The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Andrea Pedrosa-harand - One of the best experts on this subject based on the ideXlab platform.
-
Breaks of macrosynteny and collinearity among moth bean (Vigna aconitifolia), cowpea (V. unguiculata), and common bean (Phaseolus vulgaris)
Chromosome Research, 2020Co-Authors: Ana Rafaela Da S. Oliveira, Andrea Pedrosa-harand, Lívia Do Vale Martins, Fernanda De O. Bustamante, María Muñoz-amatriaín, Timothy Close, Antônio F. Costa, Ana Maria Benko-iseppon, Ana Christina Brasileiro-vidalAbstract:Comparative cytogenetic mapping is a powerful approach to gain insights into genome organization of orphan crops, lacking a whole sequenced genome. To investigate the cytogenomic evolution of important Vigna and Phaseolus beans, we built a BAC-FISH (fluorescent in situ hybridization of bacterial artificial chromosome) map of Vigna aconitifolia ( Vac , subgenus Ceratotropis ), species with no sequenced genome, and compared with V. unguiculata ( Vu , subgenus Vigna ) and Phaseolus vulgaris ( Pv ) maps. Seventeen Pv BACs, eight Vu BACs, and 5S and 35S rDNA probes were hybridized in situ on the 11 Vac chromosome pairs. Five Vac chromosomes ( Vac 6, Vac 7, Vac 9, Vac 10, and Vac 11) showed conserved macrosynteny and collinearity between V. unguiculata and P. vulgaris . On the other hand, we observed collinearity breaks, identified by pericentric inversions involving Vac 2 ( Vu 2), Vac 4 ( Vu 4), and Vac 3 ( Pv 3). We also detected macrosynteny breaks of translocation type involving chromosomes 1 and 8 of V. aconitifolia and P. vulgaris ; 2 and 3 of V. aconitifolia and P. vulgaris ; and 1 and 5 of V. aconitifolia and V. unguiculata . Considering our data and previous BAC-FISH studies, six chromosomes (1, 2, 3, 4, 5, and 8) are involved in major karyotype divergences between genera and five (1, 2, 3, 4, and 5) between Vigna subgenera, including mechanisms such as duplications, inversions, and translocations. Macrosynteny breaks between Vigna and Phaseolus suggest that the major chromosomal rearrangements have occurred within the Vigna clade. Our cytogenomic comparisons bring new light on the degree of shared macrosynteny and mechanisms of karyotype diversification during Vigna and Phaseolus evolution.
-
Cytogenetics and Comparative Analysis of Phaseolus Species
The Common Bean Genome, 2017Co-Authors: Artur Fonsêca, Andrea Pedrosa-harandAbstract:The genus Phaseolus includes approximately 75 species, most of which have 2n = 22 small meta- or submetacentric chromosomes. This considerable karyotypic stability has been further reinforced by comparative cytogenetic mapping of single-copy sequences using Bacterial Artificial Chromosomes (BACs) and the Fluorescent in situ Hybridization (FISH) technique. These physical maps have revealed complete macrosynteny among the investigated species and only a few breaks in collinearity due to chromosomal inversions, except for Phaseolus leptostachyus, with 2n = 20 and several rearrangements. The variation in the repetitive fraction of the genome is much greater, however, as expected. Considerable variation has been found in the number of 35S rDNA sites in the representatives of the Vulgaris group, and repetitive pericentromeric and subtelomeric sequences vary among Phaseolus species. This repetitive genome fraction has also been investigated in Phaseolus vulgaris through an epigenetic approach, revealing a higher degree of complexity in the heterochromatin than previously thought. The available molecular tools for the common bean have permitted a systematic analysis of the synteny, not only between the common bean and other cultivated and wild species of the genus, but also at the intergeneric level, contributing to a more detailed understanding of the cytogenetics of the group.
-
Evolutionary dynamics of satellite DNA repeats from Phaseolus beans
Protoplasma, 2016Co-Authors: Tiago Ribeiro, Karla G. B. Dos Santos, Manon M. S. Richard, Mireille Sévignac, Vincent Thareau, Valérie Geffroy, Andrea Pedrosa-harandAbstract:Common bean (Phaseolus vulgaris) subtelomeres are highly enriched for khipu, the main satellite DNA identified so far in this genome. Here, we comparatively investigate khipu genomic organization in Phaseolus species from different clades. Additionally, we identified and characterized another satellite repeat, named jumper, associated to khipu. A mixture of P. vulgaris khipu clones hybridized in situ confirmed the presence of khipu-like sequences on subterminal chromosome regions in all Phaseolus species, with differences in the number and intensity of signals between species and when species-specific clones were used. Khipu is present as multimers of ∼500 bp and sequence analyses of cloned fragments revealed close relationship among khipu repeats. The new repeat, named jumper, is a 170-bp satellite sequence present in all Phaseolus species and inserted into the nontranscribed spacer (NTS) of the 5S rDNA in the P. vulgaris genome. Nevertheless, jumper was found as a high-copy repeat at subtelomeres and/or pericentromeres in the Phaseolus microcarpus lineage only. Our data argue for khipu as an important subtelomeric satellite DNA in the genus and for a complex satellite repeat composition of P. microcarpus subtelomeres, which also contain jumper. Furthermore, the differential amplification of these repeats in subtelomeres or pericentromeres reinforces the presence of a dynamic satellite DNA library in Phaseolus.
-
Karyotype stability in the genus Phaseolus evidenced by the comparative mapping of the wild species Phaseolus microcarpus.
Genome, 2013Co-Authors: Artur Fonsêca, Andrea Pedrosa-harandAbstract:The genus Phaseolus L. (Fabaceae) is monophyletic and comprises approximately 75 species distributed into two principal clades. The five cultivated species, including the common bean (Phaseolus vulgaris), were placed in clade B. Clade A comprises only wild species, with more limited distribution. In the present work, bacterial artificial chromosomes (BACs) previously mapped in common bean (2n = 22) were used as probes in fluorescent in situ hybridization (FISH) in this comparative study of Phaseolus microcarpus (2n = 22), a species from clade A. We also analyzed the chromomycin A3 (CMA)/4′,6-diamidino-2-phenylindole (DAPI) banding pattern and the localization of rDNA and telomeric DNA sites. The single 45S rDNA site from P. microcarpus was mapped to chromosome 6, showing conservation to the P. vulgaris homeolog. Of the two 5S rDNA sites identified in both species, only the site on chromosome 10 appeared conserved. In spite of the phylogenetic distance between the two species, all of the single-copy BACs d...
Tanja Mimmo - One of the best experts on this subject based on the ideXlab platform.
-
aluminium phosphate interactions in the rhizosphere of two bean species Phaseolus lunatus l and Phaseolus vulgaris l
Journal of the Science of Food and Agriculture, 2013Co-Authors: Tanja Mimmo, Massimiliano Ghizzi, Stefano Cesco, Nicola Tomasi, R Pinton, Markus PuschenreiterAbstract:BACKGROUND Plants differ in their response to high aluminium (Al) concentrations, which typically cause toxicity in plants grown on acidic soils. The response depends on plant species and environmental conditions such as substrate and cultivation system. The present study aimed to assess Al–phosphate (P) dynamics in the rhizosphere of two bean species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L., in rhizobox experiments. RESULTS Root activity of the bean species induced up to a sevenfold increase in exchangeable Al and up to a 30-fold decrease in extractable P. High soluble Al concentrations triggered the release of plant-specific carboxylates, which differed between soil type and plant species. The results suggest that P. vulgaris L. mitigates Al stress by an internal defence mechanism and P. lunatus L. by an external one, both mechanisms involving organic acids. CONCLUSION Rhizosphere mechanisms involved in Al detoxification were found to be different for P. vulgaris L. and P. lunatus L., suggesting that these processes are plant species-specific. Phaseolus vulgaris L. accumulates Al in the shoots (internal tolerance mechanism), while P. lunatus L. prevents Al uptake by releasing organic acids (exclusion mechanism) into the growth media. © 2013 Society of Chemical Industry
-
Aluminium–phosphate interactions in the rhizosphere of two bean species: Phaseolus lunatus L. and Phaseolus vulgaris L
Journal of the Science of Food and Agriculture, 2013Co-Authors: Tanja Mimmo, Massimiliano Ghizzi, Stefano Cesco, Nicola Tomasi, R Pinton, Markus PuschenreiterAbstract:BACKGROUND Plants differ in their response to high aluminium (Al) concentrations, which typically cause toxicity in plants grown on acidic soils. The response depends on plant species and environmental conditions such as substrate and cultivation system. The present study aimed to assess Al–phosphate (P) dynamics in the rhizosphere of two bean species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L., in rhizobox experiments. RESULTS Root activity of the bean species induced up to a sevenfold increase in exchangeable Al and up to a 30-fold decrease in extractable P. High soluble Al concentrations triggered the release of plant-specific carboxylates, which differed between soil type and plant species. The results suggest that P. vulgaris L. mitigates Al stress by an internal defence mechanism and P. lunatus L. by an external one, both mechanisms involving organic acids. CONCLUSION Rhizosphere mechanisms involved in Al detoxification were found to be different for P. vulgaris L. and P. lunatus L., suggesting that these processes are plant species-specific. Phaseolus vulgaris L. accumulates Al in the shoots (internal tolerance mechanism), while P. lunatus L. prevents Al uptake by releasing organic acids (exclusion mechanism) into the growth media. © 2013 Society of Chemical Industry
-
the influence of aluminium availability on phosphate uptake in Phaseolus vulgaris l and Phaseolus lunatus l
Plant Physiology and Biochemistry, 2009Co-Authors: Tanja Mimmo, Marco Sciortino, Massimiliano Ghizzi, Giorgio Gianquinto, C GessaAbstract:Abstract Aluminium toxicity is one of the major limiting factors of crop productivity on acid soils. High levels of available aluminium in soil may induce phosphorus deficiency in plants. This study investigates the influence of Aluminium (Al) on the phosphate (P i ) uptake of two Phaseolus species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L. The two bean species were treated first with solutions of Al at different concentrations (0, 25, 50 and 100 μM, pH 4.50) and second with solutions of P i (150 μM) at pH 4.50. The higher the Al concentration the higher the Al concentration sorbed but P. vulgaris L var. Red Kidney adsorbed significantly more Al than P. lunatus L. Both species released organic acids: P. vulgaris L var. Red Kidney released fumaric acid and P. lunatus L. fumaric and oxalic acids which could have hindered further Al uptake. The two bean species showed a sigmoid P i uptake trend but with two different mechanisms. P. vulgaris L var. Red Kidney showed a starting point of 3 h whereas P. lunatus L. adsorbed P i immediately within the first minutes. In addition, P. vulgaris L var. Red Kidney presented significantly higher P i uptake (higher uptake rate ‘ k ’ and higher maximum adsorption ‘ a ’ of the kinetic uptake model). The Al treatments did not significantly influence P i uptake. Results suggest that P. lunatus L. might adopt an external Al detoxification mechanism by the release of oxalic acid. P. vulgaris L var. Red Kidney on the other hand seemed to adopt an internal detoxification mechanism even if the Al sorbed is poorly translocated into the shoots. More detailed studies will be necessary to better define Al tolerance and/or resistance of Phaseolus spp.
Jean-pierre Baudoin - One of the best experts on this subject based on the ideXlab platform.
-
Genes involved in Phaseolus embryogenesis
2020Co-Authors: Souleymane Silué, Jean-marie Jacquemin, Jean-pierre BaudoinAbstract:Within the genus Phaseolus, the common bean, Phaseolus vulgaris L., is the most important species widely distributed in the world and occupies more than 90% of production areas sown to Phaseolus species. Interspecific hybridization in the genus Phaseolus, with the aim to introgress desired traits to the common bean, leads to the abortion of immature embryos usually at the globular or early heart-shaped developmental stages. Plant zygotic embryogenesis is controlled by many genes and malfunction of these genes can disrupt embryo formation. In this paper, we reviewed some of these genes i.e. KNOX, BELL1, LEUCINE ZIPPER, PHD-FINGER, GLABRA2, WUSHEL, HEAT SHOCK PROTEIN, LIPID TRANSFER PROTEIN, PASTICCINO, LEAFY COTYLEDON and TITAN, from model plants such as Arabidopsis thaliana, Zea mays, Oryza sativa, Medicago truncatula, Solanum lycopersicum. This study helps us to identify the genes involved in Phaseolus embryogenesis and to verify their expression in ovules at different steps of embryos development.
-
Lima bean: Phaseolus lunatus L.
Genetic Improvement of Vegetable Crops, 2012Co-Authors: Jean-pierre BaudoinAbstract:Publisher Summary The food legume Phaseolus lunatus L.—commonly called Lima bean—belongs to the genus Phaseolus, the subtribe Phaseolinae, the tribe Phaseoleae, the family Papilionaceae or Fabaceae, and the order of Leguminosales or Fabales. Phaseolus lunatus has a neotropical origin with at least two major centers of diversity as suggested by the electrophoretic analysis of crude seed protein: (1) the Central American center (mainly Mexico and Guatemala) for the small-seeded forms and (2) the Andean center (mainly Peru and Ecuador) for the large-seeded forms. Two varieties have been botanically identified within the species: (1) var. Silvester (the wild forms) and (2) var. lunatus (the cultivated forms). The latter is subdivided into three major cultigroups: (1) cv-gr. Sieva with medium-sized, flat seeds, (2) cv-gr. Potato with small, globular seeds, and (3) cv-gr. Big Lima with large, flat seeds. This classification, however, is far from reflecting the whole range of seed diversity among the cultivated materials due to free gene flow occurring between the three cultigroups. The Lima bean is a herbaceous species, including annual determinate bush types and indeterminate climbing types. This chapter discusses the cytology, genetics, germplasm resources, reproductive biology, breeding objectives, and breeding methods of Lima bean.
-
Phaseolus Immature Embryo Rescue Technology
Methods of Molecular Biology, 2010Co-Authors: P. Geerts, André Toussaint, Guy Mergeai, Jean-pierre BaudoinAbstract:: Predominant among the production constraints of the common bean Phaseolus vulgaris are infestation of Ascochyta blight, Bean Golden Mosaic virus (BGMV), and Bean Fly. Interbreeding with Phaseolus -coccineus L. and/or Phaseolus polyanthus Greenm has been shown to provide P. vulgaris with greater resistance to these diseases. For interspecific crosses to be successful, it is important to use P. coccineus and P. polyanthus as female parents; this prevents rapid reversal to the recurrent parent P. vulgaris. Although incompatibility barriers are post-zygotic, early hybrid embryo abortion limits the success of F1 crosses. While rescue techniques for globular and early heart-shaped embryos have improved in recent years, -success in hybridization remains very low. In this study, we describe six steps that allowed us to rescue 2-day-old P. vulgaris embryos using a pod culture technique. Our methods consisted of (i) pod culture, (ii) extraction and culture of immature embryos, (iii) dehydration of embryos, (iv) germination of embryos, (v) rooting of developed shoots, and (vi) hardening of plantlets.
-
Protoplast fusion technology for somatic hybridisation in Phaseolus
Biotechnologie Agronomie Société et Environnement, 2008Co-Authors: P. Geerts, Philippe Druart, Sergio Ochatt, Jean-pierre BaudoinAbstract:The success of interspecific breeding between Phaseolus vulgaris L. (PV) and the two donor species Phaseolus coccineus L. (PC) or Phaseolus polyanthus Greenm. (PP) requires the utilization of the donor species as female parents. Although incompatibility barriers are post-zygotic, success in such F1 crosses is very limited due to early hybrid embryo abortion. Rescue techniques for globular or early heart-shaped embryos have been improved but hybrid plant regeneration remains very difficult. In this study we describe the use of protoplast fusion techniques within the genus Phaseolus, as an alternative to succeed crosses between PP or PC and PV. Large numbers of heterokaryons have been produced using different genotypes and procedures for fusion, based either on electro-fusion (750 or 1500 V.cm-1), or on the use of a chemical micro-method with polyethylene glycol (PEG 6000) as the fusing agent. Both divisions of heterokaryons and the formation of heterokaryon-derived microcalli were observed.
-
Embryogenèse précoce comparative lors des croisements entre Phaseolus coccineus L. et Phaseolus vulgaris L.
Biotechnologie Agronomie Société et Environnement, 2007Co-Authors: Pamphile Nguema Ndoutoumou, André Toussaint, Jean-pierre BaudoinAbstract:Comparative early embryogenesis in crossings between Phaseolus coccineus L. and Phaseolus vulgaris L. Theinterspecifi c hybridization between Phaseolus coccineus L. and Phaseolus vulgaris L. is useful for the genetic improvementof the common bean. The use of the P. vulgaris cytoplasm for such hybridizations leads usually to a rather fast return tothe maternal form in the subsequent generations. When P. vulgaris is the pollinator, crosses result in early embryo abortion(globular or heart-shaped embryos). A competition between the endosperm and the embryo, on the one hand, and between thesuspensor and the embryo, on the other hand, could generate diffi culties of feeding young embryos. Histological sections usingthe 2-Hydroxyethyl Methacrylate (HEMA) resin method on the embryos of P. coccineus (NI16) and P. vulgaris (NI637 andX707) genotypes, as well as on their genotypic combination (NI16 × NI637 and NI16 × X707 and reciprocal crosses) enableus to explain partially these abortion cases. Observations concern embryos from 3 to 6 days after pollination (DAP). Embryodevelopment (suspensor and embryo proper) of the hybrids is slower than that of the parents whatever the crossing. Ingrowthsof suspensor basal cells observed when P. coccineus is the maternal parent are characteristic of the presence of the cytoplasmof these species. Endothelium deterioration (or proliferation) in hybrid embryos would rather be related to the degree reachedby the process of abortion in the embryo concerned.
Alfonso Delgado-salinas - One of the best experts on this subject based on the ideXlab platform.
-
A new species of Phaseolus (Leguminosae, Papilionoideae) sister to Phaseolus vulgaris , the common bean
Phytotaxa, 2017Co-Authors: Martha Rendón-anaya, Alfredo Herrera-estrella, Paul Gepts, Alfonso Delgado-salinasAbstract:Integrating the information provided by different lines of evidence generated in previous studies, Phaseolus debouckii , a new species is described and illustrated. This species occurs only in central-southern Ecuador and north western Peru, mostly in what is known as the Amatope-Huancabamba Depression, and represents a sister species to Phaseolus vulgaris L. A comparison table with quantitative and qualitative morphological characters is given, as well as a distribution map and ecological information.
-
A study of the polen of Phaseolus chiapasanus Piper (Leguminosae, Phaseolinae)
Boletin De La Sociedad Botanica De Mexico, 2017Co-Authors: Alfonso Delgado-salinas, Enrique Martínez-hernández, Pilar Fernández-ortuñoAbstract:In the course of a taxonomical revision of the genus Phaseolus and considering the recently important research realized in the Phaseolus-Vigna complex, where the palynological attributes for the genus were precised, the present paper describes the pollen of Phaseolus chiapasanus Piper. The large-size class pollen grains of Ph. chiapasanus are tricolporate, brevicolpate, pseudo-operculate; semitectate, and widely reticulate. The structure of the infratectum could be considered of the granular-collumelar type. Some of the attributes found in the polen of Ph. Chiapasanus were not known for the genus, though such attributes are commonly found in the subgenus Sigmoidotropis of the genus Vigna. Ph. chiapasanus shows a series of morphological and palynological differences that open the possibility for the creation of a new section within the genus. More detailed studies will definitely by needed to better document relationships to other species of the genus.
-
Karyotypic analysis in six species of Phaseolus L. (FABACEAE)
Caryologia, 2014Co-Authors: Mercado-ruaro Pedro, Alfonso Delgado-salinasAbstract:Abstract The karyotypes of six species of Phaseolus were analyzed. The chromosome complements consist exclusively of metacentric and submetacentrics, their size ranging from 0.70 μm in P. microcarpus to 1.60 μm in P. coccineus. A suggested path of karyologic change is through an increment in the chromatin’s total length. Based upon their karyotypic formula and centromeric index, Phaseolus xanthotrichus is considered the most evolved species, and P. microcarpus the least advanced.
-
New Species of Mexican Phaseolus (Fabaceae)
Systematic Botany, 2000Co-Authors: Alfonso Delgado-salinasAbstract:Abstract Descriptions, keys, distribution, ecology, and notes on taxonomic problems and comparisons with related taxa are provided for seven new species of Phaseolus from Mexico. The new species are classified in informal Phaseolus groups, in accordance with a recent phylogenetic analysis using molecular and non-molecular characters. Phaseolus macvaughii and P. perplexus are members of the P. leptostachyus group and P. pauciflorus group, respectively, Phaseolus juquilensis, P. marechalii, and P. xolocotzii are part of the P. polystachios group, and P. hintonii and P. zimapanensis, form a well-supported clade with P. xanthotrichus, within the P. tuerckheimii group. Communicating Editor: Matt Lavin
-
Phylogenetic analysis of the cultivated and wild species of Phaseolus (Fabaceae)
Systematic Botany, 1999Co-Authors: Alfonso Delgado-salinas, Tom Turley, Adam D. Richman, Matt LavinAbstract:The species of Phaseolus were exhaustively sampled for both ITS/5.8S DNA sequence and non-molecular data. With all related New World genera designated as outgroups, a phylogenetic analysis of combined data reveals a strongly supported monophyletic Phaseolus. Other well supported relationships include nine monophyletic species clades within Phaseolus, designated as the P vulgaris, P filiformis, P lunatus, P polystachios, P leptostachyus, P pauciflorus, P tuerckheimii, and P pedicellatus groups, and P microcarpus. Only the last of these is monotypic and consistently resolved in a sensitivity analysis as the earliest branch in the Phaseolus clade, though with poor bootstrap support. The five most commonly domesticated species in the genus arise from within the P vulgaris and P lunatus groups. The "gene pools" traditionally recognized for the domesticated species P vulgaris and P lunatus are not detected with ITS sequence variation. This is in spite of a very high degree of interand intra-specific ITS sequence divergence in Phaseolus. Though the genus Phaseolus has a complex taxonomic and nomenclatural history, recent circumscriptions of the genus are narrow (e.g., Verdcourt 1970) and most of the species with uncertain affinities have been relegated to Vigna. Vigna rather than Phaseolus has become the large "catch-all" pantropcial genus (Marechal 1982; Delgado-Salinas 1985; McVaugh 1987). In this modern circumscription, Phaseolus is strictly New World, concentrated in tropical and warm temperate North America, and diagnosed by foliage bearing hooked hairs, keel petals that are laterally and tightly coiled, and inflorescence nodes that lack extrafloral nectaries (Baudet 1977; Lackey 1978, 1981, 1983). Still retained in the genus are five economically important species: P acutifolius (tepary bean), P coccineus (scarlet runner bean), P lunatus (lima bean), P polyanthus (year bean), and P vulgaris (common bean). Little has been done to test the hypothesis of monophyly for the modern circumscription of Phaseolus. The traits supposedly diagnostic of Phaseolus occur in species of other genera (e.g., Vigna adenantha has laterally coiled keel petals, and species of Dolichos and Dipogon have inflorescence nodes lacking nectaries). A phylogenetic analysis of chloroplast DNA restriction site data suggests that Phaseolus is monophyletic (Delgado-Salinas et al. 1993). Taxon sampling, however, was not addressed, and this is a concern given that only 10 of approximately 50 species in the genus were sampled. Phaseolus is suggested to be part of a weakly supported monophyletic group of about 10 Old and New World genera that have poorly resolved relationships (Doyle and Doyle 1993; Bruneau et al. 1995). However, other studies suggest that the sister group relationships of Phaseolus include only New World genera. Analysis of chloroplast DNA restriction sites (Delgado-Salinas et al. 1993) resolved a New World clade where the genera Macroptilium and Strophostyles were sister to Phaseolus. A close relationship of Phaseolus to other New World genera is also suggested by an additional analysis of chloroplast DNA restriction sites (Vaillancourt et al. 1993-a study that focused on Vigna), a phenetic analysis of morphological data (Marechal et al. 1978, 1981), and a traditional taxonomic classification (Lackey 1981, 1983). A comprehensive phylogeny including both the wild and cultivated species of Phaseolus has been neglected, possibly because of an emphasis on the study of the domesticated species. What is known comes from phenetic and cladistic analysis of isozyme banding profiles (Jaaska 1996), phenetic analysis of morphological characters (Marechal et al. 1978), and cladistic analysis of chloroplast DNA restriction site data (Delgado-Salinas et al. 1993; Llaca et al. 1994). Yet character or taxon sampling has been insufficient to make any definitive conclusions about the infra-generic phylogenetic relationships
Ana Christina Brasileiro-vidal - One of the best experts on this subject based on the ideXlab platform.
-
Breaks of macrosynteny and collinearity among moth bean (Vigna aconitifolia), cowpea (V. unguiculata), and common bean (Phaseolus vulgaris)
Chromosome Research, 2020Co-Authors: Ana Rafaela Da S. Oliveira, Andrea Pedrosa-harand, Lívia Do Vale Martins, Fernanda De O. Bustamante, María Muñoz-amatriaín, Timothy Close, Antônio F. Costa, Ana Maria Benko-iseppon, Ana Christina Brasileiro-vidalAbstract:Comparative cytogenetic mapping is a powerful approach to gain insights into genome organization of orphan crops, lacking a whole sequenced genome. To investigate the cytogenomic evolution of important Vigna and Phaseolus beans, we built a BAC-FISH (fluorescent in situ hybridization of bacterial artificial chromosome) map of Vigna aconitifolia ( Vac , subgenus Ceratotropis ), species with no sequenced genome, and compared with V. unguiculata ( Vu , subgenus Vigna ) and Phaseolus vulgaris ( Pv ) maps. Seventeen Pv BACs, eight Vu BACs, and 5S and 35S rDNA probes were hybridized in situ on the 11 Vac chromosome pairs. Five Vac chromosomes ( Vac 6, Vac 7, Vac 9, Vac 10, and Vac 11) showed conserved macrosynteny and collinearity between V. unguiculata and P. vulgaris . On the other hand, we observed collinearity breaks, identified by pericentric inversions involving Vac 2 ( Vu 2), Vac 4 ( Vu 4), and Vac 3 ( Pv 3). We also detected macrosynteny breaks of translocation type involving chromosomes 1 and 8 of V. aconitifolia and P. vulgaris ; 2 and 3 of V. aconitifolia and P. vulgaris ; and 1 and 5 of V. aconitifolia and V. unguiculata . Considering our data and previous BAC-FISH studies, six chromosomes (1, 2, 3, 4, 5, and 8) are involved in major karyotype divergences between genera and five (1, 2, 3, 4, and 5) between Vigna subgenera, including mechanisms such as duplications, inversions, and translocations. Macrosynteny breaks between Vigna and Phaseolus suggest that the major chromosomal rearrangements have occurred within the Vigna clade. Our cytogenomic comparisons bring new light on the degree of shared macrosynteny and mechanisms of karyotype diversification during Vigna and Phaseolus evolution.
