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Chao-chi Huang - One of the best experts on this subject based on the ideXlab platform.
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:(Received 10 April 19% - Revised 22 July 19% - Accepted 25 July 19%) Studies in autoimmune-prone NZBlW F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZBm F1 mice were fed on diets containing 200 g dietary fat& and 50 g dietary fatkg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fatkg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18%-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 34 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases. Systemic lupus erythematosus: Autoimmunity: Cardiolipin
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:Studies in autoimmune-prone NZB/W F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZB/W F1 mice were fed on diets containing 200 g dietary fat/kg and 50 g dietary fat/kg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fat/kg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18:2n-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 3-4 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases.
Bor-luen Chiang - One of the best experts on this subject based on the ideXlab platform.
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increased transforming growth factor beta tgf β secreting t cells and iga anti cardiolipin antibody levels during acute stage of childhood henoch schonlein purpura
Clinical and Experimental Immunology, 2000Co-Authors: Yaohsu Yang, Miaotzu Huang, Shurung Lin, Yutsan Lin, M J Tsai, Bor-luen ChiangAbstract:Henoch–Schonlein purpura (HSP) is a small vessel vasculitis characterized by increased serum IgA and IgA-dominant immune complex deposition in lesions. The involvement of IgA implies a probable role for TGF-β, a major factor in IgA production, in the pathogenesis of HSP. Among IgA Antibodies, serum IgA Anti-Cardiolipin Antibodies (aCL) have been found in many diseases, including vasculitis. In addition to the clinical presentations and laboratory parameters, we further investigated the roles of IgA aCL and TGF-β in childhood HSP. Twenty-six Chinese children with the diagnosis of HSP were enrolled. Blood samples from these patients were collected at both acute and convalescent stages. Intracellular staining of lymphocytes was performed to enumerate type 1 (interferon-gamma-secreting), type 2 (IL-4-secreting), and type 3 (TGF-β-secreting) helper T cells. Serum levels of TGF-β were detected by ELISA. Serum IgA aCL of 21 of 26 patients at the acute stage, 11 of them at the convalescent stage, were measured by ELISA. The data showed that IgA aCL serum levels were significantly elevated in patients compared with healthy controls (P < 0·001), and those patients at the convalescent stage (P < 0·001). In addition, TGF-β-secreting T cells were significantly elevated during the acute stage, and decreased at the convalescent stage. Although more studies are needed, the high prevalence of IgA aCL and increased TGF-β-secreting T cells in children with acute HSP revealed some points which should permit a better understanding of the pathogenesis of HSP.
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:(Received 10 April 19% - Revised 22 July 19% - Accepted 25 July 19%) Studies in autoimmune-prone NZBlW F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZBm F1 mice were fed on diets containing 200 g dietary fat& and 50 g dietary fatkg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fatkg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18%-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 34 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases. Systemic lupus erythematosus: Autoimmunity: Cardiolipin
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:Studies in autoimmune-prone NZB/W F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZB/W F1 mice were fed on diets containing 200 g dietary fat/kg and 50 g dietary fat/kg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fat/kg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18:2n-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 3-4 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases.
Bi-fong Lin - One of the best experts on this subject based on the ideXlab platform.
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:(Received 10 April 19% - Revised 22 July 19% - Accepted 25 July 19%) Studies in autoimmune-prone NZBlW F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZBm F1 mice were fed on diets containing 200 g dietary fat& and 50 g dietary fatkg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fatkg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18%-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 34 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases. Systemic lupus erythematosus: Autoimmunity: Cardiolipin
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:Studies in autoimmune-prone NZB/W F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZB/W F1 mice were fed on diets containing 200 g dietary fat/kg and 50 g dietary fat/kg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fat/kg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18:2n-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 3-4 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases.
Eli S Gray - One of the best experts on this subject based on the ideXlab platform.
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antibody specificities associated with neutralization breadth in plasma from human immunodeficiency virus type 1 subtype c infected blood donors
Journal of Virology, 2009Co-Authors: Eli S Gray, Natasha Taylo, Diane Wycuff, Penny L Moore, Georgia D Tomaras, Constantinos Ku Wibme, Adria Pure, Alla C Decamp, Pete Gilbe, Lake WoodAbstract:Defining the specificities of the anti-human immunodeficiency virus type 1 (HIV-1) envelope Antibodies able to mediate broad heterologous neutralization will assist in identifying targets for an HIV-1 vaccine. We screened 70 plasmas from chronically HIV-1-infected individuals for neutralization breadth. Of these, 16 (23%) were found to neutralize 80% or more of the viruses tested. Anti-CD4 binding site (CD4bs) Antibodies were found in almost all plasmas independent of their neutralization breadth, but they mainly mediated neutralization of the laboratory strain HxB2 with little effect on the primary virus, Du151. Adsorption with Du151 monomeric gp120 reduced neutralizing activity to some extent in most plasma samples when tested against the matched virus, although these Antibodies did not always confer cross-neutralization. For one plasma, this activity was mapped to a site overlapping the CD4-induced (CD4i) epitope and CD4bs. Anti-membrane-proximal external region (MPER) (r = 0.69; P < 0.001) and anti-CD4i (r = 0.49; P < 0.001) antibody titers were found to be correlated with the neutralization breadth. These anti-MPER Antibodies were not 4E10- or 2F5-like but spanned the 4E10 epitope. Furthermore, we found that Anti-Cardiolipin Antibodies were correlated with the neutralization breadth (r = 0.67; P < 0.001) and anti-MPER Antibodies (r = 0.6; P < 0.001). Our study suggests that more than one epitope on the envelope glycoprotein is involved in the cross-reactive neutralization elicited during natural HIV-1 infection, many of which are yet to be determined, and that polyreactive Antibodies are possibly involved in this phenomenon.
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antibody specificities associated with neutralization breadth in plasma from human immunodeficiency virus type 1 subtype c infected blood donors
Journal of Virology, 2009Co-Authors: Eli S Gray, Diane Wycuff, Penny L Moore, Georgia D Tomaras, Alla C Decamp, Natasha Taylor, Constantinos Kurt Wibmer, Adrian Puren, Peter B Gilbert, Blake WoodAbstract:Defining the specificities of the anti-human immunodeficiency virus type 1 (HIV-1) envelope Antibodies able to mediate broad heterologous neutralization will assist in identifying targets for an HIV-1 vaccine. We screened 70 plasmas from chronically HIV-1-infected individuals for neutralization breadth. Of these, 16 (23%) were found to neutralize 80% or more of the viruses tested. Anti-CD4 binding site (CD4bs) Antibodies were found in almost all plasmas independent of their neutralization breadth, but they mainly mediated neutralization of the laboratory strain HxB2 with little effect on the primary virus, Du151. Adsorption with Du151 monomeric gp120 reduced neutralizing activity to some extent in most plasma samples when tested against the matched virus, although these Antibodies did not always confer cross-neutralization. For one plasma, this activity was mapped to a site overlapping the CD4-induced (CD4i) epitope and CD4bs. Anti-membrane-proximal external region (MPER) (r = 0.69; P < 0.001) and anti-CD4i (r = 0.49; P < 0.001) antibody titers were found to be correlated with the neutralization breadth. These anti-MPER Antibodies were not 4E10- or 2F5-like but spanned the 4E10 epitope. Furthermore, we found that Anti-Cardiolipin Antibodies were correlated with the neutralization breadth (r = 0.67; P < 0.001) and anti-MPER Antibodies (r = 0.6; P < 0.001). Our study suggests that more than one epitope on the envelope glycoprotein is involved in the cross-reactive neutralization elicited during natural HIV-1 infection, many of which are yet to be determined, and that polyreactive Antibodies are possibly involved in this phenomenon.
Su-jen Jeng - One of the best experts on this subject based on the ideXlab platform.
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:(Received 10 April 19% - Revised 22 July 19% - Accepted 25 July 19%) Studies in autoimmune-prone NZBlW F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZBm F1 mice were fed on diets containing 200 g dietary fat& and 50 g dietary fatkg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fatkg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18%-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 34 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases. Systemic lupus erythematosus: Autoimmunity: Cardiolipin
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dietary fat affects lipids and anti cardiolipin antibody levels in autoimmune prone nzb w f1 mice
British Journal of Nutrition, 1997Co-Authors: Bi-fong Lin, Bor-luen Chiang, Su-jen Jeng, Chao-chi HuangAbstract:Studies in autoimmune-prone NZB/W F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-Cardiolipin Antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and Anti-Cardiolipin antibody production in autoimmune-prone mice. Two groups of NZB/W F1 mice were fed on diets containing 200 g dietary fat/kg and 50 g dietary fat/kg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and Anti-Cardiolipin Antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fat/kg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18:2n-6) and arachidonic acid (20:4n-6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM Anti-Cardiolipin antibody levels between these two groups. However, IgG Anti-Cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 3-4 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases.