The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Kenji Miura - One of the best experts on this subject based on the ideXlab platform.
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Efficient transient protein expression in tomato cultivars and Wild Species using agroinfiltration-mediated high expression system
Plant Cell Reports, 2019Co-Authors: Ken Hoshikawa, Satoshi Fujita, Na Renhu, Kentaro Ezura, Tsuyoshi Yamamoto, Satoko Nonaka, Hiroshi Ezura, Kenji MiuraAbstract:Key message The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and Wild Species with high level of protein expression. Abstract Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana . In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and Wild Species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and Wild Species, we observed Species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and Wild Species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and Species as well as a model tomato, even though characteristics are often different among tomato cultivars or Species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
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efficient transient protein expression in tomato cultivars and Wild Species using agroinfiltration mediated high expression system
Plant Cell Reports, 2019Co-Authors: Ken Hoshikawa, Satoshi Fujita, Na Renhu, Kentaro Ezura, Tsuyoshi Yamamoto, Satoko Nonaka, Hiroshi Ezura, Kenji MiuraAbstract:The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and Wild Species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and Wild Species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and Wild Species, we observed Species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and Wild Species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and Species as well as a model tomato, even though characteristics are often different among tomato cultivars or Species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
Ken Hoshikawa - One of the best experts on this subject based on the ideXlab platform.
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Efficient transient protein expression in tomato cultivars and Wild Species using agroinfiltration-mediated high expression system
Plant Cell Reports, 2019Co-Authors: Ken Hoshikawa, Satoshi Fujita, Na Renhu, Kentaro Ezura, Tsuyoshi Yamamoto, Satoko Nonaka, Hiroshi Ezura, Kenji MiuraAbstract:Key message The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and Wild Species with high level of protein expression. Abstract Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana . In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and Wild Species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and Wild Species, we observed Species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and Wild Species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and Species as well as a model tomato, even though characteristics are often different among tomato cultivars or Species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
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efficient transient protein expression in tomato cultivars and Wild Species using agroinfiltration mediated high expression system
Plant Cell Reports, 2019Co-Authors: Ken Hoshikawa, Satoshi Fujita, Na Renhu, Kentaro Ezura, Tsuyoshi Yamamoto, Satoko Nonaka, Hiroshi Ezura, Kenji MiuraAbstract:The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and Wild Species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and Wild Species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and Wild Species, we observed Species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and Wild Species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and Species as well as a model tomato, even though characteristics are often different among tomato cultivars or Species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
Alisdair R Fernie - One of the best experts on this subject based on the ideXlab platform.
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metabolic profiling of leaves and fruit of Wild Species tomato a survey of the solanum lycopersicum complex
Journal of Experimental Botany, 2004Co-Authors: Nicolas Schauer, Dani Zamir, Alisdair R FernieAbstract:The domestication of the tomato Solanum lycopersicum and associated selective pressures eventually led to the large-fruited varieties cultivated today. S. lycopersicum varieties are generally red-fruited, but display considerable variance in fruit colour intensity, shape, and quality. The increase in productivity on cultivation is, however, somewhat offset by the narrowing of the crops genetic base which leads to increased susceptibility to biotic and abiotic stresses. Since S. lycopersicum can easily be crossed with its Wild Species relatives, this exotic germplasm can provide a valuable source for the improvement of agriculturally important traits. A GC-MS based survey is presented here of the relative metabolic levels of leaves and fruit of S. lycopersicum and five Wild Species of tomato that can be crossed with it (S. pimpinellifolium, S. neorickii, S. chmielewskii, S. habrochaites, and S. pennellii). Changes in metabolite contents were identified in the Wild Species that are potentially important with respect to stress responses, as well as in metabolites of nutritional importance. The significance of these changes is discussed with respect to the use of the various Wild Species for metabolic engineering within wide breeding strategies.
N. Sarla - One of the best experts on this subject based on the ideXlab platform.
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Yield-enhancing quantitative trait loci (QTLs) from Wild Species.
Biotechnology advances, 2007Co-Authors: B. P. Mallikarjuna Swamy, N. SarlaAbstract:Wild Species of crop plants are increasingly being used to improve various agronomic traits including yield in cultivars. Dense molecular maps have enabled mapping of quantitative trait loci (QTLs) for complex traits such as yield. QTLs for increased yield have been identified from Wild relatives of several crop plants. Advanced backcross QTL analysis has been used to identify naturally occurring favorable QTL alleles for yield and minimize the effect of unwanted alleles from Wild Species. Yield QTLs from Wild Species are distributed on almost all chromosomes but more often in some regions. Many QTLs for yield and related traits derived from different Wild accessions or Species map to identical chromosomal regions. QTLs for highly correlated yield associated traits are also often co-located implying linkage or pleiotropic effects. Many QTLs have been detected in more than one environment and in more than one genetic background. The overall direction of effect of some QTLs however, may vary with genetic context. Thus, there is evidence of stable and consistent major effect yield-enhancing QTLs derived from Wild Species in several crops. Such QTLs are good targets for use in marker assisted selection though their context-dependency is a major constraint. Literature on yield QTLs mapped from Wild Species is summarized with special reference to rice and tomato.
Hiroshi Ezura - One of the best experts on this subject based on the ideXlab platform.
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Efficient transient protein expression in tomato cultivars and Wild Species using agroinfiltration-mediated high expression system
Plant Cell Reports, 2019Co-Authors: Ken Hoshikawa, Satoshi Fujita, Na Renhu, Kentaro Ezura, Tsuyoshi Yamamoto, Satoko Nonaka, Hiroshi Ezura, Kenji MiuraAbstract:Key message The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and Wild Species with high level of protein expression. Abstract Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana . In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and Wild Species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and Wild Species, we observed Species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and Wild Species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and Species as well as a model tomato, even though characteristics are often different among tomato cultivars or Species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
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efficient transient protein expression in tomato cultivars and Wild Species using agroinfiltration mediated high expression system
Plant Cell Reports, 2019Co-Authors: Ken Hoshikawa, Satoshi Fujita, Na Renhu, Kentaro Ezura, Tsuyoshi Yamamoto, Satoko Nonaka, Hiroshi Ezura, Kenji MiuraAbstract:The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and Wild Species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and Wild Species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and Wild Species, we observed Species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and Wild Species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and Species as well as a model tomato, even though characteristics are often different among tomato cultivars or Species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
