The Experts below are selected from a list of 60 Experts worldwide ranked by ideXlab platform

Toshio Ohhashi - One of the best experts on this subject based on the ideXlab platform.

  • Real-time imaging of the Lymphatic channels and sentinel Lymph nodes of the stomach using contrast-enhanced ultrasonography with Sonazoid in a porcine model.
    Cancer science, 2011
    Co-Authors: Yoshiko Kawai, Kumiko Ajima, Takashi Nagai, Maki Kaidoh, Toshio Ohhashi
    Abstract:

    The contrast-enhanced ultrasound (CEUS)-guided method in combination with Sonazoid has not been clinically or experimentally evaluated with regard to its use for identifying sentinel Lymph node (SLN) in the stomach. Therefore, we attempted to evaluate the usefulness of the CEUS-guided method with Sonazoid for imaging of the Lymphatic channels and SLN of the stomach in a porcine model by comparing it with the conventional Evans blue dye-guided method. Twenty-eight 2 to 3-month-old swine weighing 17-30 kg were used in this experiment. Anesthesia was maintained with 2.0-3.0% isoflurane/O(2) inhalation. Sonazoid was injected into the intra- and sub-mucosal layers of the stomach. The intragastric or transcutaneous CEUS-guided method was used to identify the Lymphatic channels and SLN of the stomach. Contrast imaging using the CEUS-guided method with Sonazoid enabled us to produce clear images of the Afferent Lymph Vessel and SLN of the stomach until 2 h after the injection of Sonazoid. In addition, intranodal flow of the microbubble agent could be clearly identified using tissue linear harmonic images of the SLN. The SLN detection rate was not significantly different between the CEUS- and dye-guided methods. However, the Evans blue dye flowed out quickly (≈ 15 min after the injection) through the true SLN into the next LN of stomach. In conclusion, the use of the CEUS-guided method with Sonazoid might be the most useful clinical procedure for producing real-time images of the SLN of the stomach, and the linear harmonic images are also useful for evaluating intranodal structure within the SLN.

T J Heath - One of the best experts on this subject based on the ideXlab platform.

  • effects of the adjuvant deaf dextran and staphylococcus aureus on the Lymph pathways and filtering capacity of popliteal Lymph nodes in sheep
    Research in Veterinary Science, 1991
    Co-Authors: H J Spalding, T J Heath
    Abstract:

    The effects of subcutaneous injections of the adjuvant DEAF-dextran and, or killed Staphylococcus aureus on the structure of the Lymph pathways of popliteal Lymph nodes in sheep were examined using light and electron microscopy and Microfil casts. Dextran with or without killed S aureus caused significant changes in the Lymph pathways both within the node and outside it. However, killed S aureus alone did not. The changes included anastomoses among Afferent Lymph Vessels and between Afferent and efferent Lymph Vessels; proliferation of Vessels around the node; joining of parts of the capsule and trabeculae to adjacent parenchyma with loss of parts of the subcapsular and trabecular sinuses; enlargement of medullary sinuses; and reduction of the number of reticular processes in sinuses throughout the node. These changes were accompanied by a reduced ability of the node to filter chicken red blood cells labelled with chromium-51 which were injected into an Afferent Lymph Vessel.

  • effects of the adjuvant deaf dextran and staphylococcus aureus on the Lymph pathways and filtering capacity of popliteal Lymph nodes in sheep
    Research in Veterinary Science, 1991
    Co-Authors: H J Spalding, T J Heath
    Abstract:

    The effects of subcutaneous injections of the adjuvant DEAF-dextran and, or killed Staphylococcus aureus on the structure of the Lymph pathways of popliteal Lymph nodes in sheep were examined using light and electron microscopy and Microfil casts. Dextran with or without killed S aureus caused significant changes in the Lymph pathways both within the node and outside it. However, killed S aureus alone did not. The changes included anastomoses among Afferent Lymph Vessels and between Afferent and efferent Lymph Vessels; proliferation of Vessels around the node; joining of parts of the capsule and trabeculae to adjacent parenchyma with loss of parts of the subcapsular and trabecular sinuses; enlargement of medullary sinuses; and reduction of the number of reticular processes in sinuses throughout the node. These changes were accompanied by a reduced ability of the node to filter chicken red blood cells labelled with chromium-51 which were injected into an Afferent Lymph Vessel.

Takuya Iida - One of the best experts on this subject based on the ideXlab platform.

  • flow oriented venous anastomosis to control Lymph flow of Lymphatic malformation
    Plastic and reconstructive surgery. Global open, 2019
    Co-Authors: Motoi Kato, Shoji Watanabe, Azusa Watanabe, Takuya Iida
    Abstract:

    : Less-invasive surgeries, such as Lymphaticovenular anastomosis (LVA), are the widely accepted intervention for Lymphedema. This study aimed to assess the outcomes of flow-oriented LVA modification on Lymphatic malformation (LM). Methods: We included 19 patients diagnosed with LM mixed type or microcystic type, who came to our clinic from June 2015 to December 2017. Under general anesthesia, all patients were administered an indocyanine green Lymphography injection subcutaneously. In the case of a strong inflow, the patient underwent Afferent Lymph Vessel of LM to venous anastomosis (LMVA). Otherwise, the side wall of LMVA was performed to the cysts. Outcomes were classified into the following groups based on the size changes: treatment effect (TE) 4 = >80% reduction rate; TE 3 = 50%-80% reduction rate; TE 2 = 20%-50% reduction rate; and TE 1 = 0%-20% reduction rate. Results: All cases underwent surgery, with no case having an increased size. The results were as follows: TE 4 = 4 (21%) patients; TE 3 = 6 (32%) patients; TE 2 = 5 (26%) patients; and TE 1 = 4 (21%) patients. No case required study termination due to disease progression. Minor complication occurred in 3 cases. One vesicle increased at the labial mucosa and one wound dehiscence that epithelized within 1 month. Conclusion: LMVA could be a novel, minimally invasive Lymph flow-oriented surgical method for intractable LM.

  • venous anastomosis procedure for treatment of Lymphatic malformation in klippel trenaunay syndrome
    Journal of pediatric surgery case reports, 2017
    Co-Authors: Motoi Kato, Shoji Watanabe, Takuya Iida, Azusa Watanabe
    Abstract:

    Abstract Klippel-Trenaunay syndrome (KTS) is a congenital vascular anomaly in children, which requires treatment but challenging. The characteristics of KTS include disproportionality of the affected limbs, usually with enlargement due to Lymphatic malformation (LM) [1]. Sometimes the condition is accompanied by pain that is difficult to treat. Compression therapy is one option for reducing the swelling; however, this has limitations [2]. We previously reported that Lymphatic venous anastomosis (LVA) may be a safe and effective procedure for reducing the size of micro cystic type LM by decreasing the intra cystic Lymph [3]. We treated a case of KTS in a 9-year-old boy who had improvement of lower abdominal swelling and penile pain after a single LVA. The method involved is to make a bypass, from the Afferent Lymph-collecting Vessel to subcutaneous veins. However, to apply LVA to LM, it is sometimes problematic to detect the Afferent flow in combined vascular malformations, such as KTS. Therefore, we performed VA making a bypass directly from LM, instead. We classify two types of LVA methods for LM: Afferent Lymph Vessel LMVA (A-LMVA), and sidewall LMVA (S-LMVA). S-LMVA has more merits than the previously reported A-LMVA.

Yoshiko Kawai - One of the best experts on this subject based on the ideXlab platform.

  • Real-time imaging of the Lymphatic channels and sentinel Lymph nodes of the stomach using contrast-enhanced ultrasonography with Sonazoid in a porcine model.
    Cancer science, 2011
    Co-Authors: Yoshiko Kawai, Kumiko Ajima, Takashi Nagai, Maki Kaidoh, Toshio Ohhashi
    Abstract:

    The contrast-enhanced ultrasound (CEUS)-guided method in combination with Sonazoid has not been clinically or experimentally evaluated with regard to its use for identifying sentinel Lymph node (SLN) in the stomach. Therefore, we attempted to evaluate the usefulness of the CEUS-guided method with Sonazoid for imaging of the Lymphatic channels and SLN of the stomach in a porcine model by comparing it with the conventional Evans blue dye-guided method. Twenty-eight 2 to 3-month-old swine weighing 17-30 kg were used in this experiment. Anesthesia was maintained with 2.0-3.0% isoflurane/O(2) inhalation. Sonazoid was injected into the intra- and sub-mucosal layers of the stomach. The intragastric or transcutaneous CEUS-guided method was used to identify the Lymphatic channels and SLN of the stomach. Contrast imaging using the CEUS-guided method with Sonazoid enabled us to produce clear images of the Afferent Lymph Vessel and SLN of the stomach until 2 h after the injection of Sonazoid. In addition, intranodal flow of the microbubble agent could be clearly identified using tissue linear harmonic images of the SLN. The SLN detection rate was not significantly different between the CEUS- and dye-guided methods. However, the Evans blue dye flowed out quickly (≈ 15 min after the injection) through the true SLN into the next LN of stomach. In conclusion, the use of the CEUS-guided method with Sonazoid might be the most useful clinical procedure for producing real-time images of the SLN of the stomach, and the linear harmonic images are also useful for evaluating intranodal structure within the SLN.

H J Spalding - One of the best experts on this subject based on the ideXlab platform.

  • effects of the adjuvant deaf dextran and staphylococcus aureus on the Lymph pathways and filtering capacity of popliteal Lymph nodes in sheep
    Research in Veterinary Science, 1991
    Co-Authors: H J Spalding, T J Heath
    Abstract:

    The effects of subcutaneous injections of the adjuvant DEAF-dextran and, or killed Staphylococcus aureus on the structure of the Lymph pathways of popliteal Lymph nodes in sheep were examined using light and electron microscopy and Microfil casts. Dextran with or without killed S aureus caused significant changes in the Lymph pathways both within the node and outside it. However, killed S aureus alone did not. The changes included anastomoses among Afferent Lymph Vessels and between Afferent and efferent Lymph Vessels; proliferation of Vessels around the node; joining of parts of the capsule and trabeculae to adjacent parenchyma with loss of parts of the subcapsular and trabecular sinuses; enlargement of medullary sinuses; and reduction of the number of reticular processes in sinuses throughout the node. These changes were accompanied by a reduced ability of the node to filter chicken red blood cells labelled with chromium-51 which were injected into an Afferent Lymph Vessel.

  • effects of the adjuvant deaf dextran and staphylococcus aureus on the Lymph pathways and filtering capacity of popliteal Lymph nodes in sheep
    Research in Veterinary Science, 1991
    Co-Authors: H J Spalding, T J Heath
    Abstract:

    The effects of subcutaneous injections of the adjuvant DEAF-dextran and, or killed Staphylococcus aureus on the structure of the Lymph pathways of popliteal Lymph nodes in sheep were examined using light and electron microscopy and Microfil casts. Dextran with or without killed S aureus caused significant changes in the Lymph pathways both within the node and outside it. However, killed S aureus alone did not. The changes included anastomoses among Afferent Lymph Vessels and between Afferent and efferent Lymph Vessels; proliferation of Vessels around the node; joining of parts of the capsule and trabeculae to adjacent parenchyma with loss of parts of the subcapsular and trabecular sinuses; enlargement of medullary sinuses; and reduction of the number of reticular processes in sinuses throughout the node. These changes were accompanied by a reduced ability of the node to filter chicken red blood cells labelled with chromium-51 which were injected into an Afferent Lymph Vessel.