The Experts below are selected from a list of 1038 Experts worldwide ranked by ideXlab platform
Ming Shu Wang - One of the best experts on this subject based on the ideXlab platform.
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DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication.
Veterinary microbiology, 2021Co-Authors: Ming Shu Wang, An Chun Cheng, Renyong Jia, Mafeng Liu, Qiao Yang, Juan Huang, Bin Tian, Shun ChenAbstract:The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread.
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Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1
Veterinary Research, 2020Co-Authors: Ming Shu Wang, An Chun Cheng, Shun Chen, Renyong Jia, Dekang Zhu, Mafeng Liu, Qiao Yang, Juan Huang, Xin-xin Zhao, Shaqiu ZhangAbstract:AbstractDuck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1).
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Bioinformatics Analysis and Characteristics of Us2 protein Encoded by the Newly Identified US2 Gene of Duck Enteritis Virus
2016Co-Authors: Jie Gao, An Chun Cheng, Ming Shu WangAbstract:Abstract. Us2 protein encoded by the DEV-US2 gene is one of the DEV tegument proteins. Like most members of the Alphaherpesvirinae, the genes in the US region of its genome are well conserved, which means that both the major DEV protein Us2 and Alphaherpesvirinae protein Us2 possess the similar functions. Us2 protein sequence analysis indicates that the protein possesses typical characteristics of tegument protein. The precursor Us2 consists of 239 amino acids and exhibits a molecular mass of 34 kDa. In conclusion, all the datas and consequences will provide a basis for further functional study of the DEV-Us2 protein and provide necessary datas for the new type clinical diagnosis of DEV and the development of new DEV vaccine
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Identification of the Nuclear Localization Signal Region of Duck Enteritis Virus UL14 and Its Interaction with VP16.
Intervirology, 2016Co-Authors: Yuwei Zhang, Ming Shu Wang, Shun Chen, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao YangAbstract:Object: Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae viruses. VP16 and pUL14 are both predicted to be tegument proteins of DEV.
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Bioinformatics Analysis of the Duck Enteritis Virus UL54 Gene
Research Journal of Applied Sciences Engineering and Technology, 2014Co-Authors: Chaoyue Liu, An Chun Cheng, Ming Shu WangAbstract:In this study, we analyze the Duck Enteritis Virus (DEV) UL54 gene, which has been isolated and identified in our lab (GenBank accession NO EU071033), to help deeply research on DEV. DNA sequence analysis showed that the identified ORF which composed of 1377 bp nucleotides encoded 458 amino acids with a predicted Mr. of 51.75 kDa. Multiple sequence alignment suggested that the UL54 gene was highly conserved in Alphaherpesvirinae and was similar to the other herpesviral UL54 gene. Phylogenetic analysis of the DEV UL54 gene revealed that DEV had a close evolutionary relationship with Gallid, Herpesvirus 2 (GaHV-2), Gallid Herpesvirus 3 (GaHV-3), Meleagrid Herpesvirus1 (MeHV-1) and should belong to a single cluster within the Alphaherpesvirinae subfamily.
An Chun Cheng - One of the best experts on this subject based on the ideXlab platform.
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DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication.
Veterinary microbiology, 2021Co-Authors: Ming Shu Wang, An Chun Cheng, Renyong Jia, Mafeng Liu, Qiao Yang, Juan Huang, Bin Tian, Shun ChenAbstract:The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread.
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Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1
Veterinary Research, 2020Co-Authors: Ming Shu Wang, An Chun Cheng, Shun Chen, Renyong Jia, Dekang Zhu, Mafeng Liu, Qiao Yang, Juan Huang, Xin-xin Zhao, Shaqiu ZhangAbstract:AbstractDuck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1).
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Bioinformatics Analysis and Characteristics of Us2 protein Encoded by the Newly Identified US2 Gene of Duck Enteritis Virus
2016Co-Authors: Jie Gao, An Chun Cheng, Ming Shu WangAbstract:Abstract. Us2 protein encoded by the DEV-US2 gene is one of the DEV tegument proteins. Like most members of the Alphaherpesvirinae, the genes in the US region of its genome are well conserved, which means that both the major DEV protein Us2 and Alphaherpesvirinae protein Us2 possess the similar functions. Us2 protein sequence analysis indicates that the protein possesses typical characteristics of tegument protein. The precursor Us2 consists of 239 amino acids and exhibits a molecular mass of 34 kDa. In conclusion, all the datas and consequences will provide a basis for further functional study of the DEV-Us2 protein and provide necessary datas for the new type clinical diagnosis of DEV and the development of new DEV vaccine
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Bioinformatics Analysis of the Duck Enteritis Virus UL54 Gene
Research Journal of Applied Sciences Engineering and Technology, 2014Co-Authors: Chaoyue Liu, An Chun Cheng, Ming Shu WangAbstract:In this study, we analyze the Duck Enteritis Virus (DEV) UL54 gene, which has been isolated and identified in our lab (GenBank accession NO EU071033), to help deeply research on DEV. DNA sequence analysis showed that the identified ORF which composed of 1377 bp nucleotides encoded 458 amino acids with a predicted Mr. of 51.75 kDa. Multiple sequence alignment suggested that the UL54 gene was highly conserved in Alphaherpesvirinae and was similar to the other herpesviral UL54 gene. Phylogenetic analysis of the DEV UL54 gene revealed that DEV had a close evolutionary relationship with Gallid, Herpesvirus 2 (GaHV-2), Gallid Herpesvirus 3 (GaHV-3), Meleagrid Herpesvirus1 (MeHV-1) and should belong to a single cluster within the Alphaherpesvirinae subfamily.
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BMEI - Characterization of codon usage bias in the US10 gene of duck enteritis virus
2012 5th International Conference on BioMedical Engineering and Informatics, 2012Co-Authors: An Chun Cheng, Ming Shu WangAbstract:In order to have some bioinformatics information about synonymous codon usage pattern of the duck enteritis virus (DEV) US10 gene (GenBank accession number: EU195084), we analyzed the DEV US10 gene and 19 other reference herpesviruses by using bioinformatics softwares, such as CAI, CHIPS and CUSP program of EMBOSS. The results revealed that codon usage bias in the US10 gene was mainly influenced by nucleotide composition, specifically manifested in adenine (A) and thymine (T) at the third codon position. The phylogenetic analysis suggested that DEV had a closer evolutionary relationship with the Mardivirus, which was clustered into Alphaherpesvirinae. There were 32 codons showing uniform usage between DEV and Escherichia coli, 41 between DEV and yeast, 38 between DEV and Homo sapiens. Therefore, the yeast expression system may be more appropriate for the expression of DEV US10 gene. The information from this research may not only have theoretical value in understanding molecular evolution, but also have potential value for the further studying of this gene.
D J Jeffries - One of the best experts on this subject based on the ideXlab platform.
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Molecular biology of varicella-zoster virus. A review prepared for the UK Advisory Group on Chickenpox.
The Journal of infection, 1998Co-Authors: D R Harper, R L Gilbert, D J JeffriesAbstract:Varicella-zoster virus (human herpesvirus 3; VZV) is one of eight herpes viruses that routinely infect humans. It is classified as a member of the genus Varicellovirus, subfamily Alphaherpesvirinae, family Herpesviridae. Of the other human herpes viruses it is most closely related to the herpes simplex viruses (also members of the Alphalerpesvirinae). Like all herpes viruses, the virus has a large double-stranded DNA genome within an icosahedral nucleocapsid. This is surrounded by a proteinaceous tegument and a trilaminar membrane derived from host-cell membranes into which the viral glycoproteins are inserted. The structure of the virion is summarized in Fig. 1.
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Molecular Biology of Varicella-zoster Virus: A review prepared for the UK Advisory Group on Chickenpox
Journal of Infection, 1998Co-Authors: D R Harper, R L Gilbert, D J JeffriesAbstract:Varicella-zoster virus (human herpesvirus 3; VZVI is one of eight viruses that routinely infect humans. It is classified as a member of the genus Varicellovirus , subfamily Alphaherpesvirinae , family Herpesviridae . Of the other human herpes viruses it is ost closely related to the herpes simplex viruses (also members of the Alphaherpesvirinae ). Like all herpes viruses, the virus has a large double-stranded DNA genome within an icosahedral nucleocapsid. This is surrounded by a proteinaceous tegument and a trilaminar memhbrane derived from host-cell membranes into which the viral glycoproteins are inserted. The structure of the virion is summarized in Fig. 1.
K. W. Wilcox - One of the best experts on this subject based on the ideXlab platform.
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The conserved DNA-binding domains encoded by the herpes simplex virus type 1 ICP4, pseudorabies virus IE180, and varicella-zoster virus ORF62 genes recognize similar sites in the corresponding promoters.
Journal of virology, 1991Co-Authors: K. W. WilcoxAbstract:Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), pseudorabies virus (PRV), varicella-zoster virus (VZV), and equine herpesvirus 1 (EHV-1) are all classified as Alphaherpesvirinae. Each of these five viruses encodes an essential immediate-early (IE) regulatory protein referred to as HSV-1 ICP4, HSV-2 ICP4, PRV IE180, VZV ORF62 protein, and EHV-1 IE1, respectively. These five proteins share extensive homology with each other in domains referred to as regions 2 and 4. The HSV-1 ICP4 region 2 domain contains residues that are required for the DNA-binding capability of ICP4. In this report, we describe the expression of region 2 domains from the ICP4, IE180, and ORF62 genes as fusion proteins in Escherichia coli. DNA-binding assays revealed that each of these region 2 fusion proteins binds to a sequence that overlaps the transcription start site in the promoter for the gene encoding the corresponding protein. Each of the sites with high affinity for one or more of these fusion proteins contains the sequence 5'-ATCGT-3'. This sequence spans the mRNA cap site in the HSV-2 ICP4 gene promoter and is immediately upstream from the transcription start site in the EHV-1 IE1 gene. These results suggest that formation of a specific complex between an IE protein and its own gene promoter may be a common mechanism used by Alphaherpesvirinae to autoregulate transcription of an essential IE gene.
D R Harper - One of the best experts on this subject based on the ideXlab platform.
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Molecular biology of varicella-zoster virus. A review prepared for the UK Advisory Group on Chickenpox.
The Journal of infection, 1998Co-Authors: D R Harper, R L Gilbert, D J JeffriesAbstract:Varicella-zoster virus (human herpesvirus 3; VZV) is one of eight herpes viruses that routinely infect humans. It is classified as a member of the genus Varicellovirus, subfamily Alphaherpesvirinae, family Herpesviridae. Of the other human herpes viruses it is most closely related to the herpes simplex viruses (also members of the Alphalerpesvirinae). Like all herpes viruses, the virus has a large double-stranded DNA genome within an icosahedral nucleocapsid. This is surrounded by a proteinaceous tegument and a trilaminar membrane derived from host-cell membranes into which the viral glycoproteins are inserted. The structure of the virion is summarized in Fig. 1.
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Molecular Biology of Varicella-zoster Virus: A review prepared for the UK Advisory Group on Chickenpox
Journal of Infection, 1998Co-Authors: D R Harper, R L Gilbert, D J JeffriesAbstract:Varicella-zoster virus (human herpesvirus 3; VZVI is one of eight viruses that routinely infect humans. It is classified as a member of the genus Varicellovirus , subfamily Alphaherpesvirinae , family Herpesviridae . Of the other human herpes viruses it is ost closely related to the herpes simplex viruses (also members of the Alphaherpesvirinae ). Like all herpes viruses, the virus has a large double-stranded DNA genome within an icosahedral nucleocapsid. This is surrounded by a proteinaceous tegument and a trilaminar memhbrane derived from host-cell membranes into which the viral glycoproteins are inserted. The structure of the virion is summarized in Fig. 1.