The Experts below are selected from a list of 23253 Experts worldwide ranked by ideXlab platform
J P Dubey - One of the best experts on this subject based on the ideXlab platform.
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direct Agglutination Test for the detection of antibodies to sarcocystis neurona in experimentally infected animals
Veterinary Parasitology, 2001Co-Authors: J P DubeyAbstract:Equine protozoal myeloencephalitis (EPM) is a serious neurological disease of horses in the Americas. The apicomplexan protozoan most commonly associated with EPM is Sarcocystis neurona. A direct Agglutination Test (SAT) was developed to detect antibodies to S. neurona in experimentally infected animals. Merozoites of the SN6 strain of S. neurona collected from cell culture were used as antigen and 2-mercaptoethanol was added to the antigen suspension to destroy IgM antibodies when mixed with Test sera. Mice fed sporocysts of S. speeri or S. falcatula-like sporocysts from opossums did not seroconvert in the SAT. The sensitivity of the SAT was 100% and the specificity was 90% in mice.
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serosurvey of toxoplasma gondii in north atlantic marine mammals by the use of Agglutination Test employing whole tachyzoites and dithiothreitol
Comparative Immunology Microbiology and Infectious Diseases, 1998Co-Authors: Antti Oksanen, Morten Tryland, K Johnsen, J P DubeyAbstract:Abstract Serum samples from North Atlantic populations of harp seal, Phoca groenlandica (n=316), ringed seal, Phoca hispida (n=48), hooded seal, Cystophora cristata (n=78), and minke whale, Balaenoptera acutorostrata (n=202), were Tested for Toxoplasma gondii-specific IgG. The modified Agglutination Test (MAT) was slightly modified to be more user-friendly by replacing the 0.2 M 2-mercaptoethanol with 10 mM dithiothreitol. No positive samples were found at 1:40 dilution.
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direct Agglutination Test for serologic diagnosis of neospora caninum infection
Parasitology Research, 1997Co-Authors: Stephane Romand, P Thulliez, J P DubeyAbstract:A direct Agglutination Test was evaluated for the detection and quantitation of IgG antibodies to Neospora caninum in both experimental and natural infections in various animal species. As compared with results obtained by the indirect fluorescent antibody Test, the direct Agglutination Test appeared reliable for the serologic diagnosis of neosporosis in a variety of animal species. The direct Agglutination Test should provide easily available and inexpensive tools for serologic Testing for antibodies to N. caninum in many host species.
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validation of the specificity of the modified Agglutination Test for toxoplasmosis in pigs
Veterinary Parasitology, 1997Co-Authors: J P DubeyAbstract:Serum samples from Toxoplasma gondii-negative pigs infected with protozoa, helminths and viruses, and from fetal pigs were examined for cross reacting antibodies to T. gondii by the use of the modified Agglutination Test (MAT). Nonspecific reactions with T. gondii antigen were not found in 1:10 dilutions of sera from eight pigs experimentally infected with Sarcocystis miescheriana, 11 pigs infected with Ascaris suum, three pigs infected with Trichuris suis, ten pigs infected with Trichinella spiralis, nine pigs infected with various porcine viruses, six gnotobiotic pigs, and 82 fetal pigs. Antibodies were also not found in 1:25 dilution of sera from 200 naturally aborted fetal pigs. Thus, evidence for cross reactivity to T. gondii with unrelated or related organisms using the MAT was not found.
D W Warnock - One of the best experts on this subject based on the ideXlab platform.
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use of the pastorex aspergillus antigen latex Agglutination Test for the diagnosis of invasive aspergillosis
Journal of Clinical Pathology, 1995Co-Authors: V Hopwood, Jacqueline M Cornish, A B M Foot, E G V Evans, Elizabeth M Johnson, D W WarnockAbstract:AIMS--To evaluate the Pastorex aspergillus antigen latex Agglutination Test for the diagnosis of invasive aspergillosis in patients undergoing liver or bone marrow transplantation. METHODS--Serum samples were taken at least twice weekly post-transplant and Tested for Aspergillus antigen. Latex Agglutination Test results were compared with microbiological examination of respiratory, urine and bile specimens. Serum samples from liver transplant patients were also Tested for antibodies to Aspergillus fumigatus by counter immunoelectrophoresis. RESULTS--Eight of the 91 patients studied developed invasive aspergillosis. Positive latex Agglutination Tests were obtained in eight of 187 (4.3%) serum samples from four of these eight patients. The other four patients with invasive aspergillosis gave consistently negative latex Agglutination Tests. A positive latex Agglutination Test was the first indication of invasive aspergillosis in two patients; these patients were already on amphotericin B. Positive latex Agglutination Tests were the only evidence of invasive aspergillosis in one patient who subsequently died of the infection. False positive latex Agglutination Tests were obtained in five of 83 (6%) patients with no evidence of invasive aspergillosis and misleading positive cultures seen in nine of 83 (10.8%). No antibodies were detected in three of four liver transplant patients with invasive aspergillosis. Conversely, antibodies were detected in 63 of 262 (24%) serum samples from 43 liver transplant patients with no evidence of invasive aspergillosis; one of these patients had an antibody titre of 1:2 on four separate occasions. CONCLUSIONS--The Pastorex aspergillus antigen latex Agglutination Test, when used alone, lacks sensitivity and specificity for the early diagnosis of invasive aspergillosis. A diagnosis was made in all patients with invasive aspergillosis when both culture and antigen Tests were performed although using these criteria a false positive diagnosis would have been made in 13 of 83 (15.6%) patients. Microbiological and serial serological investigations for antigen should both be performed and the results considered in conjunction with radiological and clinical evidence.
Bradd C Barr - One of the best experts on this subject based on the ideXlab platform.
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a modified Agglutination Test for neospora caninum development optimization and comparison to the indirect fluorescent antibody Test and enzyme linked immunosorbent assay
Clinical and Vaccine Immunology, 1998Co-Authors: Andrea E Packham, Karen W Sverlow, Patricia A Conrad, Emily F Loomis, Joan D Rowe, Mark L Anderson, Antoinette E Marsh, Carolyn Cray, Bradd C BarrAbstract:Current serologic Tests used to detect antibodies to Neospora caninum require species-specific secondary antibodies, limiting the number of species that can be Tested. In order to examine a wide variety of animal species that may be infected with N. caninum, a modified direct Agglutination Test (N-MAT) similar to the Toxoplasma gondii modified direct Agglutination Test (T-MAT) was developed. This Test measures the direct Agglutination of parasites by N. caninum-specific antibodies in serum, thus eliminating the need for secondary host-specific anti-isotype sera. The N-MAT was compared to the indirect fluorescent-antibody Test (IFAT) and the enzyme-linked immunosorbent assay (ELISA) with a “gold standard” serum panel from species for which secondary antibodies were available (n = 547). All positive samples Tested were from animals with histologically confirmed infections. Up to 16 different species were Tested. The N-MAT gave a higher sensitivity (100%) and specificity (97%) than the ELISA (74 and 94%, respectively) and had a higher sensitivity but a lower specificity than the IFAT (98 and 99%, respectively). The reduced specificity of the N-MAT was due to false-positive reactions in Testing fetal fluids with particulate matter or severely hemolyzed serum. Overall, the N-MAT proved to be highly sensitive and specific for both naturally and experimentally infected animals, highly reproducible between and within readers, easy to use on large sample sizes without requiring special equipment, and useful in Testing serum from any species without modification.
V Hopwood - One of the best experts on this subject based on the ideXlab platform.
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use of the pastorex aspergillus antigen latex Agglutination Test for the diagnosis of invasive aspergillosis
Journal of Clinical Pathology, 1995Co-Authors: V Hopwood, Jacqueline M Cornish, A B M Foot, E G V Evans, Elizabeth M Johnson, D W WarnockAbstract:AIMS--To evaluate the Pastorex aspergillus antigen latex Agglutination Test for the diagnosis of invasive aspergillosis in patients undergoing liver or bone marrow transplantation. METHODS--Serum samples were taken at least twice weekly post-transplant and Tested for Aspergillus antigen. Latex Agglutination Test results were compared with microbiological examination of respiratory, urine and bile specimens. Serum samples from liver transplant patients were also Tested for antibodies to Aspergillus fumigatus by counter immunoelectrophoresis. RESULTS--Eight of the 91 patients studied developed invasive aspergillosis. Positive latex Agglutination Tests were obtained in eight of 187 (4.3%) serum samples from four of these eight patients. The other four patients with invasive aspergillosis gave consistently negative latex Agglutination Tests. A positive latex Agglutination Test was the first indication of invasive aspergillosis in two patients; these patients were already on amphotericin B. Positive latex Agglutination Tests were the only evidence of invasive aspergillosis in one patient who subsequently died of the infection. False positive latex Agglutination Tests were obtained in five of 83 (6%) patients with no evidence of invasive aspergillosis and misleading positive cultures seen in nine of 83 (10.8%). No antibodies were detected in three of four liver transplant patients with invasive aspergillosis. Conversely, antibodies were detected in 63 of 262 (24%) serum samples from 43 liver transplant patients with no evidence of invasive aspergillosis; one of these patients had an antibody titre of 1:2 on four separate occasions. CONCLUSIONS--The Pastorex aspergillus antigen latex Agglutination Test, when used alone, lacks sensitivity and specificity for the early diagnosis of invasive aspergillosis. A diagnosis was made in all patients with invasive aspergillosis when both culture and antigen Tests were performed although using these criteria a false positive diagnosis would have been made in 13 of 83 (15.6%) patients. Microbiological and serial serological investigations for antigen should both be performed and the results considered in conjunction with radiological and clinical evidence.
Hedayat Shirzad - One of the best experts on this subject based on the ideXlab platform.
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development of a latex Agglutination Test as a simple and rapid method for diagnosis of trichomonas vaginalis infection
Avicenna journal of medical biotechnology, 2010Co-Authors: Hossein Yousofi Darani, Firuzeh Ahmadi, Nozhat Zabardast, Hossein Ali Yousefi, Hedayat ShirzadAbstract:Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex Agglutination Test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and latex Agglutination Tests. Sensitivity and specificity of latex Test was determined considering culture as golden standard. Sensitivity and specificity of latex Agglutination Test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of latex Agglutination Test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the latex Agglutination Test developed in this study, further work is recommended to improve the Test.