin, Karsten Zengler, Anatolii M Lysenko, Juergen WiegelAbstract:A facultative Aerobic, moderately thermophilic, spore forming Bacterium, strain JW/VK-KG4 was isolated from an enrichment culture obtained from the Geyser valley, a geothermally heated environment located in the Kamchatka peninsula (Far East region of Russia). The cells were rod shaped, motile, peritrichous flagellated stained Gram positive and had a Gram positive type cell wall. Aerobically, the strain utilized a range of carbohydrates including glucose, fructose, trehalose, proteinuous substrates, and pectin as well. AnAerobically, only carbohydrates are utilized. When growing on carbohydrates, the strain required yeast extract and vitamin B12. AnAerobically, glucose was fermented to lactate as main product and acetate, formate, ethanol as minor products. Aerobically, even in well-aerated cultures (agitated at 500 rpm), glucose oxidation was incomplete and lactate and acetate were found in culture supernatants as by-products. Optimal growth of the isolate was observed at pH25 C 6.8–8.5 and 60°C. The doubling times on glucose at optimal growth conditions were 34 min (Aerobically) and 40 min (anAerobically). The G+C content was 42.3 mol% as determined by Tm assay. Sequence analysis of the 16S rRNA gene indicated an affiliation of strain JW/VK-KG4 with Anoxybacillus species. Based on its morphology, physiology, phylogenetic relationship and its low DNA-DNA homology with validly published species of Anoxybacillus, it is proposed that strain JW/VK-KG4 represents a new species in the genus Anoxybacillus as A. kamchatkensis sp. nov. The type strain for the novel species is JW/VK-KG4T (=DSM 14988, =ATCC BAA-549). The GenBank accession number for the 16S rDNA sequence is AF510985.
Rengang Zhang - One of the best experts on this subject based on the ideXlab platform.
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moheibacter sediminis gen nov sp nov a member of the family flavobacteriaceae isolated from sediment and emended descriptions of empedobacter brevis wautersiella falsenii and weeksella virosa
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Rengang Zhang, Xingmin Zhao, Jian Deng, Jie LvAbstract:A Gram-reaction-negative, yellow-pigmented, strictly Aerobic Bacterium, designated M0116T, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116T was a member of the family Flavobacteriaceae and was most closely related to members of the genera Empedobacter , Wautersiella and Weeksella with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Moheibacter sediminis gen. nov., sp. nov. is proposed. The type strain is M0116T ( = CGMCC 1.12708T = JCM 19634T). Emended descriptions of Empedobacter brevis , Wautersiella falsenii and Weeksella virosa are also proposed.
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moheibacter sediminis gen nov sp nov a member of the family flavobacteriaceae isolated from sediment and emended descriptions of empedobacter brevis wautersiella falsenii and weeksella virosa
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Rengang Zhang, Xingmin Zhao, Jian Deng, Jie LvAbstract:A Gram-reaction-negative, yellow-pigmented, strictly Aerobic Bacterium, designated M0116T, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116T was a member of the family Flavobacteriaceae and was most closely related to members of the genera Empedobacter , Wautersiella and Weeksella with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Moheibacter sediminis gen. nov., sp. nov. is proposed. The type strain is M0116T ( = CGMCC 1.12708T = JCM 19634T). Emended descriptions of Empedobacter brevis , Wautersiella falsenii and Weeksella virosa are also proposed.
Shixue Zheng - One of the best experts on this subject based on the ideXlab platform.
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novel mechanisms of selenate and selenite reduction in the obligate Aerobic Bacterium comamonas testosteroni s44
Journal of Hazardous Materials, 2018Co-Authors: Yuanqing Tan, Dan Wang, Gejiao Wang, Christopher Rensing, Yuantao Wang, Yu Wang, Yeting Huang, Shixue ZhengAbstract:Abstract Selenium oxyanion reduction is an effective detoxification or/and assimilation processes in organisms, but little is known the mechanisms in Aerobic bacteria. Aerobic Comamonas testosteroni S44 reduces Se(VI)/Se(IV) to less-toxic elemental selenium nanoparticles (SeNPs). For Se(VI) reduction, sulfate and Se(VI) reduction displayed a competitive relationship. When essential sulfate reducing genes were respectively disrupted, Se(VI) was not reduced to red-colored SeNPs. Consequently, Se(VI) reduction was catalyzed by enzymes of the sulfate reducing pathway. For Se(IV) reduction, one of the potential periplasm molybdenum oxidoreductase named SerT was screened and further used to analyze Se(IV) reduction. Compared to the wild type and the complemented mutant strain, the ability of Se(IV) reduction was reduced 75% in the deletion mutant ΔserT. Moreover, the Se(IV) reduction rate was significantly enhanced when the gene serT was overexpressed in Escherichia coli W3110. In addition, Se(IV) was reduced to SeNPs by the purified SerT with the presence of NADPH as the electron donor in vitro, showing a Vmax of 61 nmol/min·mg and a Km of 180 μmol/L. A model of Se(VI)/Se(IV) reduction was generated in Aerobic C. testosteroni S44. This work provides new insights into the molecular mechanisms of Se(VI)/Se(IV) reduction activities in Aerobic bacteria.
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selenite reduction by the obligate Aerobic Bacterium comamonas testosteroni s44 isolated from a metal contaminated soil
BMC Microbiology, 2014Co-Authors: Shixue Zheng, Liang Wang, Rong Yao, Dan Wang, Yujia Deng, Rui Wang, Gejiao Wang, Christopher RensingAbstract:Selenium (Se) is an essential trace element in most organisms but has to be carefully handled since there is a thin line between beneficial and toxic concentrations. Many bacteria have the ability to reduce selenite (Se(IV)) and (or) selenate (Se(VI)) to red elemental selenium that is less toxic. A strictly Aerobic Bacterium, Comamonas testosteroni S44, previously isolated from metal(loid)-contaminated soil in southern China, reduced Se(IV) to red selenium nanoparticles (SeNPs) with sizes ranging from 100 to 200 nm. Both energy dispersive X-ray Spectroscopy (EDX or EDS) and EDS Elemental Mapping showed no element Se and SeNPs were produced inside cells whereas Se(IV) was reduced to red-colored selenium in the cytoplasmic fraction in presence of NADPH. Tungstate inhibited Se(VI) but not Se(IV) reduction, indicating the Se(IV)-reducing determinant does not contain molybdenum as co-factor. Strain S44 was resistant to multiple heavy and transition metal(loid)s such as Se(IV), As(III), Cu(II), and Cd(II) with minimal inhibitory concentrations (MIC) of 100 mM, 20 mM, 4 mM, and 0.5 mM, respectively. Disruption of iscR encoding a transcriptional regulator negatively impacted cellular growth and subsequent resistance to multiple heavy metal(loid)s. C. testosteroni S44 could be very useful for bioremediation in heavy metal(loid) polluted soils due to the ability to both reduce toxic Se(VI) and Se(IV) to non-toxic Se (0) under Aerobic conditions and to tolerate multiple heavy and transition metals. IscR appears to be an activator to regulate genes involved in resistance to heavy or transition metal(loid)s but not for genes responsible for Se(IV) reduction.
Akira Hiraishi - One of the best experts on this subject based on the ideXlab platform.
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chryseoBacterium shigense sp nov a yellow pigmented Aerobic Bacterium isolated from a lactic acid beverage
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Kengo Shimomura, Shigeo Kaji, Akira HiraishiAbstract:A yellow-pigmented Bacterium designated strain GUM-Kaji T was isolated from a lactic acid beverage. The strain had Gram-negative, non-motile, rod-shaped cells. It was strictly Aerobic and chemo-organotrophic and grew at 5–30 6C and at pH 5–8. The major components of the non-polar and 3-hydroxy fatty acids were C15 : 0 iso and 3-OH-C17 : 0 iso, respectively. Menaquinone MK-6 was detected as the sole quinone. 16S rRNA gene sequence comparisons revealed that strain GUM-Kaji T is affiliated to the genus ChryseoBacterium, with ChryseoBacterium joostei as its phylogenetic neighbour, but there were low levels of similarity (<96 %) to any established species of the genus. The G+C content of the genomic DNA was 36?6 mol%. The novel Bacterium differed from any known species of ChryseoBacterium in terms of a number of phenotypic properties. Thus, the name ChryseoBacterium shigense sp. nov. is proposed for this novel Bacterium. The type strain is strain GUM-Kaji T (=BAMY 1001 T =NCIMB 14047 T =DSM 17126 T ).
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chryseoBacterium shigense sp nov a yellow pigmented Aerobic Bacterium isolated from a lactic acid beverage
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Kengo Shimomura, Shigeo Kaji, Akira HiraishiAbstract:A yellow-pigmented Bacterium designated strain GUM-Kaji(T) was isolated from a lactic acid beverage. The strain had Gram-negative, non-motile, rod-shaped cells. It was strictly Aerobic and chemo-organotrophic and grew at 5-30 degrees C and at pH 5-8. The major components of the non-polar and 3-hydroxy fatty acids were C15:0 iso and 3-OH-C17:0 iso, respectively. Menaquinone MK-6 was detected as the sole quinone. 16S rRNA gene sequence comparisons revealed that strain GUM-Kaji(T) is affiliated to the genus ChryseoBacterium, with ChryseoBacterium joostei as its phylogenetic neighbour, but there were low levels of similarity (<96%) to any established species of the genus. The G+C content of the genomic DNA was 36.6 mol%. The novel Bacterium differed from any known species of ChryseoBacterium in terms of a number of phenotypic properties. Thus, the name ChryseoBacterium shigense sp. nov. is proposed for this novel Bacterium. The type strain is strain GUM-Kaji(T) (=BAMY 1001(T)=NCIMB 14047(T)=DSM 17126(T)).
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discovery of natural photosynthesis using zn containing bacteriochlorophyll in an Aerobic Bacterium acidiphilium rubrum
Plant and Cell Physiology, 1996Co-Authors: Norio Wakao, Akira Hiraishi, Naoto Yokoi, Naohito Isoyama, Keizo Shimada, Masami Kobayashi, Hideo Kise, Masayo Iwaki, Shigeru Itoh, Shinichi TakaichiAbstract:We discovered natural photosynthesis using Zn-containing bacteriochlorophyll a in an acidophilic Bacterium Addiphilium rubrum. Chemical analysis of the cell extracts gave a 13 : 2 :1 molar ratio of Zn-bacterioch lorophyll a : Mg-bacteriochlorophyll a : bacteriopheophytin a. Most of the pigments are associated with fully active reaction center and light-harvesting complexes analogous to those in purple photosynthetic bacteria. The finding indicates an unexpectedly wide variability of photosynthesi s.