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Animal Fertility

The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform

Donald Evenson – 1st expert on this subject based on the ideXlab platform

  • Sperm chromatin structure assay for Fertility assessment.
    Current protocols in cytometry, 2020
    Co-Authors: Donald Evenson, Lorna Jost

    Abstract:

    The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. Damaged DNA in the single sperm that fertilizes the female egg can have a dramatic negative impact on fetal development. This comprehensive and detailed unit presents a rapid, reliable, practical test for DNA integrity based on staining with acridine orange. SCSA data have been conclusively shown to predict sub/inFertility. This assay is ideally suited to human and Animal Fertility clinics to assess male sperm DNA integrity as related to Fertility potential and embryo development. The authors, who have decades of experience in studying sperm viability, provide extensive commentary and methodological tips, making this unit the most detailed method for this test published to date.

  • Sperm chromatin structure assay is useful for Fertility assessment : Applications of flow cytometry across species
    , 2020
    Co-Authors: Donald Evenson, Lorna K. Jost

    Abstract:

    The Sperm Chromatin Structure Assay (SCSA) serves as a tool for measuring clinically important properties of sperm nuclear chromatin integrity. The assay utilizes the metachromatic features of Acridine Orange (AO), a DNA probe, and the principles of flow cytometry (FCM). SCSA data are not well correlated with classical sperm quality parameters and have been solidly shown to predict sub/inFertility. This assay is ideally suited to human and Animal Fertility clinics to assess male sperm DNA integrity as related to Fertility potential and embryo development as well as effects of reproductive toxicants. A detailed description of the SCSA follows.

  • Current Protocols in Cytometry – Sperm Chromatin Structure Assay for Fertility Assessment
    Current protocols in immunology, 2020
    Co-Authors: Donald Evenson, Lorna K. Jost

    Abstract:

    The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. Damaged DNA in the single sperm that fertilizes the female egg can have a dramatic negative impact on fetal development. This comprehensive and detailed unit presents a rapid, reliable, practical test for DNA integrity based on staining with acridine orange. SCSA data have been conclusively shown to predict sub/inFertility. This assay is ideally suited to human and Animal Fertility clinics to assess male sperm DNA integrity as related to Fertility potential and embryo development. The authors, who have decades of experience in studying sperm viability, provide extensive commentary and methodological tips, making this unit the most detailed method for this test published to date. Keywords: flow cytometry; sperm chromatin structure assay; SCSA; DNA denaturation; acridine orange;Animal and human Fertility; toxicology The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring

Toby Mottram – 2nd expert on this subject based on the ideXlab platform

  • Biosensor Technology addressing Agricultural Problems
    Biosystems Engineering, 2020
    Co-Authors: Maria N. Velasco-garcia, Toby Mottram

    Abstract:

    Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate sensing opens up opportunities for biosensors in many different agricultural areas -in situ analysis of pollutants in crops and soils, detection and identification of diseases in crops and livestock, on-line measurements of important food processing parameters, monitoring Animal Fertility and screening therapeutic drugs in veterinary testing. Further challenges in the commercial development of biosensors are also addressed.

  • Biosensor technology addressing agricultural problems
    Biosystems Engineering, 2003
    Co-Authors: Maria N. Velasco-garcia, Toby Mottram

    Abstract:

    Biosensor technology is a powerful alternative to conventional analytical techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diabetics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate sensing opens up opportunities for biosensors in many different agricultural areas – in situ analysis of pollutants in crops and soils, detection and identification of infectious diseases in crops and livestock, on-line measurements of important food processing parameters, monitoring Animal Fertility and screening therapeutic drugs in veterinary testing. Future challenges in the commercial development of biosensor are also addressed. © 2003 Silsoe Research Institute. All rights reserved. Published by Elsevier Science Ltd.

Lorna Jost – 3rd expert on this subject based on the ideXlab platform

  • Sperm chromatin structure assay for Fertility assessment.
    Current protocols in cytometry, 2020
    Co-Authors: Donald Evenson, Lorna Jost

    Abstract:

    The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. Damaged DNA in the single sperm that fertilizes the female egg can have a dramatic negative impact on fetal development. This comprehensive and detailed unit presents a rapid, reliable, practical test for DNA integrity based on staining with acridine orange. SCSA data have been conclusively shown to predict sub/inFertility. This assay is ideally suited to human and Animal Fertility clinics to assess male sperm DNA integrity as related to Fertility potential and embryo development. The authors, who have decades of experience in studying sperm viability, provide extensive commentary and methodological tips, making this unit the most detailed method for this test published to date.

  • Sperm chromatin structure assay is useful for Fertility assessment
    Methods in Cell Science, 2000
    Co-Authors: Donald Evenson, Lorna Jost

    Abstract:

    The Sperm Chromatin Structure Assay (SCSA) serves as a tool for measuring clinically important properties of sperm nuclear chromatin integrity. The assay utilizes the metachromatic features of Acridine Orange (AO), a DNA probe, and the principles of flow cytometry (FCM). SCSA data are not well correlated with classical sperm quality parameters and have been solidly shown to predict sub/inFertility. This assay is ideally suited to human and Animal Fertility clinics to assess male sperm DNA integrity as related to Fertility potential and embryo development as well as effects of reproductive toxicants. A detailed description of the SCSA follows.