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Woojun Park - One of the best experts on this subject based on the ideXlab platform.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
Hyerim Hong - One of the best experts on this subject based on the ideXlab platform.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
Aldert Bart - One of the best experts on this subject based on the ideXlab platform.
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Plasmid diversity in neisseriae.
Infection and Immunity, 2006Co-Authors: Mark Wj Van Passel, Arie Van Der Ende, Aldert BartAbstract:Horizontal gene transfer constitutes an important force in prokaryotic genome evolution, and it is well-known that Plasmids are vehicles for DNA transfer. Chromosomal DNA is frequently exchanged between pathogenic and commensal neisseriae, but relatively little is known about plasmid diversity and prevalence among these nasopharyngeal inhabitants. We investigated the plasmid contents of 18 Neisseria lactamica isolates and 20 nasopharyngeal Neisseria meningitidis isolates. Of 18 N. lactamica strains, 9 harbored one or more Plasmids, whereas only one N. meningitidis isolate contained a plasmid. Twelve Plasmids were completely sequenced, while five plasmid sequences from the public databases were also included in the analyses. On the basis of nucleic acid sequences, mobilization, and replicase protein alignments, we distinguish six different plasmid groups (I to VI). Three Plasmids from N. lactamica appeared to be highly similar on the nucleotide level to the meningococcal Plasmids pJS-A (>99%) and pJS-B (>75%). The genetic organizations of two Plasmids show a striking resemblance with that of the recently identified meningococcal disease-associated (MDA) phage, while four putative proteins encoded by these Plasmids show 25% to 39% protein identity to those encoded by the MDA phage. The putative promoter of the gene encoding the replicase on these Plasmids contains a polycytidine tract, suggesting that replication is subjected to phase variation. In conclusion, extensive plasmid diversity is encountered among commensal neisseriae. Members of three plasmid groups are found in both pathogenic and commensal neisseriae, indicating plasmid exchange between these species. Resemblance between Plasmids and MDA phage may be indicative of dissemination of phage-related sequences among pathogenic and commensal neisseriae.
Hyeok-jin Ko - One of the best experts on this subject based on the ideXlab platform.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
In-geol Choi - One of the best experts on this subject based on the ideXlab platform.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.
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Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
Microbial Ecology, 2014Co-Authors: Hyerim Hong, Hyeok-jin Ko, In-geol Choi, Woojun ParkAbstract:We used culture-dependent and culture-independent methods to extract previously undescribed Plasmids harboring tetracycline (TC) resistance genes from activated sludge. The extracted Plasmids were transformed into naturally competent Acinetobacter oleivorans DR1 to recover a non- Escherichia coli -based plasmid. The transformed cells showed 80–100-fold higher TC resistance than the wild-type strain. Restriction length polymorphism performed using 30 transformed cells showed four different types of Plasmids. Illumina-based whole sequencing of the four Plasmids identified three previously unreported Plasmids and one previously reported plasmid. All Plasmids carried TC resistance-related genes ( tetL , tetH ), tetracycline transcriptional regulators ( tetR ), and mobilization-related genes. As per expression analysis, TC resistance genes were functional in the presence of TC. The recovered Plasmids showed mosaic gene acquisition through horizontal gene transfer. Membrane fluidity, hydrophobicity, biofilm formation, motility, growth rate, sensitivity to stresses, and quorum sensing signals of the transformed cells were different from those of the wild-type cells. Plasmid-bearing cells seemed to have an energy burden for maintaining and expressing plasmid genes. Our data showed that acquisition of TC resistance through plasmid uptake is related to loss of biological fitness. Thus, cells acquiring antibiotic resistance Plasmids can survive in the presence of antibiotics, but must pay ecological costs.