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Seifollah Bahramikia - One of the best experts on this subject based on the ideXlab platform.
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efficacy of different fractions of Anethum graveolens leaves on serum lipoproteins and serum and liver oxidative status in experimentally induced hypercholesterolaemic rat models
The American Journal of Chinese Medicine, 2009Co-Authors: Seifollah Bahramikia, Razieh YazdanparastAbstract:Over the last few years, many studies have focused on plants with therapeutic properties. Anethum graveolens L. (Umbilliferae) is used as an anti-hypercholesterolaemic plant in Iranian folk medicine. This study was to disclose the antioxidant and hypolipidaemic properties of different fractions of the Anethum graveolens extract (AGE), evaluated in hypercholesterolaemic rats. The animals were divided into five groups, Group I served as healthy control; groups II to V were made hypercholesterolaemic by feeding high-fat diet (HFD) for 3 weeks. After induction of hypercholesterolaemia, the rats in groups III to V received different fractions [diethyl ether (EEF), ethyl acetate (EAF) and water (WF) fractions, respectively] of the AGE at a dose of 50 mg/kg body weight per day for 2 weeks. Feeding HFD increased serum total cholesterol (TC), triglyceride (TG) and LDL-cholesterol significantly in Group II as compared to Group I. Treatments of rats with fractions EEF, EAF and WF caused a significant decrease in TC,...
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efficacy of different fractions of Anethum graveolens leaves on serum lipoproteins and serum and liver oxidative status in experimentally induced hypercholesterolaemic rat models
The American Journal of Chinese Medicine, 2009Co-Authors: Seifollah Bahramikia, Razieh YazdanparastAbstract:Over the last few years, many studies have focused on plants with therapeutic properties. Anethum graveolens L. (Umbilliferae) is used as an anti-hypercholesterolaemic plant in Iranian folk medicine. This study was to disclose the antioxidant and hypolipidaemic properties of different fractions of the Anethum graveolens extract (AGE), evaluated in hypercholesterolaemic rats. The animals were divided into five groups, Group I served as healthy control; groups II to V were made hypercholesterolaemic by feeding high-fat diet (HFD) for 3 weeks. After induction of hypercholesterolaemia, the rats in groups III to V received different fractions [diethyl ether (EEF), ethyl acetate (EAF) and water (WF) fractions, respectively] of the AGE at a dose of 50 mg/kg body weight per day for 2 weeks. Feeding HFD increased serum total cholesterol (TC), triglyceride (TG) and LDL-cholesterol significantly in Group II as compared to Group I. Treatments of rats with fractions EEF, EAF and WF caused a significant decrease in TC, TG and LDL-C levels by (53.8, 49.9 and 42.3%), (51.5, 38.45 and 30.5%) and (54.8, 51.4 and 41.8%), respectively, however the HDL-cholesterol level was increased. In an effort to gain some knowledge about the mechanism of action, the effects of different fractions on oxidative stress induced by HFD were also investigated. Treatment with different fractions of AGE significantly increased hepatic antioxidant system activities such as SOD, CAT and GSH, along with decreased lipid peroxidation in HFD treated rats. Among these fractions, EEF showed the highest and WF showed the lowest increase in the above mentioned factors. Based on these results, it can be concluded that different fractions of AGE especially EEF, besides its hypolipidaemic property, could protect the liver against the HFD-induced oxidative damage in rats.
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antioxidant and free radical scavenging activities of different fractions of Anethum graveolens leaves using in vitro models
2008Co-Authors: Seifollah Bahramikia, Razieh YazdanparastAbstract:Summary The protective effects of fruits and vegetables against chronic diseases have been in part attributed to the presence of antioxidants in these foods. Anethum graveolens L. (dill) [Umbelliferae] is used in Iranian folk medicine as an anti-hypercholesterolaemic plant. To clarify the mode of action, primarily the antioxidant potency of the leaf crude extract and its different fractions (diethyl ether, ethyl acetate and water) were investigated, employing various established in vitro systems such as the ferric reducing antioxidant power (FRAP) and 2, 2′-azinobis 3-ethylbenzothiazoline-6sulfonate (ABTS) assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) /nitric oxide radical scavenging, iron ion chelating activity and inhibitory effect on Fe 2+ /ascorbate induced lipid peroxidation in rat liver homogenate. In the above assays, crude extract and all the fractions showed antioxidant potential to varying degrees. Among these fractions, ethyl acetate fraction exhibited higher antioxidant potency compared to the other fractions. The ethyl acetate fraction also exhibited the highest anti-hyperlipidaemic effects in animal models. These data clearly indicate that A. graveolens especially its ethyl acetate fraction possess potent antioxidant property, and this might account for its anti-hyperlipidaemic and anti-hypercholesterolaemic effects. Kew words: Anethum graveolens, Antioxidant potency, Ferric reducing antioxidant power, Lipid peroxidation, Nitric oxide radical scavenging.
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evaluation of the effect of Anethum graveolens l crude extracts onserum lipids and lipoproteins profiles in hypercholesterolaemic rats
DARU, 2008Co-Authors: Yazdan R Parast, Seifollah BahramikiaAbstract:Background and the purpose of study: Anethum graveolens L . (Umbilliferae) is used in Iranian folk medicine as an anti-hypercholesterolaemic plant. The present study was carried out to determine the effect of Anethum graveolens extract (AGE) on serum lipoproteins in hypercholesterolaemic rats and also to determine its mechanism of action to some extant on liver hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase activity. Methods: The changes in serum triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were measured using enzymatic kits. Hepatic HMG-CoA reductase activity was indirectly measured by the HMG CoA/mevalonate ratio. The amount of total phenolic and flavonoid compounds were also determined by common colorimetric methods. Results: Keeping the rats on a high-fat diet for 7 consecutive days increased the serum TC, TG and LDL-C levels significantly. However, the HDL-C level decreased relative to normal rats. Treatment of hyperlipidaemic rats with AGE (single daily dose of 1 ml, equivalent to 500 mg of the plant powder) and high-fat diet for up to 10 and/or 30 days reversed the serum lipid levels compared to rats which were fed only high-fat diet. In addition, our data indicated significant increase in HMG-CoA/mevalonate ratio as compared to rats which were fed high-fat diet after treatment with AGE for 30 days, indicating a decrease in the enzyme activity. Experiments showed that AGE has the phenolic and flavonoid content of 105.2 mg of gallic acid equivalents/g of the dried extract and 58.2 mg of catechin equivalents/g of the dried extract, respectively. Conclusion: The cumulative results clearly indicate that A. graveolens possesses potent hypocholesterolaemic effects in rats probably mediated through the suppression of endogenous cholesterol biosynthesis by inhibition of the activity of HMG-CoA reductase.
Razieh Yazdanparast - One of the best experts on this subject based on the ideXlab platform.
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efficacy of different fractions of Anethum graveolens leaves on serum lipoproteins and serum and liver oxidative status in experimentally induced hypercholesterolaemic rat models
The American Journal of Chinese Medicine, 2009Co-Authors: Seifollah Bahramikia, Razieh YazdanparastAbstract:Over the last few years, many studies have focused on plants with therapeutic properties. Anethum graveolens L. (Umbilliferae) is used as an anti-hypercholesterolaemic plant in Iranian folk medicine. This study was to disclose the antioxidant and hypolipidaemic properties of different fractions of the Anethum graveolens extract (AGE), evaluated in hypercholesterolaemic rats. The animals were divided into five groups, Group I served as healthy control; groups II to V were made hypercholesterolaemic by feeding high-fat diet (HFD) for 3 weeks. After induction of hypercholesterolaemia, the rats in groups III to V received different fractions [diethyl ether (EEF), ethyl acetate (EAF) and water (WF) fractions, respectively] of the AGE at a dose of 50 mg/kg body weight per day for 2 weeks. Feeding HFD increased serum total cholesterol (TC), triglyceride (TG) and LDL-cholesterol significantly in Group II as compared to Group I. Treatments of rats with fractions EEF, EAF and WF caused a significant decrease in TC,...
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efficacy of different fractions of Anethum graveolens leaves on serum lipoproteins and serum and liver oxidative status in experimentally induced hypercholesterolaemic rat models
The American Journal of Chinese Medicine, 2009Co-Authors: Seifollah Bahramikia, Razieh YazdanparastAbstract:Over the last few years, many studies have focused on plants with therapeutic properties. Anethum graveolens L. (Umbilliferae) is used as an anti-hypercholesterolaemic plant in Iranian folk medicine. This study was to disclose the antioxidant and hypolipidaemic properties of different fractions of the Anethum graveolens extract (AGE), evaluated in hypercholesterolaemic rats. The animals were divided into five groups, Group I served as healthy control; groups II to V were made hypercholesterolaemic by feeding high-fat diet (HFD) for 3 weeks. After induction of hypercholesterolaemia, the rats in groups III to V received different fractions [diethyl ether (EEF), ethyl acetate (EAF) and water (WF) fractions, respectively] of the AGE at a dose of 50 mg/kg body weight per day for 2 weeks. Feeding HFD increased serum total cholesterol (TC), triglyceride (TG) and LDL-cholesterol significantly in Group II as compared to Group I. Treatments of rats with fractions EEF, EAF and WF caused a significant decrease in TC, TG and LDL-C levels by (53.8, 49.9 and 42.3%), (51.5, 38.45 and 30.5%) and (54.8, 51.4 and 41.8%), respectively, however the HDL-cholesterol level was increased. In an effort to gain some knowledge about the mechanism of action, the effects of different fractions on oxidative stress induced by HFD were also investigated. Treatment with different fractions of AGE significantly increased hepatic antioxidant system activities such as SOD, CAT and GSH, along with decreased lipid peroxidation in HFD treated rats. Among these fractions, EEF showed the highest and WF showed the lowest increase in the above mentioned factors. Based on these results, it can be concluded that different fractions of AGE especially EEF, besides its hypolipidaemic property, could protect the liver against the HFD-induced oxidative damage in rats.
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antioxidant and free radical scavenging activities of different fractions of Anethum graveolens leaves using in vitro models
2008Co-Authors: Seifollah Bahramikia, Razieh YazdanparastAbstract:Summary The protective effects of fruits and vegetables against chronic diseases have been in part attributed to the presence of antioxidants in these foods. Anethum graveolens L. (dill) [Umbelliferae] is used in Iranian folk medicine as an anti-hypercholesterolaemic plant. To clarify the mode of action, primarily the antioxidant potency of the leaf crude extract and its different fractions (diethyl ether, ethyl acetate and water) were investigated, employing various established in vitro systems such as the ferric reducing antioxidant power (FRAP) and 2, 2′-azinobis 3-ethylbenzothiazoline-6sulfonate (ABTS) assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) /nitric oxide radical scavenging, iron ion chelating activity and inhibitory effect on Fe 2+ /ascorbate induced lipid peroxidation in rat liver homogenate. In the above assays, crude extract and all the fractions showed antioxidant potential to varying degrees. Among these fractions, ethyl acetate fraction exhibited higher antioxidant potency compared to the other fractions. The ethyl acetate fraction also exhibited the highest anti-hyperlipidaemic effects in animal models. These data clearly indicate that A. graveolens especially its ethyl acetate fraction possess potent antioxidant property, and this might account for its anti-hyperlipidaemic and anti-hypercholesterolaemic effects. Kew words: Anethum graveolens, Antioxidant potency, Ferric reducing antioxidant power, Lipid peroxidation, Nitric oxide radical scavenging.
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antihyperlipidaemic and antihypercholesterolaemic effects of Anethum graveolens leaves after the removal of furocoumarins
Cytobios, 2001Co-Authors: Razieh Yazdanparast, M AlaviAbstract:Serum triacylglycerides and total cholesterol levels in rats, with hyperlipidaemia induced by diet, were determined after oral adminstration of a water extract of Anethum graveolens leaves before and after the extraction of the furocoumarin content of the leaves. Administration of the extracts consecutively for 14 days reduced the triacylglycerides and total cholesterol levels by almost 50 and 20%, respectively. Chloroform extraction of furocoumarins from the aqueous extracts did not reduce the antihyperlipidaemic potential of the extracts to a significant degree. Oral administration of the essential oil of A. graveolens seeds, at two different doses, also reduced the triacylglyceride levels by almost 42%. The total cholesterol level was not reduced by the same doses of the essential oil.
Salvatore Genovese - One of the best experts on this subject based on the ideXlab platform.
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HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum graveolens, Pimpinella Anisum, and Ferulago Campestris
MDPI AG, 2019Co-Authors: Vito Alessandro Taddeo, Philippe De Medina, Serena Fiorito, Francesco Epifano, Francesca Preziuso, Nicolas Caron, Arnaud Rives, Marc Poirot, Sandrine Silvente-poirot, Salvatore GenoveseAbstract:Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely Anethum graveolens L. (dill), Pimpinella anisum L. (anise), and Ferulago campestris (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of F. campestris as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., A. graveolens)
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Modulation of the phenylpropanoid geranylation step in Anethum graveolens cultured calli by ferulic acid and umbelliferone
Industrial Crops and Products, 2018Co-Authors: Salvatore Genovese, Serena Fiorito, Vito Alessandro Taddeo, Francesco Epifano, Francesca Preziuso, Daniele FraternaleAbstract:Abstract In this paper we established the effect on the extent of the geranylation step of selected O-prenyl phenylpropanoids by their biosynthetic precursors (e.g. ferulic acid and umbelliferone) in cultured calli from Anethum graveolens L. Lyophilized vegetable material has been extracted by an overnight maceration with EtOH and 3,3-dimethylallyl, geranyl, and farnesyl derivatives of both ferulic acid and umbelliferone quantified by HPLC analysis coupled to UV/Vis detection. In no case culture media supplemented with the parent molecule provided increases in the biosynthesis of the corresponding prenylated secondary metabolite. Quite surprisingly ferulic acid and umbelliferone exerted opposite effects, the first being an elicitor of the only O-geranylation of phenylpropanoid cores while the latter was seen to largely suppress this biosynthetic step.
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quantification of biologically active o prenylated and unprenylated phenylpropanoids in dill Anethum graveolens anise pimpinella anisum and wild celery angelica archangelica
Journal of Pharmaceutical and Biomedical Analysis, 2017Co-Authors: Vito Alessandro Taddeo, Roberta Palmisano, Philippe De Medina, Salvatore Genovese, Francesco Epifano, Serena FioritoAbstract:An analytical strategy based on different extraction methodologies and HPLC with spectrophotometric (UV-vis) detection has been developed to investigate the presence of and to quantitate biologically active selected unprenylated and O-prenylated phenylpropanoids, namely umbelliferone, 4'-geranyloxyferulic acid, 7-isopentenyloxycoumarin, auraptene, and umbelliprenin in dill (Anethum graveolens L.), anise (Pimpinella anisum L.), and wild celery (Angelica archangelica L.). Absolute ethanol or 7:3 water/ethanol mixtures were seen to be the most powerful extraction solvents to perform "classic" maceration or ultrasound-assisted one in terms of yields in secondary metabolites. For Anethum and anise, umbelliprenine was found to be the most abundant prenyloxy secondary metabolite, while in wild celery 4'-geranyloxyferulic acid recorded the highest concentration. Our experimental approach demonstrated to be efficient for the simultaneous identification and quantitation of the above mentioned prenyloxyphenylpropanoids in the title plant species, that is reported herein for the first time in the literature.
Serena Fiorito - One of the best experts on this subject based on the ideXlab platform.
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HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum graveolens, Pimpinella Anisum, and Ferulago Campestris
MDPI AG, 2019Co-Authors: Vito Alessandro Taddeo, Philippe De Medina, Serena Fiorito, Francesco Epifano, Francesca Preziuso, Nicolas Caron, Arnaud Rives, Marc Poirot, Sandrine Silvente-poirot, Salvatore GenoveseAbstract:Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely Anethum graveolens L. (dill), Pimpinella anisum L. (anise), and Ferulago campestris (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of F. campestris as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., A. graveolens)
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Modulation of the phenylpropanoid geranylation step in Anethum graveolens cultured calli by ferulic acid and umbelliferone
Industrial Crops and Products, 2018Co-Authors: Salvatore Genovese, Serena Fiorito, Vito Alessandro Taddeo, Francesco Epifano, Francesca Preziuso, Daniele FraternaleAbstract:Abstract In this paper we established the effect on the extent of the geranylation step of selected O-prenyl phenylpropanoids by their biosynthetic precursors (e.g. ferulic acid and umbelliferone) in cultured calli from Anethum graveolens L. Lyophilized vegetable material has been extracted by an overnight maceration with EtOH and 3,3-dimethylallyl, geranyl, and farnesyl derivatives of both ferulic acid and umbelliferone quantified by HPLC analysis coupled to UV/Vis detection. In no case culture media supplemented with the parent molecule provided increases in the biosynthesis of the corresponding prenylated secondary metabolite. Quite surprisingly ferulic acid and umbelliferone exerted opposite effects, the first being an elicitor of the only O-geranylation of phenylpropanoid cores while the latter was seen to largely suppress this biosynthetic step.
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quantification of biologically active o prenylated and unprenylated phenylpropanoids in dill Anethum graveolens anise pimpinella anisum and wild celery angelica archangelica
Journal of Pharmaceutical and Biomedical Analysis, 2017Co-Authors: Vito Alessandro Taddeo, Roberta Palmisano, Philippe De Medina, Salvatore Genovese, Francesco Epifano, Serena FioritoAbstract:An analytical strategy based on different extraction methodologies and HPLC with spectrophotometric (UV-vis) detection has been developed to investigate the presence of and to quantitate biologically active selected unprenylated and O-prenylated phenylpropanoids, namely umbelliferone, 4'-geranyloxyferulic acid, 7-isopentenyloxycoumarin, auraptene, and umbelliprenin in dill (Anethum graveolens L.), anise (Pimpinella anisum L.), and wild celery (Angelica archangelica L.). Absolute ethanol or 7:3 water/ethanol mixtures were seen to be the most powerful extraction solvents to perform "classic" maceration or ultrasound-assisted one in terms of yields in secondary metabolites. For Anethum and anise, umbelliprenine was found to be the most abundant prenyloxy secondary metabolite, while in wild celery 4'-geranyloxyferulic acid recorded the highest concentration. Our experimental approach demonstrated to be efficient for the simultaneous identification and quantitation of the above mentioned prenyloxyphenylpropanoids in the title plant species, that is reported herein for the first time in the literature.
Stanka Damianova - One of the best experts on this subject based on the ideXlab platform.
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composition quality control and antimicrobial activity of the essential oil of long time stored dill Anethum graveolens l seeds from bulgaria
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Leopold Jirovetz, Evgenii V Georgiev, Gerhard Buchbauer, Albena Stoyanova, Stanka DamianovaAbstract:The essential oil of long-time stored seeds of dill (Anethum graveolens L.) from Bulgaria was analyzed by physicochemical methods, gas chromatography (GC), GC−mass spectrometry (MS) (achiral and chiral phases), and olfactometry, and its antimicrobial activity was tested by using different strains of microorganisms. More than 40 constituents of the essential dill oil, obtained from seeds stored for more than 35 years, could be identified as essential volatiles, responsible for the pleasant fresh (d-limonene) and spicy (d-carvone) odor of a high quality. As aroma impact compounds, d-carvone (50.1%) and d-limonene (44.1%) were found. Antimicrobial testings showed high activity of the essential A. graveolens oil against the mold Aspergillus niger and the yeasts Saccharomyces cerevisiae and Candida albicans. Keywords: Anethum graveolens; stored seeds; aroma compounds; antimicrobial testing; quality control
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composition quality control and antimicrobial activity of the essential oil of long time stored dill Anethum graveolens l seeds from bulgaria
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Leopold Jirovetz, Evgenii V Georgiev, Gerhard Buchbauer, Albena Stoyanova, Stanka DamianovaAbstract:The essential oil of long-time stored seeds of dill (Anethum graveolens L.) from Bulgaria was analyzed by physicochemical methods, gas chromatography (GC), GC-mass spectrometry (MS) (achiral and chiral phases), and olfactometry, and its antimicrobial activity was tested by using different strains of microorganisms. More than 40 constituents of the essential dill oil, obtained from seeds stored for more than 35 years, could be identified as essential volatiles, responsible for the pleasant fresh (D-limonene) and spicy (D-carvone) odor of a high quality. As aroma impact compounds, D-carvone (50.1%) and D-limonene (44.1%) were found. Antimicrobial testings showed high activity of the essential A. graveolens oil against the mold Aspergillus niger and the yeasts Saccharomyces cerevisiae and Candida albicans.