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Tamotsu Hoshino – One of the best experts on this subject based on the ideXlab platform.

  • draft genome sequence of cryophilic basidiomycetous yeast mrakia blollopis sk 4 isolated from an Algal Mat of naga ike lake in the skarvsnes ice free area east antarctica
    Genome Announcements, 2015
    Co-Authors: Masaharu Tsuji, Sakae Kudoh, Tamotsu Hoshino

    Abstract:

    Mrakia blollopis strain SK-4 was isolated from an Algal Mat of Naga-ike, a lake in Skarvsnes, East Antarctica. Here, we report the draft genome sequence of M. blollopis SK-4. This is the first report on the genome sequence of any cold-adapted fungal species.

  • an application of wastewater treatment in a cold environment and stable lipase production of antarctic basidiomycetous yeast mrakia blollopis
    PLOS ONE, 2013
    Co-Authors: Masaharu Tsuji, Tamotsu Hoshino, Sakae Kudoh, Yuji Yokota, Kodai Shimohara

    Abstract:

    Milk fat curdle in sewage is one of the refractory Materials for active sludge treatment under low temperature conditions. For the purpose of solving this problem by using a bio-remediation agent, we screened Antarctic yeasts and isolated SK-4 strain from Algal Mat of sediments of Naga-ike, a lake in Skarvsnes, East Antarctica. The yeast strain showed high nucleotide sequence homologies (>99.6%) to Mrakia blollopis CBS8921(T) in ITS and D1/D2 sequences and had two unique characteristics when applied on an active sludge; i.e., it showed a potential to use various carbon sources and to grow under vitamin-free conditions. Indeed, it showed a biochemical oxygen demand (BOD) removal rate that was 1.25-fold higher than that of the control. We considered that the improved BOD removal rate by applying SK-4 strain was based on its lipase activity and characteristics. Finally, we purified the lipase from SK-4 and found that the enzyme was quite stable under wide ranges of temperatures and pH, even in the presence of various metal ions and organic solvents. SK-4, therefore, is a promising bio-remediation agent for cleaning up unwanted milk fat curdles from dairy milk wastewater under low temperature conditions.

Assunta Giordano – One of the best experts on this subject based on the ideXlab platform.

  • oceanobacillus oncorhynchi subsp incaldanensis subsp nov an alkalitolerant halophile isolated from an Algal Mat collected from a sulfurous spring in campania italy and emended description of oceanobacillus oncorhynchi
    International Journal of Systematic and Evolutionary Microbiology, 2006
    Co-Authors: Ida Romano, Agata Gambacorta, Licia Lama, Barbara Nicolaus, Annarita Poli, Assunta Giordano

    Abstract:

    A halophilic, alkalitolerant bacterium, strain 20AGT, was isolated from an Algal Mat collected from a sulfurous spring located in Santa Maria Incaldana (Mondragone, Campania Region, southern Italy). The isolate is Gram-positive, ferments several carbohydrates and has motile, rod-shaped cells that do not sporulate. The isolate grows at pH 6·5–9·5 and in 5–20 % NaCl. On the basis of 16S rRNA gene sequence similarity, the strain was shown to belong to the genus Oceanobacillus; strain 20AGT showed 96·6 % 16S rRNA gene sequence similarity to the type strain of Oceanobacillus iheyensis, DSM 14371T, and 99·5 % similarity to Oceanobacillus oncorhynchi NCIMB 14022T. Levels of DNA–DNA relatedness between strain 20AGT and O. iheyensis DSM 14371T and O. oncorhynchi NCIMB 14022T were respectively 29·4 and 59·0 %. The G+C content of the DNA of strain 20AGT was 40·1 mol%. The predominant respiratory quinone was MK-7, phosphatidylglycerol and phosphatidylcholine were the predominant polar lipids and minor phospholipids were also detected. ai-C14 : 0, ai-C15 : 0 and i-C15 : 0 were the major fatty acids. Strain 20AGT accumulated osmolytes and produced exopolysaccharide. On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, isolate 20AGT should be designated as the type strain of a subspecies of Oceanobacillus oncorhynchi, for which the name Oceanobacillus oncorhynchi subsp incaldanensis subsp. nov. is proposed. The type strain is 20AGT (=DSM 16557T=ATCC BAA-954T).

  • Salinivibrio costicola subsp. alcaliphilus subsp. nov., a haloalkaliphilic aerobe from Campania Region (Italy).
    Systematic and applied microbiology, 2005
    Co-Authors: Ida Romano, Agata Gambacorta, Licia Lama, Barbara Nicolaus, Assunta Giordano

    Abstract:

    Abstract Phenotypic and phylogenetic studies were performed on unidentified Gram-negative staining, haloalkaliphilic aerobe and protease producer Salinivibrio -like organism recovered from a saltish spring with Algal Mat in the “Pozzo del Sale” site (Salt’s Well) in the Campania Region (South Italy). Phylogenetic analysis based on comparison of 16S rRNA gene sequences demonstrated that the isolate was related to species of Salinivibrio genus. The DNA–DNA hybridization of the type strain 18AG T with the most related Salinivibrio costicola subsp. costicola showed a re-association value of 72%. Based on the phenotypic distinctiveness of 18AG T strain and molecular, chemical and genetic evidence, it is proposed that strain 18AG T can be classified as S. costicola subsp. alcaliphilus , subsp. nov. The type strain of S. costicola subsp. alcaliphilus , is ATCC BAA-952 T ; DSM 16359 T .

  • planococcus rifietensis sp nov isolated from Algal Mat collected from a sulfurous spring in campania italy
    Systematic and Applied Microbiology, 2003
    Co-Authors: Ida Romano, Assunta Giordano, Licia Lama, Barbara Nicolaus, Agata Gambacorta

    Abstract:

    Summary The taxomony of strain M8, isolated from Algal Mat formed at the origin of a sulfurous spring in Rifieto (Savignano Irpino, Campania, Italy), was investigated in a polyphasic approach. The morphological, physiological and genetic characteristics were compared with of Planococcus and Planomicrobium species. The isolate grew optimally at pH 9.0, 1.8 M NaCl at 37 °C. The cells were Gram-positive cocci that form pairs, tetrads and aggregates of several cells. The isolate was aerobic/microaerophilic and accumulated glycine-betaine, as a major osmolyte, with minor components glutaMate and an unknown compound. M8 was able to hydrolyse X-Glc (5-bromo-4-chloro-3-indoyl β-d-glucopyranoside). The polar lipid profile consisted of phosphatidylglycerol and diphosphatidylglycerol as major components, and phosphocholine as a minor compound. MK8 was the only quinone found and the fatty acid composition was dominated by branched acids, mainly ai C15:0. The G+C content of DNA was 47.9% and its phylogenetic position was established by 16S rRNA gene sequencing as a member of the genus Planococcus. The DNA/DNA similarity of M8 to the type species Planococcus citreus was less than 55%. For this reason and for physiological and chemotaxonomic features, it is proposed to create a new species Planococcus rifietensis sp. nov.

Janelle R Thompson – One of the best experts on this subject based on the ideXlab platform.

  • insights from the draft genome of the subsection v stigoneMatales cyanobacterium hapalosiphon sp strain mrb220 associated with 2 mib production
    Standards in Genomic Sciences, 2016
    Co-Authors: Shu Harn Te, Janelle R Thompson

    Abstract:

    A non-axenic uniAlgal culture containing a Subsection V (StigoneMatales) cyanobacterium, Hapalosiphon strain MRB 220, was obtained from a benthic freshwater Algal Mat through multiple transfers following growth in sterile media. Physiological characterization demonstrated the culture was capable of nitrogen-fixation and production of the off flavor compound 2-methylisoborneol (2-MIB). Total DNA isolated from this culture was sequenced using Illumina HiSeq and de novo assembled into contigs. The genome of MRB 220 was separated from co-occurring heterotrophic bacteria using sequence homology and compositional approaches, and its purity was confirmed based on best BLAST hit classification and principle component analysis of the tetranucleotide frequencies of fragmented contigs. The genome of ~7.4 Mbp contains 6,345 protein coding genes with 4,320 of these having functional prediction including predicted pathways for biosynthesis of the secondary metabolite welwitindolinone. Analyses of 16S rRNA gene and whole genome sequence average nucleotide identity indicated close relatedness of MRB 220 to the genera Hapalosiphon and Fischerella within the order StigoneMatales. Microscopic examination showed that MRB 220 formed heterocystous branched filaments, thereby supporting identification of strain MRB 220 as a morphospecies of Hapalosiphon. Availability of the draft genome of Hapalosiphon strain MRB 220 enables future work to elucidate the pathway and dynamics for biosynthesis of 2-MIB and other secondary metabolites and understand the ecology and physiology of StigoneMatales cyanobacteria in tropical freshwaters.