The Experts below are selected from a list of 99261 Experts worldwide ranked by ideXlab platform
Mark Brunswick - One of the best experts on this subject based on the ideXlab platform.
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In vitro Antibody Production.
Current protocols in molecular biology edited by Frederick M. Ausubel ... [et al.], 2001Co-Authors: James J. Mond, Mark BrunswickAbstract:This unit describes the antigenic stimulation of in vitro Antibody Production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro Antibody Production is presented along with a procedure for quantifying the number of Antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described.
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Current Protocols in Molecular Biology - In Vitro Antibody Production
Current Protocols in Molecular Biology, 2000Co-Authors: James J. Mond, Mark BrunswickAbstract:This unit describes the antigenic stimulation of in vitro Antibody Production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro Antibody Production is presented along with a procedure for quantifying the number of Antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described.
James J. Mond - One of the best experts on this subject based on the ideXlab platform.
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In vitro Antibody Production.
Current protocols in molecular biology edited by Frederick M. Ausubel ... [et al.], 2001Co-Authors: James J. Mond, Mark BrunswickAbstract:This unit describes the antigenic stimulation of in vitro Antibody Production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro Antibody Production is presented along with a procedure for quantifying the number of Antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described.
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Current Protocols in Molecular Biology - In Vitro Antibody Production
Current Protocols in Molecular Biology, 2000Co-Authors: James J. Mond, Mark BrunswickAbstract:This unit describes the antigenic stimulation of in vitro Antibody Production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro Antibody Production is presented along with a procedure for quantifying the number of Antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described.
Daisuke Nishimiya - One of the best experts on this subject based on the ideXlab platform.
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Proteins improving recombinant Antibody Production in mammalian cells
Applied Microbiology and Biotechnology, 2014Co-Authors: Daisuke NishimiyaAbstract:Mammalian cells have been successfully used for the industrial manufacture of antibodies due to their ability to synthesize antibodies correctly. Nascent polypeptides must be subjected to protein folding and assembly in the ER and the Golgi to be secreted as mature proteins. If these reactions do not proceed appropriately, unfolded or misfolded proteins are degraded by the ER-associated degradation (ERAD) pathway. The accumulation of unfolded proteins or intracellular Antibody crystals accompanied by this failure triggers the unfolded protein response (UPR), which can considerably attenuate the levels of translation, folding, assembly, and secretion, resulting in reduction of Antibody productivity. Accumulating studies by omics-based analysis of recombinant mammalian cells suggest that not only protein secretion processes including protein folding and assembly but also translation are likely to be the rate-limiting factors for increasing Antibody Production. Here, this review describes the mechanism of Antibody folding and assembly and recent advantages which could improve recombinant Antibody Production in mammalian cells by utilizing proteins such as ER chaperones or UPR-related proteins.
Yanming Wang - One of the best experts on this subject based on the ideXlab platform.
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extracellular matrix protein 1 promotes follicular helper t cell differentiation and Antibody Production
Proceedings of the National Academy of Sciences of the United States of America, 2018Co-Authors: Meng Wang, Xiaoyan Chang, Xiaoyu Sun, Xuan Lin, Chenghua Yan, Weiguo Fan, Yanming WangAbstract:T-follicular helper (TFH) cells are a subset of CD4+ helper T cells that help germinal center (GC) B-cell differentiation and high-affinity Antibody Production during germinal center reactions. Whether important extracellular molecules control TFH differentiation is not fully understood. Here, we demonstrate that a secreted protein extracellular matrix protein 1 (ECM1) is critical for TFH differentiation and Antibody response. A lack of ECM1 inhibited TFH cell development and impaired GC B-cell reactions and antigen-specific Antibody Production in an antigen-immunized mouse model. ECM1 was induced by IL-6 and IL-21 in TFH cells, promoting TFH differentiation by down-regulating the level of STAT5 phosphorylation and up-regulating Bcl6 expression. Furthermore, injection of recombinant ECM1 protein into mice infected with PR8 influenza virus promoted protective immune responses effectively, by enhancing TFH differentiation and neutralizing Antibody Production. Collectively, our data identify ECM1 as a soluble protein to promote TFH cell differentiation and Antibody Production.
Gyun Min Lee - One of the best experts on this subject based on the ideXlab platform.
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EFFECT OF HYPOOSMOTIC STRESS ON HYBRIDOMA CELL GROWTH AND Antibody Production
Biotechnology and bioengineering, 1997Co-Authors: Joon Soo Ryu, Gyun Min LeeAbstract:To investigate the response of hybridoma cells to hypoosmotic stress, S3H5/γ2bA2 and DB9G8 hybridomas were cultivated in the hypoosmolar medium (DMEM supplemented with 10% serum) resulting from sodium chloride subtraction. Both hybridomas showed similar responses to hypoosmotic stress in regard to cell growth and Antibody Production. The cell growth and Antibody Production at 276 mOsm/kg were comparable to those at 329 mOsm/kg (standard DMEM). Both cells grew well at 219 mOsm/kg, though their growth and Antibody Production were slightly decreased. When the osmolality was further decreased to 168 mOsm/kg, the cell growth did not occur. When subjected to hyperosmotic stress, both cells displayed significantly enhanced specific Antibody productivity (q Ab). However, the cells subjected to hypoosmotic stress did not display enhanced q Ab. Taken together, both hyperosmotic and hypoosmotic stresses depressed the growth of S3H5/y2bA2 and DB9G8 hybridomas. However, their response to hypoosmotic stress in regard to q Ab was different from that to hyperosmotic stress.
