The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Tatsuhiro Hisatsune - One of the best experts on this subject based on the ideXlab platform.
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Anserine, HClO-scavenger, protected against cognitive decline in individuals with mild cognitive impairment.
Aging, 2021Co-Authors: Nobutaka Masuoka, Chenxu Lei, Noriko Inamura, Shigenobu Shiotani, Nobuya Yanai, Kenichiro Sato, Keisuke Sakurai, Tatsuhiro HisatsuneAbstract:Neuroinflammation has been recognized as a promising target when considering strategies for treating AD. In particular, it has been shown that neutrophils and MPO-mediated neuroinflammatory responses with the production of HClO play a role in the progression of AD. In this study, we aimed to evaluate the effects of Anserine, a scavenger of HClO, on the protection of cognitive declines in persons with MCI. Fifty-eight elderly volunteers were screened, and 36 MCI individuals were assigned either to an active arm, who received 500 mg Anserine per day, or a placebo arm, for 12-weeks. To assess cognitive function, we performed MMSE at baseline and after the ingestion. The data of the MMSE for 30 subjects who completed the follow-up tests were analyzed. A significant difference was detected in the change score of MMSE between the active arm (1.9 ± 2.0; n = 15) and the placebo arm (0 ± 2.8; n = 15) (p = 0.036). After the correction with the daily intake of Anserine, the significance was elevated (p = 0.0176). Our results suggest that Anserine protects elderly persons with MCI from cognitive declines by suppressing MPO-mediated neuroinflammatory responses.
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Anserine beta alanyl 3 methyl l histidine improves neurovascular unit dysfunction and spatial memory in aged aβppswe psen1de9 alzheimer s model mice
Scientific Reports, 2017Co-Authors: Jun Kaneko, Akiko Enya, Kota Enomoto, Qiong Ding, Tatsuhiro HisatsuneAbstract:Anserine/carnosine supplementation improves cerebral blood flow and verbal episodic memory in elderly people, as we previously reported. Anserine’s buffering activity is superior to that of carnosine at neutral pH. In human sera, carnosine but not Anserine is rapidly cleaved by carnosinase, limiting its effectiveness. This study examined the effects of Anserine on AβPPswe/PSEN1dE9 Alzheimer’s disease (AD) model mice over 18-months old, an age at which these mice exhibit detectable memory deficits. We found that 8 weeks of Anserine treatment completely recovered the memory deficits, improved pericyte coverage on endothelial cells in the brain, and diminished chronic glial neuroinflammatory reactions in these mice. These results suggest that Anserine (beta-alanyl-3-methyl-L-histidine) supplementation improved memory functions in AD-model mice by exerting a protective effect on the neurovascular units, which are composed of endothelial cells, pericytes, and supporting glial cells.
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Anserine (beta-alanyl-3-methyl-L-histidine) improves neurovascular-unit dysfunction and spatial memory in aged AβPPswe/PSEN1dE9 Alzheimer’s-model mice
Nature Publishing Group, 2017Co-Authors: Jun Kaneko, Akiko Enya, Kota Enomoto, Qiong Ding, Tatsuhiro HisatsuneAbstract:Anserine/carnosine supplementation improves cerebral blood flow and verbal episodic memory in elderly people, as we previously reported. Anserine’s buffering activity is superior to that of carnosine at neutral pH. In human sera, carnosine but not Anserine is rapidly cleaved by carnosinase, limiting its effectiveness. This study examined the effects of Anserine on AβPPswe/PSEN1dE9 Alzheimer’s disease (AD) model mice over 18-months old, an age at which these mice exhibit detectable memory deficits. We found that 8 weeks of Anserine treatment completely recovered the memory deficits, improved pericyte coverage on endothelial cells in the brain, and diminished chronic glial neuroinflammatory reactions in these mice. These results suggest that Anserine (beta-alanyl-3-methyl-L-histidine) supplementation improved memory functions in AD-model mice by exerting a protective effect on the neurovascular units, which are composed of endothelial cells, pericytes, and supporting glial cells
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daily carnosine and Anserine supplementation alters verbal episodic memory and resting state network connectivity in healthy elderly adults
Frontiers in Aging Neuroscience, 2015Co-Authors: Jaroslav Rokicki, Jun Kaneko, Tatsuhiro Hisatsune, Etsuko Imabayashi, Hiroshi MatsudaAbstract:Carnosine and Anserine are strong antioxidants, previously demonstrated to reduce cognitive decline in animal studies. We aimed to investigate their cognitive and neurophysiological effects, using functional MRI, on humans. Thirty-one healthy participants (age 40-78, 10~male/21~female) were recruited to a double-blind placebo-controlled study. Participants were assigned to twice-daily doses of imidazole dipeptide formula ($n = 14$), containing 500~mg (carnosine/Anserine, ratio 1/3) or an identical placebo ($n = 17$). Functional MRI and neuropsychological assessments were carried out at baseline and after 3 months of supplementation. We analyzed resting state functional connectivity with the FSL fMRI analysis package. There were no differences in neuropsychological scores between the groups at baseline. After 3 months of supplementation, the carnosine/Anserine group had better verbal episodic memory performance and decreased connectivity in the Default Mode Network, the Posterior Cingulate Cortex and the Right Fronto Parietal Network, as compared with the placebo group. Furthermore, there was a correlation between the extents of cognitive and neuroimaging changes. These results suggest that daily carnosine/Anserine supplementation can impact cognitive function and that network connectivity changes are associated with its effects.
Asunción Cremades - One of the best experts on this subject based on the ideXlab platform.
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Gender-related differences in carnosine, Anserine and lysine content of murine skeletal muscle.
Amino acids, 2003Co-Authors: Rafael Peñafiel, Carolina Ruzafa, F. Monserrat, Asunción CremadesAbstract:The aminoacyl-imidazole dipeptides carnosine (β-alanyl-L-histidine) and Anserine (β-alanyl-1-methyl-histidine) are present in relatively high concentrations in excitable tissues, such as muscle and nervous tissue. In the present study we describe the existence of a marked sexual dimorphism of carnosine and Anserine in skeletal muscles of CD1 mice. In adult animals the concentrations of Anserine were higher than those of carnosine in all skeletal muscles studied, and the content of aminoacyl-imidazole dipeptides was remarkably higher in males than in females. Postnatal ontogenic studies and hormonal manipulations indicated that carnosine synthesis was up-regulated by testosterone whereas Anserine synthesis increased with age. Regional variations in the concentrations of the dipeptides were observed in both sexes, skeletal muscles from hind legs having higher amounts of carnosine and Anserine than those present in fore legs or in the pectoral region. The concentration of L-lysine in skeletal muscles also showed regional variations and a sexual dimorphic pattern with females having higher levels than males in all muscles studied. The results suggest that these differences may be related with the anabolic action of androgens on skeletal muscle.
Jun Kaneko - One of the best experts on this subject based on the ideXlab platform.
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Anserine beta alanyl 3 methyl l histidine improves neurovascular unit dysfunction and spatial memory in aged aβppswe psen1de9 alzheimer s model mice
Scientific Reports, 2017Co-Authors: Jun Kaneko, Akiko Enya, Kota Enomoto, Qiong Ding, Tatsuhiro HisatsuneAbstract:Anserine/carnosine supplementation improves cerebral blood flow and verbal episodic memory in elderly people, as we previously reported. Anserine’s buffering activity is superior to that of carnosine at neutral pH. In human sera, carnosine but not Anserine is rapidly cleaved by carnosinase, limiting its effectiveness. This study examined the effects of Anserine on AβPPswe/PSEN1dE9 Alzheimer’s disease (AD) model mice over 18-months old, an age at which these mice exhibit detectable memory deficits. We found that 8 weeks of Anserine treatment completely recovered the memory deficits, improved pericyte coverage on endothelial cells in the brain, and diminished chronic glial neuroinflammatory reactions in these mice. These results suggest that Anserine (beta-alanyl-3-methyl-L-histidine) supplementation improved memory functions in AD-model mice by exerting a protective effect on the neurovascular units, which are composed of endothelial cells, pericytes, and supporting glial cells.
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Anserine (beta-alanyl-3-methyl-L-histidine) improves neurovascular-unit dysfunction and spatial memory in aged AβPPswe/PSEN1dE9 Alzheimer’s-model mice
Nature Publishing Group, 2017Co-Authors: Jun Kaneko, Akiko Enya, Kota Enomoto, Qiong Ding, Tatsuhiro HisatsuneAbstract:Anserine/carnosine supplementation improves cerebral blood flow and verbal episodic memory in elderly people, as we previously reported. Anserine’s buffering activity is superior to that of carnosine at neutral pH. In human sera, carnosine but not Anserine is rapidly cleaved by carnosinase, limiting its effectiveness. This study examined the effects of Anserine on AβPPswe/PSEN1dE9 Alzheimer’s disease (AD) model mice over 18-months old, an age at which these mice exhibit detectable memory deficits. We found that 8 weeks of Anserine treatment completely recovered the memory deficits, improved pericyte coverage on endothelial cells in the brain, and diminished chronic glial neuroinflammatory reactions in these mice. These results suggest that Anserine (beta-alanyl-3-methyl-L-histidine) supplementation improved memory functions in AD-model mice by exerting a protective effect on the neurovascular units, which are composed of endothelial cells, pericytes, and supporting glial cells
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daily carnosine and Anserine supplementation alters verbal episodic memory and resting state network connectivity in healthy elderly adults
Frontiers in Aging Neuroscience, 2015Co-Authors: Jaroslav Rokicki, Jun Kaneko, Tatsuhiro Hisatsune, Etsuko Imabayashi, Hiroshi MatsudaAbstract:Carnosine and Anserine are strong antioxidants, previously demonstrated to reduce cognitive decline in animal studies. We aimed to investigate their cognitive and neurophysiological effects, using functional MRI, on humans. Thirty-one healthy participants (age 40-78, 10~male/21~female) were recruited to a double-blind placebo-controlled study. Participants were assigned to twice-daily doses of imidazole dipeptide formula ($n = 14$), containing 500~mg (carnosine/Anserine, ratio 1/3) or an identical placebo ($n = 17$). Functional MRI and neuropsychological assessments were carried out at baseline and after 3 months of supplementation. We analyzed resting state functional connectivity with the FSL fMRI analysis package. There were no differences in neuropsychological scores between the groups at baseline. After 3 months of supplementation, the carnosine/Anserine group had better verbal episodic memory performance and decreased connectivity in the Default Mode Network, the Posterior Cingulate Cortex and the Right Fronto Parietal Network, as compared with the placebo group. Furthermore, there was a correlation between the extents of cognitive and neuroimaging changes. These results suggest that daily carnosine/Anserine supplementation can impact cognitive function and that network connectivity changes are associated with its effects.
Rafael Peñafiel - One of the best experts on this subject based on the ideXlab platform.
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Gender-related differences in carnosine, Anserine and lysine content of murine skeletal muscle.
Amino acids, 2003Co-Authors: Rafael Peñafiel, Carolina Ruzafa, F. Monserrat, Asunción CremadesAbstract:The aminoacyl-imidazole dipeptides carnosine (β-alanyl-L-histidine) and Anserine (β-alanyl-1-methyl-histidine) are present in relatively high concentrations in excitable tissues, such as muscle and nervous tissue. In the present study we describe the existence of a marked sexual dimorphism of carnosine and Anserine in skeletal muscles of CD1 mice. In adult animals the concentrations of Anserine were higher than those of carnosine in all skeletal muscles studied, and the content of aminoacyl-imidazole dipeptides was remarkably higher in males than in females. Postnatal ontogenic studies and hormonal manipulations indicated that carnosine synthesis was up-regulated by testosterone whereas Anserine synthesis increased with age. Regional variations in the concentrations of the dipeptides were observed in both sexes, skeletal muscles from hind legs having higher amounts of carnosine and Anserine than those present in fore legs or in the pectoral region. The concentration of L-lysine in skeletal muscles also showed regional variations and a sexual dimorphic pattern with females having higher levels than males in all muscles studied. The results suggest that these differences may be related with the anabolic action of androgens on skeletal muscle.
Tetsuro Yamashita - One of the best experts on this subject based on the ideXlab platform.
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LC-ESI-MS/MS quantification of carnosine, Anserine, and balenine in meat samples
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2019Co-Authors: Reiko Uenoyama, Masao Miyazaki, Tamako Miyazaki, Yuhei Shigeno, Yoshinori Tokairin, Hiroyuki Konno, Tetsuro YamashitaAbstract:Abstract The histidine-containing imidazole dipeptide carnosine and its methylated analogs Anserine and balenine are present at high concentrations in vertebrate tissues. Although the physiological functions of the imidazole dipeptides have not been elucidated yet, it has been suggested that they play significant biological roles in animals. Despite increasing interest, few studies have challenged the quantifications of carnosine, Anserine, and balenine by a single HPLC run because they have similar retention times. In this study, we developed a method to quantify these imidazole dipeptides in meat samples using an LC-ESI-MS/MS triple-quadrupole mass spectrometer. We improved the liquid chromatographic separation of the imidazole dipeptides by applying a mix-mode column, which provides both normal phase and ion exchange separations, and developed multiple reaction-monitoring of the transitions for quantification of m/z 227 → 110 for carnosine, m/z 241 → 126 for Anserine, m/z 241 → 124 for balenine, and m/z 269 → 110 for L-histidyl-L-leucine (internal standard). The established method met all pre-defined validation criteria. Intra- and inter-day accuracy and precision were ±10.0% and ≤14.8%, respectively. The ranges of quantifications were 14.7 ng/mL to 1.5 mg/mL for carnosine, 15.6 ng/mL to 1.6 mg/mL for Anserine, and 15.6 ng/mL to 1.6 mg/mL for balenine. In conclusion, the validated method was successfully applied to the quantification of imidazole dipeptides in biological samples without derivatization.