The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Gerhard Schlosser - One of the best experts on this subject based on the ideXlab platform.

  • evolution of the hypoxia sensitive cells involved in amniote respiratory reflexes
    eLife, 2017
    Co-Authors: Dorit Hockman, Ela W Knapik, Gerhard Schlosser, Alan J Burns, Keith P Gates, Benjamin Jevans, Alessandro Mongera, Shannon Fisher, Gokhan Unlu, Charles K Kaufman
    Abstract:

    The evolutionary origins of the hypoxia-sensitive cells that trigger amniote respiratory reflexes - carotid body glomus cells, and 'pulmonary neuroendocrine cells' (PNECs) - are obscure. Homology has been proposed between glomus cells, which are neural crest-derived, and the hypoxia-sensitive 'neuroepithelial cells' (NECs) of fish gills, whose embryonic origin is unknown. NECs have also been likened to PNECs, which differentiate in situ within lung airway epithelia. Using genetic lineage-tracing and neural crest-deficient mutants in zebrafish, and physical fate-mapping in frog and lamprey, we find that NECs are not neural crest-derived, but endoderm-derived, like PNECs, whose endodermal origin we confirm. We discover neural crest-derived catecholaminergic cells associated with zebrafish pharyngeal arch blood vessels, and propose a new model for amniote hypoxia-sensitive cell evolution: endoderm-derived NECs were retained as PNECs, while the carotid body evolved via the aggregation of neural crest-derived catecholaminergic (chromaffin) cells already associated with blood vessels in anamniote pharyngeal arches.

  • Secondary neurogenesis in the brain of the African clawed frog, Xenopus laevis, as revealed by PCNA, Delta-1, Neurogenin-related-1, and NeuroD expression.
    The Journal of comparative neurology, 2005
    Co-Authors: Mario F Wullimann, Philippe Vernier, Elke Rink, Gerhard Schlosser
    Abstract:

    After primary neurogenesis in the Xenopus laevis embryo, a massive new surge of neurogenesis and related neurogenic and proneural gene expression occurs in the spinal cord at the beginning of the larval period (starting at Stage 46), which corresponds to well-documented secondary neurogenesis in larval zebrafish central nervous system development. Here, we document related neural proliferation and gene expression patterns in the brain of Xenopus, in various embryonic and larval stages, showing the distribution of proliferative cells (immunostaining of cells containing the proliferating cell nuclear antigen; the auxiliary protein of DNA polymerase δ; PCNA), and the activity of some critical genes expressed during neurogenesis (i.e., Delta-1, Neurogenin-related-1, NeuroD). This study reveals that the early larval stage in Xenopus (Stage 48) displays patterns of proliferation (PCNA), as well as of neurogenic (Delta-1) and proneural (Ngnr-1; NeuroD) gene expression that are qualitatively almost identical to those seen in the 3-day postembryonic zebrafish or the 12.5/13.5-day embryonic mouse. Furthermore, a comparable bauplan of early proliferation zones (including their neuromeric organization) as described in the postembryonic zebrafish apparently exists in tetrapods (Xenopus). Altogether, the data presented suggest a common brain bauplan on the level of early proliferation patterns and neurogenic/proneural gene activity in Anamniotes, if not vertebrates. J. Comp. Neurol. 489:387–402, 2005. © 2005 Wiley-Liss, Inc.

  • Secondary neurogenesis in the brain of the African clawed frog, Xenopus laevis, as revealed by PCNA, Delta-1, Neurogenin-related-1, and NeuroD expression.
    Journal of Comparative Neurology, 2005
    Co-Authors: Mario F. Wullimann, Philippe Vernier, Elke Rink, Gerhard Schlosser
    Abstract:

    After primary neurogenesis in the Xenopus laevis embryo, a massive new surge of neurogenesis and related neurogenic and proneural gene expression occurs in the spinal cord at the beginning of the larval period (starting at Stage 46), which corresponds to well-documented secondary neurogenesis in larval zebrafish central nervous system development. Here, we document related neural proliferation and gene expression patterns in the brain of Xenopus, in various embryonic and larval stages, showing the distribution of proliferative cells (immunostaining of cells containing the proliferating cell nuclear antigen; the auxiliary protein of DNA polymerase delta; PCNA), and the activity of some critical genes expressed during neurogenesis (i.e., Delta-1, Neurogenin-related-1, NeuroD). This study reveals that the early larval stage in Xenopus (Stage 48) displays patterns of proliferation (PCNA), as well as of neurogenic (Delta-1) and proneural (Ngnr-1; NeuroD) gene expression that are qualitatively almost identical to those seen in the 3-day postembryonic zebrafish or the 12.5/13.5-day embryonic mouse. Furthermore, a comparable bauplan of early proliferation zones (including their neuromeric organization) as described in the postembryonic zebrafish apparently exists in tetrapods (Xenopus). Altogether, the data presented suggest a common brain bauplan on the level of early proliferation patterns and neurogenic/proneural gene activity in Anamniotes, if not vertebrates..

Agustin Gonzalez - One of the best experts on this subject based on the ideXlab platform.

  • identification of striatal and pallidal regions in the subpallium of Anamniotes
    Brain Behavior and Evolution, 2014
    Co-Authors: Agustin Gonzalez, Ruth Morona, Nerea Moreno, Sandra Bandin, Jesus M Lopez
    Abstract:

    The telencephalic basal ganglia (BG) of amniotes consist of two subdivisions, striatum and pallidum, which share many features, including development, cell types, neurotransmitter organization and hodology. In particular, these two subdivisions during development are defined on the basis of discrete gene expression patterns (genoarchitecture or genoarchitectonics). The characterization of the BG in the subpallium of representatives of the different classes of anamniote vertebrates was first approached in studies dealing with their localization, hodology and main neurochemical characteristics. Thus, it was proposed that an impressive degree of conservation exists across species. New insights can be gained by the comparative analysis of the expression of conserved transcription factors that distinctly define the striatal and pallidal components of the BG in all vertebrates. In addition, the expression of other genes that characterize neighboring regions of the BG is also useful to define the boundaries of each subdivision. Following this approach, we have analyzed the BG in the brain of juvenile representatives of amphibians, lungfishes, holosteans, Polypteriformes and Chondrichthyes. In addition, we briefly review previous data in teleosts and agnathans. The markers used include islet 1 and Dlx as striatal markers, whereas Nkx2.1 is essential for the identification of the pallidum. In turn, Pax6 and in particular Tbr1 are expressed in the pallium. These markers have been systematically analyzed in combination with neuronal markers of specific subpallial territories and cell populations, such as tyrosine hydroxylase, γ-aminobutyric acid, nitric oxide synthase, substance P and enkephalin. The results highlight that many genes share common distribution patterns and are arranged in conserved combinations whose identification has served to define homologies between components of the BG in distant species.

  • distribution of choline acetyltransferase immunoreactivity in the brain of anuran rana perezi xenopus laevis and urodele pleurodeles waltl amphibians
    The Journal of Comparative Neurology, 1997
    Co-Authors: Oscar Marin, Wilhelmus J. A. J. Smeets, Agustin Gonzalez
    Abstract:

    Because our knowledge of cholinergic systems in the brains of amphibians is limited, the present study aimed to provide detailed information on the distribution of cholinergic cell bodies and fibers as revealed by immunohistochemistry with antibodies directed against the enzyme choline acetyltransferase (ChAT). To determine general and derived features of the cholinergic systems within the class of Amphibia, both anuran (Rana perezi, Xenopus laevis) and urodele (Pleurodeles waltl) amphibians were studied. Distinct groups of ChAT-immunoreactive cell bodies were observed in the basal telencephalon, hypothalamus, habenula, isthmic nucleus, isthmic reticular formation, cranial nerve motor nuclei, and spinal cord. Prominent plexuses of cholinergic fibers were found in the olfactory bulb, pallium, basal telencephalon, ventral thalamus, tectum, and nucleus interpeduncularis. Comparison of these results with those obtained in other vertebrates, including a segmental approach to correlate cell populations, reveals that the cholinergic systems in amphibians share many features with amniotes. Thus, cholinergic pedunculopontine and laterodorsal tegmental nuclei could be identified in the amphibian brain. The finding of weakly immunoreactive cells in the striatum of Rana, which is in contrast with the condition found in Xenopus, Pleurodeles, and other Anamniotes studied so far, has revived the notion that basal ganglia organization is more preserved during evolution than previously thought. J. Comp. Neurol. 382:499-534, 1997. © 1997 Wiley-Liss Inc.

  • nitric oxide synthase in the brain of a urodele amphibian pleurodeles waltl and its relation to catecholaminergic neuronal structures
    Brain Research, 1996
    Co-Authors: Agustin Gonzalez, Alberto Munoz, M Munoz, O Maŕin, R Arevalo, Angel Porteros, Jose R Alonso
    Abstract:

    The neuronal structures with NADPH-diaphorase activity and nitric oxide synthase (NOS) immunoreactivity have been studied in the brain of the urodele amphibian Pleurodeles waltl by means of histochemical and immunocytochemical techniques. Both approaches resulted in the selective labeling of the same neurons and fiber tracts in the brain, except for the primary olfactory fibers that did not stain for NOS but were positive for NADPH-diaphorase. NOS-containing neurons were found in the olfactory bulbs, pallial regions, septum, caudal striatum, amygdala and preoptic area. Only a few diencephalic cells were labeled in the posterior tubercle and ventral hypothalamus. In the brainstem, abundant cells were labeled in the tectum, mesencephalic tegmentum and isthmic region. The most conspicuous cell population was found in the isthmic-pretrigeminal region. Particularly well stained cells were distributed throughout the rhombencephalon in areas related to the descending trigeminal tract, solitary tract, raphe nucleus and the mid-caudal reticular formation. In the cervical spinal cord, NOS-containing cells were present in the dorsal, intermediate and ventral grey fields. Cells in the preoptic, postotic and dorsal root ganglia were also labeled. Double labeling techniques revealed an extensive codistribution of neurons with NOS and catecholamines in the urodele brain but actual colocalization in the same cells was never observed. The organization of the central systems in urodeles with NOS appears to share many features not only with other Anamniotes but also with amniotes.

Mario F Wullimann - One of the best experts on this subject based on the ideXlab platform.

  • the long adventurous journey of rhombic lip cells in jawed vertebrates a comparative developmental analysis
    Frontiers in Neuroanatomy, 2011
    Co-Authors: Mario F Wullimann, Thomas Mueller, Martin Distel, Andreas Babaryka, Benedikt Grothe, Reinhard W Koster
    Abstract:

    This review summarizes vertebrate rhombic lip and early cerebellar development covering classic approaches up to modern developmental genetics which identifies the relevant differential gene expression domains and their progeny. Most of this information is derived from amniotes. However, progress in Anamniotes, particularly in the zebrafish, has recently been made. The current picture suggests that rhombic lip and cerebellar development in jawed vertebrates (gnathostomes) share many characteristics. Regarding cerebellar development, these include a ptf1a expressing ventral cerebellar proliferation (VCP) giving rise to Purkinje cells and other inhibitory cerebellar cell types, and an atoh1 expressing upper rhombic lip giving rise to an external granular layer (EGL, i.e., excitatory granule cells) and an early ventral migration into the anterior rhombencephalon (cholinergic nuclei). As for the lower rhombic lip (LRL), gnathostome commonalities likely include the formation of precerebellar nuclei (mossy fiber origins) and partially primary auditory nuclei (likely convergently evolved) from the atoh1 expressing dorsal zone. The fate of the ptf1a expressing ventral LRL zone which gives rise to (excitatory cells of) the inferior olive (climbing fiber origin) and (inhibitory cells of ) cochlear nuclei in amniotes, has not been determined in Anamniotes. Special for the zebrafish in comparison to amniotes is the predominant origin of anamniote excitatory deep cerebellar nuclei homologues (i.e., eurydendroid cells) from ptf1a expressing VCP cells, the sequential activity of various atoh1 paralogues and the incomplete coverage of the subpial cerebellar plate with proliferative EGL cells. Nevertheless, the conclusion that a rhombic lip and its major derivatives evolved with gnathostome vertebrates only and are thus not an ancestral craniate character complex is supported by the absence of a cerebellum (and likely absence of its afferent and efferent nuclei) in jawless fishes.

  • the long adventurous journey of rhombic lip cells in jawed vertebrates
    Frontiers in Neuroanatomy, 2011
    Co-Authors: Mario F Wullimann, Thomas Mueller, Martin Distel, Andreas Babaryka, Benedikt Grothe, Reinhard W Koster
    Abstract:

    This review summarizes vertebrate rhombic lip and early cerebellar development covering classic approaches up to modern developmental genetics which identifies the relevant differential gene expression domains and their progeny. Most of this information is derived from amniotes. However, progress in Anamniotes, particularly in the zebrafish, has recently been made. The current picture suggests that rhombic lip and cerebellar development in jawed vertebrates (gnathostomes) share many characteristics. Regarding cerebellar development, these include a ptf1a expressing ventral cerebellar proliferation (VCP) giving rise to Purkinje cells and other inhibitory cerebellar cell types, and an atoh1 expressing upper rhombic lip giving rise to an external granular layer (EGL, i.e., excitatory granule cells) and an early ventral migration into the anterior rhombencephalon (cholinergic nuclei). As for the lower rhombic lip (LRL), gnathostome commonalities likely include the formation of precerebellar nuclei (mossy fiber origins) and partially primary auditory nuclei (likely convergently evolved) from the atoh1 expressing dorsal zone. The fate of the ptf1a expressing ventral LRL zone which gives rise to (excitatory cells of) the inferior olive (climbing fiber origin) and (inhibitory cells of ) cochlear nuclei in amniotes, has not been determined in Anamniotes. Special for the zebrafish in comparison to amniotes is the predominant origin of anamniote excitatory deep cerebellar nuclei homologs (i.e., eurydendroid cells) from ptf1a expressing VCP cells, the sequential activity of various atoh1 paralogs and the incomplete coverage of the subpial cerebellar plate with proliferative EGL cells. Nevertheless, the conclusion that a rhombic lip and its major derivatives evolved with gnathostome vertebrates only and are thus not an ancestral craniate character complex is supported by the absence of a cerebellum (and likely absence of its afferent and efferent nuclei) in jawless fishes.

  • Secondary neurogenesis in the brain of the African clawed frog, Xenopus laevis, as revealed by PCNA, Delta-1, Neurogenin-related-1, and NeuroD expression.
    The Journal of comparative neurology, 2005
    Co-Authors: Mario F Wullimann, Philippe Vernier, Elke Rink, Gerhard Schlosser
    Abstract:

    After primary neurogenesis in the Xenopus laevis embryo, a massive new surge of neurogenesis and related neurogenic and proneural gene expression occurs in the spinal cord at the beginning of the larval period (starting at Stage 46), which corresponds to well-documented secondary neurogenesis in larval zebrafish central nervous system development. Here, we document related neural proliferation and gene expression patterns in the brain of Xenopus, in various embryonic and larval stages, showing the distribution of proliferative cells (immunostaining of cells containing the proliferating cell nuclear antigen; the auxiliary protein of DNA polymerase δ; PCNA), and the activity of some critical genes expressed during neurogenesis (i.e., Delta-1, Neurogenin-related-1, NeuroD). This study reveals that the early larval stage in Xenopus (Stage 48) displays patterns of proliferation (PCNA), as well as of neurogenic (Delta-1) and proneural (Ngnr-1; NeuroD) gene expression that are qualitatively almost identical to those seen in the 3-day postembryonic zebrafish or the 12.5/13.5-day embryonic mouse. Furthermore, a comparable bauplan of early proliferation zones (including their neuromeric organization) as described in the postembryonic zebrafish apparently exists in tetrapods (Xenopus). Altogether, the data presented suggest a common brain bauplan on the level of early proliferation patterns and neurogenic/proneural gene activity in Anamniotes, if not vertebrates. J. Comp. Neurol. 489:387–402, 2005. © 2005 Wiley-Liss, Inc.

Ray Keller - One of the best experts on this subject based on the ideXlab platform.

  • Large, long range tensile forces drive convergence during Xenopus blastopore closure and body axis elongation
    eLife, 2018
    Co-Authors: David R. Shook, Eric M Kasprowicz, Lance A. Davidson, Ray Keller
    Abstract:

    Indirect evidence suggests that blastopore closure during gastrulation of Anamniotes, including amphibians such as Xenopus laevis, depends on circumblastoporal convergence forces generated by the marginal zone (MZ), but direct evidence is lacking. We show that explanted MZs generate tensile convergence forces up to 1.5 μN during gastrulation and over 4 μN thereafter. These forces are generated by convergent thickening (CT) until the midgastrula and increasingly by convergent extension (CE) thereafter. Explants from ventralized embryos, which lack tissues expressing CE but close their blastopores, produce up to 2 μN of tensile force, showing that CT alone generates forces sufficient to close the blastopore. Uniaxial tensile stress relaxation assays show stiffening of mesodermal and ectodermal tissues around the onset of neurulation, potentially enhancing long-range transmission of convergence forces. These results illuminate the mechanobiology of early vertebrate morphogenic mechanisms, aid interpretation of phenotypes, and give insight into the evolution of blastopore closure mechanisms.

Jean-leon Thomas - One of the best experts on this subject based on the ideXlab platform.

  • rna profiling of the human and mouse spinal cord stem cell niches reveals an embryonic like regionalization with msx1 roof plate derived cells
    Stem cell reports, 2019
    Co-Authors: Hussein Ghazale, Chantal Ripoll, Nicolas Leventoux, Laurent Jacob, Safa Azar, Daria Mamaeva, Yael Glasson, Charles-felix Calvo, Jean-leon Thomas
    Abstract:

    Summary Anamniotes, rodents, and young humans maintain neural stem cells in the ependymal zone (EZ) around the central canal of the spinal cord, representing a possible endogenous source for repair in mammalian lesions. Cell diversity and genes specific for this region are ill defined. A cellular and molecular resource is provided here for the mouse and human EZ based on RNA profiling, immunostaining, and fluorescent transgenic mice. This uncovered the conserved expression of 1,200 genes including 120 transcription factors. Unexpectedly the EZ maintains an embryonic-like dorsal-ventral pattern of expression of spinal cord developmental transcription factors (ARX, FOXA2, MSX1, and PAX6). In mice, dorsal and ventral EZ cells express Vegfr3 and are derived from the embryonic roof and floor plates. The dorsal EZ expresses a high level of Bmp6 and Gdf10 genes and harbors a subpopulation of radial quiescent cells expressing MSX1 and ID4 transcription factors.

  • RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells
    Elsevier, 2019
    Co-Authors: Hussein Ghazale, Chantal Ripoll, Nicolas Leventoux, Laurent Jacob, Safa Azar, Daria Mamaeva, Yael Glasson, Charles-felix Calvo, Jean-leon Thomas, Sarah Meneceur
    Abstract:

    Summary: Anamniotes, rodents, and young humans maintain neural stem cells in the ependymal zone (EZ) around the central canal of the spinal cord, representing a possible endogenous source for repair in mammalian lesions. Cell diversity and genes specific for this region are ill defined. A cellular and molecular resource is provided here for the mouse and human EZ based on RNA profiling, immunostaining, and fluorescent transgenic mice. This uncovered the conserved expression of 1,200 genes including 120 transcription factors. Unexpectedly the EZ maintains an embryonic-like dorsal-ventral pattern of expression of spinal cord developmental transcription factors (ARX, FOXA2, MSX1, and PAX6). In mice, dorsal and ventral EZ cells express Vegfr3 and are derived from the embryonic roof and floor plates. The dorsal EZ expresses a high level of Bmp6 and Gdf10 genes and harbors a subpopulation of radial quiescent cells expressing MSX1 and ID4 transcription factors. : A niche of stem cells is present around the central canal of the adult spinal cord. A better description of cell diversity and genes expressed in this niche may help to use it to promote spinal cord regeneration after lesions. In this article, based on several techniques, Ghazale and colleagues provide a cellular and molecular resource for the adult human and mouse stem cell niches. Keywords: spinal cord, niche, neural stem cells, regionalization, ependyma, ependymal cells, radial glial cells, transcription factors, Msx1, roof plate, floor plat