The Experts below are selected from a list of 53655 Experts worldwide ranked by ideXlab platform
Michiko Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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Antibody Production in Human Tonsil—Detection of Antibody Forming Cell, and Antigen Binding Cell to Dermatophagoides Farinae
Auris Nasus Larynx, 2013Co-Authors: Takeru Ishikawa, Tetsuo Shimada, Hisao Miyashita, Michiko IshikawaAbstract:Antibody production of Dermatophagoides farinae (mite) in human tonsil are observed by means of plaque-forming Cells (PFC), and radioimmuno assay without any in vitro stimulation. Tonsil tissues donated by patients allergic to mite are used for the experimental work. Antibody titer of sera from these patients is estimated by a hemagglutination test. Isolated tonsil lymphocytes are used for estimation of T, and B subpopulation. Direct, and indirect PFC assays are performed on human O-type red Cells coupled with mite Antigen. 131 I labelled mite Antigen reacts with lymphocytes, and Antigen Binding Cells are observed radioautographically. T Cell, and B Cell populations are 19.5–34.2, and 39.5–66.0 % respectively. Number of PFC in 2 × 10 6 Cells are 3.2-9.2 × 10 2 . Radioautographic pictures are also demonstrated. In conclusion, palatine tonsils trap the mite Antigen, inhalating foreign bodies, and actively produce antibodies.
Takeru Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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Antibody Production in Human Tonsil—Detection of Antibody Forming Cell, and Antigen Binding Cell to Dermatophagoides Farinae
Auris Nasus Larynx, 2013Co-Authors: Takeru Ishikawa, Tetsuo Shimada, Hisao Miyashita, Michiko IshikawaAbstract:Antibody production of Dermatophagoides farinae (mite) in human tonsil are observed by means of plaque-forming Cells (PFC), and radioimmuno assay without any in vitro stimulation. Tonsil tissues donated by patients allergic to mite are used for the experimental work. Antibody titer of sera from these patients is estimated by a hemagglutination test. Isolated tonsil lymphocytes are used for estimation of T, and B subpopulation. Direct, and indirect PFC assays are performed on human O-type red Cells coupled with mite Antigen. 131 I labelled mite Antigen reacts with lymphocytes, and Antigen Binding Cells are observed radioautographically. T Cell, and B Cell populations are 19.5–34.2, and 39.5–66.0 % respectively. Number of PFC in 2 × 10 6 Cells are 3.2-9.2 × 10 2 . Radioautographic pictures are also demonstrated. In conclusion, palatine tonsils trap the mite Antigen, inhalating foreign bodies, and actively produce antibodies.
Tetsuo Shimada - One of the best experts on this subject based on the ideXlab platform.
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Antibody Production in Human Tonsil—Detection of Antibody Forming Cell, and Antigen Binding Cell to Dermatophagoides Farinae
Auris Nasus Larynx, 2013Co-Authors: Takeru Ishikawa, Tetsuo Shimada, Hisao Miyashita, Michiko IshikawaAbstract:Antibody production of Dermatophagoides farinae (mite) in human tonsil are observed by means of plaque-forming Cells (PFC), and radioimmuno assay without any in vitro stimulation. Tonsil tissues donated by patients allergic to mite are used for the experimental work. Antibody titer of sera from these patients is estimated by a hemagglutination test. Isolated tonsil lymphocytes are used for estimation of T, and B subpopulation. Direct, and indirect PFC assays are performed on human O-type red Cells coupled with mite Antigen. 131 I labelled mite Antigen reacts with lymphocytes, and Antigen Binding Cells are observed radioautographically. T Cell, and B Cell populations are 19.5–34.2, and 39.5–66.0 % respectively. Number of PFC in 2 × 10 6 Cells are 3.2-9.2 × 10 2 . Radioautographic pictures are also demonstrated. In conclusion, palatine tonsils trap the mite Antigen, inhalating foreign bodies, and actively produce antibodies.
Hisao Miyashita - One of the best experts on this subject based on the ideXlab platform.
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Antibody Production in Human Tonsil—Detection of Antibody Forming Cell, and Antigen Binding Cell to Dermatophagoides Farinae
Auris Nasus Larynx, 2013Co-Authors: Takeru Ishikawa, Tetsuo Shimada, Hisao Miyashita, Michiko IshikawaAbstract:Antibody production of Dermatophagoides farinae (mite) in human tonsil are observed by means of plaque-forming Cells (PFC), and radioimmuno assay without any in vitro stimulation. Tonsil tissues donated by patients allergic to mite are used for the experimental work. Antibody titer of sera from these patients is estimated by a hemagglutination test. Isolated tonsil lymphocytes are used for estimation of T, and B subpopulation. Direct, and indirect PFC assays are performed on human O-type red Cells coupled with mite Antigen. 131 I labelled mite Antigen reacts with lymphocytes, and Antigen Binding Cells are observed radioautographically. T Cell, and B Cell populations are 19.5–34.2, and 39.5–66.0 % respectively. Number of PFC in 2 × 10 6 Cells are 3.2-9.2 × 10 2 . Radioautographic pictures are also demonstrated. In conclusion, palatine tonsils trap the mite Antigen, inhalating foreign bodies, and actively produce antibodies.
R. Ashman - One of the best experts on this subject based on the ideXlab platform.
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THE SECRET LIFE OF THE Antigen-Binding Cell
Strategies of Immune Regulation, 2013Co-Authors: R. AshmanAbstract:Publisher Summary Immunologists have compiled an impressive list of interaction mechanisms based on the traditional methodology, in which lymphocyte subpopulations are identified and isolated by means of surface markers, and their functions defined in recombination experiments. In the models of the immune response so derived, the poor B Cell commonly appears last, being triggered only if Antigen has first successfully interacted with the proper T Cells and macrophages. It is important, however, to realize that failure to generate plaque-forming Cells cannot be taken as evidence of Antigen's failure to trigger the Antigen-Binding Cell. In fact, the available data still permits a direct triggering model in which Antigen contact with the B Cell results in a normal activation signal, plus some additional early events, with T helper Cell and macrophage signals impacting at a later step. To fully test such a model, it would be necessary to define several critical early events occurring in the B Cell after Antigen contact. Thus, attention must be focused on that small population of Cells that carry specific receptors for the Antigen used to stimulate the Antigen-Binding Cells (ABC). Evidence already exists that favors some form of direct triggering model, including the demonstration that proliferation of B Cells may result from direct Antigen contact in the absence of T Cells and that B memory may be generated in the absence of T Cells.
