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Ajellomyces

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Lynne Sigler – 1st expert on this subject based on the ideXlab platform

  • The Ajellomycetaceae, a new family of vertebrate-associated Onygenales
    Mycologia, 2020
    Co-Authors: Wendy A. Untereiner, Lynne Sigler, James A. Scott, Françoise A. Naveau, Jason Bachewichj. Bachewich, Andrea Angusa. Angus

    Abstract:

    Phylogenies inferred from the analysis of DNA sequence data have shown that the Onygenales contains clades that do not correspond with previously described families. One lineage identified in recent molecular phylogenetic studies includes the dimorphic pathogens belonging to the genera Ajellomyces, Emmonsia and Paracoccidioides. To evaluate the degree of support for this lineage and determine whether it includes additional taxa, we examined relationships among the members of this clade and selected saprobic onygenalean taxa based on maximum-parsimony analyses of partial nuclear large RNA subunit (LSU) and internal transcribed spacer (ITS) sequences. A clade distinct from the Onygenaceae was found to encompass Ajellomyces (including the anamorph genera Blastomyces, Emmonsia and Histoplasma) and Paracoccidioides brasiliensis. The members of this lineage are saprobic and pathogenic vertebrate-associated taxa distinguished by their globose ascomata with coiled appendages, muricate globose or oblate ascospores…

  • molecular genetic variation in emmonsia crescens and emmonsia parva etiologic agents of adiaspiromycosis and their phylogenetic relationship to blastomyces dermatitidis Ajellomyces dermatitidis and other systemic fungal pathogens
    Journal of Clinical Microbiology, 1998
    Co-Authors: Stephen W Peterson, Lynne Sigler

    Abstract:

    Emmonsia crescens, an agent of adiaspiromycosis, Blastomyces dermatitidis, the agent of blastomycosis, and Histoplasma capsulatum, the agent of histoplasmosis, are known to form meiotic (sexual) stages in the ascomycete genus Ajellomyces (Onygenaceae, Onygenales), but no sexual stage is known for E. parva, the type species of the genus Emmonsia. To evaluate relationships among members of the putative Ajellomyces clade, large-subunit ribosomal and internal transcribed spacer region DNA sequences were determined from PCR-amplified DNA fragments. Sequences were analyzed phylogenetically to evaluate the genetic variation within the genus Emmonsia and evolutionary relationships to other taxa. E. crescens and E. parva are distinct species. E. crescens isolates are placed into two groups that correlate with their continents of origin. Considerable variation occurred among isolates previously classified as E. parva. Most isolates are placed into two closely related groups, but the remaining isolates, including some from human sources, are phylogenetically distinct and represent undescribed species. Strains of B. dermatitidis are a sister species of E. parva. Paracoccidioides brasiliensis and Histoplasma capsulatum are ancestral to most Emmonsia isolates, and P. brasiliensis, which has no known teleomorph, falls within the Ajellomyces clade.

  • Ajellomyces crescens sp nov taxonomy of emmonsia spp and relatedness with blastomyces dermatitidis teleomorph Ajellomyces dermatitidis
    Medical Mycology, 1996
    Co-Authors: Lynne Sigler

    Abstract:

    Adiaspiromycosis is known primarily as a pulmonary infection of small burrowing mammals and rarely of humans, in which the tissue spore form consists of a large, globose, thick-walled, non-proliferating structure called an adiaspore. The causative agents have been placed in Emmonsia or Chrysosporium and treated as either two species or varieties. Emmonsia parva (= Chrysosporium parvum var. parvum) has been distinguished from E. crescens (= C. parvum var. crescens) by differences in maximum growth temperature, size of adiaspores, host range and geographical distribution. Phenotypic similarities between Emmonsia spp. and Blastomyces dermatitidis and chance observation of Ajellomyces-type ascomatal hyphae led to the hypothesis that the teleomorph of Emmonsia spp. could occur in Ajellomyces. Isolates preliminarily identified as E. parva or E. crescens were examined by morphology and physiology and tested for compatibility in mating experiments. Ajellomyces crescens Sigler sp. nov. is described for the teleomo…

D H Pincus – 2nd expert on this subject based on the ideXlab platform

  • biotyping method for Ajellomyces dermatitidis enzymatic activity profiling as a potential
    , 2013
    Co-Authors: R C Summerbell, J Kane, D H Pincus

    Abstract:

    aminopeptidase; TR, tryptophan aminopeptidase; HP, hydroxyprolineaminopeptidase; IL, isoleucine aminopeptidase; VA, valine aminopeptidase; LU, leucyl glycine aminopeptidase; HI, histidine aminopeptidase; CY, cystineaminopeptidase; TY,tyrosine aminopeptidase; GLG,glycylglycine aminopeptidase. Symbols: +, positive; -, negative; v, reactionvariablebetweenreplicates.

  • enzymatic activity profiling as a potential biotyping method for Ajellomyces dermatitidis
    Journal of Clinical Microbiology, 1990
    Co-Authors: R C Summerbell, J Kane, D H Pincus

    Abstract:

    Enzyme profiling was investigated as a means of recognizing biotypes and individual strains of Ajellomyces dermatitidis (anamorph, Blastomyces dermatitidis). Eighteen North American and 2 African representatives were tested with the Yeast-IDENT enzymatic activity profiling system (Analytab Products, Plainview, N.Y.). Significant variation was found between isolates, particularly in beta-galactosaminidase activity.

Takashi Mikawa – 3rd expert on this subject based on the ideXlab platform

  • Phylogenetic analysis of the non-pathogenic genusSpiromastix (Onygenaceae) and related onygenalean taxa based on large subunit ribosomal DNA sequences
    Mycoscience, 2001
    Co-Authors: Masato Sugiyama, Takashi Mikawa

    Abstract:

    The phylogenetic positioning of the non-pathogenic genus Spiromastix in the Onygenales was studied based on large subunit rDNA (LSU rDNA) partial sequences (ca. 570 bp.). Four Spiromastix species and 28 representative taxa of the Onygenales were newly sequenced. Phylogenetic trees were constructed by the neighbor-joining (NJ) method and evaluated by the maximum parsimony (MP) method with the data of 13 taxa retrieved from DNA databases. Spiromastix and dimorphic systemic pathogens, Ajellomyces and Paracoccidioides , appear to be a monophyletic group with 74% bootstrap probability (BP) in the NJ tree constructed with the representative taxa of the Onygenales. The tree topology was concordant with the NJ tree based on SSU rDNA sequences of our previous work and corresponded to the classification system of the Onygenales by Currah (1985) and its minor modification by Udagawa (1997) with the exception of the classification of the Onygenaceae. The Onygeneceae sensu Udagawa may still be polyphyletic, since three independent lineages were recognized. The taxa forming helicoid peridial appendages were localized to two clades on the tree. The topology of the NJ tree constructed with Spiromastix and its close relatives suggested that the helicoid peridial appendages were apomorphic and acquired independently in the two clades of the Onygenales.

  • Phylogenetic analysis of the non-pathogenic genus Spiromastix (Onygenaceae) and related onygenalean taxa based on large subunit ribosomal DNA sequences
    Mycoscience, 2001
    Co-Authors: Masato Sugiyama, Takashi Mikawa

    Abstract:

    The phylogenetic positioning of the non-pathogenic genusSpiromastix in the Onygenales was studied based on large subunit rDNA (LSU rDNA) partial sequences (ca. 570 bp.). FourSpiromastix species and 28 representative taxa of the Onygenales were newly sequenced. Phylogenetic trees were constructed by the neighbor-joining (NJ) method and evaluated by the maximum parsimony (MP) method with the data of 13 taxa retrieved from DNA databases.Spiromastix and dimorphic systemic pathogens,Ajellomyces andParacoccidioides, appear to be a monophyletic group with 74% bootstrap probability (BP) in the NJ tree constructed with the representative taxa of the Onygenales. The tree topology was concordant with the NJ tree based on SSU rDNA sequences of our previous work and corresponded to the classification system of the Onygenales by Currah (1985) and its minor modification by Udagawa (1997) with the exception of the classification of the Onygenaceae. The Onygeneceae sensu Udagawa may still be polyphyletic, since three independent lineages were recognized. The taxa forming helicoid peridial appendages were localized to two clades on the tree. The topology of the NJ tree constructed withSpiromastix and its close relatives suggested that the helicoid peridial appendages were apomorphic and acquired independently in the two clades of the Onygenales.

  • Molecular phylogeny of onygenalean fungi based on small subunit ribosomal DNA (SSU rDNA) sequences
    Mycoscience, 1999
    Co-Authors: Masato Sugiyama, Akiko Ohara, Takashi Mikawa

    Abstract:

    Phylogenetic analysis of nucleotide data from small subunit ribosomal DNA (SSU rDNA) sequences (ca. 1685 bp.) was performed on 19 taxa of the Onygenales and three related mitosporic fungi. Phylogenetic trees were constructed by the neighbor-joining method with the sequence data of related taxa obtained from DNA databases. The species in the Onygenales form two clusters and seven subclusters, and the tree topology reflects the traditional classification by Currah (1985) with some exceptions. The Myxotrichaceae is placed in the different lineage, separate from other plectomycetous taxa and among the Leotiales and the Erysiphales. Furthermore, two separate lineages in the Myxotrichaceae were found. Tree topology suggested the Onygenaceae is polyphyletic and composed of three subgroups; 1) most members of Onygenaceae, 2) Spiromastix warcupii , and 3) pathogenic dimorphic fungi classified in Ajellomyces .