The Experts below are selected from a list of 2169 Experts worldwide ranked by ideXlab platform

Jens D Mikkelsen - One of the best experts on this subject based on the ideXlab platform.

  • afferent projections to the hamster intergeniculate leaflet demonstrated by retrograde and Anterograde Tracing
    Brain Research Bulletin, 2003
    Co-Authors: Niels Vrang, N Mrosovsky, Jens D Mikkelsen
    Abstract:

    The intergeniculate leaflet (IGL) is considered involved in nonphotic shifting of the circadian clock through a direct connection, the geniculo-hypothalamic tract. The brain areas mediating nonphotic arousal to the hamster IGL have not been thoroughly investigated by both retrograde and Anterograde Tracing. We, therefore, reinvestigated the IGL afferent connections with the retrograde tracer Cholera toxin B and subsequently verified the results with the Anterograde tracer Phaseolus vulgaris-leucoagglutinin. We also defined a subset of neurons projecting to the IGL that were activated by arousal using c-Fos immunocytochemistry. Apart from a dense afferent projection from the retina- and the contralateral leaflet, there were ipsilateral projections from other structures: layer V and VI of the prefrontal cortex, the zona incerta, the magnocellular part of the subparafascicular nucleus, the dorsal raphe nucleus, the locus coeruleus, and the cuneiform nucleus. Dense bilateral projections to the leaflet from the pretectal nuclei were found. Hypothalamic afferents were observed dorsal to the suprachiasmatic nuclei, in the retrochiasmatic area (RCh) and in the ventromedial hypothalamic nuclei. All of these projections were confirmed by Anterograde Tracing. Furthermore, arousal (wheel-running) induced c-Fos in neurons projecting to the IGL (prefrontal cortex, RCh, pretectum). Taken together, the data strengthen the view that the IGL integrates photic and nonphotic information.

  • origin of projections from the midbrain raphe nuclei to the hypothalamic paraventricular nucleus in the rat a combined retrograde and Anterograde Tracing study
    Neuroscience, 1996
    Co-Authors: Philip J Larsen, Anders Hayschmidt, Niels Vrang, Jens D Mikkelsen
    Abstract:

    Abstract A number of neuronal functions governed by the hypothalamic paraventricular nucleus are influenced by serotonin, and it is generally believed that the moderate density of serotonin-immunoreactive fibres and terminals within the paraventricular nucleus originates from the midbrain dorsal and median raphe nuclei. To further evaluate the intricate anatomy of projections from brain stem raphe nuclei of the rat, a combination of retrograde and Anterograde Tracing experiments were conducted to determine the medullary raphe nuclei projection to the paraventricular nucleus. Rhodamine-labelled latex microspheres, Cholera toxin subunit B and FluoroGold we used as retrograde tracers. Intracerebroventricular injections into the third ventricle of all retrograde tracers labelled a distinct population of neurons in the dorsal raphe situated in the subependymal stratum adjacent to the cerebral aqueduct indicating that these cells take up the tracer from the cerebrospinal fluid. Very few retrogradely labelled neurons were seen in the median raphe after i.c.v. administration of the tracers. Retrograde tracers delivered into the medial part of the paraventricular nucleus labelled no further cells in the midbrain dorsal and median raphe nuclei, whereas a substantial number of retrogradely labelled cells emerged in the pontine raphe magnus. However, when the retrograde tracers were delivered into the lateral part of the paraventricular nucleus, avoiding leakage of the tracer into the ventricle, very few labelled neurons were seen in the dorsal and median raphe, whereas the prominent labelling of raphe magnus neurons persisted. The anatomical organization of nerve fibres terminating in the area of paraventricular nucleus originating from midbrain raphe nuclei was studied in a series of Anterograde Tracing experiments using the plant lectin Phaseolus vulgaris leucoagglutinin. Injections delivered into the dorsal raphe or median raphe labelled but a few fibres in the paraventricular nucleus proper. A high number of fine calibered nerve fibres overlying the ependyma adjacent to the paraventricular nucleus was, however, seen after the injections into the subependymal rostral part of the dorsal raphe. Injections delivered into the raphe magnus gave rise to a dense plexus of terminating fibres in the parvicellular parts of the paraventricular nucleus and moderately innervated the posterior magnocellular part of the paraventricular nucleus as well as the magnocellular supraoptic nucleus. Concomitant visualization of serotonin-immunoreactive neurons and retrograde FluoroGold-Tracing from the paraventricular nucleus revealed that none of the serotonergic neurons of the raphe magnus projects to this nucleus, while a few of the neurons putatively projecting to the paraventricular nucleus from the median raphe are serotonergic. The current observations suggest that the raphe magnus constitute by far the largest raphe input to the paraventricular nucleus and strongly questions the earlier held view that most raphe fibres innervating the paraventricular nucleus are derived from the midbrain dorsal and median raphe. However, the source of serotonergic innervation of the paraventricular nucleus remains elusive.

  • origin of projections from the midbrain raphe nuclei to the hypothalamic paraventricular nucleus in the rat a combined retrograde and Anterograde Tracing study
    Neuroscience, 1996
    Co-Authors: Philip J Larsen, Anders Hayschmidt, Niels Vrang, Jens D Mikkelsen
    Abstract:

    A number of neuronal functions governed by the hypothalamic paraventricular nucleus are influenced by serotonin, and it is generally believed that the moderate density of serotonin-immunoreactive fibres and terminals within the paraventricular nucleus originates from the midbrain dorsal and median raphe nuclei. To further evaluate the intricate anatomy of projections from brain stem raphe nuclei of the rat, a combination of retrograde and Anterograde Tracing experiments were conducted to determine the medullary raphe nuclei projection to the paraventricular nucleus. Rhodamine-labelled latex microspheres, Cholera toxin subunit B and FluoroGold we used as retrograde tracers. Intracerebroventricular injections into the third ventricle of all retrograde tracers labelled a distinct population of neurons in the dorsal raphe situated in the subependymal stratum adjacent to the cerebral aqueduct indicating that these cells take up the tracer from the cerebrospinal fluid. Very few retrogradely labelled neurons were seen in the median raphe after i.c.v. administration of the tracers. Retrograde tracers delivered into the medial part of the paraventricular nucleus labelled no further cells in the midbrain dorsal and median raphe nuclei, whereas a substantial number of retrogradely labelled cells emerged in the pontine raphe magnus. However, when the retrograde tracers were delivered into the lateral part of the paraventricular nucleus, avoiding leakage of the tracer into the ventricle, very few labelled neurons were seen in the dorsal and median raphe, whereas the prominent labelling of raphe magnus neurons persisted. The anatomical organization of nerve fibres terminating in the area of the paraventricular nucleus originating from midbrain raphe nuclei was studied in a series of Anterograde Tracing experiments using the plant lectin Phaseolus vulgaris leucoagglutinin. Injections delivered into the dorsal raphe or median raphe labelled but a few fibres in the paraventricular nucleus proper. A high number of fine calibered nerve fibres overlying the ependyma adjacent to the paraventricular nucleus was, however, seen after the injections into the subependymal rostral part of the dorsal raphe. Injections delivered into the raphe magnus gave rise to a dense plexus of terminating fibres in the parvicellular parts of the paraventricular nucleus and moderately innervated the posterior magnocellular part of the paraventricular nucleus as well as the magnocellular supraoptic nucleus. Concomitant visualization of serotonin-immunoreactive neurons and retrograde FluoroGold-Tracing from the paraventricular nucleus revealed that none of the serotonergic neurons of the raphe magnus projects to this nucleus, while a few of the neurons putatively projecting to the paraventricular nucleus from the median raphe are serotonergic. The current observations suggest that the raphe magnus constitute by far the largest raphe input to the paraventricular nucleus and strongly questions the earlier held view that most raphe fibres innervating the paraventricular nucleus are derived from the midbrain dorsal and median raphe. However, the source of serotonergic innervation of the paraventricular nucleus remains elusive.

Philip J Larsen - One of the best experts on this subject based on the ideXlab platform.

  • origin of projections from the midbrain raphe nuclei to the hypothalamic paraventricular nucleus in the rat a combined retrograde and Anterograde Tracing study
    Neuroscience, 1996
    Co-Authors: Philip J Larsen, Anders Hayschmidt, Niels Vrang, Jens D Mikkelsen
    Abstract:

    Abstract A number of neuronal functions governed by the hypothalamic paraventricular nucleus are influenced by serotonin, and it is generally believed that the moderate density of serotonin-immunoreactive fibres and terminals within the paraventricular nucleus originates from the midbrain dorsal and median raphe nuclei. To further evaluate the intricate anatomy of projections from brain stem raphe nuclei of the rat, a combination of retrograde and Anterograde Tracing experiments were conducted to determine the medullary raphe nuclei projection to the paraventricular nucleus. Rhodamine-labelled latex microspheres, Cholera toxin subunit B and FluoroGold we used as retrograde tracers. Intracerebroventricular injections into the third ventricle of all retrograde tracers labelled a distinct population of neurons in the dorsal raphe situated in the subependymal stratum adjacent to the cerebral aqueduct indicating that these cells take up the tracer from the cerebrospinal fluid. Very few retrogradely labelled neurons were seen in the median raphe after i.c.v. administration of the tracers. Retrograde tracers delivered into the medial part of the paraventricular nucleus labelled no further cells in the midbrain dorsal and median raphe nuclei, whereas a substantial number of retrogradely labelled cells emerged in the pontine raphe magnus. However, when the retrograde tracers were delivered into the lateral part of the paraventricular nucleus, avoiding leakage of the tracer into the ventricle, very few labelled neurons were seen in the dorsal and median raphe, whereas the prominent labelling of raphe magnus neurons persisted. The anatomical organization of nerve fibres terminating in the area of paraventricular nucleus originating from midbrain raphe nuclei was studied in a series of Anterograde Tracing experiments using the plant lectin Phaseolus vulgaris leucoagglutinin. Injections delivered into the dorsal raphe or median raphe labelled but a few fibres in the paraventricular nucleus proper. A high number of fine calibered nerve fibres overlying the ependyma adjacent to the paraventricular nucleus was, however, seen after the injections into the subependymal rostral part of the dorsal raphe. Injections delivered into the raphe magnus gave rise to a dense plexus of terminating fibres in the parvicellular parts of the paraventricular nucleus and moderately innervated the posterior magnocellular part of the paraventricular nucleus as well as the magnocellular supraoptic nucleus. Concomitant visualization of serotonin-immunoreactive neurons and retrograde FluoroGold-Tracing from the paraventricular nucleus revealed that none of the serotonergic neurons of the raphe magnus projects to this nucleus, while a few of the neurons putatively projecting to the paraventricular nucleus from the median raphe are serotonergic. The current observations suggest that the raphe magnus constitute by far the largest raphe input to the paraventricular nucleus and strongly questions the earlier held view that most raphe fibres innervating the paraventricular nucleus are derived from the midbrain dorsal and median raphe. However, the source of serotonergic innervation of the paraventricular nucleus remains elusive.

  • origin of projections from the midbrain raphe nuclei to the hypothalamic paraventricular nucleus in the rat a combined retrograde and Anterograde Tracing study
    Neuroscience, 1996
    Co-Authors: Philip J Larsen, Anders Hayschmidt, Niels Vrang, Jens D Mikkelsen
    Abstract:

    A number of neuronal functions governed by the hypothalamic paraventricular nucleus are influenced by serotonin, and it is generally believed that the moderate density of serotonin-immunoreactive fibres and terminals within the paraventricular nucleus originates from the midbrain dorsal and median raphe nuclei. To further evaluate the intricate anatomy of projections from brain stem raphe nuclei of the rat, a combination of retrograde and Anterograde Tracing experiments were conducted to determine the medullary raphe nuclei projection to the paraventricular nucleus. Rhodamine-labelled latex microspheres, Cholera toxin subunit B and FluoroGold we used as retrograde tracers. Intracerebroventricular injections into the third ventricle of all retrograde tracers labelled a distinct population of neurons in the dorsal raphe situated in the subependymal stratum adjacent to the cerebral aqueduct indicating that these cells take up the tracer from the cerebrospinal fluid. Very few retrogradely labelled neurons were seen in the median raphe after i.c.v. administration of the tracers. Retrograde tracers delivered into the medial part of the paraventricular nucleus labelled no further cells in the midbrain dorsal and median raphe nuclei, whereas a substantial number of retrogradely labelled cells emerged in the pontine raphe magnus. However, when the retrograde tracers were delivered into the lateral part of the paraventricular nucleus, avoiding leakage of the tracer into the ventricle, very few labelled neurons were seen in the dorsal and median raphe, whereas the prominent labelling of raphe magnus neurons persisted. The anatomical organization of nerve fibres terminating in the area of the paraventricular nucleus originating from midbrain raphe nuclei was studied in a series of Anterograde Tracing experiments using the plant lectin Phaseolus vulgaris leucoagglutinin. Injections delivered into the dorsal raphe or median raphe labelled but a few fibres in the paraventricular nucleus proper. A high number of fine calibered nerve fibres overlying the ependyma adjacent to the paraventricular nucleus was, however, seen after the injections into the subependymal rostral part of the dorsal raphe. Injections delivered into the raphe magnus gave rise to a dense plexus of terminating fibres in the parvicellular parts of the paraventricular nucleus and moderately innervated the posterior magnocellular part of the paraventricular nucleus as well as the magnocellular supraoptic nucleus. Concomitant visualization of serotonin-immunoreactive neurons and retrograde FluoroGold-Tracing from the paraventricular nucleus revealed that none of the serotonergic neurons of the raphe magnus projects to this nucleus, while a few of the neurons putatively projecting to the paraventricular nucleus from the median raphe are serotonergic. The current observations suggest that the raphe magnus constitute by far the largest raphe input to the paraventricular nucleus and strongly questions the earlier held view that most raphe fibres innervating the paraventricular nucleus are derived from the midbrain dorsal and median raphe. However, the source of serotonergic innervation of the paraventricular nucleus remains elusive.

Niels Vrang - One of the best experts on this subject based on the ideXlab platform.

  • afferent projections to the hamster intergeniculate leaflet demonstrated by retrograde and Anterograde Tracing
    Brain Research Bulletin, 2003
    Co-Authors: Niels Vrang, N Mrosovsky, Jens D Mikkelsen
    Abstract:

    The intergeniculate leaflet (IGL) is considered involved in nonphotic shifting of the circadian clock through a direct connection, the geniculo-hypothalamic tract. The brain areas mediating nonphotic arousal to the hamster IGL have not been thoroughly investigated by both retrograde and Anterograde Tracing. We, therefore, reinvestigated the IGL afferent connections with the retrograde tracer Cholera toxin B and subsequently verified the results with the Anterograde tracer Phaseolus vulgaris-leucoagglutinin. We also defined a subset of neurons projecting to the IGL that were activated by arousal using c-Fos immunocytochemistry. Apart from a dense afferent projection from the retina- and the contralateral leaflet, there were ipsilateral projections from other structures: layer V and VI of the prefrontal cortex, the zona incerta, the magnocellular part of the subparafascicular nucleus, the dorsal raphe nucleus, the locus coeruleus, and the cuneiform nucleus. Dense bilateral projections to the leaflet from the pretectal nuclei were found. Hypothalamic afferents were observed dorsal to the suprachiasmatic nuclei, in the retrochiasmatic area (RCh) and in the ventromedial hypothalamic nuclei. All of these projections were confirmed by Anterograde Tracing. Furthermore, arousal (wheel-running) induced c-Fos in neurons projecting to the IGL (prefrontal cortex, RCh, pretectum). Taken together, the data strengthen the view that the IGL integrates photic and nonphotic information.

  • origin of projections from the midbrain raphe nuclei to the hypothalamic paraventricular nucleus in the rat a combined retrograde and Anterograde Tracing study
    Neuroscience, 1996
    Co-Authors: Philip J Larsen, Anders Hayschmidt, Niels Vrang, Jens D Mikkelsen
    Abstract:

    Abstract A number of neuronal functions governed by the hypothalamic paraventricular nucleus are influenced by serotonin, and it is generally believed that the moderate density of serotonin-immunoreactive fibres and terminals within the paraventricular nucleus originates from the midbrain dorsal and median raphe nuclei. To further evaluate the intricate anatomy of projections from brain stem raphe nuclei of the rat, a combination of retrograde and Anterograde Tracing experiments were conducted to determine the medullary raphe nuclei projection to the paraventricular nucleus. Rhodamine-labelled latex microspheres, Cholera toxin subunit B and FluoroGold we used as retrograde tracers. Intracerebroventricular injections into the third ventricle of all retrograde tracers labelled a distinct population of neurons in the dorsal raphe situated in the subependymal stratum adjacent to the cerebral aqueduct indicating that these cells take up the tracer from the cerebrospinal fluid. Very few retrogradely labelled neurons were seen in the median raphe after i.c.v. administration of the tracers. Retrograde tracers delivered into the medial part of the paraventricular nucleus labelled no further cells in the midbrain dorsal and median raphe nuclei, whereas a substantial number of retrogradely labelled cells emerged in the pontine raphe magnus. However, when the retrograde tracers were delivered into the lateral part of the paraventricular nucleus, avoiding leakage of the tracer into the ventricle, very few labelled neurons were seen in the dorsal and median raphe, whereas the prominent labelling of raphe magnus neurons persisted. The anatomical organization of nerve fibres terminating in the area of paraventricular nucleus originating from midbrain raphe nuclei was studied in a series of Anterograde Tracing experiments using the plant lectin Phaseolus vulgaris leucoagglutinin. Injections delivered into the dorsal raphe or median raphe labelled but a few fibres in the paraventricular nucleus proper. A high number of fine calibered nerve fibres overlying the ependyma adjacent to the paraventricular nucleus was, however, seen after the injections into the subependymal rostral part of the dorsal raphe. Injections delivered into the raphe magnus gave rise to a dense plexus of terminating fibres in the parvicellular parts of the paraventricular nucleus and moderately innervated the posterior magnocellular part of the paraventricular nucleus as well as the magnocellular supraoptic nucleus. Concomitant visualization of serotonin-immunoreactive neurons and retrograde FluoroGold-Tracing from the paraventricular nucleus revealed that none of the serotonergic neurons of the raphe magnus projects to this nucleus, while a few of the neurons putatively projecting to the paraventricular nucleus from the median raphe are serotonergic. The current observations suggest that the raphe magnus constitute by far the largest raphe input to the paraventricular nucleus and strongly questions the earlier held view that most raphe fibres innervating the paraventricular nucleus are derived from the midbrain dorsal and median raphe. However, the source of serotonergic innervation of the paraventricular nucleus remains elusive.

  • origin of projections from the midbrain raphe nuclei to the hypothalamic paraventricular nucleus in the rat a combined retrograde and Anterograde Tracing study
    Neuroscience, 1996
    Co-Authors: Philip J Larsen, Anders Hayschmidt, Niels Vrang, Jens D Mikkelsen
    Abstract:

    A number of neuronal functions governed by the hypothalamic paraventricular nucleus are influenced by serotonin, and it is generally believed that the moderate density of serotonin-immunoreactive fibres and terminals within the paraventricular nucleus originates from the midbrain dorsal and median raphe nuclei. To further evaluate the intricate anatomy of projections from brain stem raphe nuclei of the rat, a combination of retrograde and Anterograde Tracing experiments were conducted to determine the medullary raphe nuclei projection to the paraventricular nucleus. Rhodamine-labelled latex microspheres, Cholera toxin subunit B and FluoroGold we used as retrograde tracers. Intracerebroventricular injections into the third ventricle of all retrograde tracers labelled a distinct population of neurons in the dorsal raphe situated in the subependymal stratum adjacent to the cerebral aqueduct indicating that these cells take up the tracer from the cerebrospinal fluid. Very few retrogradely labelled neurons were seen in the median raphe after i.c.v. administration of the tracers. Retrograde tracers delivered into the medial part of the paraventricular nucleus labelled no further cells in the midbrain dorsal and median raphe nuclei, whereas a substantial number of retrogradely labelled cells emerged in the pontine raphe magnus. However, when the retrograde tracers were delivered into the lateral part of the paraventricular nucleus, avoiding leakage of the tracer into the ventricle, very few labelled neurons were seen in the dorsal and median raphe, whereas the prominent labelling of raphe magnus neurons persisted. The anatomical organization of nerve fibres terminating in the area of the paraventricular nucleus originating from midbrain raphe nuclei was studied in a series of Anterograde Tracing experiments using the plant lectin Phaseolus vulgaris leucoagglutinin. Injections delivered into the dorsal raphe or median raphe labelled but a few fibres in the paraventricular nucleus proper. A high number of fine calibered nerve fibres overlying the ependyma adjacent to the paraventricular nucleus was, however, seen after the injections into the subependymal rostral part of the dorsal raphe. Injections delivered into the raphe magnus gave rise to a dense plexus of terminating fibres in the parvicellular parts of the paraventricular nucleus and moderately innervated the posterior magnocellular part of the paraventricular nucleus as well as the magnocellular supraoptic nucleus. Concomitant visualization of serotonin-immunoreactive neurons and retrograde FluoroGold-Tracing from the paraventricular nucleus revealed that none of the serotonergic neurons of the raphe magnus projects to this nucleus, while a few of the neurons putatively projecting to the paraventricular nucleus from the median raphe are serotonergic. The current observations suggest that the raphe magnus constitute by far the largest raphe input to the paraventricular nucleus and strongly questions the earlier held view that most raphe fibres innervating the paraventricular nucleus are derived from the midbrain dorsal and median raphe. However, the source of serotonergic innervation of the paraventricular nucleus remains elusive.

Gary Astonjones - One of the best experts on this subject based on the ideXlab platform.

  • extensive projections from the midbrain periaqueductal gray to the caudal ventrolateral medulla a retrograde and Anterograde Tracing study in the rat
    Neuroscience, 1996
    Co-Authors: S Chen, Gary Astonjones
    Abstract:

    We investigated the innervation of the caudal ventrolateral medulla by the midbrain periaqueductal gray in the rat using retrograde and Anterograde tract-Tracing. Iontophoretic injection of Fluoro-Gold or cholera toxin B subunit into the caudal ventrolateral medulla resulted in retrogradely labeled neurons in discrete regions of the periaqueductal gray. These labeled cells were observed throughout the rostrocaudal extent of the periaqueductal gray and were distributed (as percentage of total labeled cells) in its lateral (53-67%), ventrolateral (14-28%), ventromedial (7-16%) and dorsomedial aspects (7-10%). About 70-72% of labeled cells were found in the caudal half of the periaqueductal gray and 28-30% in the rostral half. In the ventromedial periaqueductal gray, more labeled cells were seen in the contralateral side (5-13%) than the ipsilateral side (2-3%), whereas for other periaqueductal gray areas labeling was preferentially ipsilateral. Phaseolus vulgaris leucoagglutinin Anterograde Tracing was used to confirm the retrograde labeling results. Following iontophoretic injection into the periaqueductal gray, labeled fibers and terminals were observed throughout the rostrocaudal extent of the caudal ventrolateral medulla. Injections in the lateral and/or ventrolateral aspect of the periaqueductal gray yielded more Anterograde labeling in the ipsilateral than the contralateral caudal ventrolateral medulla, while injections in the ventromedial aspect of the periaqueductal gray produced labeling preferentially in the contralateral caudal ventrolateral medulla. The present study indicates that specific regions of the periaqueductal gray project to the caudal ventrolateral medulla and may regulate cardiovascular and respiratory functions through these connections.

  • anatomical evidence for inputs to ventrolateral medullary catecholaminergic neurons from the midbrain periaqueductal gray of the rat
    Neuroscience Letters, 1995
    Co-Authors: Sheng Chen, Gary Astonjones
    Abstract:

    Abstract Previous studies have shown that the midbrain periaqueductal gray (PAG) projects to the ventrolateral medulla (VLM). Here, we studied PAG projections to the area of A1/C1 neurons in the VLM in the rat using phaseolus vulgaris leucoagglutinin (PHA-L) Anterograde Tracing combined with immunocytochemistry for tyrosine hydroxylase (TH) or phenylethanolamine N -methyl transferase (PNMT). Following PAG injections, PHA-L labeled fibers and terminals were intermingled among TH-immunoreactive (TH-ir) neurons in the VLM. High-power light microscopic examination revealed that some of the PHA-L labeled varicose fibers and boutons were in close contiguity with TH-ir elements. Such apparent appositions appeared more frequently on TH-ir elements in the A1 area than on TH-ir or PNMT-ir neurons ni the C1 area. These results indicate that some PAG inputs to the VLM may directly innervate A1/C1 neurons

  • afferent projections to the rat locus coeruleus demonstrated by retrograde and Anterograde Tracing with cholera toxin b subunit and phaseolus vulgaris leucoagglutinin
    Neuroscience, 1995
    Co-Authors: Pierre-hervé Luppi, Gary Astonjones, Hideo Akaoka, Guy Chouvet, M Jouvet
    Abstract:

    Abstract The aim of this study was to examine the afferents to the rat locus coeruleus by means of retrograde and Anterograde Tracing experiments using cholera-toxin B subunit and phaseolus leucoagglutinin. To obtain reliable injections of cholera-toxin B in the locus coeruleus, electrophysiological recordings were made through glass micropipettes containing the tracer and the noradrenergic neurons of the locus coeruleus were identified by their characteristic discharge properties. After iontophoretic injections of cholera-toxin B into the nuclear core of the locus coeruleus, we observed a substantial number of retrogradely labeled cells in the lateral paragigantocellular nucleus and the dorsomedial rostral medulla (ventromedial prepositus hypoglossi and dorsal paragigantocellular nuclei) as previously described. 6 We also saw a substantial number of retrogradely labeled neurons in (1) the preoptic area dorsal to the supraoptic nucleus, (2) areas of the posterior hypothalamus, (3) the Kolliker-Fuse nucleus, (4) mesencephalic reticular formation. Fewer labeled cells were also observed in other regions including the hypothalamic paraventricular nucleus, dorsal raphe nucleus, median raphe nucleus, dorsal part of the periaqueductal gray, the area of the noradrenergic A5 group, the lateral parabrachial nucleus and the caudoventrolateral reticular nucleus. No or only occasional cells were found in the cortex, the central nucleus of the amygdala, the lateral part of the bed nucleus of the stria terminalis, the vestibular nuclei, the nucleus of the solitary tract or the spinal cord, structures which were previously reported as inputs to the locus coeruleus. 10,13 Control injections of cholera-toxin B were made in areas surrounding the locus coeruleus, including (1) Barrington's nucleus, (2) the mesencephalic trigeminal nucleus, (3) a previously undefined area immediately rostral to the locus coeruleus and medial to the mesencephalic trigeminal nucleus that we named the peri-mesencephalic trigeminal nucleus, and (4) the medial vestibular nucleus lateral to the caudal tip of the locus coeruleus. These injections yielded patterns of retrograde labeling that differed from one another and also from that obtained with cholera-toxin B injection sites in the locus coeruleus. These results indicate that the area surrounding the locus coeruleus is divided into individual nuclei with distinct afferents. These results were confirmed and extended with Anterograde transport of cholera-toxin B or phaseolus leucoagglutinin. Injections of these tracers in the lateral paragigantocellular nucleus, preoptic area dorsal to the supraoptic nucleus, the ventrolateral part of the periaqueductal gray, the Kolliker-Fuse nucleus yielded a substantial to large number of labeled fibers in the nuclear core of the locus coeruleus. Anterograde transport of cholera-toxin B or phaseolus leucoagglutinin from the posterior hypothalamic areas yielded a moderate to small number of labeled fibers in the nuclear core of the locus coeruleus. These Anterograde Tracing experiments confirm that these areas send direct projections to the rat locus coeruleus. Importantly, fiber labeling from each of these areas was in most cases much denser in areas immediately surrounding the locus coeruleus than in the locus coeruleus proper. In particular, the lamina and the periaqueductal gray medial to the locus coeruleus where many dendrites of locus coeruleus noradrenergic cells are located contained a large number of fibers. These data might indicate that a large number of the afferents to the noradrenergic neurons of the locus coeruleus terminate on dendrites outside the dense core of the nucleus. Further electrophysiological as well as ultrastructural studies are necessary to test this hypothesis.

M Jouvet - One of the best experts on this subject based on the ideXlab platform.

  • afferent projections to the rat locus coeruleus demonstrated by retrograde and Anterograde Tracing with cholera toxin b subunit and phaseolus vulgaris leucoagglutinin
    Neuroscience, 1995
    Co-Authors: Pierre-hervé Luppi, Gary Astonjones, Hideo Akaoka, Guy Chouvet, M Jouvet
    Abstract:

    Abstract The aim of this study was to examine the afferents to the rat locus coeruleus by means of retrograde and Anterograde Tracing experiments using cholera-toxin B subunit and phaseolus leucoagglutinin. To obtain reliable injections of cholera-toxin B in the locus coeruleus, electrophysiological recordings were made through glass micropipettes containing the tracer and the noradrenergic neurons of the locus coeruleus were identified by their characteristic discharge properties. After iontophoretic injections of cholera-toxin B into the nuclear core of the locus coeruleus, we observed a substantial number of retrogradely labeled cells in the lateral paragigantocellular nucleus and the dorsomedial rostral medulla (ventromedial prepositus hypoglossi and dorsal paragigantocellular nuclei) as previously described. 6 We also saw a substantial number of retrogradely labeled neurons in (1) the preoptic area dorsal to the supraoptic nucleus, (2) areas of the posterior hypothalamus, (3) the Kolliker-Fuse nucleus, (4) mesencephalic reticular formation. Fewer labeled cells were also observed in other regions including the hypothalamic paraventricular nucleus, dorsal raphe nucleus, median raphe nucleus, dorsal part of the periaqueductal gray, the area of the noradrenergic A5 group, the lateral parabrachial nucleus and the caudoventrolateral reticular nucleus. No or only occasional cells were found in the cortex, the central nucleus of the amygdala, the lateral part of the bed nucleus of the stria terminalis, the vestibular nuclei, the nucleus of the solitary tract or the spinal cord, structures which were previously reported as inputs to the locus coeruleus. 10,13 Control injections of cholera-toxin B were made in areas surrounding the locus coeruleus, including (1) Barrington's nucleus, (2) the mesencephalic trigeminal nucleus, (3) a previously undefined area immediately rostral to the locus coeruleus and medial to the mesencephalic trigeminal nucleus that we named the peri-mesencephalic trigeminal nucleus, and (4) the medial vestibular nucleus lateral to the caudal tip of the locus coeruleus. These injections yielded patterns of retrograde labeling that differed from one another and also from that obtained with cholera-toxin B injection sites in the locus coeruleus. These results indicate that the area surrounding the locus coeruleus is divided into individual nuclei with distinct afferents. These results were confirmed and extended with Anterograde transport of cholera-toxin B or phaseolus leucoagglutinin. Injections of these tracers in the lateral paragigantocellular nucleus, preoptic area dorsal to the supraoptic nucleus, the ventrolateral part of the periaqueductal gray, the Kolliker-Fuse nucleus yielded a substantial to large number of labeled fibers in the nuclear core of the locus coeruleus. Anterograde transport of cholera-toxin B or phaseolus leucoagglutinin from the posterior hypothalamic areas yielded a moderate to small number of labeled fibers in the nuclear core of the locus coeruleus. These Anterograde Tracing experiments confirm that these areas send direct projections to the rat locus coeruleus. Importantly, fiber labeling from each of these areas was in most cases much denser in areas immediately surrounding the locus coeruleus than in the locus coeruleus proper. In particular, the lamina and the periaqueductal gray medial to the locus coeruleus where many dendrites of locus coeruleus noradrenergic cells are located contained a large number of fibers. These data might indicate that a large number of the afferents to the noradrenergic neurons of the locus coeruleus terminate on dendrites outside the dense core of the nucleus. Further electrophysiological as well as ultrastructural studies are necessary to test this hypothesis.

  • Afferent projections to the rat locus coeruleus demonstrated by retrograde and Anterograde Tracing with cholera-toxin B subunit and Phaseolus vulgaris leucoagglutinin
    Neuroscience, 1995
    Co-Authors: P.-h. Luppi, Hideo Akaoka, Guy Chouvet, G. Aston-jones, M Jouvet
    Abstract:

    The aim of this study was to examine the afferents to the rat locus coeruleus by means of retrograde and Anterograde Tracing experiments using cholera-toxin B subunit and phaseolus leucoagglutinin. To obtain reliable injections of cholera-toxin B in the locus coeruleus, electrophysiological recordings were made through glass micropipettes containing the tracer and the noradrenergic neurons of the locus coeruleus were identified by their characteristic discharge properties. After iontophoretic injections of cholera-toxin B into the nuclear core of the locus coeruleus, we observed a substantial number of retrogradely labeled cells in the lateral paragigantocellular nucleus and the dorsomedial rostral medulla (ventromedial prepositus hypoglossi and dorsal paragigantocellular nuclei) as previously described. We also saw a substantial number of retrogradely labeled neurons in (1) the preoptic area dorsal to the supraoptic nucleus, (2) areas of the posterior hypothalamus, (3) the Kölliker-Fuse nucleus, (4) mesencephalic reticular formation. Fewer labeled cells were also observed in other regions including the hypothalamic paraventricular nucleus, dorsal raphe nucleus, median raphe nucleus, dorsal part of the periaqueductal gray, the area of the noradrenergic A5 group, the lateral parabrachial nucleus and the caudoventrolateral reticular nucleus. No or only occasional cells were found in the cortex, the central nucleus of the amygdala, the lateral part of the bed nucleus of the stria terminalis, the vestibular nuclei, the nucleus of the solitary tract or the spinal cord, structures which were previously reported as inputs to the locus coeruleus. Control injections of cholera-toxin B were made in areas surrounding the locus coeruleus, including (1) Barrington's nucleus, (2) the mesencephalic trigeminal nucleus, (3) a previously undefined area immediately rostral to the locus coeruleus and medial to the mesencephalic trigeminal nucleus that we named the peri-mesencephalic trigeminal nucleus, and (4) the medial vestibular nucleus lateral to the caudal tip of the locus coeruleus. These injections yielded patterns of retrograde labeling that differed from one another and also from that obtained with cholera-toxin B injection sites in the locus coeruleus. These results indicate that the area surrounding the locus coeruleus is divided into individual nuclei with distinct afferents. These results were confirmed and extended with Anterograde transport of cholera-toxin B or phaseolus leucoagglutinin. Injections of these tracers in the lateral paragigantocellular nucleus, preoptic area dorsal to the supraoptic nucleus, the ventrolateral part of the periaqueductal gray, the Kölliker-Fuse nucleus yielded a substantial to large number of labeled fibers in the nuclear core of the locus coeruleus.(ABSTRACT TRUNCATED AT 400 WORDS)