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Peter Vandamme - One of the best experts on this subject based on the ideXlab platform.
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kerstersia gyiorum gen nov sp nov a novel Alcaligenes faecalis like organism isolated from human clinical samples and reclassification of Alcaligenes denitrificans ruger and tan 1983 as achromobacter denitrificans comb nov
International Journal of Systematic and Evolutionary Microbiology, 2003Co-Authors: Tom Coenye, Enevold Falsen, Marc Vancanneyt, Margo Cnockaert, Jean Swings, Peter VandammeAbstract:A polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. Phenotypically, these isolates resembled Alcaligenes faecalis. Whole-cell protein analysis distinguished two different species, and this was confirmed by DNA–DNA hybridizations. Cellular fatty acid analysis and 16S rDNA sequence analysis indicated that these isolates were related to the genera Alcaligenes, Bordetella, Achromobacter and Pigmentiphaga and belonged to the family Alcaligenaceae. On the basis of the results of this study, the organisms were classified in a novel genus, Kerstersia gen. nov. This genus comprises one species, Kerstersia gyiorum sp. nov. (type strain LMG 5906T=API 184-2-84T=CCUG 47000T), and several unnamed isolates. The DNA G+C content of members of the genus Kerstersia is between 61·5 and 62·9 mol%. On the basis of previously published DNA–DNA hybridization results and data from chemotaxonomic studies, it is proposed that Alcaligenes denitrificans Ruger and Tan 1983 be reclassified as Achromobacter denitrificans comb. nov.
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bordetella trematum sp nov isolated from wounds and ear infections in humans and reassessment of Alcaligenes denitrificans ruger and tan 1983
International Journal of Systematic and Evolutionary Microbiology, 1996Co-Authors: Peter Vandamme, K. Kersters, Marc Heyndrickx, M Vancanneyt, Bart Hoste, Enevold Falsen, K H HinzAbstract:Ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family Alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. This species, for which we propose the name Bordetella trematum sp. nov., was more closely related to the type species of the genus Bordetella (Bordetella pertussis) than to the type species of the genus Alcaligenes (Alcaligenes faecalis) and had the general characteristics of members of this family (i.e., a DNA base ratio in the range from 57 to 70 mol%, a fatty acid profile characterized by high percentages of 16:0, 17:0 cyclo, and 14:0 30H, nonsaccharolytic metabolism, and several classical biochemical characteristics, including aerobic and microaerobic growth, catalase activity, assimilation of citrate, an absence of anaerobic growth, and an absence of acetylmethylcarbinol and indole production, gelatin liquefaction, and esculin hydrolysis). A reevaluation of the criteria used to classify Alcaligenes denitrificans Ruger and Tan 1983 and Achromobacter xylosoxidans Yabuuchi and Ohyama 1971 as subspecies of Alcaligenes xylosoxidans and additional evidence provided in recent studies revealed that, consistent with present standards, it is appropriate to consider these two taxa distinct species of the genus Alcaligenes.
K H Hinz - One of the best experts on this subject based on the ideXlab platform.
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bordetella trematum sp nov isolated from wounds and ear infections in humans and reassessment of Alcaligenes denitrificans ruger and tan 1983
International Journal of Systematic and Evolutionary Microbiology, 1996Co-Authors: Peter Vandamme, K. Kersters, Marc Heyndrickx, M Vancanneyt, Bart Hoste, Enevold Falsen, K H HinzAbstract:Ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family Alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. This species, for which we propose the name Bordetella trematum sp. nov., was more closely related to the type species of the genus Bordetella (Bordetella pertussis) than to the type species of the genus Alcaligenes (Alcaligenes faecalis) and had the general characteristics of members of this family (i.e., a DNA base ratio in the range from 57 to 70 mol%, a fatty acid profile characterized by high percentages of 16:0, 17:0 cyclo, and 14:0 30H, nonsaccharolytic metabolism, and several classical biochemical characteristics, including aerobic and microaerobic growth, catalase activity, assimilation of citrate, an absence of anaerobic growth, and an absence of acetylmethylcarbinol and indole production, gelatin liquefaction, and esculin hydrolysis). A reevaluation of the criteria used to classify Alcaligenes denitrificans Ruger and Tan 1983 and Achromobacter xylosoxidans Yabuuchi and Ohyama 1971 as subspecies of Alcaligenes xylosoxidans and additional evidence provided in recent studies revealed that, consistent with present standards, it is appropriate to consider these two taxa distinct species of the genus Alcaligenes.
Enevold Falsen - One of the best experts on this subject based on the ideXlab platform.
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kerstersia gyiorum gen nov sp nov a novel Alcaligenes faecalis like organism isolated from human clinical samples and reclassification of Alcaligenes denitrificans ruger and tan 1983 as achromobacter denitrificans comb nov
International Journal of Systematic and Evolutionary Microbiology, 2003Co-Authors: Tom Coenye, Enevold Falsen, Marc Vancanneyt, Margo Cnockaert, Jean Swings, Peter VandammeAbstract:A polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. Phenotypically, these isolates resembled Alcaligenes faecalis. Whole-cell protein analysis distinguished two different species, and this was confirmed by DNA–DNA hybridizations. Cellular fatty acid analysis and 16S rDNA sequence analysis indicated that these isolates were related to the genera Alcaligenes, Bordetella, Achromobacter and Pigmentiphaga and belonged to the family Alcaligenaceae. On the basis of the results of this study, the organisms were classified in a novel genus, Kerstersia gen. nov. This genus comprises one species, Kerstersia gyiorum sp. nov. (type strain LMG 5906T=API 184-2-84T=CCUG 47000T), and several unnamed isolates. The DNA G+C content of members of the genus Kerstersia is between 61·5 and 62·9 mol%. On the basis of previously published DNA–DNA hybridization results and data from chemotaxonomic studies, it is proposed that Alcaligenes denitrificans Ruger and Tan 1983 be reclassified as Achromobacter denitrificans comb. nov.
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bordetella trematum sp nov isolated from wounds and ear infections in humans and reassessment of Alcaligenes denitrificans ruger and tan 1983
International Journal of Systematic and Evolutionary Microbiology, 1996Co-Authors: Peter Vandamme, K. Kersters, Marc Heyndrickx, M Vancanneyt, Bart Hoste, Enevold Falsen, K H HinzAbstract:Ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family Alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. This species, for which we propose the name Bordetella trematum sp. nov., was more closely related to the type species of the genus Bordetella (Bordetella pertussis) than to the type species of the genus Alcaligenes (Alcaligenes faecalis) and had the general characteristics of members of this family (i.e., a DNA base ratio in the range from 57 to 70 mol%, a fatty acid profile characterized by high percentages of 16:0, 17:0 cyclo, and 14:0 30H, nonsaccharolytic metabolism, and several classical biochemical characteristics, including aerobic and microaerobic growth, catalase activity, assimilation of citrate, an absence of anaerobic growth, and an absence of acetylmethylcarbinol and indole production, gelatin liquefaction, and esculin hydrolysis). A reevaluation of the criteria used to classify Alcaligenes denitrificans Ruger and Tan 1983 and Achromobacter xylosoxidans Yabuuchi and Ohyama 1971 as subspecies of Alcaligenes xylosoxidans and additional evidence provided in recent studies revealed that, consistent with present standards, it is appropriate to consider these two taxa distinct species of the genus Alcaligenes.
Wenjun Li - One of the best experts on this subject based on the ideXlab platform.
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Alcaligenes endophyticus sp nov isolated from roots of ammodendron bifolium
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Chunyan Lu, Yuqian Li, Ye Tian, Yanru Li, Dengdi An, Wenjun LiAbstract:A Gram-stain-negative, rod-shaped, motile bacterium, designated AER10T, was isolated from the roots of Ammodendron bifolium collected from Takeermohuer desert in Xinjiang Uygur Autonomous Region, northwestern China. Growth was found to occur from 10 to 45 °C, at pH 5.0–9.0, and could tolerate up to 10 % (w/v) NaCl. 16S rRNA gene sequence result indicated that the strain AER10T belongs to the genus Alcaligenes and was closely related to Alcaligenes aquatilis (98.4 %), Alcaligenes faecalis subsp. parafaecalis (98.4 %), Alcaligenes faecalis subsp. faecalis (98.1 %) and Alcaligenes faecalis subsp. phenolicus (97.9 %). However, the DNA–DNA hybridization values between the strain AER10T and the above strains were less than the threshold value (below 70 %) for the delineation of genomic species. The DNA G+C content was 53.3 mol%. Ubiquinone-8 (Q-8) was the only quinone system present. The major fatty acids were summed feature 8 (C18 : 1ω7c, 25 %), C16 : 0 (24.2 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 19.3 %) and cyclo-C17 : 0 (10.5 %). The polar lipid profile of the strain AER10T consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, two unidentified aminolipids and five unknown polar lipids. On the basis of the evidence presented in this study, strain AER10T is a representative of a novel species in the genus Alcaligenes , for which the name Alcaligenes endophyticus sp. nov. is proposed. The type strain is AER10T (=DSM 100498T=KCTC 42688T).
Karl-paul Witzel - One of the best experts on this subject based on the ideXlab platform.
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Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples
Applied and Environmental Microbiology, 1998Co-Authors: Gesche Braker, Andreas Fesefeldt, Karl-paul WitzelAbstract:A system was developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with PCR. Primer sequences were found for the amplification of fragments from both nitrite reductase genes (nirK and nirS) after comparative sequence analysis. Whenever amplification was tried with these primers, the known nir type of denitrifying laboratory cultures could be confirmed. Likewise, the method allowed a determination of the nir type of five laboratory strains. The nirK gene could be amplified from Blastobacter denitrificans, Alcaligenes xylosoxidans, and Alcaligenes sp. (DSM 30128); the nirS gene was amplified from Alcaligenes eutrophus DSM 530 and from the denitrifying isolate IFAM 3698. For each of the two genes, at least one primer combination amplified successfully for all of the test strains. Specific amplification products were not obtained with nondenitrifying bacteria or with strains of the other nir type. The specificity of the amplified products was confirmed by subsequent sequencing. These results suggest the suitability of the method for the qualitative detection of denitrifying bacteria in environmental samples. This was shown by applying one generally amplifying primer combination for each nir gene developed in this study to total DNA preparations from aquatic habitats.
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Development of PCR Primer Systems for Amplification of Nitrite Reductase Genes (\textit{nirK} and \textit{nirS}) To Detect Denitrifying Bacteria in Environmental Samples
Applied and Environmental Microbiology, 1998Co-Authors: Gesche Braker, Andreas Fesefeldt, Karl-paul WitzelAbstract:A system was developed for the detection of denitrifying bacteria\nby the amplification of specific nitrite reductase gene fragments\nwith PCR. Primer sequences were found for the amplification of fragments\nfrom both nitrite reductase genes (nirK and nirS) after comparative\nsequence analysis. Whenever amplification was tried with these primers,\nthe known nir type of denitrifying laboratory cultures could be confirmed.\nLikewise, the method allowed a determination of the nir type of five\nlaboratory strains. The nirK gene could be amplified from Blastobacter\ndenitrificans, Alcaligenes xylosoxidans, and Alcaligenes sp. (DSM\n30128); the nirS gene was amplified from Alcaligenes eutrophus DSM\n530 and from the denitrifying isolate IFAM 3698. For each of the\ntwo genes, at least one primer combination amplified successfully\nfor all of the test strains. Specific amplification products were\nnot obtained with nondenitrifying bacteria or with strains of the\nother nir type. The specificity of the amplified products was confirmed\nby subsequent sequencing. These results suggest the suitability of\nthe method for the qualitative detection of denitrifying bacteria\nin environmental samples. This was shown by applying one generally\namplifying primer combination for each nir gene developed in this\nstudy to total DNA preparations from aquatic habitats.
