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Agrobacterium tumefaciens

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Gejiao Wang – One of the best experts on this subject based on the ideXlab platform.

  • Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4.
    Scientific Reports, 2017
    Co-Authors: Zixu Qiao, Timothy R. Mcdermott, Qian Wang, Gejiao Wang

    Abstract:

    Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4

Phillip Mullineaux – One of the best experts on this subject based on the ideXlab platform.

  • Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Mahmoud A. Hassan, Harry Jan Swartz, Gordon Inamine, Phillip Mullineaux

    Abstract:

    Experiments in shoot regeneration and virulent Agrobacterium tumefaciens -mediated transformation were used to develop a protocol for Rubus transformation. This protocol was then used to produce transformed Rubus plants from in vitro internodes inoculated with an Agrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml^−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.

  • Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants
    Plant Cell Tissue and Organ Culture, 1993
    Co-Authors: Mahmoud A. Hassan, Harry Jan Swartz, Gordon Inamine, Phillip Mullineaux

    Abstract:

    Experiments in shoot regeneration and virulent Agrobacterium tumefaciens -mediated transformation were used to develop a protocol for Rubus transformation. This protocol was then used to produce transformed Rubus plants from in vitro internodes inoculated with an Agrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml^−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.

Zixu Qiao – One of the best experts on this subject based on the ideXlab platform.

  • Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4.
    Scientific Reports, 2017
    Co-Authors: Zixu Qiao, Timothy R. Mcdermott, Qian Wang, Gejiao Wang

    Abstract:

    Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4