The Experts below are selected from a list of 346677 Experts worldwide ranked by ideXlab platform
Francisco J. Arregui - One of the best experts on this subject based on the ideXlab platform.
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Celiac disease biodetection using lossy-mode resonances generated in tapered single-mode optical fibers
23rd International Conference on Optical Fibre Sensors, 2014Co-Authors: Abian B. Socorro, Jesus M. Corres, Ignacio R. Matias, I. Del Villar, Francisco J. ArreguiAbstract:This work presents the development and test of an Anti-Gliadin Antibodies biosensor based on lossy mode resonances (LMRs) to detect celiac disease. Several polyelectrolites were used to perform layer-by-layer assembly processes in order to generate the LMR and to fabricate a gliadin-embedded thin-film. The LMR shifted 20 nm when immersed in a 5 ppm Anti-Gliadin Antibodies-PBS solution, what makes this bioprobe suitable for detecting celiac disease. This is the first time, to our knowledge, that LMRs are used to detect celiac disease and these results suppose promising prospects on the use of such phenomena as biological detectors.
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Spectral width reduction in lossy mode resonance-based sensors by means of tapered optical fibre structures
Sensors and Actuators B: Chemical, 2014Co-Authors: Abian B. Socorro, Jesus M. Corres, Francisco J. Arregui, I. Del Villar, Ignacio R. MatiasAbstract:Abstract This contribution pursues the goal of achieving an optical fibre-based platform to detect Anti-Gliadin Antibodies (AGAs), in order to early diagnose celiac disease. To this purpose, the generation of lossy mode resonances (LMRs) in several evanescent field optical structures was studied both theoretically and experimentally. LMRs were obtained by adsorbing a polymeric thin-film onto the optical structures using the layer-by-layer assembly technique. The LMR shape depends on the geometry of the optical structure and its attenuation was controlled just by tuning the length of the device. The best performance was obtained with tapered single-mode optical fibres, which provided more than a 50% reduction in the spectral width of the LMR by using a shorter device. This improvement was successfully applied to detect Anti-Gliadin Antibodies (AGAs) in 5 ppm concentration, what can be used to diagnose celiac disease, and shows the potential of this technology to address biosensing applications.
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Tapered optical fiber biosensor for the detection of Anti-Gliadin Antibodies
Sensors and Actuators B: Chemical, 2008Co-Authors: Jesus M. Corres, Ignacio R. Matias, J. Bravo, Francisco J. ArreguiAbstract:Abstract A new biosensor has been developed by coating a tapered optical fiber with an Anti-Gliadin Antibodies (AGAs) sensitive nanofilm using the electrostatic self-assembled (ESA) monolayer technique in order to aid the diagnosis of celiac disease. Optical fibers were tapered and then the specific antigen was deposited using the ESA method which allows the construction of nanometric scale recognition surfaces on the fiber optic and helps to create fast response sensors for real time observation of the binding process. Power changes up to 6 dB have been recorded during the Antibodies binding process with concentrations in the range 1–15 ppm. Optimal deposition parameters have been selected using an in situ interferometric characterization technique which allows to state the working point of the sensor accurately. The high sensitivity and continuous monitoring of the proposed scheme can reduce importantly the time and serum volume required for celiac disease tests.
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Tapered Optical Fiber Biosensor for the Detection of Anti-Gliadin Antibodies
2007 IEEE Sensors, 2007Co-Authors: Jesus M. Corres, Ignacio R. Matias, J. Bravo, Francisco J. ArreguiAbstract:In this paper a new fiber optic sensor for the detection of Antibodies to gliadin, in order to aid the diagnosis of celiac disease, is presented. Optical fibers of 200/230 mum (core and cladding diameters respectively) were tapered to a waist diameter in the range of 15-20 mum, and then the specific antigen was deposited using the Electrostatic Self-Assembly (ESA) method. Optimal deposition parameters have been selected using an in-situ interferometric characterization technique. The high sensitivity and continuous monitoring of the proposed scheme can reduce importantly the time and serum volume required for celiac disease tests. Performance time is shorter due to the absence of many washing and blocking steps as needed in conventional methods. Additionally, the ESA method allows the construction of nanometric scale recognition surfaces on the fiber optic, which helps to create fast response sensors for real time observation of the binding process.
Torsten Matthias - One of the best experts on this subject based on the ideXlab platform.
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Diagnostic challenges in celiac disease and the role of the tissue transglutaminase-neo-epitope.
Clinical reviews in allergy & immunology, 2009Co-Authors: Torsten Matthias, Sascha Pfeiffer, Carlo Selmi, M Eric GershwinAbstract:The diagnosis of celiac disease (CD) remains a clinical challenge based on the incomplete specificity and sensitivity rates of current non-invasive tests. Furthermore, histological assessments fail to identify all overt cases and, in particular, do not manifest pathognomonic alterations in silent cases. Accordingly, the majority of CD cases are diagnosed with great delay. Recent research into the pathogenesis of CD, allowed us to identify a neo-antigen that appears to be the most promising serological tool for the detection of anti-tissue transglutaminase as well as Anti-Gliadin Antibodies.
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Diagnostic Challenges in Celiac Disease and the Role of the Tissue Transglutaminase–Neo-Epitope
Clinical Reviews in Allergy & Immunology, 2009Co-Authors: Torsten Matthias, Sascha Pfeiffer, Carlo Selmi, M. Eric GershwinAbstract:The diagnosis of celiac disease (CD) remains a clinical challenge based on the incomplete specificity and sensitivity rates of current non-invasive tests. Furthermore, histological assessments fail to identify all overt cases and, in particular, do not manifest pathognomonic alterations in silent cases. Accordingly, the majority of CD cases are diagnosed with great delay. Recent research into the pathogenesis of CD, allowed us to identify a neo-antigen that appears to be the most promising serological tool for the detection of anti-tissue transglutaminase as well as Anti-Gliadin Antibodies.
Jesus M. Corres - One of the best experts on this subject based on the ideXlab platform.
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Celiac disease biodetection using lossy-mode resonances generated in tapered single-mode optical fibers
23rd International Conference on Optical Fibre Sensors, 2014Co-Authors: Abian B. Socorro, Jesus M. Corres, Ignacio R. Matias, I. Del Villar, Francisco J. ArreguiAbstract:This work presents the development and test of an Anti-Gliadin Antibodies biosensor based on lossy mode resonances (LMRs) to detect celiac disease. Several polyelectrolites were used to perform layer-by-layer assembly processes in order to generate the LMR and to fabricate a gliadin-embedded thin-film. The LMR shifted 20 nm when immersed in a 5 ppm Anti-Gliadin Antibodies-PBS solution, what makes this bioprobe suitable for detecting celiac disease. This is the first time, to our knowledge, that LMRs are used to detect celiac disease and these results suppose promising prospects on the use of such phenomena as biological detectors.
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Spectral width reduction in lossy mode resonance-based sensors by means of tapered optical fibre structures
Sensors and Actuators B: Chemical, 2014Co-Authors: Abian B. Socorro, Jesus M. Corres, Francisco J. Arregui, I. Del Villar, Ignacio R. MatiasAbstract:Abstract This contribution pursues the goal of achieving an optical fibre-based platform to detect Anti-Gliadin Antibodies (AGAs), in order to early diagnose celiac disease. To this purpose, the generation of lossy mode resonances (LMRs) in several evanescent field optical structures was studied both theoretically and experimentally. LMRs were obtained by adsorbing a polymeric thin-film onto the optical structures using the layer-by-layer assembly technique. The LMR shape depends on the geometry of the optical structure and its attenuation was controlled just by tuning the length of the device. The best performance was obtained with tapered single-mode optical fibres, which provided more than a 50% reduction in the spectral width of the LMR by using a shorter device. This improvement was successfully applied to detect Anti-Gliadin Antibodies (AGAs) in 5 ppm concentration, what can be used to diagnose celiac disease, and shows the potential of this technology to address biosensing applications.
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Tapered optical fiber biosensor for the detection of Anti-Gliadin Antibodies
Sensors and Actuators B: Chemical, 2008Co-Authors: Jesus M. Corres, Ignacio R. Matias, J. Bravo, Francisco J. ArreguiAbstract:Abstract A new biosensor has been developed by coating a tapered optical fiber with an Anti-Gliadin Antibodies (AGAs) sensitive nanofilm using the electrostatic self-assembled (ESA) monolayer technique in order to aid the diagnosis of celiac disease. Optical fibers were tapered and then the specific antigen was deposited using the ESA method which allows the construction of nanometric scale recognition surfaces on the fiber optic and helps to create fast response sensors for real time observation of the binding process. Power changes up to 6 dB have been recorded during the Antibodies binding process with concentrations in the range 1–15 ppm. Optimal deposition parameters have been selected using an in situ interferometric characterization technique which allows to state the working point of the sensor accurately. The high sensitivity and continuous monitoring of the proposed scheme can reduce importantly the time and serum volume required for celiac disease tests.
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Tapered Optical Fiber Biosensor for the Detection of Anti-Gliadin Antibodies
2007 IEEE Sensors, 2007Co-Authors: Jesus M. Corres, Ignacio R. Matias, J. Bravo, Francisco J. ArreguiAbstract:In this paper a new fiber optic sensor for the detection of Antibodies to gliadin, in order to aid the diagnosis of celiac disease, is presented. Optical fibers of 200/230 mum (core and cladding diameters respectively) were tapered to a waist diameter in the range of 15-20 mum, and then the specific antigen was deposited using the Electrostatic Self-Assembly (ESA) method. Optimal deposition parameters have been selected using an in-situ interferometric characterization technique. The high sensitivity and continuous monitoring of the proposed scheme can reduce importantly the time and serum volume required for celiac disease tests. Performance time is shorter due to the absence of many washing and blocking steps as needed in conventional methods. Additionally, the ESA method allows the construction of nanometric scale recognition surfaces on the fiber optic, which helps to create fast response sensors for real time observation of the binding process.
Ignacio R. Matias - One of the best experts on this subject based on the ideXlab platform.
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Celiac disease biodetection using lossy-mode resonances generated in tapered single-mode optical fibers
23rd International Conference on Optical Fibre Sensors, 2014Co-Authors: Abian B. Socorro, Jesus M. Corres, Ignacio R. Matias, I. Del Villar, Francisco J. ArreguiAbstract:This work presents the development and test of an Anti-Gliadin Antibodies biosensor based on lossy mode resonances (LMRs) to detect celiac disease. Several polyelectrolites were used to perform layer-by-layer assembly processes in order to generate the LMR and to fabricate a gliadin-embedded thin-film. The LMR shifted 20 nm when immersed in a 5 ppm Anti-Gliadin Antibodies-PBS solution, what makes this bioprobe suitable for detecting celiac disease. This is the first time, to our knowledge, that LMRs are used to detect celiac disease and these results suppose promising prospects on the use of such phenomena as biological detectors.
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Spectral width reduction in lossy mode resonance-based sensors by means of tapered optical fibre structures
Sensors and Actuators B: Chemical, 2014Co-Authors: Abian B. Socorro, Jesus M. Corres, Francisco J. Arregui, I. Del Villar, Ignacio R. MatiasAbstract:Abstract This contribution pursues the goal of achieving an optical fibre-based platform to detect Anti-Gliadin Antibodies (AGAs), in order to early diagnose celiac disease. To this purpose, the generation of lossy mode resonances (LMRs) in several evanescent field optical structures was studied both theoretically and experimentally. LMRs were obtained by adsorbing a polymeric thin-film onto the optical structures using the layer-by-layer assembly technique. The LMR shape depends on the geometry of the optical structure and its attenuation was controlled just by tuning the length of the device. The best performance was obtained with tapered single-mode optical fibres, which provided more than a 50% reduction in the spectral width of the LMR by using a shorter device. This improvement was successfully applied to detect Anti-Gliadin Antibodies (AGAs) in 5 ppm concentration, what can be used to diagnose celiac disease, and shows the potential of this technology to address biosensing applications.
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Tapered optical fiber biosensor for the detection of Anti-Gliadin Antibodies
Sensors and Actuators B: Chemical, 2008Co-Authors: Jesus M. Corres, Ignacio R. Matias, J. Bravo, Francisco J. ArreguiAbstract:Abstract A new biosensor has been developed by coating a tapered optical fiber with an Anti-Gliadin Antibodies (AGAs) sensitive nanofilm using the electrostatic self-assembled (ESA) monolayer technique in order to aid the diagnosis of celiac disease. Optical fibers were tapered and then the specific antigen was deposited using the ESA method which allows the construction of nanometric scale recognition surfaces on the fiber optic and helps to create fast response sensors for real time observation of the binding process. Power changes up to 6 dB have been recorded during the Antibodies binding process with concentrations in the range 1–15 ppm. Optimal deposition parameters have been selected using an in situ interferometric characterization technique which allows to state the working point of the sensor accurately. The high sensitivity and continuous monitoring of the proposed scheme can reduce importantly the time and serum volume required for celiac disease tests.
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Tapered Optical Fiber Biosensor for the Detection of Anti-Gliadin Antibodies
2007 IEEE Sensors, 2007Co-Authors: Jesus M. Corres, Ignacio R. Matias, J. Bravo, Francisco J. ArreguiAbstract:In this paper a new fiber optic sensor for the detection of Antibodies to gliadin, in order to aid the diagnosis of celiac disease, is presented. Optical fibers of 200/230 mum (core and cladding diameters respectively) were tapered to a waist diameter in the range of 15-20 mum, and then the specific antigen was deposited using the Electrostatic Self-Assembly (ESA) method. Optimal deposition parameters have been selected using an in-situ interferometric characterization technique. The high sensitivity and continuous monitoring of the proposed scheme can reduce importantly the time and serum volume required for celiac disease tests. Performance time is shorter due to the absence of many washing and blocking steps as needed in conventional methods. Additionally, the ESA method allows the construction of nanometric scale recognition surfaces on the fiber optic, which helps to create fast response sensors for real time observation of the binding process.
Patrizia Restani - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and Immunochemical Evidences Supporting the Inclusion of Quinoa (Chenopodium quinoa Willd.) as a Gluten-free Ingredient
Plant Foods for Human Nutrition, 2014Co-Authors: Elena Peñas, Francesca Uberti, Chiara Lorenzo, Cinzia Ballabio, Andrea Brandolini, Patrizia RestaniAbstract:To date, the only acceptable therapeutic approach for celiac disease (CD) is a strict elimination from the diet of gluten-containing foods, but this diet does not always guarantee an adequate nutritional intake. Pseudocereals are receiving considerable attention as interesting alternatives for the formulation of gluten-free products, and quinoa grains arise as nutritive substitutes of conventional cereals. The aim of this study was the characterization of different quinoa samples corresponding to 11 quinoa varieties, using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) and immunoblotting techniques to assess their suitability for celiac subjects. Some of these varieties were grown in Italy to assess if the reproduction in a new habitat can guarantee the retention of the “safe” protein pattern. None of the quinoa varieties studied presented protein bands with electrophoretic mobility comparable with those of wheat gliadins, the toxic protein for celiac subjects. All the quinoa samples showed a low binding affinity for both specific Anti-Gliadin Antibodies and IgAs from celiac subjects, confirming that quinoa can be considered as a safe ingredient for celiac patients. However, reliable varieties should be previously selected since the immuno cross-reactivity with Anti-Gliadin Antibodies can vary significantly.
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Diversity of oat varieties in eliciting the early inflammatory events in celiac disease
European Journal of Nutrition, 2014Co-Authors: Marco Silano, Francesca Uberti, Elena Penas Pozo, Sara Manferdelli, Tamara Del Pinto, Cristina Felli, Andrea Budelli, Olimpia Vincentini, Patrizia RestaniAbstract:Purpose Celiac disease (CD) is an autoimmune enteropathy, triggered by dietary gluten. The only treatment is a strict gluten-free diet. Oats are included in the list of gluten-free ingredients by European Regulation, but the safety of oats in CD is still a matter of debate. The present study examined the capability of different oat cultivars of activating the gliadin-induced transglutaminase-2 (TG2)-dependent events in some in vitro models of CD. In addition, we compared this capability with the electrophoresis pattern of peptic–tryptic digests of the proteins of the oat cultivars. Methods K562(S) cells agglutination, transepithelial electrical resistance of T84-cell monolayers, intracellular levels of TG2 and phosphorylated form of protein 42–44 in T84 cells were the early gliadin-dependent events studied. Results The results showed that the Nave oat cultivar elicited these events, whereas Irina and Potenza varieties did not. The ability of a cultivar to activate the above-described events was associated with the electrophoretic pattern of oat proteins and their reactivity to Anti-Gliadin Antibodies. Conclusion We found significant differences among oat cultivars in eliciting the TG2-mediated events of CD inflammation. Therefore, the safety of an oat cultivar in CD might be screened in vitro by means of biochemical and biological assays, before starting a clinical trial to definitely assess its safety.
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Biochemical and immunochemical characterization of different varieties of amaranth (Amaranthus L. ssp.) as a safe ingredient for gluten-free products.
Journal of agricultural and food chemistry, 2011Co-Authors: Cinzia Ballabio, Elena Peñas, Francesca Uberti, Chiara Lorenzo, Andrea Brandolini, Patrizia RestaniAbstract:Celiac disease is a food intolerance triggered by the ingestion of gluten-containing cereals; the only therapy is a strict gluten-free diet for life. In recent years, amaranth flour has received considerable attention as an interesting source for the formulation of gluten-free products due to its high nutritional value and low content of prolamins, the toxic proteins for celiacs. The aim of this study was to characterize 40 amaranth varieties using both SDS-PAGE/immunoblotting and ELISA to assess their possible tolerance by celiac subjects. All of the amaranth samples studied showed similar binding affinities for both specific Anti-Gliadin Antibodies and human IgAs. In most amaranth grains, the content of gluten-like proteins measured by ELISA was
