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Zhu L Yang - One of the best experts on this subject based on the ideXlab platform.
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the msdin family in amanitin producing mushrooms and evolution of the prolyl oligopeptidase genes
IMA fungus, 2018Co-Authors: Hong Luo, Qing Cai, Yungjiao Luli, Rohita Sinha, Heather E Hallenadams, Zhu L YangAbstract:The biosynthetic pathway for amanitins and related cyclic peptides in deadly Amanita (Amanitaceae) mushrooms represents the first known ribosomal cyclic peptide pathway in the Fungi. Amanitins are found outside of the genus in distantly related agarics Galerina (Strophariaceae) and Lepiota (Agaricaceae). A long-standing question in the field persists: why is this pathway present in these phylogenetically disjunct agarics? Two deadly mushrooms, A. pallidorosea and A. subjunquillea, were deep sequenced, and sequences of biosynthetic genes encoding MSDINs (cyclic peptide precursor) and prolyl oligopeptidases (POPA and POPB) were obtained. The two Amanita species yielded 29 and 18 MSDINs, respectively. In addition, two MSDIN sequences were cloned from L. brunneoincarnata basidiomes. The toxin MSDIN genes encoding amatoxins or phallotoxins from the three genera were compared, and a phylogenetic tree constructed. Prolyl oligopeptidase B (POPB), a key enzyme in the biosynthetic pathway, was used in phylogenetic reconstruction to infer the evolutionary history of the genes. Phylogenies of POPB and POPA based on both coding and amino acid sequences showed very different results: while POPA genes clearly reflected the phylogeny of the host species, POPB did not; strikingly, it formed a wellsupported monophyletic clade, despite that the species belong to different genera in disjunct families. POPA, a known house-keeping gene, was shown to be restricted in a branch containing only Amanita species and the phylogeny resembled that of those Amanita species. Phylogenetic analyses of MSDIN and POPB genes showed tight coordination and disjunct distribution. A POPB gene tree was compared with a corresponding species tree, and distances and substitution rates were compared. The result suggested POPB genes have significant smaller distances and rates than the house-keeping rpb2, discounting massive gene loss. Under this assumption, the incongruency between the gene tree and species tree was shown with strong support. Additionally, k-mer analyses consistently cluster Galerina and Amanita POPB genes, while Lepiota POPB is distinct. Our result suggests that horizontal gene transfer (HGT), at least between Amanita and Galerina, was involved in the acquisition of POPB genes, which may shed light on the evolution of the α-amanitin biosynthetic pathway.
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Amanita cingulata a new annulate species of Amanita sect vaginatae from subtropical china
Phytotaxa, 2017Co-Authors: Jianwei Liu, Qing Cai, Yangyang Cui, Zhu L YangAbstract:Amanita cingulata , a new species of Amanita sect. Vaginatae with an annulus on the stipe is described based on morphological and molecular evidence. This species was found in forests dominated by members of Fagaceae and/or Pinaceae and is characterized by its small basidioma with a white pileus covered with white and lumpy volval remnants, a pileal margin with short striations, a white fragile annulus, a weak and easily broken lumpy volva on the stipe base and ellipsoid to elongate basidiospores measuring 9–11.5 × 5.5–7 μm. The new species is compared with similar species, especially the annulate taxa of A. sect. Vaginatae.
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multi locus phylogeny of lethal Amanitas implications for species diversity and historical biogeography
BMC Evolutionary Biology, 2014Co-Authors: Rodham E. Tulloss, Li P Tang, Bau Tolgor, Ping Zhang, Zuo H Chen, Zhu L YangAbstract:Lethal Amanitas (Amanita section Phalloideae) are a group of wild, fatal mushrooms causing many poisoning cases worldwide. However, the diversity and evolutionary history of these lethal mushrooms remain poorly known due to the limited sampling and insufficient gene fragments employed for phylogenetic analyses. In this study, five gene loci (nrLSU, ITS, rpb2, ef1-α and β-tubulin) with a widely geographic sampling from East and South Asia, Europe, North and Central America, South Africa and Australia were analysed with maximum-likelihood, maximum-parsimony and Bayesian inference methods. Biochemical analyses were also conducted with intention to detect amatoxins and phalloidin in 14 representative samples. Lethal Amanitas were robustly supported to be a monophyletic group after excluding five species that were provisionally defined as lethal Amanitas based on morphological studies. In lethal Amanitas, 28 phylogenetic species were recognised by integrating molecular phylogenetic analyses with morphological studies, and 14 of them represented putatively new species. The biochemical analyses indicated a single origin of cyclic peptide toxins (amatoxins and phalloidin) within Amanita and suggested that this kind of toxins seemed to be a synapomorphy of lethal Amanitas. Molecular dating through BEAST and biogeographic analyses with LAGRANGE and RASP indicated that lethal Amanitas most likely originated in the Palaeotropics with the present crown group dated around 64.92 Mya in the early Paleocene, and the East Asia–eastern North America or Eurasia–North America–Central America disjunct distribution patterns were primarily established during the middle Oligocene to Miocene. The cryptic diversity found in this study indicates that the species diversity of lethal Amanitas is strongly underestimated under the current taxonomy. The intercontinental sister species or sister groups relationships among East Asia and eastern North America or Eurasia–North America–Central America within lethal Amanitas are best explained by the diversification model of Palaeotropical origin, dispersal via the Bering Land Bridge, followed by regional vicariance speciation resulting from climate change during the middle Oligocene to the present. These findings indicate the importance of both dispersal and vicariance in shaping the intercontinental distributions of these ectomycorrhizal fungi.
Anne Pringle - One of the best experts on this subject based on the ideXlab platform.
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horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal Amanita
New Phytologist, 2015Co-Authors: Maryam Chaib De Mares, Jaqueline Hess, Dimitrios Floudas, Anna Lipzen, Cindy Choi, Megan Kennedy, Igor V Grigoriev, Anne PringleAbstract:Summary � The genus Amanita encompasses both symbiotic, ectomycorrhizal fungi and asymbiotic litter decomposers; all species are derived from asymbiotic ancestors. Symbiotic species are no longer able to degrade plant cell walls. The carbohydrate esterases family 1 (CE1s) is a diverse group of enzymes involved in carbon metabolism, including decomposition and carbon storage. CE1 genes of the ectomycorrhizal A. muscaria appear diverged from all other fungal homologues, and more similar to CE1s of bacteria, suggesting a horizontal gene transfer (HGT) event. � In order to test whether Amanita CE1s were acquired horizontally, we built a phylogeny of CE1s collected from across the tree of life, and describe the evolution of CE1 genes among Amanita and relevant lineages of bacteria. � CE1s of symbiotic Amanita were very different from CE1s of asymbiotic Amanita, and are more similar to bacterial CE1s. The protein structure of one CE1 gene of A. muscaria matched a depolymerase that degrades the carbon storage molecule poly((R)-3-hydroxybutyrate) (PHB). Asymbiotic Amanita do not carry sequence or structural homologues of these genes. � The CE1s acquired through HGT may enable novel metabolisms, or play roles in signaling or defense. This is the first evidence for the horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal fungi.
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Horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal Amanita
2014Co-Authors: Anne Pringle, Jaqueline Hess, Dimitrios Floudas, Anna Lipzen, Cindy Choi, Megan Kennedy, Igor V Grigoriev, De Mares, Maryam ChaibAbstract:metabolism, comparative genomics, evolution of symbiosis, evolutionary novelty, horizontal gene transfer (HGT), saprotrophy. Summary The genus Amanita encompasses both symbiotic, ectomycorrhizal fungi and asymbiotic litter decomposers; all species are derived from asymbiotic ancestors. Symbiotic species are no longer able to degrade plant cell walls. The carbohydrate esterases family 1 (CE1s) is a diverse group of enzymes involved in carbon metabolism, including decomposition and carbon stor-age. CE1 genes of the ectomycorrhizal A. muscaria appear diverged from all other fungal ho-mologues, and more similar to CE1s of bacteria, suggesting a horizontal gene transfer (HGT) event. In order to test whether Amanita CE1s were acquired horizontally, we built a phylogeny of CE1s collected from across the tree of life, and describe the evolution of CE1 genes among Amanita and relevant lineages of bacteria. CE1s of symbiotic Amanita were very different from CE1s of asymbiotic Amanita, and are more similar to bacterial CE1s. The protein structure of one CE1 gene of A. muscaria matched a depolymerase that degrades the carbon storage molecule poly((R)-3-hydroxybutyrate) (PHB). Asymbiotic Amanita do not carry sequence or structural homologues of these genes. The CE1s acquired through HGT may enable novel metabolisms, or play roles in signaling or defense. This is the first evidence for the horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal fungi
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A sampling of the diversity of color and form within the genus Amanita.
2012Co-Authors: Benjamin E. Wolfe, Rodham E. Tulloss, Anne PringleAbstract:Clockwise, from top left, Amanita muscaria subsp. flavivolvata, Amanita frostiana, Amanita jacksonii, an undescribed Amanita species, the saprotrophic Amanita manicata, and Amanita phalloides.
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using the incomplete genome of the ectomycorrhizal fungus Amanita bisporigera to identify molecular polymorphisms in the related Amanita phalloides
Molecular Ecology Notes, 2006Co-Authors: Rachel I Adams, Heather E Hallen, Anne PringleAbstract:We describe the cross-genomic isolation of 13 single nucleotide polymorphisms (SNPs) and one variable microsatellite from five loci for the death cap mushroom Amanita phalloides. Microsatellite repeats were identified by searching the partial Amanita bisporigera genome. Flanking primers were designed for 25 of these microsatellite loci and tested for cross-amplification in A. phalloides. One locus contained an interrupted, compound microsatellite, and four loci contained one to six SNPs. These results demonstrate the usefulness of even an incomplete genome to identify molecular markers for population studies in nonmodel organisms.
Maryam Chaib De Mares - One of the best experts on this subject based on the ideXlab platform.
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horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal Amanita
New Phytologist, 2015Co-Authors: Maryam Chaib De Mares, Jaqueline Hess, Dimitrios Floudas, Anna Lipzen, Cindy Choi, Megan Kennedy, Igor V Grigoriev, Anne PringleAbstract:Summary � The genus Amanita encompasses both symbiotic, ectomycorrhizal fungi and asymbiotic litter decomposers; all species are derived from asymbiotic ancestors. Symbiotic species are no longer able to degrade plant cell walls. The carbohydrate esterases family 1 (CE1s) is a diverse group of enzymes involved in carbon metabolism, including decomposition and carbon storage. CE1 genes of the ectomycorrhizal A. muscaria appear diverged from all other fungal homologues, and more similar to CE1s of bacteria, suggesting a horizontal gene transfer (HGT) event. � In order to test whether Amanita CE1s were acquired horizontally, we built a phylogeny of CE1s collected from across the tree of life, and describe the evolution of CE1 genes among Amanita and relevant lineages of bacteria. � CE1s of symbiotic Amanita were very different from CE1s of asymbiotic Amanita, and are more similar to bacterial CE1s. The protein structure of one CE1 gene of A. muscaria matched a depolymerase that degrades the carbon storage molecule poly((R)-3-hydroxybutyrate) (PHB). Asymbiotic Amanita do not carry sequence or structural homologues of these genes. � The CE1s acquired through HGT may enable novel metabolisms, or play roles in signaling or defense. This is the first evidence for the horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal fungi.
Edgardo Alberto - One of the best experts on this subject based on the ideXlab platform.
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especies toxicas de agaricales halladas en la argentina nueva cita de Amanita pantherina y reevaluacion de la comestibilidad de tricholoma equestre
Boletin de la Sociedad Argentina de Botanica, 2008Co-Authors: Bernardo E Lechner, Edgardo AlbertoAbstract:Summary: Poisonous species of Agaricales found in Argentina: new record of Amanita pantherina and revaluation of the edibility of Tricholoma equestre. Mushrooms are not usually included in the traditional eating habits of the Argentinean population. Nevertheless, there are some people that consume wild species. This practice has caused poisoning to important number of adults and children over the last years. In the present paper we describe the poisonous mushroom Amanita pantherina as a new record for Argentina and we provide a key for the identification of the species of Amanita belonging to Secc. Amanita found in our country. We also describe and explain a new poisoning syndrome caused by Tricholoma equestre. This species was recently prohibited in some countries of Europe.
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Especies tóxicas de Agaricales halladas en la Argentina: nueva cita de Amanita pantherina y reevaluación de la comestibilidad de Tricholoma equestre Poisonous species of Agaricales found in Argentina: new record of Amanita pantherina and revaluation
Sociedad Argentina de Botánica, 2008Co-Authors: Bernardo E Lechner, Edgardo AlbertoAbstract:Si bien los hongos comestibles en la Argentina no constituyen una parte importante de la dieta, algunas personas colectan y consumen especies silvestres. Esto ha ocasionado en los últimos años la intoxicación de un número importante de adultos y niños. En el presente trabajo se cita por primera vez para la Argentina el hongo tóxico Amanita pantherina, se describen las colecciones halladas y se provee una clave para la determinación de las especies de Amanita Secc. Amanita. A su vez se describe y se reevalúa la comestibilidad de Tricholoma equestre causante de un nuevo síndrome de intoxicación, por lo cual fue recientemente prohibido en varios países de la comunidad Europea.Mushrooms are not usually included in the traditional eating habits of the Argentinean population. Nevertheless, there are some people that consume wild species. This practice has caused poisoning to important number of adults and children over the last years. In the present paper we describe the poisonous mushroom Amanita pantherina as a new record for Argentina and we provide a key for the identification of the species of Amanita belonging to Secc. Amanita found in our country. We also describe and explain a new poisoning syndrome caused by Tricholoma equestre. This species was recently prohibited in some countries of Europe
Jaqueline Hess - One of the best experts on this subject based on the ideXlab platform.
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horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal Amanita
New Phytologist, 2015Co-Authors: Maryam Chaib De Mares, Jaqueline Hess, Dimitrios Floudas, Anna Lipzen, Cindy Choi, Megan Kennedy, Igor V Grigoriev, Anne PringleAbstract:Summary � The genus Amanita encompasses both symbiotic, ectomycorrhizal fungi and asymbiotic litter decomposers; all species are derived from asymbiotic ancestors. Symbiotic species are no longer able to degrade plant cell walls. The carbohydrate esterases family 1 (CE1s) is a diverse group of enzymes involved in carbon metabolism, including decomposition and carbon storage. CE1 genes of the ectomycorrhizal A. muscaria appear diverged from all other fungal homologues, and more similar to CE1s of bacteria, suggesting a horizontal gene transfer (HGT) event. � In order to test whether Amanita CE1s were acquired horizontally, we built a phylogeny of CE1s collected from across the tree of life, and describe the evolution of CE1 genes among Amanita and relevant lineages of bacteria. � CE1s of symbiotic Amanita were very different from CE1s of asymbiotic Amanita, and are more similar to bacterial CE1s. The protein structure of one CE1 gene of A. muscaria matched a depolymerase that degrades the carbon storage molecule poly((R)-3-hydroxybutyrate) (PHB). Asymbiotic Amanita do not carry sequence or structural homologues of these genes. � The CE1s acquired through HGT may enable novel metabolisms, or play roles in signaling or defense. This is the first evidence for the horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal fungi.
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Horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal Amanita
2014Co-Authors: Anne Pringle, Jaqueline Hess, Dimitrios Floudas, Anna Lipzen, Cindy Choi, Megan Kennedy, Igor V Grigoriev, De Mares, Maryam ChaibAbstract:metabolism, comparative genomics, evolution of symbiosis, evolutionary novelty, horizontal gene transfer (HGT), saprotrophy. Summary The genus Amanita encompasses both symbiotic, ectomycorrhizal fungi and asymbiotic litter decomposers; all species are derived from asymbiotic ancestors. Symbiotic species are no longer able to degrade plant cell walls. The carbohydrate esterases family 1 (CE1s) is a diverse group of enzymes involved in carbon metabolism, including decomposition and carbon stor-age. CE1 genes of the ectomycorrhizal A. muscaria appear diverged from all other fungal ho-mologues, and more similar to CE1s of bacteria, suggesting a horizontal gene transfer (HGT) event. In order to test whether Amanita CE1s were acquired horizontally, we built a phylogeny of CE1s collected from across the tree of life, and describe the evolution of CE1 genes among Amanita and relevant lineages of bacteria. CE1s of symbiotic Amanita were very different from CE1s of asymbiotic Amanita, and are more similar to bacterial CE1s. The protein structure of one CE1 gene of A. muscaria matched a depolymerase that degrades the carbon storage molecule poly((R)-3-hydroxybutyrate) (PHB). Asymbiotic Amanita do not carry sequence or structural homologues of these genes. The CE1s acquired through HGT may enable novel metabolisms, or play roles in signaling or defense. This is the first evidence for the horizontal transfer of carbohydrate metabolism genes into ectomycorrhizal fungi