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Gejiao Wang - One of the best experts on this subject based on the ideXlab platform.

Phillip Mullineaux - One of the best experts on this subject based on the ideXlab platform.

  • Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Mahmoud A. Hassan, Harry Jan Swartz, Gordon Inamine, Phillip Mullineaux
    Abstract:

    Experiments in shoot regeneration and virulent Agrobacterium tumefaciens -mediated transformation were used to develop a protocol for Rubus transformation. This protocol was then used to produce transformed Rubus plants from in vitro internodes inoculated with an Agrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml^−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.

  • Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Mahmoud A. Hassan, Harry Jan Swartz, Gordon Inamine, Phillip Mullineaux
    Abstract:

    Experiments in shoot regeneration and virulent Agrobacterium tumefaciens -mediated transformation were used to develop a protocol for Rubus transformation. This protocol was then used to produce transformed Rubus plants from in vitro internodes inoculated with an Agrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml^−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.

Zixu Qiao - One of the best experts on this subject based on the ideXlab platform.

Lan-ying Lee - One of the best experts on this subject based on the ideXlab platform.

  • Integration of genes into the chromosome of Agrobacterium tumefaciens C58.
    Methods in molecular biology (Clifton N.J.), 2006
    Co-Authors: Lan-ying Lee
    Abstract:

    Agrobacterium tumefaciens has been widely used to transform numerous plant species. Frequently, investigators want to place more than one gene into Agrobacterium in order to manipulate various bacterial functions during plant genetic transformation. These genes are frequently brought into the bacterium by multiple plasmids. It is difficult to maintain several plasmids in the same bacterium without the use of a variety of antibiotics. However, the use of some antibiotics is not feasible for certain Agrobacterium strains. Also, too many different antibiotics in the culture medium generally results in slow growth of Agrobacterium. Therefore, it is to one's advantage to place genes of interest onto the bacterial chromosome to eliminate the use of multiple antibiotics during bacterial growth. This chapter describes in detail a method to integrate a gene of interest into the pgl/picA locus of the Agrobacterium tumefaciens C58 chromosome. The integrated gene is stably maintained as single copy per cell without the need for selection.

Mahmoud A. Hassan - One of the best experts on this subject based on the ideXlab platform.

  • Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Mahmoud A. Hassan, Harry Jan Swartz, Gordon Inamine, Phillip Mullineaux
    Abstract:

    Experiments in shoot regeneration and virulent Agrobacterium tumefaciens -mediated transformation were used to develop a protocol for Rubus transformation. This protocol was then used to produce transformed Rubus plants from in vitro internodes inoculated with an Agrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml^−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.

  • Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Mahmoud A. Hassan, Harry Jan Swartz, Gordon Inamine, Phillip Mullineaux
    Abstract:

    Experiments in shoot regeneration and virulent Agrobacterium tumefaciens -mediated transformation were used to develop a protocol for Rubus transformation. This protocol was then used to produce transformed Rubus plants from in vitro internodes inoculated with an Agrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml^−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.