The Experts below are selected from a list of 1059 Experts worldwide ranked by ideXlab platform

Margaret A Brimble - One of the best experts on this subject based on the ideXlab platform.

Jack L Y Chen - One of the best experts on this subject based on the ideXlab platform.

Magdalena Litaay - One of the best experts on this subject based on the ideXlab platform.

  • The Potency of ??-Sitosterol Isolated From Hydroid Aglaophenia cupressina Lamoureoux as Antimitotic Agent towards Seatowards Sea Urchin Tripneustes gratilla Linn.
    2017
    Co-Authors: Eva Johannes, Magdalena Litaay
    Abstract:

    The potency of ??-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as\ud Antimitotic Agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been\ud conducted. The study aimed to determine ??-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as Antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were ??-Sitosterol (concentration 1 ??g/ml, 10 ??g/ml and100??g/ml), positive control vincristine (concentration 0.01 ??g/ml, 0.1 ??g/ml, and 1 ??g/ml) and negative control. A protozoa-free seawater was used in this assay. The results revealed that ??-Sitosterol compound had the highest inhibition activity at concentration of 100 ??g/ml was 69.22 % with IC-50 on 0.95 ??g/ml which could develop as cancer chemotherapy compound. Keywords: Antimitotic, ??-Sitosterol, Hydroid Aglaophenia cupressina Lamoureoux, Tripneustes gratilla\ud Linn

  • the potency of β sitosterol isolated from hydroid aglaophenia cupressina lamoureoux as Antimitotic Agent towards sea urchin tripneustes gratilla linn
    International Journal of Engineering, 2017
    Co-Authors: Afriansyah Afriansyah, Eva Johannes, Sjafaraenan Sjafaraenan, Magdalena Litaay
    Abstract:

    The potency of β-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as Antimitotic Agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been conducted. The study aimed to determine β-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as Antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were β-Sitosterol (concentration 1 µg/ml, 10 µg/ml and100 µg/ml), positive control vincristine (concentration 0.01 µg/ml, 0.1 µg/ml, and 1 µg/ml) and negative control.  A protozoa-free seawater was used in this assay. The results revealed that β-Sitosterol compound had the highest inhibition activity at concentration of 100 μg/ml was 69.22 % with IC-50 on 0.95 µg/ml which could develop as cancer chemotherapy compound.

Ernest Hamel - One of the best experts on this subject based on the ideXlab platform.

  • synthesis Antimitotic and antivascular activity of 1 3 4 5 trimethoxybenzoyl 3 arylamino 5 amino 1 2 4 triazoles
    Journal of Medicinal Chemistry, 2014
    Co-Authors: Romeo Romagnoli, Maria Kimatrai Salvador, Andrea Brancale, Ernest Hamel, Pier Giovanni Baraldi, Filippo Prencipe, Valerio Bertolasi, Michela Cancellieri, Ignazio Castagliuolo, Francesca Consolaro
    Abstract:

    A new class of compounds that incorporated the structural motif of the 1-(3′,4′,5′-trimethoxtbenzoyl)-3-arylamino-5-amino-1,2,4-triazole molecular skeleton was synthesized and evaluated for their antiproliferative activity in vitro, interactions with tubulin, and cell cycle effects. The most active Agent, 3c, was evaluated for antitumor activity in vivo. Structure–activity relationships were elucidated with various substituents on the phenyl ring of the anilino moiety at the C-3 position of the 1,2,4-triazole ring. The best results for inhibition of cancer cell growth were obtained with the p-Me, m,p-diMe, and p-Et phenyl derivatives 3c, 3e, and 3f, respectively, and overall, these compounds were more or less as active as CA-4. Their vascular disrupting activity was evaluated in HUVEC cells, with compound 3c showing activity comparable with that of CA-4. Compound 3c almost eliminated the growth of syngeneic hepatocellular carcinoma in Balb/c mice, suggesting that 3c could be a new Antimitotic Agent with c...

  • synthesis and evaluation of 1 5 disubstituted tetrazoles as rigid analogues of combretastatin a 4 with potent antiproliferative and antitumor activity
    Journal of Medicinal Chemistry, 2012
    Co-Authors: Romeo Romagnoli, Delia Preti, Maria Kimatrai Salvador, Andrea Brancale, Suzhan Zhang, Xianhua Fu, Mojgan Aghazadeh Tabrizi, Pier Giovanni Baraldi, Ernest Hamel
    Abstract:

    Tubulin, the major structural component of microtubules, is a target for the development of anticancer Agents. Two series of 1,5-diaryl substituted 1,2,3,4-tetrazoles were concisely synthesized, using a palladium-catalyzed cross-coupling reaction, and identified as potent antiproliferative Agents and novel tubulin polymerization inhibitors that act at the colchicine site. SAR analysis indicated that compounds with a 4-ethoxyphenyl group at the N-1 or C-5 position of the 1,2,3,4-tetrazole ring exhibited maximal activity. Several of these compounds also had potent activity in inhibiting the growth of multidrug resistant cells overexpressing P-glycoprotein. Active compounds induced apoptosis through the mitochondrial pathway with activation of caspase-9 and caspase-3. Furthermore, compound 4l significantly reduced in vivo the growth of the HT-29 xenograft in a nude mouse model, suggesting that 4l is a promising new Antimitotic Agent with clinical potential.

  • synthesis x ray crystal structure and tubulin binding properties of a benzofuran analogue of the potent cytotoxic Agent combretastatin a4
    Australian Journal of Chemistry, 1999
    Co-Authors: Martin G Banwell, Bernard L Flynn, Anthony C Willis, Ernest Hamel
    Abstract:

    The benzofuran (4), a ring-fused analogue of the potent Antimitotic Agent combretastatin A4 (1), has been prepared by a convergent route involving 5-endo-dig iodocyclization of o-hydroxytolan (5) as the key step. Compound (4), which has been characterized crystallographically as well as spectroscopically, is inactive as a tubulin-binding Agent.

  • Curacin D, aN Antimitotic Agent from the marine Cyanobacterium Lyngbya majuscula
    Phytochemistry, 1998
    Co-Authors: Brian L. Marquez, Ernest Hamel, Pascal Verdier-pinard, William H. Gerwick
    Abstract:

    Curacin D is a novel brine shrimp toxic metabolite isolated from a Virgin Islands collection of the marine cyanobacterium Lyngbya majuscula. Structure elucidation of curacin D was accomplished through multidimensional NMR, GC/MS, and comparisons with curacin A. Curacin D provides new insights into structure-activity relationships in this natural product class as well as some aspects of the likely biosynthetic pathway of the curacins.

  • structure of curacin a a novel Antimitotic antiproliferative and brine shrimp toxic natural product from the marine cyanobacterium lyngbya majuscula
    Journal of Organic Chemistry, 1994
    Co-Authors: William H. Gerwick, Ernest Hamel, Dale G. Nagle, Philip Proteau, Andrei V Blokhin, Doris L Slate
    Abstract:

    Bioassay-guided fractionation of the organic extract of a Curacao collection of Lyngbya majuscula led to the isolation of a new lipid, curacin A, with exceptional brine shrimp toxic and antiproliferative activities. Its unique thiazoline-containing structure has been deduced from spectroscopic information. Pure curacin A is an Antimitotic Agent (IC 50 values in three cell lines ranging from 7 to 200 nM) that inhibits microtubule assembly and the binding of colchicine to tubulin

William H. Gerwick - One of the best experts on this subject based on the ideXlab platform.