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Ido Perlman – One of the best experts on this subject based on the ideXlab platform.

  • Infliximab exerts a dose-dependent effect on retinal safety in the Albino Rabbit
    Documenta Ophthalmologica, 2017
    Co-Authors: Shiri Zayit-soudry, Ido Perlman, Esther Zemel, Igor Vainer, Michael Mimouni, Melvin Rabena, Dante J. Pieramici, Anat Loewenstein
    Abstract:

    Purpose To assess the retinal toxicity of an intravitreal injection of infliximab, a monoclonal antiantibody to tumor necrnecrosis factor α, in a Rabbit model. Materials and methods Two groups of adult Albino Rabbits ( n  = 5) received intravitreal injections of infliximab (0.1 ml) in the study eye and balanced salt solution (BSS, 0.1 ml) in the control eye at baseline. Group 1 was administered with 1.5 mg/0.1 ml, and group 2 was injected with 7.5 mg/0.1 ml of infliximab solution. Electroretinography (ERG) was performed at baseline and at 1, 7, 30, and 45 days after the injection. Visual evoked potentials (VEPs) were recorded at 7 and 45 days after the injection. After the last electrophysiological assessment, the Rabbits were euthanized and retinal histopathology and immunhistochemistry for glial fibrillary acidic protein (GFAP) were performed. Results ERG responses demonstrated no significant deficit in retinal function in eyes injected with infliximab. Mean dark-adapted a-wave and b-wave maximal amplitude and semi-saturation constant values at baseline and throughout the 45 days of follow-up after the injection indicated no remarkable difference in outer retinal function between the control and experimental eyes. VEP responses were similar at each time point (7 and 45 days). No difference was seen in retinal histopathology and immunocytochemistry sections in eyes receiving the 1.5 mg/0.1 ml dose compared to the control eyes. However, increased GFAP labeling in retinal Müller cells was detected in Rabbit eyes treated with the 7.5 mg/0.1 ml dose. Conclusions Intravitreal injection of 1.5 mg/0.1 ml infliximab dose has no toxic effect on the integrity (functional or structural) of the retina in Rabbits. A higher dose of 7.5 mg/0.1 ml may be slightly toxic as suggested by positive Müller cell GFAP expression. Additional studies of retinal toxicity at higher doses and after multiple injections are needed to establish the retinal safety of intravitreal infliximab therapy in humans.

  • infliximab exerts a dose dependent effect on retinal safety in the Albino Rabbit
    Documenta Ophthalmologica, 2017
    Co-Authors: Shiri Zayitsoudry, Ido Perlman, Esther Zemel, Igor Vainer, Michael Mimouni, Melvin Rabena, Dante J. Pieramici
    Abstract:

    To assess the retinal toxicity of an intravitreal injection of infliximab, a monoclonal antiantibody to tumor necrnecrosis factor α, in a Rabbit model. Two groups of adult Albino Rabbits (n = 5) received intravitreal injections of infliximab (0.1 ml) in the study eye and balanced salt solution (BSS, 0.1 ml) in the control eye at baseline. Group 1 was administered with 1.5 mg/0.1 ml, and group 2 was injected with 7.5 mg/0.1 ml of infliximab solution. Electroretinography (ERG) was performed at baseline and at 1, 7, 30, and 45 days after the injection. Visual evoked potentials (VEPs) were recorded at 7 and 45 days after the injection. After the last electrophysiological assessment, the Rabbits were euthanized and retinal histopathology and immunhistochemistry for glial fibrillary acidic protein (GFAP) were performed. ERG responses demonstrated no significant deficit in retinal function in eyes injected with infliximab. Mean dark-adapted a-wave and b-wave maximal amplitude and semi-saturation constant values at baseline and throughout the 45 days of follow-up after the injection indicated no remarkable difference in outer retinal function between the control and experimental eyes. VEP responses were similar at each time point (7 and 45 days). No difference was seen in retinal histopathology and immunocytochemistry sections in eyes receiving the 1.5 mg/0.1 ml dose compared to the control eyes. However, increased GFAP labeling in retinal Muller cells was detected in Rabbit eyes treated with the 7.5 mg/0.1 ml dose. Intravitreal injection of 1.5 mg/0.1 ml infliximab dose has no toxic effect on the integrity (functional or structural) of the retina in Rabbits. A higher dose of 7.5 mg/0.1 ml may be slightly toxic as suggested by positive Muller cell GFAP expression. Additional studies of retinal toxicity at higher doses and after multiple injections are needed to establish the retinal safety of intravitreal infliximab therapy in humans.

  • Safety of intravitreal clindamycin in Albino Rabbit eyes.
    Documenta ophthalmologica. Advances in ophthalmology, 2017
    Co-Authors: Zohar Habot-wilner, Anat Loewenstein, Orit Mazza, Jonathan Shahar, Amir Massarweh, Irit Mann, Ido Perlman
    Abstract:

    To study the potential toxic effects of intravitreal clindamycin on the retina of Albino Rabbits, by assessing functional and morphological retinal changes. Eight Albino Rabbits were included in the study. In each Rabbit, 1 mg/0.1 ml clindamycin was injected into the vitreous of the right (experimental) eye, and 0.1 ml saline was injected into the vitreous of the left (control) eye. The electroretinogram (ERG) was recorded before injection, 3 days, 1, 2, and 4 weeks post-injection. The visual evoked potential (VEP) was recorded 4 weeks post-injection. Clinical examination was conducted at all time points. The eyes were enucleated at the termination of the follow-up period in order to prepare the retinas for histology in order to assess retinal structure. ERG and VEP responses that were recorded from the experimental eye at different times following intravitreal clindamycin injection were very similar to the corresponding responses that were recorded from the control eyes. Clinical examination was normal in all eyes, and no histological damage was observed. Intravitreal injection of 1 mg clindamycin does not cause functional or morphological signs of retinal toxicity in Albino Rabbits, during a period of 4 weeks post-injection. These findings support the clinical use of 1 mg intravitreal clindamycin.

Anat Loewenstein – One of the best experts on this subject based on the ideXlab platform.

  • Infliximab exerts a dose-dependent effect on retinal safety in the Albino Rabbit
    Documenta Ophthalmologica, 2017
    Co-Authors: Shiri Zayit-soudry, Ido Perlman, Esther Zemel, Igor Vainer, Michael Mimouni, Melvin Rabena, Dante J. Pieramici, Anat Loewenstein
    Abstract:

    Purpose To assess the retinal toxicity of an intravitreal injection of infliximab, a monoclonal antibody to tumor necrosis factor α, in a Rabbit model. Materials and methods Two groups of adult Albino Rabbits ( n  = 5) received intravitreal injections of infliximab (0.1 ml) in the study eye and balanced salt solution (BSS, 0.1 ml) in the control eye at baseline. Group 1 was administered with 1.5 mg/0.1 ml, and group 2 was injected with 7.5 mg/0.1 ml of infliximab solution. Electroretinography (ERG) was performed at baseline and at 1, 7, 30, and 45 days after the injection. Visual evoked potentials (VEPs) were recorded at 7 and 45 days after the injection. After the last electrophysiological assessment, the Rabbits were euthanized and retinal histopathology and immunhistochemistry for glial fibrillary acidic protein (GFAP) were performed. Results ERG responses demonstrated no significant deficit in retinal function in eyes injected with infliximab. Mean dark-adapted a-wave and b-wave maximal amplitude and semi-saturation constant values at baseline and throughout the 45 days of follow-up after the injection indicated no remarkable difference in outer retinal function between the control and experimental eyes. VEP responses were similar at each time point (7 and 45 days). No difference was seen in retinal histopathology and immunocytochemistry sections in eyes receiving the 1.5 mg/0.1 ml dose compared to the control eyes. However, increased GFAP labeling in retinal Müller cells was detected in Rabbit eyes treated with the 7.5 mg/0.1 ml dose. Conclusions Intravitreal injection of 1.5 mg/0.1 ml infliximab dose has no toxic effect on the integrity (functional or structural) of the retina in Rabbits. A higher dose of 7.5 mg/0.1 ml may be slightly toxic as suggested by positive Müller cell GFAP expression. Additional studies of retinal toxicity at higher doses and after multiple injections are needed to establish the retinal safety of intravitreal infliximab therapy in humans.

  • Safety of intravitreal clindamycin in Albino Rabbit eyes.
    Documenta ophthalmologica. Advances in ophthalmology, 2017
    Co-Authors: Zohar Habot-wilner, Anat Loewenstein, Orit Mazza, Jonathan Shahar, Amir Massarweh, Irit Mann, Ido Perlman
    Abstract:

    To study the potential toxic effects of intravitreal clindamycin on the retina of Albino Rabbits, by assessing functional and morphological retinal changes. Eight Albino Rabbits were included in the study. In each Rabbit, 1 mg/0.1 ml clindamycin was injected into the vitreous of the right (experimental) eye, and 0.1 ml saline was injected into the vitreous of the left (control) eye. The electroretinogram (ERG) was recorded before injection, 3 days, 1, 2, and 4 weeks post-injection. The visual evoked potential (VEP) was recorded 4 weeks post-injection. Clinical examination was conducted at all time points. The eyes were enucleated at the termination of the follow-up period in order to prepare the retinas for histology in order to assess retinal structure. ERG and VEP responses that were recorded from the experimental eye at different times following intravitreal clindamycin injection were very similar to the corresponding responses that were recorded from the control eyes. Clinical examination was normal in all eyes, and no histological damage was observed. Intravitreal injection of 1 mg clindamycin does not cause functional or morphological signs of retinal toxicity in Albino Rabbits, during a period of 4 weeks post-injection. These findings support the clinical use of 1 mg intravitreal clindamycin.

  • physiological and toxicological effects of cefuroxime on the Albino Rabbit retina
    Investigative Ophthalmology & Visual Science, 2012
    Co-Authors: Jonathan Shahar, Esther Zemel, Ido Perlman, Anat Loewenstein
    Abstract:

    PURPOSE Intracameral cefuroxime was found to lower the risk of endophthalmitis after cataract surgery. The purpose of this study was to evaluate the retinal toxicity of cefuroxime in a Rabbit model. METHODS Twenty-two Albino Rabbits were divided into two cefuroxime groups: low-dose (1mg/0.1 mL, n = 9) and high dose (10 mg/0.1 mL, n = 13). The right eye of each Rabbit was injected with 0.1 mL cefuroxime solution (experimental eye) and the left eye with 0.1 mL saline (control eye). Electroretinogram (ERG) responses were recorded at 3 hours, 4 days, and 1, 2, and 4 weeks after injection. After 4 weeks, the Rabbits were euthanized, the eyes were enucleated, and the retinas were prepared for histologic evaluation and GFAP immunostaining. RESULTS No functional (ERG) or histologic damage was found in Rabbits in the low-dose group. In the high-dose group, a significant decrease in the ERG amplitudes of the experimental eyes was seen 3 hours after injection, followed by partial recovery during 4 weeks of follow-up. Retinal histology of experimental eyes revealed marked damage. GFAP immunoreactivity in Muller cells was expressed in Rabbits belonging to both groups, although it was more extensive in the high-dose group. CONCLUSIONS ERG and histologic findings indicated that a dose of 1 mg cefuroxime, administered intravitreally, was not toxic to the Rabbit retina. A dose of 10 mg, injected intravitreally, induced transient physiological effects, and was toxic to the Rabbit retina, as was evident by the permanent reduction in the ERG responses and by the structural damage to the retina with signs of glial activation.

Vincent H.l. Lee – One of the best experts on this subject based on the ideXlab platform.

Esther Zemel – One of the best experts on this subject based on the ideXlab platform.

  • Infliximab exerts a dose-dependent effect on retinal safety in the Albino Rabbit
    Documenta Ophthalmologica, 2017
    Co-Authors: Shiri Zayit-soudry, Ido Perlman, Esther Zemel, Igor Vainer, Michael Mimouni, Melvin Rabena, Dante J. Pieramici, Anat Loewenstein
    Abstract:

    Purpose To assess the retinal toxicity of an intravitreal injection of infliximab, a monoclonal antibody to tumor necrosis factor α, in a Rabbit model. Materials and methods Two groups of adult Albino Rabbits ( n  = 5) received intravitreal injections of infliximab (0.1 ml) in the study eye and balanced salt solution (BSS, 0.1 ml) in the control eye at baseline. Group 1 was administered with 1.5 mg/0.1 ml, and group 2 was injected with 7.5 mg/0.1 ml of infliximab solution. Electroretinography (ERG) was performed at baseline and at 1, 7, 30, and 45 days after the injection. Visual evoked potentials (VEPs) were recorded at 7 and 45 days after the injection. After the last electrophysiological assessment, the Rabbits were euthanized and retinal histopathology and immunhistochemistry for glial fibrillary acidic protein (GFAP) were performed. Results ERG responses demonstrated no significant deficit in retinal function in eyes injected with infliximab. Mean dark-adapted a-wave and b-wave maximal amplitude and semi-saturation constant values at baseline and throughout the 45 days of follow-up after the injection indicated no remarkable difference in outer retinal function between the control and experimental eyes. VEP responses were similar at each time point (7 and 45 days). No difference was seen in retinal histopathology and immunocytochemistry sections in eyes receiving the 1.5 mg/0.1 ml dose compared to the control eyes. However, increased GFAP labeling in retinal Müller cells was detected in Rabbit eyes treated with the 7.5 mg/0.1 ml dose. Conclusions Intravitreal injection of 1.5 mg/0.1 ml infliximab dose has no toxic effect on the integrity (functional or structural) of the retina in Rabbits. A higher dose of 7.5 mg/0.1 ml may be slightly toxic as suggested by positive Müller cell GFAP expression. Additional studies of retinal toxicity at higher doses and after multiple injections are needed to establish the retinal safety of intravitreal infliximab therapy in humans.

  • infliximab exerts a dose dependent effect on retinal safety in the Albino Rabbit
    Documenta Ophthalmologica, 2017
    Co-Authors: Shiri Zayitsoudry, Ido Perlman, Esther Zemel, Igor Vainer, Michael Mimouni, Melvin Rabena, Dante J. Pieramici
    Abstract:

    To assess the retinal toxicity of an intravitreal injection of infliximab, a monoclonal antibody to tumor necrosis factor α, in a Rabbit model. Two groups of adult Albino Rabbits (n = 5) received intravitreal injections of infliximab (0.1 ml) in the study eye and balanced salt solution (BSS, 0.1 ml) in the control eye at baseline. Group 1 was administered with 1.5 mg/0.1 ml, and group 2 was injected with 7.5 mg/0.1 ml of infliximab solution. Electroretinography (ERG) was performed at baseline and at 1, 7, 30, and 45 days after the injection. Visual evoked potentials (VEPs) were recorded at 7 and 45 days after the injection. After the last electrophysiological assessment, the Rabbits were euthanized and retinal histopathology and immunhistochemistry for glial fibrillary acidic protein (GFAP) were performed. ERG responses demonstrated no significant deficit in retinal function in eyes injected with infliximab. Mean dark-adapted a-wave and b-wave maximal amplitude and semi-saturation constant values at baseline and throughout the 45 days of follow-up after the injection indicated no remarkable difference in outer retinal function between the control and experimental eyes. VEP responses were similar at each time point (7 and 45 days). No difference was seen in retinal histopathology and immunocytochemistry sections in eyes receiving the 1.5 mg/0.1 ml dose compared to the control eyes. However, increased GFAP labeling in retinal Muller cells was detected in Rabbit eyes treated with the 7.5 mg/0.1 ml dose. Intravitreal injection of 1.5 mg/0.1 ml infliximab dose has no toxic effect on the integrity (functional or structural) of the retina in Rabbits. A higher dose of 7.5 mg/0.1 ml may be slightly toxic as suggested by positive Muller cell GFAP expression. Additional studies of retinal toxicity at higher doses and after multiple injections are needed to establish the retinal safety of intravitreal infliximab therapy in humans.

  • physiological and toxicological effects of cefuroxime on the Albino Rabbit retina
    Investigative Ophthalmology & Visual Science, 2012
    Co-Authors: Jonathan Shahar, Esther Zemel, Ido Perlman, Anat Loewenstein
    Abstract:

    PURPOSE Intracameral cefuroxime was found to lower the risk of endophthalmitis after cataract surgery. The purpose of this study was to evaluate the retinal toxicity of cefuroxime in a Rabbit model. METHODS Twenty-two Albino Rabbits were divided into two cefuroxime groups: low-dose (1mg/0.1 mL, n = 9) and high dose (10 mg/0.1 mL, n = 13). The right eye of each Rabbit was injected with 0.1 mL cefuroxime solution (experimental eye) and the left eye with 0.1 mL saline (control eye). Electroretinogram (ERG) responses were recorded at 3 hours, 4 days, and 1, 2, and 4 weeks after injection. After 4 weeks, the Rabbits were euthanized, the eyes were enucleated, and the retinas were prepared for histologic evaluation and GFAP immunostaining. RESULTS No functional (ERG) or histologic damage was found in Rabbits in the low-dose group. In the high-dose group, a significant decrease in the ERG amplitudes of the experimental eyes was seen 3 hours after injection, followed by partial recovery during 4 weeks of follow-up. Retinal histology of experimental eyes revealed marked damage. GFAP immunoreactivity in Muller cells was expressed in Rabbits belonging to both groups, although it was more extensive in the high-dose group. CONCLUSIONS ERG and histologic findings indicated that a dose of 1 mg cefuroxime, administered intravitreally, was not toxic to the Rabbit retina. A dose of 10 mg, injected intravitreally, induced transient physiological effects, and was toxic to the Rabbit retina, as was evident by the permanent reduction in the ERG responses and by the structural damage to the retina with signs of glial activation.

Ruponen Marika – One of the best experts on this subject based on the ideXlab platform.