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Angeli Kodjo – One of the best experts on this subject based on the ideXlab platform.

  • serotypes a1 and a2 of mannheimia haemolytica are susceptible to genotypic capsular and phenotypic variations in contrast to t3 and t4 serotypes of bibersteinia pasteurella trehalosi
    Fems Microbiology Letters, 2008
    Co-Authors: Laurence Villard, Dominique Gauthier, Frana Oise Maurin, Evelyne Borges, Yves Richard, Georgette Abadie, Angeli Kodjo

    Abstract:

    Mannheimia haemolytica and Bibersteinia ( Pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. The disease is determined by specific serotypes found in cattle and small ruminants. The molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. This study aimed to detect the instability and variations of bacterial strains that may affect the Analysis of epidemic strains, or the stability of vaccinal strains. Eight strains of M. haemolytica belonging to serotypes A1 and A2 and three B. trehalosi strains of the T3 and T4 serotypes were used. Strains were subjected to pulsed field gel electrophoresis (PFGE) and capsular and phenotypic typing at each round of a total of 50 successive subcultures. Remarkable stability was found in all selected strains of B. trehalosi in contrast to M. haemoltyica , in which strains of both serotypes showed pattern variations produced by PFGE and capsular and phenotypic Analysis. Objective criteria for M. haemolytica and B. trehalosi typing are consequently addressed.

  • SerotypesA1andA2of Mannheimia haemolyticaare susceptible to genotypic, capsularand phenotypic variations in contrast toT3 and T4 serotypes of Bibersteinia (Pasteurella) trehalosi
    Veterinary Journal, 2006
    Co-Authors: Laurence Villard, Dominique Gauthier, Evelyne Borges, Yves Richard, Georgette Abadie, A. Lacheretz, Françoise Maurin, Angeli Kodjo

    Abstract:

    Mannheimia haemolytica and Bibersteinia (Pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. The disease is determined by specific serotypes found in cattle and small ruminants. The molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. This study aimed to detect the instability and variations of bacterial strains that may affect the Analysis of epidemic strains, or the stability of vaccinal strains. Eight strains of M. haemolytica belonging to serotypes A1 and A2 and three B. trehalosi strains of the T3 and T4 serotypes were used. Strains were subjected to pulsed field gel electrophoresis (PFGE) and capsular and phenotypic typing at each round of a total of 50 successive subcultures. Remarkable stability was found in all selected strains of B. trehalosi in contrast to M. haemoltyica, in which strains of both serotypes showed pattern variations produced by PFGE and capsular and phenotypic Analysis. Objective criteria for M. haemolytica and B. trehalosi typing are consequently addressed.

Rae Woong Park – One of the best experts on this subject based on the ideXlab platform.

  • The Tissue Microarray Object Model: A Data Model for Storage, Analysis, and Exchange of Tissue Microarray Experimental Data
    Archives of Pathology & Laboratory Medicine, 2006
    Co-Authors: Yu Rang Park, Rae Woong Park

    Abstract:

    Abstract Context.—Tissue microarray (TMA) is an array-based technology allowing the examination of hundreds of tissue samples on a single slide. To handle, exchange, and disseminate TMA data, we need standard representations of the methods used, of the data generated, and of the clinical and histopathologic information related to TMA data Analysis. Objective.—To create a comprehensive data model with flexibility that supports diverse experimental designs and with expressivity and extensibility that enables an adequate and comprehensive description of new clinical and histopathologic data elements. Design.—We designed a tissue microarray object model (TMA-OM). Both the array information and the experimental procedure models are created by referring to the microarray gene expression object model, minimum information specification for in situ hybridization and immunohistochemistry experiments, and the TMA data exchange specifications. The clinical and histopathologic information model is created by using Col…

Judith Breuer – One of the best experts on this subject based on the ideXlab platform.

  • Use of genomic Analysis of varicella-zoster virus to investigate suspected varicella-zoster transmission within a renal unit.
    Journal of Clinical Virology, 2006
    Co-Authors: P. Molyneaux, S. P. Parker, I.h. Khan, C.g.m. Millar, Judith Breuer

    Abstract:

    Abstract Background The source of hospital-acquired chickenpox infection may be presumed from a known exposure, but has not been previously proven using genomic Analysis. Objective Investigation of suspected VZV transmission was done using single nucleotide polymorphism genomic Analysis. Study design Comparison was made of viral isolates from two patients with chickenpox on the same ward who were not known to have had direct contact. Results An identical genotype in the variable R1 region of the VZV was isolated from the two patients. Conclusion Inapparent hospital-acquired transmission was the most likely route of infection.