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Hubert Ferte - One of the best experts on this subject based on the ideXlab platform.
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MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea)
Parasite, 2019Co-Authors: Antoine Huguenin, Jérôme Depaquit, Isabelle Villena, Hubert FerteAbstract:Identification of cercariae was long based on morphological and morphometric features, but these approaches remain difficult to implement and require skills that have now become rare. Molecular tools have become the reference even though they remain relatively time-consuming and expensive. We propose a new approach for the identification of cercariae using MALDI-TOF mass spectrometry. Snails of different genera (Radix, Lymnaea, Stagnicola, Planorbis, and Anisus) were collected in the field to perform emitting tests in the laboratory. The cercariae they emitted (Trichobilharzia anseri, Diplostomum pseudospathaceum, Alaria alata, Echinostoma revolutum, Petasiger phalacrocoracis, Tylodelphys sp., Australapatemon sp., Cotylurus sp., Posthodiplostomum sp., Parastrigea sp., Echinoparyphium sp. and Plagiorchis sp.) were characterized by sequencing the D2, ITS2 and ITS1 domains of rDNA, and by amplification using specific Alaria alata primers. A sample of each specimen, either fresh or stored in ethanol, was subjected to a simple preparation protocol for MALDI-TOF analysis. The main spectral profiles were analyzed by Hierarchical Clustering Analysis. Likewise, the haplotypes were analyzed using the maximum likelihood method. Analytical performance and the log-score value (LSV) cutoff for species identification were then assessed by blind testing. The clusters obtained by both techniques were congruent, allowing identification at a species level. MALDI-TOF enables identification at an LSV cutoff of 1.7 without false-positives; however, it requires more data on closely related species. The development of a "high throughput" identification system for all types of cercariae would be of considerable interest in epidemiological surveys of trematode infections.
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detection of planorbis planorbis and anisus vortex as first intermediate hosts of Alaria alata goeze 1792 in natural conditions in france molecular evidence
Veterinary Parasitology, 2012Co-Authors: Julien Portier, Damien Jouet, Isabelle Vallee, Hubert FerteAbstract:Alaria alata (Goeze, 1792), a trematode that parasitizes canids, usually needs two intermediate hosts to complete its life cycle: an aquatic freshwater snail and an amphibian. Although many studies have been undertaken on the wild boar's role as paratenic host, owing to the potential threat to human health, few have sought to identify the snails that act as first intermediate hosts in natural conditions. Adopting a molecular approach, with specific markers for a portion of the second internal transcribed spacer (ITS-2), we detected haplotypes of A. alata furcocercariae in two snail species (Planorbis planorbis and Anisus vortex), identified by molecular analysis (ribosomal 18S, mitochondrial 16S and COI). This study provides the first description of snails naturally emitting A. alata furcocercaria in Western Europe.
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new data in france on the trematode Alaria alata goeze 1792 obtained during trichinella inspections
Parasite, 2011Co-Authors: Julien Portier, Damien Jouet, Hubert Ferte, O Gibout, A Heckmann, Pascal Boireau, Isabelle ValleeAbstract:The trematode Alaria alata is a cosmopolite parasite found in red foxes (Vulpes vulpes), the main definitive host in Europe. In contrast only few data are reported in wild boars (Sus scrofa), a paratenic host. The aim of this paper is to describe the importance and distribution of Alaria alata mesocercariae in wild boars, information is given by findings of these larvae during Trichinella mandatory meat inspection on wild boars' carcasses aimed for human consumption. More than a hundred cases of mesocercariae positive animals are found every year in the East of France. First investigations on the parasite's resistance to deep-freezing in meat are presented in this work.
Michael Y. Roleda - One of the best experts on this subject based on the ideXlab platform.
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In vitro evaluation of utilisable crude protein and methane production for a diet in which grass silage was replaced by different levels and fractions of extracted seaweed proteins
Animal Feed Science and Technology, 2019Co-Authors: Maryam Ramin, Inga Marie Aasen, Michael Y. Roleda, Marcia De Oliveira Franco, Marten Hetta, Håvard SteinshamnAbstract:Abstract Utilisable crude protein (uCP), methane (CH 4 ) production and other fermentation parameters were analysed in vitro for a diet in which grass silage was replaced by different levels of seaweed protein fractions prepared from three seaweed species: Saccharina latissima , Alaria esculenta and Palmaria palmata . Ten fractions from these three species in which the protein content had been increased and the salt content reduced by simple processing were tested, with inclusion levels in the diet based on the nitrogen content of the fractions. Following an extraction procedure, four fractions from Saccharina latissima , three from Alaria esculenta and one from Palmaria palmata , were gradually included in the diet by replacing high quality silage with approximately 0, 0.15, 0.30 and 0.45 g/g DM, while two high-protein fractions of Palmaria palmata were tested at replacement levels of 0, 0.075, 0.15 and 0.225 g/g DM. To estimate fermentation parameters, 500 mg of each diet were incubated in bottles with 60 mL buffered rumen fluid. Estimated uCP increased linearly with increasing replacement rate of grass silage with seaweed protein fractions (from 158 g/kg DM to 206 g/kg DM on average for all fractions). Increasing protein fraction from the brown seaweed Saccharina latissima in the diet significantly increased true organic matter digestibility (OMD) (from on average 0.786 to 0.821). Organic matter digestibility decreased with increasing level of Alaria esculenta fractions (from on average 0.785 to 0.733), which also gave a linear decrease in CH 4 production (from on average 45.3 to 38.5 mL/g organic matter). As a result of decreased CH 4 production and OMD, total volatile fatty acid concentration decreased with increasing level of Alaria esculenta fractions (from on average 69.5 to 63.0 mmol/L). Thus, positive and species-specific effects of seaweed on estimated uCP and fermentation parameters were observed in vitro when protein fractions remaining after an extraction procedure on seaweed partly replaced grass silage in the feed ration.
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Iodine content in bulk biomass of wild-harvested and cultivated edible seaweeds: Inherent variations determine species-specific daily allowable consumption
Food Chemistry, 2018Co-Authors: Michael Y. Roleda, Céline Rebours, Anna Gietl, Helene Marfaing, Dagmar B Stengel, Jorunn Skjermo, Rosa Jonsdottir, Udo NitschkeAbstract:Abstract This study represents a large-scale investigation into iodine contents in three commercially important and edible seaweed species from the North Atlantic: the brown algae Saccharina latissima and Alaria esculenta , and the red alga Palmaria palmata . Variability among and within species were explored in terms of temporal and spatial variations in addition to biomass source. Mean iodine concentration in bulk seaweed biomass was species-specific: Saccharina > Alaria > Palmaria . Iodine contents of Saccharina biomass were similar between years and seasons, but varied significantly between sampling locations and biomass sources. In Alaria and Palmaria , none of the independent variables examined contributed significantly to the small variations observed. Our data suggest that all three species are rich sources of iodine, and only 32, 283, or 2149 mg dry weight of unprocessed dry biomass of Saccharina , Alaria , or Palmaria , respectively, meets the recommended daily intake levels for most healthy humans.
Udo Nitschke - One of the best experts on this subject based on the ideXlab platform.
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Iodine content in bulk biomass of wild-harvested and cultivated edible seaweeds: Inherent variations determine species-specific daily allowable consumption
Food Chemistry, 2018Co-Authors: Michael Y. Roleda, Céline Rebours, Anna Gietl, Helene Marfaing, Dagmar B Stengel, Jorunn Skjermo, Rosa Jonsdottir, Udo NitschkeAbstract:Abstract This study represents a large-scale investigation into iodine contents in three commercially important and edible seaweed species from the North Atlantic: the brown algae Saccharina latissima and Alaria esculenta , and the red alga Palmaria palmata . Variability among and within species were explored in terms of temporal and spatial variations in addition to biomass source. Mean iodine concentration in bulk seaweed biomass was species-specific: Saccharina > Alaria > Palmaria . Iodine contents of Saccharina biomass were similar between years and seasons, but varied significantly between sampling locations and biomass sources. In Alaria and Palmaria , none of the independent variables examined contributed significantly to the small variations observed. Our data suggest that all three species are rich sources of iodine, and only 32, 283, or 2149 mg dry weight of unprocessed dry biomass of Saccharina , Alaria , or Palmaria , respectively, meets the recommended daily intake levels for most healthy humans.
Michael D Guiry - One of the best experts on this subject based on the ideXlab platform.
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are north atlantic Alaria esculenta and a grandifolia Alariaceae phaeophyceae conspecific
European Journal of Phycology, 2001Co-Authors: Stefan Kraan, Jan Rueness, Michael D GuiryAbstract:Alaria (Alariaceae, Phaeophyceae) is a common genus of kelps in the northern hemisphere. Fourteen species are currently recognized, of which three, Alaria esculenta (L.) Greville, A. pylaii (Bory de Saint-Vincent) Greville and A. grandifolia J. Agardh, are reported for the coldtemperate North Atlantic Ocean. Alaria esculenta, the type species described originally from the North Atlantic, exhibits a range of biogeographically correlated morphotypes suggesting the possibility of multiple species, subspecies or hybrids. In Ireland we discovered an A. esculenta population with unusually long stipes resembling the type specimen of A. grandifolia described from Spitsbergen by J. Agardh in 1872. These and other plants of A. esculenta from Ireland were compared with plants from Spitsbergen fitting the description of A. grandifolia, using sexual hybridization, relative growth rate measurements and DNA sequence comparisons. Complete interfertility was observed between the different isolates. Three nucleotide substi...
Håvard Steinshamn - One of the best experts on this subject based on the ideXlab platform.
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In vitro evaluation of utilisable crude protein and methane production for a diet in which grass silage was replaced by different levels and fractions of extracted seaweed proteins
Animal Feed Science and Technology, 2019Co-Authors: Maryam Ramin, Inga Marie Aasen, Michael Y. Roleda, Marcia De Oliveira Franco, Marten Hetta, Håvard SteinshamnAbstract:Abstract Utilisable crude protein (uCP), methane (CH 4 ) production and other fermentation parameters were analysed in vitro for a diet in which grass silage was replaced by different levels of seaweed protein fractions prepared from three seaweed species: Saccharina latissima , Alaria esculenta and Palmaria palmata . Ten fractions from these three species in which the protein content had been increased and the salt content reduced by simple processing were tested, with inclusion levels in the diet based on the nitrogen content of the fractions. Following an extraction procedure, four fractions from Saccharina latissima , three from Alaria esculenta and one from Palmaria palmata , were gradually included in the diet by replacing high quality silage with approximately 0, 0.15, 0.30 and 0.45 g/g DM, while two high-protein fractions of Palmaria palmata were tested at replacement levels of 0, 0.075, 0.15 and 0.225 g/g DM. To estimate fermentation parameters, 500 mg of each diet were incubated in bottles with 60 mL buffered rumen fluid. Estimated uCP increased linearly with increasing replacement rate of grass silage with seaweed protein fractions (from 158 g/kg DM to 206 g/kg DM on average for all fractions). Increasing protein fraction from the brown seaweed Saccharina latissima in the diet significantly increased true organic matter digestibility (OMD) (from on average 0.786 to 0.821). Organic matter digestibility decreased with increasing level of Alaria esculenta fractions (from on average 0.785 to 0.733), which also gave a linear decrease in CH 4 production (from on average 45.3 to 38.5 mL/g organic matter). As a result of decreased CH 4 production and OMD, total volatile fatty acid concentration decreased with increasing level of Alaria esculenta fractions (from on average 69.5 to 63.0 mmol/L). Thus, positive and species-specific effects of seaweed on estimated uCP and fermentation parameters were observed in vitro when protein fractions remaining after an extraction procedure on seaweed partly replaced grass silage in the feed ration.