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Yannis D. Clonis - One of the best experts on this subject based on the ideXlab platform.
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isoenzyme and Allozyme specific inhibitors 2 2 dihydroxybenzophenones and their carbonyl n analogues that discriminate between human glutathione transferase a1 1 and p1 1 Allozymes
Chemical Biology & Drug Design, 2015Co-Authors: Foteini M Pouliou, Trias Thireou, Elias Eliopoulos, Petros G. Tsoungas, Nikolaos E. Labrou, Yannis D. ClonisAbstract:The selectivity of certain benzophenones and their carbonyl N-analogues was investigated towards the human GSTP1-1 Allozymes A, B and C involved in MDR. The Allozymes were purified from extracts derived from E. coli harbouring the plasmids pEXP5-CT/TOPO-TA-hGSTP1*A, pOXO4-hGSTP1*B or pOXO4-hGSTP1*C. Compound screening with each Allozyme activity indicated three compounds with appreciable inhibitory potencies, 12 and 13 with P1-1A 62% and 67%, 11 and 12 with P1-1C 51% and 70%, whereas that of 15 fell behind with P1-1B (41%). These findings were confirmed by IC50 values (74-125 μm). Enzyme inhibition kinetics, aided by molecular modelling and docking, revealed that there is competition with the substrate CDNB for the same binding site on the Allozyme (Ki(13/A) = 63.6 ± 3.0 μm, Ki(15/B) = 198.6 ± 14.3 μm, and Ki(11/C) = 16.5 ± 2.7 μm). These data were brought into context by an in silico structural comparative analysis of the targeted proteins. Although the screened compounds showed moderate inhibitory potency against hGSTP1-1, remarkably, some of them demonstrated absolute isoenzyme and/or Allozyme selectivity.
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Isoenzyme‐ and Allozyme‐Specific Inhibitors: 2,2′‐Dihydroxybenzophenones and Their Carbonyl N‐Analogues that Discriminate between Human Glutathione Transferase A1‐1 and P1‐1 Allozymes
Chemical biology & drug design, 2015Co-Authors: Foteini M Pouliou, Trias Thireou, Elias Eliopoulos, Petros G. Tsoungas, Nikolaos E. Labrou, Yannis D. ClonisAbstract:The selectivity of certain benzophenones and their carbonyl N-analogues was investigated towards the human GSTP1-1 Allozymes A, B and C involved in MDR. The Allozymes were purified from extracts derived from E. coli harbouring the plasmids pEXP5-CT/TOPO-TA-hGSTP1*A, pOXO4-hGSTP1*B or pOXO4-hGSTP1*C. Compound screening with each Allozyme activity indicated three compounds with appreciable inhibitory potencies, 12 and 13 with P1-1A 62% and 67%, 11 and 12 with P1-1C 51% and 70%, whereas that of 15 fell behind with P1-1B (41%). These findings were confirmed by IC50 values (74-125 μm). Enzyme inhibition kinetics, aided by molecular modelling and docking, revealed that there is competition with the substrate CDNB for the same binding site on the Allozyme (Ki(13/A) = 63.6 ± 3.0 μm, Ki(15/B) = 198.6 ± 14.3 μm, and Ki(11/C) = 16.5 ± 2.7 μm). These data were brought into context by an in silico structural comparative analysis of the targeted proteins. Although the screened compounds showed moderate inhibitory potency against hGSTP1-1, remarkably, some of them demonstrated absolute isoenzyme and/or Allozyme selectivity.
Patrick Berrebi - One of the best experts on this subject based on the ideXlab platform.
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Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighbouring Adriatic drainages as revealed by Allozyme and mitochondrial sequence data
Biological Journal of the Linnean Society, 2002Co-Authors: Costas S Tsigenopoulos, Petr Kotlik, Patrick BerrebiAbstract:Genetic relationships among 24 Italian and Adriatic populations of barbs (genus Barbus) were assessed using electrophoretic analysis of Allozymes and mitochondrial DNA sequences of the cytochrome b gene. Results obtained with both markers were concordant, but they were not congruent with the current morphology-based systematics and taxonomy. Populations assigned to the same nominal taxa (B. caninus, B. petenyi and B. rebeli), were very divergent in both Allozymes and mtDNA, indicating that these populations deserve recognition as different units for conservation and management. On the other hand, the two fluvio-lacustrine taxa considered as distinct species (i.e. B. plebejus and B. tyberinus) are genetically very close to each other, showing no clear differences at either Allozymes or mtDNA. The population of B. caninus from Pellice River carried Allozyme alleles and mtDNA specific for B. plebejus, indicating a genetic introgression towards the former species.
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biogeography and pattern of gene flow among barbus species teleostei cyprinidae inhabiting the italian peninsula and neighbouring adriatic drainages as revealed by Allozyme and mitochondrial sequence data
Biological Journal of The Linnean Society, 2002Co-Authors: Costas S Tsigenopoulos, Petr Kotlik, Patrick BerrebiAbstract:Genetic relationships among 24 Italian and Adriatic populations of barbs (genus Barbus) were assessed using electrophoretic analysis of Allozymes and mitochondrial DNA sequences of the cytochrome b gene. Results obtained with both markers were concordant, but they were not congruent with the current morphology-based systematics and taxonomy. Populations assigned to the same nominal taxa (B. caninus, B. petenyi and B. rebeli), were very divergent in both Allozymes and mtDNA, indicating that these populations deserve recognition as different units for conservation and management. On the other hand, the two fluvio-lacustrine taxa considered as distinct species (i.e. B. plebejus and B. tyberinus) are genetically very close to each other, showing no clear differences at either Allozymes or mtDNA. The population of B. caninus from Pellice River carried Allozyme alleles and mtDNA specific for B. plebejus, indicating a genetic introgression towards the former species. © 2002 The Linnean Society of London, Biological Journal of the Linnean Society, 2002, 75, 83–99.
Laura A. Katz - One of the best experts on this subject based on the ideXlab platform.
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Systematics of dusky salamanders, Desmognathus (Caudata: Plethodontidae), in the mountain and Piedmont regions of Virginia and North Carolina, USA
Zoological Journal of the Linnean Society, 2008Co-Authors: Stephen G. Tilley, Renée L. Eriksen, Laura A. KatzAbstract:We analysed mitochondrial (cytochrome b) nucleotide sequences, nuclear Allozyme markers, and morphometric characters to investigate species boundaries and phylogenetic relationships among dusky salamanders (Desmognathus) in the southern Blue Ridge and adjacent Piedmont Physiographic Provinces of Virginia and North Carolina. Our results revealed four distinct mitochondrial DNA clades that are also characterized by distinct Allozyme markers. One clade consists of sequences derived from populations distributed from New England to south-western Virginia that are referable to Desmognathus fuscus Rafinesque, 1820, although there is considerable sequence and Allozyme divergence within this clade. A second clade consists of sequences derived from populations referable to Desmognathus planiceps Newman, 1955, a form that we resurrect from its long synonymy under D. fuscus. Desmognathus planiceps and D. fuscus also differ in mandibular tooth shape. Two other cytochrome b sequences recovered from populations along the Blue Ridge escarpment in southern Virginia are quite divergent from those of the previous two clades, and these populations may represent yet another distinct species. Sequences from a population in the Brushy Mountains in the Piedmont of northern North Carolina are similar to those of Desmognathus carolinensis. Population groupings indicated by Allozyme data generally correspond to the cytochrome b clades. Cryptic diversity in Appalachian desmognathan salamanders clearly requires further study. © 2008 The Linnean Society of London, Zoological Journal of the Linnean Society, 2008, 152, 115‐130. ADDITIONAL KEYWORDS: Allozymes ‐ cytochrome b ‐ Desmognathus planiceps ‐ mitochondrial DNA ‐ morphometrics ‐ phylogeography ‐ taxonomy.
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genetic structure of the blue ridge dusky salamander desmognathus orestes inferences from Allozymes mitochondrial dna and behavior
Evolution, 2001Co-Authors: Louise S Mead, Stephen G. Tilley, Laura A. KatzAbstract:Abstract The plethodontid salamander Desmognathus orestes, a member of the D. ochrophaeus species complex, is distributed in southwestern Virginia, eastern Tennessee, and western North Carolina. Previous Allozyme analyses indicate that D. orestes consists of two distinct groups of populations (D. orestes ‘B’ and D. orestes ‘C’) with extensive intergradation and probable gene flow between these two groups. Spatially varying allele frequencies can reflect historical associations, current gene flow, or a combination of population-level processes. To differentiate among these processes, we use multiple markers to further characterize divergence among populations of D. orestes and assess the degree of intergradation between D. orestes ‘B’ and D. orestes ‘C’, specifically investigating variation in Allozymes, mitochondrial DNA (mtDNA), and reproductive behavior among populations. On a broad scale, the mtDNA genealogies reconstruct haplotype clades that correspond to the species identified from previous Allozyme...
Foteini M Pouliou - One of the best experts on this subject based on the ideXlab platform.
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isoenzyme and Allozyme specific inhibitors 2 2 dihydroxybenzophenones and their carbonyl n analogues that discriminate between human glutathione transferase a1 1 and p1 1 Allozymes
Chemical Biology & Drug Design, 2015Co-Authors: Foteini M Pouliou, Trias Thireou, Elias Eliopoulos, Petros G. Tsoungas, Nikolaos E. Labrou, Yannis D. ClonisAbstract:The selectivity of certain benzophenones and their carbonyl N-analogues was investigated towards the human GSTP1-1 Allozymes A, B and C involved in MDR. The Allozymes were purified from extracts derived from E. coli harbouring the plasmids pEXP5-CT/TOPO-TA-hGSTP1*A, pOXO4-hGSTP1*B or pOXO4-hGSTP1*C. Compound screening with each Allozyme activity indicated three compounds with appreciable inhibitory potencies, 12 and 13 with P1-1A 62% and 67%, 11 and 12 with P1-1C 51% and 70%, whereas that of 15 fell behind with P1-1B (41%). These findings were confirmed by IC50 values (74-125 μm). Enzyme inhibition kinetics, aided by molecular modelling and docking, revealed that there is competition with the substrate CDNB for the same binding site on the Allozyme (Ki(13/A) = 63.6 ± 3.0 μm, Ki(15/B) = 198.6 ± 14.3 μm, and Ki(11/C) = 16.5 ± 2.7 μm). These data were brought into context by an in silico structural comparative analysis of the targeted proteins. Although the screened compounds showed moderate inhibitory potency against hGSTP1-1, remarkably, some of them demonstrated absolute isoenzyme and/or Allozyme selectivity.
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Isoenzyme‐ and Allozyme‐Specific Inhibitors: 2,2′‐Dihydroxybenzophenones and Their Carbonyl N‐Analogues that Discriminate between Human Glutathione Transferase A1‐1 and P1‐1 Allozymes
Chemical biology & drug design, 2015Co-Authors: Foteini M Pouliou, Trias Thireou, Elias Eliopoulos, Petros G. Tsoungas, Nikolaos E. Labrou, Yannis D. ClonisAbstract:The selectivity of certain benzophenones and their carbonyl N-analogues was investigated towards the human GSTP1-1 Allozymes A, B and C involved in MDR. The Allozymes were purified from extracts derived from E. coli harbouring the plasmids pEXP5-CT/TOPO-TA-hGSTP1*A, pOXO4-hGSTP1*B or pOXO4-hGSTP1*C. Compound screening with each Allozyme activity indicated three compounds with appreciable inhibitory potencies, 12 and 13 with P1-1A 62% and 67%, 11 and 12 with P1-1C 51% and 70%, whereas that of 15 fell behind with P1-1B (41%). These findings were confirmed by IC50 values (74-125 μm). Enzyme inhibition kinetics, aided by molecular modelling and docking, revealed that there is competition with the substrate CDNB for the same binding site on the Allozyme (Ki(13/A) = 63.6 ± 3.0 μm, Ki(15/B) = 198.6 ± 14.3 μm, and Ki(11/C) = 16.5 ± 2.7 μm). These data were brought into context by an in silico structural comparative analysis of the targeted proteins. Although the screened compounds showed moderate inhibitory potency against hGSTP1-1, remarkably, some of them demonstrated absolute isoenzyme and/or Allozyme selectivity.
Costas S Tsigenopoulos - One of the best experts on this subject based on the ideXlab platform.
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Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighbouring Adriatic drainages as revealed by Allozyme and mitochondrial sequence data
Biological Journal of the Linnean Society, 2002Co-Authors: Costas S Tsigenopoulos, Petr Kotlik, Patrick BerrebiAbstract:Genetic relationships among 24 Italian and Adriatic populations of barbs (genus Barbus) were assessed using electrophoretic analysis of Allozymes and mitochondrial DNA sequences of the cytochrome b gene. Results obtained with both markers were concordant, but they were not congruent with the current morphology-based systematics and taxonomy. Populations assigned to the same nominal taxa (B. caninus, B. petenyi and B. rebeli), were very divergent in both Allozymes and mtDNA, indicating that these populations deserve recognition as different units for conservation and management. On the other hand, the two fluvio-lacustrine taxa considered as distinct species (i.e. B. plebejus and B. tyberinus) are genetically very close to each other, showing no clear differences at either Allozymes or mtDNA. The population of B. caninus from Pellice River carried Allozyme alleles and mtDNA specific for B. plebejus, indicating a genetic introgression towards the former species.
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biogeography and pattern of gene flow among barbus species teleostei cyprinidae inhabiting the italian peninsula and neighbouring adriatic drainages as revealed by Allozyme and mitochondrial sequence data
Biological Journal of The Linnean Society, 2002Co-Authors: Costas S Tsigenopoulos, Petr Kotlik, Patrick BerrebiAbstract:Genetic relationships among 24 Italian and Adriatic populations of barbs (genus Barbus) were assessed using electrophoretic analysis of Allozymes and mitochondrial DNA sequences of the cytochrome b gene. Results obtained with both markers were concordant, but they were not congruent with the current morphology-based systematics and taxonomy. Populations assigned to the same nominal taxa (B. caninus, B. petenyi and B. rebeli), were very divergent in both Allozymes and mtDNA, indicating that these populations deserve recognition as different units for conservation and management. On the other hand, the two fluvio-lacustrine taxa considered as distinct species (i.e. B. plebejus and B. tyberinus) are genetically very close to each other, showing no clear differences at either Allozymes or mtDNA. The population of B. caninus from Pellice River carried Allozyme alleles and mtDNA specific for B. plebejus, indicating a genetic introgression towards the former species. © 2002 The Linnean Society of London, Biological Journal of the Linnean Society, 2002, 75, 83–99.