The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform
Fengquan Liu - One of the best experts on this subject based on the ideXlab platform.
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Hfq regulates antibacterial Antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11
Microbial biotechnology, 2015Co-Authors: Yuxin Zhao, Guoliang Qian, Fengquan LiuAbstract:Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel Antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial Antibiotic biosynthesis and extracellular lytic enzyme activity in L. enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial Antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L. enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in Antibiotic Metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.
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transcriptomic analysis reveals new regulatory roles of clp signaling in secondary Metabolite biosynthesis and surface motility in lysobacter enzymogenes oh11
Applied Microbiology and Biotechnology, 2014Co-Authors: Yansheng Wang, Guoliang Qian, Yuxin Zhao, Juan Zhang, Yangyang Zhao, Yan Shen, Justin M Huffman, Vittorio Venturi, Fengquan LiuAbstract:Lysobacter enzymogenes is a bacterial biological control agent emerging as a new source of Antibiotic Metabolites, such as heat-stable antifungal factor (HSAF) and the antibacterial factor WAP-8294A2. The regulatory mechanism(s) for Antibiotic Metabolite biosynthesis remains largely unknown in L. enzymogenes. Clp, a cyclic adenosine monophosphate (cAMP)-receptor-like protein, is shown to function as a global regulator in modulating biocontrol-associated traits in L. enzymogenes. However, the genetic basis of Clp signaling remains unclear. Here, we utilized transcriptome/microarray analysis to determine the Clp regulon in L. enzymogenes. We showed that Clp is a global regulator in gene expression, as the transcription of 775 genes belonging to 19 functional groups was differentially controlled by Clp signaling. Analysis of the Clp regulon detected previously characterized Clp-modulated functions as well as novel loci. These include novel loci involved in Antibiotic Metabolite biosynthesis and surface motility in L. enzymogenes. We further showed experimentally that Clp signaling played a positive role in regulating the biosynthesis of HSAF and WAP-8294A2, as well as surface motility which is a type-IV-pilus-dependent trait. The regulation by Clp signaling of Antibiotic (HSAF and WAP-8294A2) biosynthesis and surface motility was found to be independent. Importantly, we identified a factor Lysobacter acetyltransferase (Lat), a homologue of histone acetyltransferase Hpa2, which was regulated by Clp and involved in HSAF biosynthesis, but not associated with WAP-8294A2 production and surface motility. Overall, our study provided new insights into the regulatory role and molecular mechanism of Clp signaling in L. enzymogenes.
Yuxin Zhao - One of the best experts on this subject based on the ideXlab platform.
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Hfq regulates antibacterial Antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11
Microbial biotechnology, 2015Co-Authors: Yuxin Zhao, Guoliang Qian, Fengquan LiuAbstract:Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel Antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial Antibiotic biosynthesis and extracellular lytic enzyme activity in L. enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial Antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L. enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in Antibiotic Metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.
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transcriptomic analysis reveals new regulatory roles of clp signaling in secondary Metabolite biosynthesis and surface motility in lysobacter enzymogenes oh11
Applied Microbiology and Biotechnology, 2014Co-Authors: Yansheng Wang, Guoliang Qian, Yuxin Zhao, Juan Zhang, Yangyang Zhao, Yan Shen, Justin M Huffman, Vittorio Venturi, Fengquan LiuAbstract:Lysobacter enzymogenes is a bacterial biological control agent emerging as a new source of Antibiotic Metabolites, such as heat-stable antifungal factor (HSAF) and the antibacterial factor WAP-8294A2. The regulatory mechanism(s) for Antibiotic Metabolite biosynthesis remains largely unknown in L. enzymogenes. Clp, a cyclic adenosine monophosphate (cAMP)-receptor-like protein, is shown to function as a global regulator in modulating biocontrol-associated traits in L. enzymogenes. However, the genetic basis of Clp signaling remains unclear. Here, we utilized transcriptome/microarray analysis to determine the Clp regulon in L. enzymogenes. We showed that Clp is a global regulator in gene expression, as the transcription of 775 genes belonging to 19 functional groups was differentially controlled by Clp signaling. Analysis of the Clp regulon detected previously characterized Clp-modulated functions as well as novel loci. These include novel loci involved in Antibiotic Metabolite biosynthesis and surface motility in L. enzymogenes. We further showed experimentally that Clp signaling played a positive role in regulating the biosynthesis of HSAF and WAP-8294A2, as well as surface motility which is a type-IV-pilus-dependent trait. The regulation by Clp signaling of Antibiotic (HSAF and WAP-8294A2) biosynthesis and surface motility was found to be independent. Importantly, we identified a factor Lysobacter acetyltransferase (Lat), a homologue of histone acetyltransferase Hpa2, which was regulated by Clp and involved in HSAF biosynthesis, but not associated with WAP-8294A2 production and surface motility. Overall, our study provided new insights into the regulatory role and molecular mechanism of Clp signaling in L. enzymogenes.
Guoliang Qian - One of the best experts on this subject based on the ideXlab platform.
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Hfq regulates antibacterial Antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11
Microbial biotechnology, 2015Co-Authors: Yuxin Zhao, Guoliang Qian, Fengquan LiuAbstract:Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel Antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial Antibiotic biosynthesis and extracellular lytic enzyme activity in L. enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial Antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L. enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in Antibiotic Metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.
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transcriptomic analysis reveals new regulatory roles of clp signaling in secondary Metabolite biosynthesis and surface motility in lysobacter enzymogenes oh11
Applied Microbiology and Biotechnology, 2014Co-Authors: Yansheng Wang, Guoliang Qian, Yuxin Zhao, Juan Zhang, Yangyang Zhao, Yan Shen, Justin M Huffman, Vittorio Venturi, Fengquan LiuAbstract:Lysobacter enzymogenes is a bacterial biological control agent emerging as a new source of Antibiotic Metabolites, such as heat-stable antifungal factor (HSAF) and the antibacterial factor WAP-8294A2. The regulatory mechanism(s) for Antibiotic Metabolite biosynthesis remains largely unknown in L. enzymogenes. Clp, a cyclic adenosine monophosphate (cAMP)-receptor-like protein, is shown to function as a global regulator in modulating biocontrol-associated traits in L. enzymogenes. However, the genetic basis of Clp signaling remains unclear. Here, we utilized transcriptome/microarray analysis to determine the Clp regulon in L. enzymogenes. We showed that Clp is a global regulator in gene expression, as the transcription of 775 genes belonging to 19 functional groups was differentially controlled by Clp signaling. Analysis of the Clp regulon detected previously characterized Clp-modulated functions as well as novel loci. These include novel loci involved in Antibiotic Metabolite biosynthesis and surface motility in L. enzymogenes. We further showed experimentally that Clp signaling played a positive role in regulating the biosynthesis of HSAF and WAP-8294A2, as well as surface motility which is a type-IV-pilus-dependent trait. The regulation by Clp signaling of Antibiotic (HSAF and WAP-8294A2) biosynthesis and surface motility was found to be independent. Importantly, we identified a factor Lysobacter acetyltransferase (Lat), a homologue of histone acetyltransferase Hpa2, which was regulated by Clp and involved in HSAF biosynthesis, but not associated with WAP-8294A2 production and surface motility. Overall, our study provided new insights into the regulatory role and molecular mechanism of Clp signaling in L. enzymogenes.
Hailong Xiao - One of the best experts on this subject based on the ideXlab platform.
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short term toxicity assessments of an Antibiotic Metabolite in wistar rats and its metabonomics analysis by ultra high performance liquid chromatography coupled to quadrupole time of flight mass spectrometry
Toxicology and Applied Pharmacology, 2016Co-Authors: Hongxing Han, Hailong XiaoAbstract:4-Epi-oxytetracycline (4-EOTC), one of main oxytetracycline (OTC) Metabolites, can be commonly detected in food and environment. The toxicity and effects of OTC on animals have been well characterized; however, its Metabolites have never been studied systemically. This study aims to investigate 15-day oral dose toxicity and urine metabonomics changes of 4-EOTC after repeated administration in Wistar rats at daily doses of 0.5, 5.0 and 50.0mg/kg bw (bodyweight). Hematology and clinical chemistry parameters, including white blood cell count, red blood cell count, total protein, globulin and albumin/globulin, were obviously altered in rats of 5.0 and 50.0mg/kg bw. Histopathology changes of kidney and liver tissues were also observed in high-dose groups. Urinary Metabolites from all groups were analyzed using ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Seventeen Metabolites contributing to the clusters were identified as potential biomarkers from multivariate analysis, including aminoadipic acid, 6-phosphogluconate, sebacic acid, pipecolic acid, etc. The significant changes of these biomarkers demonstrated metabonomic variations in treated rats, especially lysine and purine metabolism. For the first time in this paper, we combined the results of toxicity and metabonomics induced by 4-EOTC for the serious reconsideration of the safety and potential risks of Antibiotics and its degradation Metabolites.
Yansheng Wang - One of the best experts on this subject based on the ideXlab platform.
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transcriptomic analysis reveals new regulatory roles of clp signaling in secondary Metabolite biosynthesis and surface motility in lysobacter enzymogenes oh11
Applied Microbiology and Biotechnology, 2014Co-Authors: Yansheng Wang, Guoliang Qian, Yuxin Zhao, Juan Zhang, Yangyang Zhao, Yan Shen, Justin M Huffman, Vittorio Venturi, Fengquan LiuAbstract:Lysobacter enzymogenes is a bacterial biological control agent emerging as a new source of Antibiotic Metabolites, such as heat-stable antifungal factor (HSAF) and the antibacterial factor WAP-8294A2. The regulatory mechanism(s) for Antibiotic Metabolite biosynthesis remains largely unknown in L. enzymogenes. Clp, a cyclic adenosine monophosphate (cAMP)-receptor-like protein, is shown to function as a global regulator in modulating biocontrol-associated traits in L. enzymogenes. However, the genetic basis of Clp signaling remains unclear. Here, we utilized transcriptome/microarray analysis to determine the Clp regulon in L. enzymogenes. We showed that Clp is a global regulator in gene expression, as the transcription of 775 genes belonging to 19 functional groups was differentially controlled by Clp signaling. Analysis of the Clp regulon detected previously characterized Clp-modulated functions as well as novel loci. These include novel loci involved in Antibiotic Metabolite biosynthesis and surface motility in L. enzymogenes. We further showed experimentally that Clp signaling played a positive role in regulating the biosynthesis of HSAF and WAP-8294A2, as well as surface motility which is a type-IV-pilus-dependent trait. The regulation by Clp signaling of Antibiotic (HSAF and WAP-8294A2) biosynthesis and surface motility was found to be independent. Importantly, we identified a factor Lysobacter acetyltransferase (Lat), a homologue of histone acetyltransferase Hpa2, which was regulated by Clp and involved in HSAF biosynthesis, but not associated with WAP-8294A2 production and surface motility. Overall, our study provided new insights into the regulatory role and molecular mechanism of Clp signaling in L. enzymogenes.