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Allogeneic Tissue

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Frédéric Mallein-gerin – One of the best experts on this subject based on the ideXlab platform.

Yu Hin Man – One of the best experts on this subject based on the ideXlab platform.

  • epithelial mechanobiology skin wound healing and the stem cell niche
    Journal of The Mechanical Behavior of Biomedical Materials, 2013
    Co-Authors: Nicholas D Evans, Richard O C Oreffo, E Healy, Philipp J Thurner, Yu Hin Man

    Skin wound healing is a vital process that is important for re-establishing the epithelial barrier following disease or injury. Aberrant or delayed skin wound healing increases the risk of infection, causes patient morbidity, and may lead to the formation of scar Tissue. One of the most important events in wound healing is coverage of the wound with a new epithelial layer. This occurs when keratinocytes at the wound periphery divide and migrate to re-populate the wound bed. Many approaches are under investigation to promote and expedite this process, including the topical application of growth factors and the addition of autologous and Allogeneic Tissue or cell grafts. The mechanical environment of the wound site is also of fundamental importance for the rate and quality of wound healing. It is known that mechanical stress can influence wound healing by affecting the behaviour of cells within the dermis, but it remains unclear how mechanical forces affect the healing epidermis. Tensile forces are known to affect the behaviour of cells within epithelia, however, and the material properties of extracellular matrices, such as substrate stiffness, have been shown to affect the morphology, proliferation, differentiation and migration of many different cell types. In this review we will introduce the structure of the skin and the process of wound healing. We will then discuss the evidence for the effect of Tissue mechanics in re-epithelialisation and, in particular, on stem cell behaviour in the wound microenvironment and in intact skin. We will discuss how the elasticity, mechanical heterogeneity and topography of the wound extracellular matrix impact the rate and quality of wound healing, and how we may exploit this knowledge to expedite wound healing and mitigate scarring.

Anthony Ratcliffe – One of the best experts on this subject based on the ideXlab platform.

  • Repair of osteochondral defects with Allogeneic Tissue engineered cartilage implants.
    Clinical orthopaedics and related research, 1999
    Co-Authors: R. E. Schreiber, B. M. Ilten-kirby, N. S. Dunkelman, K. T. Symons, L. M. Rekettye, J. Willoughby, Anthony Ratcliffe

    The objective of this study was to evaluate the effect of Allogeneic Tissue engineered cartilage implants on healing of osteochondral defects. Rabbit chondrocytes were cultured in monolayer, then seeded onto biodegradable, three-dimensional polyglycolic acid meshes. Cartilage constructs were cultured hydrodynamically to yield Tissue with relatively more (mature) or less (immature) hyalinelike cartilage, as compared with adult rabbit articular cartcartilage. Osteochondral defects in the patellar grooves of both stifle joints either were left untreated or implanted with Allogeneic Tissue engineered cartilage. Histologic samples from in and around the defect sites were examined 3, 6, 9, and 12, and 24 months after surgery. By 9 months after surgery, defect sites treated with cartilage implants contained significantly greater amounts of hyalinelike cartilage with high levels of proteoglycan, and had a smooth, nonfibrillated articular surface as compared to untreated defects. In contrast, the repair Tissue formed in untreated defects had fibrillated articular surfaces, significant amounts of fibrocartilage, and negligible proteoglycan. These differences between treated and untreated defects persisted through 24 months after surgery. The results of this study suggest that the treatment of osteochondral lesions with Allogeneic Tissue engineered cartilage implants may lead to superior repair Tissue than that found in untreated osteochondral lesions.

Martin Trepel – One of the best experts on this subject based on the ideXlab platform.

Emeline Perrier-groult – One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of the biocompatibility and stability of Allogeneic Tissue-engineered cartilage in humanized mice
    PLoS ONE, 2019
    Co-Authors: Emeline Perrier-groult, Eléonore Pérès, Marielle Pasdeloup, Louis Gazzolo, Madeleine Duc Dodon, Frédéric Mallein-gerin

    Articular cartilage (AC) has poor capacities of regeneration and lesions often lead to osteoarthritis. Current AC reconstruction implies autologous chondrocyte implantation which requires Tissue sampling and grafting. An alternative approach would be to use scaffolds containing off-the-shelf Allogeneic human articular chondrocytes (HACs). To investigate tolerance of Allogeneic HACs by the human immune system, we developed a humanized mouse model implanted with Allogeneic cartilage constructs generated in vitro. A prerequisite of the study was to identify a scaffold that would not provoke inflammatory reaction in host. Therefore, we first compared the response of hu-mice to two biomaterials used in regenerative medicine, collagen sponge and agarose hydrogel. Four weeks after implantation in hu-mice, acellular collagen sponges, but not acellular agarose hydrogels, showed positive staining for CD3 (T lymphocytes) and CD68 (macrophages), suggesting that collagen scaffold elicits weak inflammatory reaction. These data led us to deepen our evaluation of the biocompatibility of Allogeneic Tissue-engineered cartilage by using agarose as scaffold. Agarose hydrogels were combined with Allogeneic HACs to reconstruct cartilage in vitro. Particular attention was paid to HLA-A2 compatibility between HACs to be grafted and immune human cells of hu-mice: HLA-A2+ or HLA-A2- HACs agarose hydrogels were cultured in the presence of a chondrogenic cocktail and implanted in HLA-A2+ hu-mice. After four weeks implantation and regardless of the HLA-A2 phenotype, chondrocytes were well-differentiated and produced cartilage matrix in agarose. In addition, no sign of T-cell or macrophage infiltration was seen in the cartilaginous constructs and no significant increase in subpopulations of T lymphocytes and monocytes was detected in peripheral blood and spleen. We show for the first time that humanized mouse represents a useful model to investigate human immune responsiveness to Tissue-engineered cartilage and our data together indicate that Allogeneic cartilage constructs can be suitable for cartilage engineering.