The Experts below are selected from a list of 210 Experts worldwide ranked by ideXlab platform
Tobias Huth - One of the best experts on this subject based on the ideXlab platform.
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sea anemone toxin atx ii elicits a fiber dependent pain and enhances resurgent and persistent sodium currents in large sensory neurons
Molecular Pain, 2012Co-Authors: Alexandra B Klinger, Christian Alzheimer, Andrea S Link, Lisa K Kutsche, Ruth Sittl, Theresa E Schuy, Tal Hoffmann, Mirjam Eberhardt, Barbara Namer, Tobias HuthAbstract:Background Gain-of-function mutations of the nociceptive voltage-gated sodium channel Nav1.7 lead to inherited pain syndromes, such as paroxysmal extreme pain disorder (PEPD). One characteristic of these mutations is slowed fast-inactivation kinetics, which may give rise to resurgent sodium currents. It is long known that toxins from Anemonia sulcata, such as ATX-II, slow fast inactivation and skin contact for example during diving leads to various symptoms such as pain and itch. Here, we investigated if ATX-II induces resurgent currents in sensory neurons of the dorsal root ganglion (DRGs) and how this may translate into human sensations.
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sea anemone toxin atx ii elicits a fiber dependent pain and enhances resurgent and persistent sodium currents in large sensory neurons
Molecular Pain, 2012Co-Authors: Alexandra B Klinger, Christian Alzheimer, Andrea S Link, Lisa K Kutsche, Ruth Sittl, Theresa E Schuy, Tal Hoffmann, Mirjam Eberhardt, Barbara Namer, Tobias HuthAbstract:Background Gain-of-function mutations of the nociceptive voltage-gated sodium channel Nav1.7 lead to inherited pain syndromes, such as paroxysmal extreme pain disorder (PEPD). One characteristic of these mutations is slowed fast-inactivation kinetics, which may give rise to resurgent sodium currents. It is long known that toxins from Anemonia sulcata, such as ATX-II, slow fast inactivation and skin contact for example during diving leads to various symptoms such as pain and itch. Here, we investigated if ATX-II induces resurgent currents in sensory neurons of the dorsal root ganglion (DRGs) and how this may translate into human sensations.
Paola Furla - One of the best experts on this subject based on the ideXlab platform.
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conspicuous morphological differentiation without speciation in Anemonia viridis cnidaria actiniaria
Systematics and Biodiversity, 2018Co-Authors: Cedric Mallien, Paola Furla, Barbara Porro, Thamilla Zamoum, Caroline Olivier, Jorg Wiedenmann, Didier ForcioliAbstract:Anemonia viridis is a model species for studies of physiological and transcriptomic response to symbiosis and environmental stress (temperature, light, symbiosis breakdown). Five morphs are describ...
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comprehensive est analysis of the symbiotic sea anemone Anemonia viridis
BMC Genomics, 2009Co-Authors: Cecile Sabourault, Denis Allemand, Philippe Ganot, Emeline Deleury, Paola FurlaAbstract:Background Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts and unicellular dinoflagellate algae, known as zooxanthellae. These algae are photosynthetic and the cnidarian-zooxanthellae association is based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks between the partners. To better characterize symbiotic relationships between a cnidarian host and its dinoflagellate symbionts, we conducted a large-scale EST study on a symbiotic sea anemone, Anemonia viridis, in which the two tissue layers (epiderm and gastroderm) can be easily separated.
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comprehensive est analysis of the symbiotic sea anemone Anemonia viridis
BMC Genomics, 2009Co-Authors: Cecile Sabourault, Denis Allemand, Philippe Ganot, Emeline Deleury, Paola FurlaAbstract:Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts and unicellular dinoflagellate algae, known as zooxanthellae. These algae are photosynthetic and the cnidarian-zooxanthellae association is based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks between the partners. To better characterize symbiotic relationships between a cnidarian host and its dinoflagellate symbionts, we conducted a large-scale EST study on a symbiotic sea anemone, Anemonia viridis, in which the two tissue layers (epiderm and gastroderm) can be easily separated. A single cDNA library was constructed from symbiotic tissue of sea anemones A. viridis in various environmental conditions (both normal and stressed). We generated 39,939 high quality ESTs, which were assembled into 14,504 unique sequences (UniSeqs). Sequences were analysed and sorted according to their putative origin (animal, algal or bacterial). We identified many new repeated elements in the 3'UTR of most animal genes, suggesting that these elements potentially have a biological role, especially with respect to gene expression regulation. We identified genes of animal origin that have no homolog in the non-symbiotic starlet sea anemone Nematostella vectensis genome, but in other symbiotic cnidarians, and may therefore be involved in the symbiosis relationship in A. viridis. Comparison of protein domain occurrence in A. viridis with that in N. vectensis demonstrated an increase in abundance of some molecular functions, such as protein binding or antioxidant activity, suggesting that these functions are essential for the symbiotic state and may be specific adaptations. This large dataset of sequences provides a valuable resource for future studies on symbiotic interactions in Cnidaria. The comparison with the closest available genome, the sea anemone N. vectensis, as well as with EST datasets from other symbiotic cnidarians provided a set of candidate genes involved in symbiosis-related molecular crosstalks. Altogether, these results provide new molecular insights that could be used as a starting-point for further functional genomics studies.
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oxidative stress and apoptotic events during thermal stress in the symbiotic sea anemone Anemonia viridis
FEBS Journal, 2006Co-Authors: Sophie Richier, Denis Allemand, Cecile Sabourault, Juliette Courtiade, Nathalie Zucchini, Paola FurlaAbstract:Symbiosis between cnidarian and photosynthetic protists is widely distributed over temperate and tropical seas. These symbioses can periodically breakdown, a phenomenon known as cnidarian bleaching. This event can be irreversible for some associations subjected to acute and/or prolonged environmental disturbances, and leads to the death of the animal host. During bleaching, oxidative stress has been described previously as acting at molecular level and apoptosis is suggested to be one of the mechanisms involved. We focused our study on the role of apoptosis in bleaching via oxidative stress in the association between the sea anemone Anemonia viridis and the dinoflagellates Symbiodinium species. Characterization of caspase-like enzymes were conducted at the biochemical and molecular level to confirm the presence of a caspase-dependent apoptotic phenomenon in the cnidarian host. We provide evidence of oxidative stress followed by induction of caspase-like activity in animal host cells after an elevated temperature stress, suggesting the concomitant action of these components in bleaching.
Alexandra B Klinger - One of the best experts on this subject based on the ideXlab platform.
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sea anemone toxin atx ii elicits a fiber dependent pain and enhances resurgent and persistent sodium currents in large sensory neurons
Molecular Pain, 2012Co-Authors: Alexandra B Klinger, Christian Alzheimer, Andrea S Link, Lisa K Kutsche, Ruth Sittl, Theresa E Schuy, Tal Hoffmann, Mirjam Eberhardt, Barbara Namer, Tobias HuthAbstract:Background Gain-of-function mutations of the nociceptive voltage-gated sodium channel Nav1.7 lead to inherited pain syndromes, such as paroxysmal extreme pain disorder (PEPD). One characteristic of these mutations is slowed fast-inactivation kinetics, which may give rise to resurgent sodium currents. It is long known that toxins from Anemonia sulcata, such as ATX-II, slow fast inactivation and skin contact for example during diving leads to various symptoms such as pain and itch. Here, we investigated if ATX-II induces resurgent currents in sensory neurons of the dorsal root ganglion (DRGs) and how this may translate into human sensations.
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sea anemone toxin atx ii elicits a fiber dependent pain and enhances resurgent and persistent sodium currents in large sensory neurons
Molecular Pain, 2012Co-Authors: Alexandra B Klinger, Christian Alzheimer, Andrea S Link, Lisa K Kutsche, Ruth Sittl, Theresa E Schuy, Tal Hoffmann, Mirjam Eberhardt, Barbara Namer, Tobias HuthAbstract:Background Gain-of-function mutations of the nociceptive voltage-gated sodium channel Nav1.7 lead to inherited pain syndromes, such as paroxysmal extreme pain disorder (PEPD). One characteristic of these mutations is slowed fast-inactivation kinetics, which may give rise to resurgent sodium currents. It is long known that toxins from Anemonia sulcata, such as ATX-II, slow fast inactivation and skin contact for example during diving leads to various symptoms such as pain and itch. Here, we investigated if ATX-II induces resurgent currents in sensory neurons of the dorsal root ganglion (DRGs) and how this may translate into human sensations.
Lászlo Béress - One of the best experts on this subject based on the ideXlab platform.
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biologically active polypeptides of Anemonia sulcata and of other sea anemones tools in the study of exitable membranes
Journal of Toxicology-toxin Reviews, 2005Co-Authors: Lászlo BéressAbstract:During the past thirty years sea anemones turned out to be important producer of biologically highly active polypeptides acting on Na+ and K+ ion channels. They are compoused from 27 to 59 aminoacids having molecular weights from 3000 to 7000 Da. All contain six cystein molecules which are interconnected to three sisulfide bridges. Because of their specifique mode of action on nerve cell exitation and on the mammalian heart muscle they became very important tools in neurophysiology and pharmacology. In the present review the research on sea anemone polypeptid toxins is summarized from a historical point of view, focussed on the polypeptides of Anemonia sulcata, Condylactis gigantea and Anthopleura elegantissima.
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A simple biochemical method in the search for bioactive polypeptides in a sea anemone (Anemonia sulcata).
Toxicon, 1996Co-Authors: J. Sanchez, Thomas Bruhn, Abel Aneiros, E. Wachter, Lászlo BéressAbstract:The sea anemone Anemonia sulcata is a well-known natural source of supply of biologically active polypeptides. So far, five toxins, ATX I, II, III, IV and AS V, several polyvalent protease inhibitors, an elastase inhibitor, two blood pressure-depressive polypeptides and very recently peptides that inhibit competitively the binding of 125I-dendrotoxin to rat brain membranes and block the voltage-sensitive K+ channels, have been isolated from it. The sea anemone toxins (especially toxin II of A. sulcata, ATX II) are very important tools in neurophysiological and pharmacological research, and their structure-function relationship has been investigated. Because of the great scientific value of the sea anemone toxins a simplification of their purification procedure was elaborated.
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Kalicludines and kaliseptine. Two different classes of sea anemone toxins for voltage sensitive K+ channels.
Journal of Biological Chemistry, 1995Co-Authors: Hugues Schweitz, Lászlo Béress, Thomas Bruhn, Eric Guillemare, Danielle Moinier, Jean-marc Lancelin, Michel LazdunskiAbstract:Abstract New peptides have been isolated from the sea anemone Anemonia sulcata which inhibit competitively the binding of 125I-dendrotoxin I (a classical ligand for K+ channel) to rat brain membranes and behave as blockers of voltage-sensitive K+ channels. Sea anemone kalicludines are 58-59-amino acid peptides cross-linked with three disulfide bridges. They are structurally homologous both to dendrotoxins which are snake venom toxins and to the basic pancreatic trypsin inhibitor (Kunitz inhibitor) and have the unique property of expressing both the function of dendrotoxins in blocking voltage-sensitive K+ channels and the function of the Kunitz inhibitor in inhibiting trypsin. Kaliseptine is another structural class of peptide comprising 36 amino acids with no sequence homology with kalicludines or with dendrotoxins. In spite of this structural difference, it binds to the same receptor site as dendrotoxin and kalicludines and is as efficient as a K+ channel inhibitor as the most potent kalicludine.
Emeline Deleury - One of the best experts on this subject based on the ideXlab platform.
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comprehensive est analysis of the symbiotic sea anemone Anemonia viridis
BMC Genomics, 2009Co-Authors: Cecile Sabourault, Denis Allemand, Philippe Ganot, Emeline Deleury, Paola FurlaAbstract:Background Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts and unicellular dinoflagellate algae, known as zooxanthellae. These algae are photosynthetic and the cnidarian-zooxanthellae association is based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks between the partners. To better characterize symbiotic relationships between a cnidarian host and its dinoflagellate symbionts, we conducted a large-scale EST study on a symbiotic sea anemone, Anemonia viridis, in which the two tissue layers (epiderm and gastroderm) can be easily separated.
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comprehensive est analysis of the symbiotic sea anemone Anemonia viridis
BMC Genomics, 2009Co-Authors: Cecile Sabourault, Denis Allemand, Philippe Ganot, Emeline Deleury, Paola FurlaAbstract:Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts and unicellular dinoflagellate algae, known as zooxanthellae. These algae are photosynthetic and the cnidarian-zooxanthellae association is based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks between the partners. To better characterize symbiotic relationships between a cnidarian host and its dinoflagellate symbionts, we conducted a large-scale EST study on a symbiotic sea anemone, Anemonia viridis, in which the two tissue layers (epiderm and gastroderm) can be easily separated. A single cDNA library was constructed from symbiotic tissue of sea anemones A. viridis in various environmental conditions (both normal and stressed). We generated 39,939 high quality ESTs, which were assembled into 14,504 unique sequences (UniSeqs). Sequences were analysed and sorted according to their putative origin (animal, algal or bacterial). We identified many new repeated elements in the 3'UTR of most animal genes, suggesting that these elements potentially have a biological role, especially with respect to gene expression regulation. We identified genes of animal origin that have no homolog in the non-symbiotic starlet sea anemone Nematostella vectensis genome, but in other symbiotic cnidarians, and may therefore be involved in the symbiosis relationship in A. viridis. Comparison of protein domain occurrence in A. viridis with that in N. vectensis demonstrated an increase in abundance of some molecular functions, such as protein binding or antioxidant activity, suggesting that these functions are essential for the symbiotic state and may be specific adaptations. This large dataset of sequences provides a valuable resource for future studies on symbiotic interactions in Cnidaria. The comparison with the closest available genome, the sea anemone N. vectensis, as well as with EST datasets from other symbiotic cnidarians provided a set of candidate genes involved in symbiosis-related molecular crosstalks. Altogether, these results provide new molecular insights that could be used as a starting-point for further functional genomics studies.