The Experts below are selected from a list of 606 Experts worldwide ranked by ideXlab platform

Stefan Kunz - One of the best experts on this subject based on the ideXlab platform.

  • site 1 protease is required for proteolytic processing of the glycoproteins of the south American Hemorrhagic Fever viruses junin machupo and guanarito
    Journal of Virology, 2008
    Co-Authors: Jillian M Rojek, Andrew M Lee, Ngocthao Nguyen, Christina F Spiropoulou, Stefan Kunz
    Abstract:

    The cellular proprotein convertase site 1 protease (S1P) has been implicated in the proteolytic processing of the glycoproteins (GPs) of Old World arenaviruses. Here we report that S1P is also involved in the processing of the GPs of the genetically more-distant South American Hemorrhagic Fever viruses Guanarito, Machupo, and Junin. Efficient cleavage of Guanarito virus GP, whose protease recognition sites deviate from the reported S1P consensus sequence, indicates a broader specificity of S1P than anticipated. Lack of GP processing of Junin virus dramatically reduced production of infectious virus and prevented cell-to-cell propagation. Infection of S1P-deficient cells resulted in viral persistence over several weeks without the emergence of escape variants able to use other cellular proteases for GP processing.

  • characterization of the cellular receptors for the south American Hemorrhagic Fever viruses junin guanarito and machupo
    Virology, 2006
    Co-Authors: Jillian M Rojek, Christina F Spiropoulou, Stefan Kunz
    Abstract:

    Abstract The New World arenaviruses Junin, Machupo, and Guanarito are the causative agents of Hemorrhagic Fevers (HF) with high mortality in humans. The cellular receptor for Old World arenaviruses and one subgroup of the New World arenaviruses (Clade C) have been identified as α-dystroglycan (α-DG). In contrast, the receptor(s) of the South American HF viruses, which belong to the Clade B New World arenaviruses, are currently unknown. To begin to characterize the cellular receptors used by these pathogens, we generated recombinant retroviral pseudotypes with the glycoproteins of Guanarito, Junin, and Machupo. Infection with the South American HF viruses is independent of α-DG and functional receptors for Guanarito, Junin, and Machupo were found on most human cell types and cells derived from non-human primate and rodents. Guanarito, Junin, and Machupo share a common receptor, which is distinct from the receptor(s) used by the closely related non-pathogenic Clade B virus Amapari, and the genetically more distant Clade A and C New World arenaviruses. We show that the cellular receptor(s) for the South American HF viruses are proteins or protein-linked entities and that infection is not dependent on protein-linked N -glycans, O -glycans, or glycosaminoglycans.

Tony Schountz - One of the best experts on this subject based on the ideXlab platform.

  • Transcriptome sequencing and annotation for the Jamaican fruit bat (Artibeus jamaicensis
    2016
    Co-Authors: Timothy I. Shaw, Anuj Srivastava, Wen-chi Chou, Liang Liu, Ann Hawkinson, Travis C. Glenn, Rick Adams, Tony Schountz
    Abstract:

    The Jamaican fruit bat (Artibeus jamaicensis) is one of the most common bats in the tropical Americas. It is thought to be a potential reservoir host of Tacaribe virus, an arenavirus closely related to the South American Hemorrhagic Fever viruses. We performed transcriptome sequencing and annotation from lung, kidney and spleen tissues using 454 and Illumina platforms to develop this species as an animal model. More than 100,000 contigs were assembled, with 25,000 genes that were functionally annotated. Of the remaining unannotated contigs, 80 % were found within bat genomes or transcriptomes. Annotated genes are involved in a broad range of activities ranging from cellular metabolism to genome regulation through ncRNAs. Reciprocal BLAST best hits yielded 8,785 sequences that are orthologous to mouse, rat, cattle, horse and human. Species tree analysis of sequences from 2,378 loci was used to achieve 95 % bootstrap support for the placement of bat as sister to the clade containing horse, dog, and cattle. Through substitution rate estimation between bat and human, 32 genes were identified with evidence for positive selection. We also identified 466 immune-related genes, which may be useful for studying Tacaribe virus infection of this species. The Jamaican fruit bat transcriptome dataset is a resource that should provide additional candidate markers for studying bat evolution and ecology, and tools for analysis of the hos

  • Transcriptome sequencing and annotation for the Jamaican fruit bat (Artibeus jamaicensis).
    PloS one, 2012
    Co-Authors: Timothy I. Shaw, Anuj Srivastava, Wen-chi Chou, Liang Liu, Ann Hawkinson, Travis C. Glenn, Rick A. Adams, Tony Schountz
    Abstract:

    The Jamaican fruit bat (Artibeus jamaicensis) is one of the most common bats in the tropical Americas. It is thought to be a potential reservoir host of Tacaribe virus, an arenavirus closely related to the South American Hemorrhagic Fever viruses. We performed transcriptome sequencing and annotation from lung, kidney and spleen tissues using 454 and Illumina platforms to develop this species as an animal model. More than 100,000 contigs were assembled, with 25,000 genes that were functionally annotated. Of the remaining unannotated contigs, 80% were found within bat genomes or transcriptomes. Annotated genes are involved in a broad range of activities ranging from cellular metabolism to genome regulation through ncRNAs. Reciprocal BLAST best hits yielded 8,785 sequences that are orthologous to mouse, rat, cattle, horse and human. Species tree analysis of sequences from 2,378 loci was used to achieve 95% bootstrap support for the placement of bat as sister to the clade containing horse, dog, and cattle. Through substitution rate estimation between bat and human, 32 genes were identified with evidence for positive selection. We also identified 466 immune-related genes, which may be useful for studying Tacaribe virus infection of this species. The Jamaican fruit bat transcriptome dataset is a resource that should provide additional candidate markers for studying bat evolution and ecology, and tools for analysis of the host response and pathology of disease.

Timothy I. Shaw - One of the best experts on this subject based on the ideXlab platform.

  • Transcriptome sequencing and annotation for the Jamaican fruit bat (Artibeus jamaicensis
    2016
    Co-Authors: Timothy I. Shaw, Anuj Srivastava, Wen-chi Chou, Liang Liu, Ann Hawkinson, Travis C. Glenn, Rick Adams, Tony Schountz
    Abstract:

    The Jamaican fruit bat (Artibeus jamaicensis) is one of the most common bats in the tropical Americas. It is thought to be a potential reservoir host of Tacaribe virus, an arenavirus closely related to the South American Hemorrhagic Fever viruses. We performed transcriptome sequencing and annotation from lung, kidney and spleen tissues using 454 and Illumina platforms to develop this species as an animal model. More than 100,000 contigs were assembled, with 25,000 genes that were functionally annotated. Of the remaining unannotated contigs, 80 % were found within bat genomes or transcriptomes. Annotated genes are involved in a broad range of activities ranging from cellular metabolism to genome regulation through ncRNAs. Reciprocal BLAST best hits yielded 8,785 sequences that are orthologous to mouse, rat, cattle, horse and human. Species tree analysis of sequences from 2,378 loci was used to achieve 95 % bootstrap support for the placement of bat as sister to the clade containing horse, dog, and cattle. Through substitution rate estimation between bat and human, 32 genes were identified with evidence for positive selection. We also identified 466 immune-related genes, which may be useful for studying Tacaribe virus infection of this species. The Jamaican fruit bat transcriptome dataset is a resource that should provide additional candidate markers for studying bat evolution and ecology, and tools for analysis of the hos

  • Transcriptome sequencing and annotation for the Jamaican fruit bat (Artibeus jamaicensis).
    PloS one, 2012
    Co-Authors: Timothy I. Shaw, Anuj Srivastava, Wen-chi Chou, Liang Liu, Ann Hawkinson, Travis C. Glenn, Rick A. Adams, Tony Schountz
    Abstract:

    The Jamaican fruit bat (Artibeus jamaicensis) is one of the most common bats in the tropical Americas. It is thought to be a potential reservoir host of Tacaribe virus, an arenavirus closely related to the South American Hemorrhagic Fever viruses. We performed transcriptome sequencing and annotation from lung, kidney and spleen tissues using 454 and Illumina platforms to develop this species as an animal model. More than 100,000 contigs were assembled, with 25,000 genes that were functionally annotated. Of the remaining unannotated contigs, 80% were found within bat genomes or transcriptomes. Annotated genes are involved in a broad range of activities ranging from cellular metabolism to genome regulation through ncRNAs. Reciprocal BLAST best hits yielded 8,785 sequences that are orthologous to mouse, rat, cattle, horse and human. Species tree analysis of sequences from 2,378 loci was used to achieve 95% bootstrap support for the placement of bat as sister to the clade containing horse, dog, and cattle. Through substitution rate estimation between bat and human, 32 genes were identified with evidence for positive selection. We also identified 466 immune-related genes, which may be useful for studying Tacaribe virus infection of this species. The Jamaican fruit bat transcriptome dataset is a resource that should provide additional candidate markers for studying bat evolution and ecology, and tools for analysis of the host response and pathology of disease.

Jillian M Rojek - One of the best experts on this subject based on the ideXlab platform.

  • site 1 protease is required for proteolytic processing of the glycoproteins of the south American Hemorrhagic Fever viruses junin machupo and guanarito
    Journal of Virology, 2008
    Co-Authors: Jillian M Rojek, Andrew M Lee, Ngocthao Nguyen, Christina F Spiropoulou, Stefan Kunz
    Abstract:

    The cellular proprotein convertase site 1 protease (S1P) has been implicated in the proteolytic processing of the glycoproteins (GPs) of Old World arenaviruses. Here we report that S1P is also involved in the processing of the GPs of the genetically more-distant South American Hemorrhagic Fever viruses Guanarito, Machupo, and Junin. Efficient cleavage of Guanarito virus GP, whose protease recognition sites deviate from the reported S1P consensus sequence, indicates a broader specificity of S1P than anticipated. Lack of GP processing of Junin virus dramatically reduced production of infectious virus and prevented cell-to-cell propagation. Infection of S1P-deficient cells resulted in viral persistence over several weeks without the emergence of escape variants able to use other cellular proteases for GP processing.

  • characterization of the cellular receptors for the south American Hemorrhagic Fever viruses junin guanarito and machupo
    Virology, 2006
    Co-Authors: Jillian M Rojek, Christina F Spiropoulou, Stefan Kunz
    Abstract:

    Abstract The New World arenaviruses Junin, Machupo, and Guanarito are the causative agents of Hemorrhagic Fevers (HF) with high mortality in humans. The cellular receptor for Old World arenaviruses and one subgroup of the New World arenaviruses (Clade C) have been identified as α-dystroglycan (α-DG). In contrast, the receptor(s) of the South American HF viruses, which belong to the Clade B New World arenaviruses, are currently unknown. To begin to characterize the cellular receptors used by these pathogens, we generated recombinant retroviral pseudotypes with the glycoproteins of Guanarito, Junin, and Machupo. Infection with the South American HF viruses is independent of α-DG and functional receptors for Guanarito, Junin, and Machupo were found on most human cell types and cells derived from non-human primate and rodents. Guanarito, Junin, and Machupo share a common receptor, which is distinct from the receptor(s) used by the closely related non-pathogenic Clade B virus Amapari, and the genetically more distant Clade A and C New World arenaviruses. We show that the cellular receptor(s) for the South American HF viruses are proteins or protein-linked entities and that infection is not dependent on protein-linked N -glycans, O -glycans, or glycosaminoglycans.

Salas R - One of the best experts on this subject based on the ideXlab platform.

  • Latin American Hemorrhagic Fever.
    Infectious disease clinics of North America, 1994
    Co-Authors: Vainrub B, Salas R
    Abstract:

    Several viral and bacterial agents are responsible for Hemorrhagic Fever in Latin America, but there are three agents that are only present in this region: (1) the Junin virus, (2) the Machupo virus, and (3) the Guanaritovirus, members of the Tacaribe complex of the family Arenaviridae and responsible for the Argentinian, Bolivian, and Venezuelan Hemorrhagic Fever. The clinical manifestations of Hemorrhagic Fever are similar for the three viruses, mainly myalgia, arthralgia, headache, relative bradycardia, conjunctivitis, nausea, vomiting, diarrhea, and Hemorrhagic disorders. In this article, the three viruses, their causes, and manifestations are discussed.