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Wilhelm H Holzapfel - One of the best experts on this subject based on the ideXlab platform.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, Wilhelm H HolzapfelAbstract:ABstract Biological degradation of <B>AflatoxinB> B 1 (AFB 1 ) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB 1 was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB 1 after 48 h and only 3–6% residual AFB 1 after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14303, Nocardia coryneBacterioides DSM 12676, N. coryneBacterioides DSM 20151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44556 T were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB 1 was studied under different incuBation conditions. <B>AflatoxinB> B 1 was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12676 (formerly erroneously classified as FlavoBacterium aurantiacum ) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44556 T have shown more than 90% degradation of AFB 1 within 4 h at 30 °C, whilst after 8 h AFB 1 was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14303 and M. fluoranthenivorans sp. nov. DSM 44556 T indicate potential for application in food and feed processing.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, W H Van Zyl, Wilhelm H HolzapfelAbstract:Biological degradation of <B>AflatoxinB> B(1) (AFB(1)) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB(1) was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB(1) after 48 h and only 3-6% residual AFB(1) after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14,303, Nocardia coryneBacterioides DSM 12,676, N. coryneBacterioides DSM 20,151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44,556(T) were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB(1) was studied under different incuBation conditions. <B>AflatoxinB> B(1) was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12,676 (formerly erroneously classified as FlavoBacterium aurantiacum) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20,151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44,556(T) have shown more than 90% degradation of AFB(1) within 4 h at 30 degrees C, whilst after 8 h AFB(1) was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14,303 and M. fluoranthenivorans sp. nov. DSM 44,556(T) indicate potential for application in food and feed processing.
O D Teniola - One of the best experts on this subject based on the ideXlab platform.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, Wilhelm H HolzapfelAbstract:ABstract Biological degradation of <B>AflatoxinB> B 1 (AFB 1 ) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB 1 was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB 1 after 48 h and only 3–6% residual AFB 1 after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14303, Nocardia coryneBacterioides DSM 12676, N. coryneBacterioides DSM 20151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44556 T were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB 1 was studied under different incuBation conditions. <B>AflatoxinB> B 1 was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12676 (formerly erroneously classified as FlavoBacterium aurantiacum ) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44556 T have shown more than 90% degradation of AFB 1 within 4 h at 30 °C, whilst after 8 h AFB 1 was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14303 and M. fluoranthenivorans sp. nov. DSM 44556 T indicate potential for application in food and feed processing.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, W H Van Zyl, Wilhelm H HolzapfelAbstract:Biological degradation of <B>AflatoxinB> B(1) (AFB(1)) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB(1) was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB(1) after 48 h and only 3-6% residual AFB(1) after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14,303, Nocardia coryneBacterioides DSM 12,676, N. coryneBacterioides DSM 20,151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44,556(T) were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB(1) was studied under different incuBation conditions. <B>AflatoxinB> B(1) was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12,676 (formerly erroneously classified as FlavoBacterium aurantiacum) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20,151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44,556(T) have shown more than 90% degradation of AFB(1) within 4 h at 30 degrees C, whilst after 8 h AFB(1) was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14,303 and M. fluoranthenivorans sp. nov. DSM 44,556(T) indicate potential for application in food and feed processing.
Sarah De Saeger - One of the best experts on this subject based on the ideXlab platform.
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Mycotoxin contamination of sorghum and its contriBution to human dietary exposure in four suB-Saharan countries.
Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment, 2018Co-Authors: Geoffrey Ssepuuya, Christof Van Poucke, Emmanuel Njumbe Ediage, Catherine Mulholland, Angelika Tritscher, Mary F. Kenny, Catherine Bessy, Philippe Verger, Sarah De SaegerAbstract:This research aimed at evaluating the safety, and the type, level and prevalence of mycotoxins in grain sorghum of four suB-Saharan African (SSA) countries (Burkina Faso, Ethiopia, Mali and Sudan). A multi-analyte LC-MS/MS method for quantification of 23 mycotoxins (nivalenol, deoxynivalenol, fusarenon X, neosolaniol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, diacetoxyscirpenol, roquefortine C, HT-2 toxin, alternariol, T-2 toxin, FB1, FB2, FB3, zearalenone, <B>AflatoxinB> G(1), <B>AflatoxinB> G(2), <B>AflatoxinB> B-1, <B>AflatoxinB> B-2, sterigmatocystin, OTA, altenuene, alternariol monomethylether) was applied to different sorghum matrices. Of the 1533 analysed samples, 33% were contaminated with at least one of the following mycotoxins: <B>AflatoxinB>s, fumonisins, sterigmatocystin, Alternaria toxins, OTA and zearalenone. Country of origin, colour, source and collection period of sorghum samples significantly influenced the type, level and prevalence of mycotoxins. Sterigmatocystin (15%), fumonisins (17%) and <B>AflatoxinB>s (13%) were the most prevalent. FB1 (274 +/- 585 mu g/kg) had the highest mean concentration followed By FB2 (214 +/- 308 mu g/kg) while diacetoxyscirpenol (8.12 +/- 19.2 mu g/kg) and HT-2 (11.9 +/- 0.00 mu g/kg) had the lowest concentrations. Neosolaniol, fusarenon-X, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, T-2 toxin, nivalenol and roquefortine C were not detected in any of the samples. Sudan had the lowest prevalence and mean concentration of all mycotoxins. Pink sorghum had the highest concentrations of fumonisins and <B>AflatoxinB>s. Mycotoxins from Aspergillus spp. and Alternaria spp. are the mycotoxins of concern in SSA grain sorghum with regard to prevalence, concentration and possiBle health risk from exposure. Based on the performed risk characterisation, daily consumption of sorghum containing <B>AflatoxinB>s, alternariol, alternariol monomethyl ether, sterigmatocystin and OTA could result in exceeding the estaBlished health-Based guidance values for these toxins.
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a validated multianalyte lc ms ms method for quantification of 25 mycotoxins in cassava flour peanut cake and maize samples
Journal of Agricultural and Food Chemistry, 2011Co-Authors: Emmanuel Njumbe Ediage, Jose Diana Di Mavungu, Sofie Monbaliu, Carlos Van Peteghem, Sarah De SaegerAbstract:This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 μg/kg to 106 μg/kg. Good precision and linearity were oBserved for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the RepuBlic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. <B>AflatoxinB>s (total <B>AflatoxinB>s; 10-346 μg/kg) and ochratoxin A (
B(1) (4-21 μg/kg), <B>AflatoxinB> B(2) ( B>AflatoxinB> B(1) ( B(1) (13-836 μg/kg), fumonisin B(2) (5-221 μg/kg), fumonisin B(3) ( Beauvericin ( -
development of a multi mycotoxin liquid chromatography tandem mass spectrometry method for sweet pepper analysis
Rapid Communications in Mass Spectrometry, 2009Co-Authors: Sofie Monbaliu, Carlos Van Peteghem, Christof Van Poucke, Kris Van Poucke, Kurt Heungens, Sarah De SaegerAbstract:A multi-mycotoxin method was developed for the simultaneous determination of trichothecenes (nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, fusarenon-X, diacetoxyscirpenol, HT-2 toxin, T-2 toxin), <B>AflatoxinB>s (<B>AflatoxinB>-B-1, <B>AflatoxinB>-B-2, <B>AflatoxinB>-G(1), and <B>AflatoxinB>-G(2)), Alternaria toxins (alternariol, alternariol methyl ether and altenuene), fumonisins (fumonisin-B-1, fumonisin-B-2 and fumonisin-B-3), ochratoxin A, zearalenone, Beauvericin and sterigmatocystin in sweet pepper. Sweet pepper was extracted with ethyl acetate/formic acid (99:1, v/v). After splitting up the extract, two-thirds of the extract was cleaned up using an aminopropyl column followed By an octadecyl column. The remaining part was cleaned up using a strong anion-exchange column. After recomBination of Both cleaned parts of the sample extract, the comBined solvents were evaporated and the residue was dissolved in moBile phase; 20 mu L was injected into the chromatographic system, so only one run was used to separate and detect the mycotoxins in positive electrospray ionization using selected reaction monitoring. The samples were analyzed with a Micromass Quattro Micro triple quadrupole mass spectrometer (Waters, Milford, MA, USA). The moBile phase consisted of variaBle mixtures of water and methanol, 1% acetic acid and 5mM ammonium acetate. The limits of detection of the multi-mycotoxin method varied from 0.32 mu g.kg(-1) to 42.48 mu g.kg(-1). The multi-mycotoxin liquid chromatography/tandem mass spectrometry (LC/MS/MS) method fulfilled the method performance criteria required By the Commission Regulation (EC) No 401/2006. Sweet peppers inoculated By Fusarium species were analyzed using the developed method. Beauvericin (9-484 mu g.kg(-1)) and fumonisins (fumonisin-B-1 up to 4330 mu g.kg(-1), fumonisin-B-2 up to 4900 mu g.kg(-1), and fumonisin-B-3 up to 299 mu g.kg(-1)) were detected.
P S Steyn - One of the best experts on this subject based on the ideXlab platform.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, Wilhelm H HolzapfelAbstract:ABstract Biological degradation of <B>AflatoxinB> B 1 (AFB 1 ) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB 1 was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB 1 after 48 h and only 3–6% residual AFB 1 after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14303, Nocardia coryneBacterioides DSM 12676, N. coryneBacterioides DSM 20151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44556 T were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB 1 was studied under different incuBation conditions. <B>AflatoxinB> B 1 was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12676 (formerly erroneously classified as FlavoBacterium aurantiacum ) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44556 T have shown more than 90% degradation of AFB 1 within 4 h at 30 °C, whilst after 8 h AFB 1 was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14303 and M. fluoranthenivorans sp. nov. DSM 44556 T indicate potential for application in food and feed processing.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, W H Van Zyl, Wilhelm H HolzapfelAbstract:Biological degradation of <B>AflatoxinB> B(1) (AFB(1)) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB(1) was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB(1) after 48 h and only 3-6% residual AFB(1) after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14,303, Nocardia coryneBacterioides DSM 12,676, N. coryneBacterioides DSM 20,151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44,556(T) were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB(1) was studied under different incuBation conditions. <B>AflatoxinB> B(1) was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12,676 (formerly erroneously classified as FlavoBacterium aurantiacum) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20,151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44,556(T) have shown more than 90% degradation of AFB(1) within 4 h at 30 degrees C, whilst after 8 h AFB(1) was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14,303 and M. fluoranthenivorans sp. nov. DSM 44,556(T) indicate potential for application in food and feed processing.
P Addo - One of the best experts on this subject based on the ideXlab platform.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, Wilhelm H HolzapfelAbstract:ABstract Biological degradation of <B>AflatoxinB> B 1 (AFB 1 ) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB 1 was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB 1 after 48 h and only 3–6% residual AFB 1 after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14303, Nocardia coryneBacterioides DSM 12676, N. coryneBacterioides DSM 20151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44556 T were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB 1 was studied under different incuBation conditions. <B>AflatoxinB> B 1 was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12676 (formerly erroneously classified as FlavoBacterium aurantiacum ) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44556 T have shown more than 90% degradation of AFB 1 within 4 h at 30 °C, whilst after 8 h AFB 1 was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14303 and M. fluoranthenivorans sp. nov. DSM 44556 T indicate potential for application in food and feed processing.
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degradation of <B>AflatoxinB> B1 By cell free extracts of rhodococcus erythropolis and mycoBacterium fluoranthenivorans sp nov dsm44556t
International Journal of Food Microbiology, 2005Co-Authors: O D Teniola, P Addo, I Brost, P Farber, K D Jany, J F Alberts, P S Steyn, W H Van Zyl, Wilhelm H HolzapfelAbstract:Biological degradation of <B>AflatoxinB> B(1) (AFB(1)) By Rhodococcus erythropolis was examined in liquid cultures and in cell-free extracts. Dramatic reduction of AFB(1) was oBserved during incuBation in the presence of R. erythropolis cells (17% residual AFB(1) after 48 h and only 3-6% residual AFB(1) after 72 h). Cell-free extracts of four Bacterial strains, R. erythropolis DSM 14,303, Nocardia coryneBacterioides DSM 12,676, N. coryneBacterioides DSM 20,151, and MycoBacterium fluoranthenivorans sp. nov. DSM 44,556(T) were produced By disrupting cells in a French pressure cell. The aBility of crude cell-free extracts to degrade AFB(1) was studied under different incuBation conditions. <B>AflatoxinB> B(1) was effectively degraded By cell free extracts of all four Bacterial strains. N. coryneBacterioides DSM 12,676 (formerly erroneously classified as FlavoBacterium aurantiacum) showed the lowest degradation aBility (60%) after 24 h, while >90% degradation was oBserved with N. coryneBacterioides DSM 20,151 over the same time. R. erythropolis and M. fluoranthenivorans sp. nov. DSM 44,556(T) have shown more than 90% degradation of AFB(1) within 4 h at 30 degrees C, whilst after 8 h AFB(1) was practicaBly not detectaBle. The high degradation rate and wide temperature range for degradation By R. erythropolis DSM 14,303 and M. fluoranthenivorans sp. nov. DSM 44,556(T) indicate potential for application in food and feed processing.